Proline derivatives and use thereof as drugs

(57) Abrégé/Abstract:

The present invention aims at providing compounds having therapeutic effects due to a DPP-IV inhibitory action, and satisfactory as pharmaceutical products. The present inventors have found that derivatives having a substituent introduced into the Y-position

Canada http://opic.gc.ca · Ottawa-Hull KIA 0C9 · http://cipo.gc.ca

OPIC-CIPO 191

O PI c

<img file="CA2418656C_D0001.tif" />

CA 2418656 C 2011/02/01 (57) Abrégé(suite)/Abstract(continued):

of proline represented by the formula (I) (see formula I) wherein each symbol is as defined in the specification, have a potent DPPIV inhibitory activity, and completed the present invention by increasing the stability.

(11)(21) 2 418 656 (13) C

-2CA 02418656 2003-02-06

<img file="CA2418656C_D0002.tif" />

Abstract

The present invention aims at providing compounds having therapeutic effects due to a DPP-IV inhibitory action, and satisfactory as pharmaceutical products.

The present inventors have found that derivatives having a substituent introduced into the γ-position of proline represented by the formula (I)

HN (I) io wherein each symbol is as defined in the specification, have a potent DPP-IV inhibitory activity, and completed the present invention by increasing the stability.

337

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JUMBO APPLICATIONS / PATENTS

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CA 02418656 2003-02-06

DESCRIPTION

PROLINE DERIVATIVES AND USE THEREOF AS DRUGS Technical Field

The present invention relates to a proline derivative showing a dipeptidyl peptidase IV (DPP-IV) inhibitory activity, which is useful for the treatment or prophylaxis of diabetes, obesity, HIV infection, cancer metastasis, dermopathy, prostatic hyperplasia, periodontitis, autoimmune disease and the like, and a salt thereof.

Background Art

DPP-IV is a serine protease, which recognizes an amino acid sequence having proline (or alanine or hydroxyproline) the penultimate position from the N-terminal and produces dipeptide Xaa-Pro (Xaa shows an optional amino acid and Pro shows Lproline). DPP-IV is widely distributed in mammalian tissues and is known to be present particularly in blood, kidney, intestinal epithelium and placenta.

While the physiological role of DPP-IV in mammal has not been completely elucidated, its involvement in a broad range of functions of living organisms such as degradation of neuropeptide [Heymann et al., FEBS Letters vol. 91, 360-364 (1978)], activation of T cell [Schon et al., Biomedica Biochimica Acta vol. 44, K9-K15 (1985)], adhesion of metastatic tumor cell to endothelium [Johnson et al., Journal of Cell Biology, vol. 121, 1423-1432 (1993)], invasion of HIV virus into lymphocytes [Callebaut et al., Science vol. 262, 2045-2050 (1993)] and the like is being clarified. Of these, the role of DPP-IV as an enzyme that inactivates glucagon-like peptide (GLP-1), which is a biogenic substance having a strong insulin secretion ability and controls postprandial blood glucose level, has been drawing attention [Deacon et al., Journal of Clinical Endocrinology and Metabolism, vol. 80, 952-957 (1995)].

GLP-1 is known to be metabolized in several minutes in a

CA 02418656 2003-02-06 living organism. In the metabolism, that by DPP-IV is particularly important, because it quickly cleaves GLP-1 and produces inert GLP-1 [Deacon et al., American Journal of

Physiology, vol. 271, E458-E464 (1996)]. In addition, it is considered that physiological action of GLP-1 becomes attenuated further because this inert GLP-1 shows an antagonistic action on GLP-1 receptor [Knudsen et al., European Journal of Pharmacology, vol. 318, 429-435 (1996)]. Therefore, a method for suppressing cleavage of GLP-1 by inhibition of io DPP-IV is considered to be the most superior approach for reinforcing GLP-1 action. That is, a DPP-IV inhibitor is expected to be a superior treatment method of curing postprandial hyperglycemia without side effects, such as prolonged hypoglycemia and the like, for non insulin-dependent is diabetic (type II diabetes) patients.

Patent applications relating to DPP-IV inhibitors include the following.

Japanese Patent Application under PCT laid-open under kohyo No. 9-509921 discloses (S)-2-cyano-1-L-prolinepyrrolidine 20 derivative. The L-a-amino acid corresponding to the L-proline moiety of the compound disclosed therein characteristically has a lipophilic side chain.

In addition, WO99/61431 describes DPP-IV inhibitory activities of a compound consisting of natural amino acid and 25 thiazolidine or pyrrolidine.

Disclosure of the Invention

While there have been reported many DPP-IV inhibitors till date [Augustyns et al., Current Medicinal Chemistry, vol. 6, 311-327 (1999)], none of the compounds shows sufficient inhibitory activity or sufficient stability and safety in living organisms, and they are unsatisfactory as a pharmaceutical product. Therefore, the development of a compound which exhibits a therapeutic effect through the

CA 02418656 2003-02-06 inhibition of DPP-IV action and satisfactory as a pharmaceutical product has been demanded.

In view of the above-mentioned points, the present inventors have conducted intensive studies with the aim of developing a novel DPP-IV inhibitor. As a result, the present inventors have found that a derivative having a substituent introduced into the γ-position of proline has a potent DPP-IV inhibitory activity, and made the stability higher, which resulted in the completion of the present invention.

Accordingly, the present invention relates to the following compounds.

(1) An L-proline derivative of the formula (I)

<img file="CA2418656C_D0003.tif" />

wherein

X shows -NR^XR² wherein R^x and R² may be the same or different and each is independently a hydrogen atom, alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl or heteroarylalkyl, or may be bonded to each other to form a heterocycle optionally containing 1 or 2 nitrogen atoms or oxygen atoms, the heterocycle optionally being condensed with an aromatic ring optionally having substituents, and the heterocycle optionally being a spiro ring,

-NR³COR⁴ wherein R³ and R⁴ are the same or different and each is independently a hydrogen atom, alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, arylalkenyl, heteroaryl or heteroarylalkyl,

-NR⁵ CONR⁶ R⁷ or -NR⁵ CH₂CH₂NR⁶ R⁷ wherein R⁵, R⁶ and R⁷ are

CA 02418656 2003-02-06 the same or different and each is independently hydrogen atom, alkyl, acyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl or heteroarylalkyl, or R⁶ and R⁷ may be bonded to each other to form a heterocycle optionally containing 1 or 2 nitrogen atoms or oxygen atoms, the heterocycle optionally being condensed with an aromatic ring optionally having substituents,

-NR⁸ SO₂ R⁹ wherein R⁸ and R⁹ are the same or different and each is independently a hydrogen atom, alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl or heteroarylalkyl, or

-OR¹⁰ or -OCOR¹¹ wherein R¹⁰ and R¹¹ are each a hydrogen atom, alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl or heteroarylalkyl,

Y is CH₂ , CH-OH, S, S=O or SO₂,

Z is a hydrogen atom or a cyano, and of the above-mentioned groups, alkyl, aryl, arylalkyl, arylalkenyl, heteroaryl, heteroarylalkyl, cycloalkyl, cycloalkylalkyl and heterocycle each optionally have substituents, or a pharmaceutically acceptable salt thereof.

(2) The L-proline derivative described in the aforementioned (1), wherein X of thé formula (I) is a substituent selected from the formulas

<img file="CA2418656C_D0004.tif" />

wherein (II)

<img file="CA2418656C_D0005.tif" />

______ is a single bond or a double bond,

R¹² is alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl,

CA 02418656 2003-02-06 heteroaryl, heteroarylalkyl, -NR¹⁴R¹⁵, -OR¹⁶, -COR¹⁷, -CO2 R¹⁸ , -CONR¹⁹ R^{2 0} or -SO2 R²¹CH₂ wherein R¹⁴ , R¹⁵ , R¹⁶ , R¹⁷ , R¹⁸ , R¹⁹ , R^{2 0} and R²¹ are the same or different and each is independently a hydrogen atom, alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl or haloalkyl, or R¹⁴ and R¹⁵, and R¹⁹and R²⁰ may be bonded to each other to form heterocycles each optionally containing 1 or 2 nitrogen atoms or oxygen atoms, said heterocycle optionally being condensed with an aromatic ring optionally having substituents,

R¹³ is a hydrogen atom, alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl or heteroarylalkyl, m is 1 or 2, and

A is a carbon atom or a nitrogen atom, provided that i) when A is a carbon atom, A may be substituted by a hydroxyl group, carboxyl or alkoxycarbonyl, and ii) when A is a nitrogen atom, ______ is a single bond, of the above-mentioned groups, alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl and heterocycle each optionally have substituents, or a pharmaceutically acceptable salt thereof.

(3) The L-proline derivative described in the aforementioned (1) or (2), wherein X of the formula (I) is a substituent selected from the formulas

CA 02418656 2003-02-06 p22 __ | R23 “—N—-(CH^,,---G 'c (Π)

Vd R²⁴

<img file="CA2418656C_D0006.tif" />

<td> Y</td><td> =b r23 a -d ^R</td>

<td> r\ _/Γ</td><td></td>

<td> )=</td><td></td>

<td> % f-</td><td> F —e</td>

R^{2 2} is a hydrogen atom, alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl or heteroarylalkyl,

R^{2 3} and R^{2 4} are the same or different and each is independently a hydrogen atom, alkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl, halogen, haloalkyl, cyano, nitro, -NR^{2 5} R^{2 6}, -NHSO2 R^{2 7}, -OR^{2 8}, -COOR^{2 9}, -CONHSO2 R^{3 0}, -SO2 OR³¹ , -SO2 R^{3 2} or -CONR^{3 3} R^{3 4} wherein R^{2 5}, R^{2 6} , R^{2 7}, R^{2 8}, R^{2 9} , R^{3 0}, R³¹, R^{3 2}, R^{3 3} and R^{3 4} are the same or different and each is independently a hydrogen atom, alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl or haloalkyl, or R^{2 5} and R^{2 6}, and R^{3 3} and R^{3 4} may be bonded to each other to form heterocycles each optionally containing 1 or 2 nitrogen atoms or oxygen atoms, said heterocycle optionally being condensed with an aromatic ring optionally having substituents, a, b, c, d, e, f and g are all carbon atoms, or any one or two thereof is(are) nitrogen atom(s) and the rest is a carbon atom, n is 0, 1, 2 or 3, and

A is a carbon atom or a nitrogen atom, provided that when A is a carbon atom, A may be substituted by a hydroxyl group, carboxyl or alkoxycarbonyl, and of the above-mentioned groups, alkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl, cycloalkyl, cycloalkylalkyl and

CA 02418656 2010-09-29

27103-383 heterocycle each optionally have substituents, or a pharmaceutically acceptable salt thereof.

(4) The L-proline derivative of any of the aforementioned (1) to (3), wherein, in the formula (I), the asymmetric carbon, to which X is bonded, is expressed by an

S configuration, X is a group of the formula (VI) or (VII), R²³ and R²⁴ are the same or different and each is nitro, cyano, C1.6 alkyl, C1-6 alkyloxy, halogen or haloalkyl, Y is a sulfur atom and Z is a hydrogen atom, or a pharmaceutically acceptable salt thereof.

(5) The L-proline derivative of the aforementioned (1), wherein, in the formula (I),

X is a hydroxyl group, phenylamino optionally having substituents, 2-pyridylamino optionally having substituents, 3-pyridazinylamino optionally having substituents or 2-pyrimidinylamino optionally having substituents, and the asymmetric carbon, to which X is bonded, is expressed by an S configuration, or a pharmaceutically acceptable salt thereof.

(6) A compound of the formula (I) as shown above in the aforementioned (1), wherein

X is a substituent selected from the formulas

<img file="CA2418656C_D0007.tif" />

(II)

<img file="CA2418656C_D0008.tif" />

...... is a single bond or a double bond,

R¹² is alkyl having 1 to 8 carbon atoms, cycloalkyl having 3 to 7 carbon atoms, cycloalkylalkyl wherein the cycloalkyl moiety has 3 to 7 carbon atoms and the alkyl moiety has 1 to 3 carbon atoms,

CA 02418656 2010-09-29

27103-383 aryl which is selected from phenyl, naphthyl and an ortho fused bicyclic group having 8 to 10 ring atoms wherein at least one ring is an aromatic ring, arylalkyl having an aryl moiety as defined above and an alkyl moiety having 1 to 3 carbon atoms, heteroaryl selected from a 5 or 6-membered ring group having carbon atoms and 1-4 hetero atoms selected from oxygen, sulfur and nitrogen, and an ortho fused bicyclic heteroaryl having 8 to 10 ring atoms which is derived therefrom, heteroarylalkyl having a heteroaryl moiety as defined above and an alkyl moiety having 1 to 3 carbon atoms,

-NR¹⁴R¹⁵,

-OR¹⁶,

-COR¹⁷,

-CO₂R¹⁸,

-CONR¹⁹R²⁰ or

-SO₂R²¹ wherein R¹⁴, R¹⁵, R¹⁶, R¹⁷, R¹⁸, R¹⁹, R²⁰ and R²¹ are the same or different and each is independently a hydrogen atom, alkyl having 1 to 8 carbon atoms, cycloalkyl having 3 to 7 carbon atoms, cycloalkylalkyl wherein the cycloalkyl moiety has 3 to 7 carbon atoms and the alkyl moiety has 1 to 3 carbon atoms,

CA 02418656 2010-09-29

27103-383 aryl which is selected from phenyl, naphthyl and an ortho fused bicyclic group having 8 to 10 ring atoms wherein at least one ring is an aromatic ring, arylalkyl having an aryl moiety as defined above and an alkyl moiety having 1 to 3 carbon atoms, heteroaryl selected from a 5 or 6-membered ring group having carbon atoms and 1-4 hetero atoms selected from oxygen, sulfur and nitrogen, and an ortho fused bicyclic heteroaryl having 8 to 10 ring atoms which is derived therefrom, heteroarylalkyl having a heteroaryl moiety as defined above and an alkyl moiety having 1 to 3 carbon atoms, or haloalkyl selected from trifluoromethyl, 2,2,2-trifluoroethyl and pentafluoroethyl, or

R¹⁴ and R¹⁵, and R¹⁹ and R²⁰ may be bonded to each other to form heterocycles selected from a saturated or unsaturated monocyclic 4- to 7-membered ring and a saturated and unsaturated 10- or 11-membered spiro ring each optionally containing 1 or 2 nitrogen atoms or oxygen atoms, said heterocycle optionally being condensed with an aromatic ring selected from benzene ring and pyridine ring optionally having substituents,

R¹³ is a hydrogen atom, alkyl having 1 to 8 carbon atoms, cycloalkyl having 3 to 7 carbon atoms, cycloalkylalkyl wherein the cycloalkyl moiety has 3 to 7 carbon atoms and the alkyl moiety has 1 to 3 carbon atoms, aryl which is selected from phenyl, naphthyl and an ortho fused bicyclic group having 8 to 10 ring atoms wherein at least one ring is an aromatic ring,

CA 02418656 2010-09-29

27103-383 arylalkyl having an aryl moiety as defined above and an alkyl moiety having 1 to 3 carbon atoms, heteroaryl selected from a 5 or 6-membered ring group having carbon atoms and 1-4 hetero atoms selected from oxygen, sulfur and nitrogen, and an ortho fused bicyclic heteroaryl having 8 to 10 ring atoms which is derived therefrom, or heteroarylalkyl having a heteroaryl moiety as defined above and an alkyl moiety having 1 to 3 carbon atoms, m is 1 or 2, and

A is a carbon atom or a nitrogen atom;

provided that i) when A is a carbon atom, A may be substituted by a hydroxyl group, carboxyl or alkoxycarbonyl, and ii) when A is a nitrogen atom,

......is a single bond,

Y is CH₂, CH-OH, S, S=O or SO₂,

Z is a hydrogen atom or a cyano, and as defined above, alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl and heterocycle, each optionally having 1 or more substituent(s) selected from halogen, hydroxyl group, nitro, cyano, trifluoromethyl, alkyl (as defined above), alkoxy, alkylthio, formyl, acyloxy, oxo, phenyl, 2-pyridyl, 3-pyridyl, 4-pyridyl, 4-piperidinyl, 2-morpholinoethyl, 3-picolyl, arylalkyl (as defined above), -COOR_a, -CH₂COOR_a, -OCH₂COOR_a, -CONR_bR_c, -CH₂CQNR_bR_c (Q is =0 or =S), -OCH₂CONR_bR_c, -COO(CH₂)₂NR_eRf, -SO₂T_1t -CONRdSO₂Ti, -NR_eRf, -NR_gCHO, -NR_gCOT₂, -NR_gCOOT₂, -NRgCONRjRj, -NR_kSO₂T₃, -SO₂NR,R_m, -SO₂NR_nCOT₄, methylenedioxy and ethyleneoxy, wherein alkoxy has 1 to 8 carbon atoms, alkylthio has 1 to 8 carbon atoms, and acyloxy has 1 to 8 carbon atoms, and

9a

CA 02418656 2010-09-29

27103-383 wherein R_a-R_n is hydrogen, alkyl (as defined above) or arylalkyl (as defined above), or

R_b and R_c, R_e and Rf, R, and Rj, and R| and R_m of -NR_bRc, -NR_eRf,

-NRjRj and -NR|R_m may be respectively bonded to each other to form heterocycles as defined above each optionally having 1 or 2 nitrogen atoms or oxygen atoms, and said heterocycle may be condensed with an aromatic ring as defined above optionally substituted by the substituents as defined above, or -NR_eRf may be a heteroaryl having =0, and

T-ι - T₄ is selected from hydrogen atom, and as defined above, alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl and haloalkyl, each of which may be substituted by the substituents as defined above, or a pharmaceutically acceptable salt thereof.

(7) A compound of the formula (l-a)

<img file="CA2418656C_D0009.tif" />

( l-a ) wherein

X shows -NR¹R² wherein R¹ and R² may be the same or different and each is independently a hydrogen atom, alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl or heteroarylalkyl, or may be bonded to each other to form a heterocycle optionally containing 1 or 2 nitrogen atoms or oxygen atoms, the heterocycle optionally being condensed with an aromatic ring optionally having substituents, and the heterocycle optionally being a spiro ring,

-NR³COR⁴ wherein R³ and R⁴ are the same or different and each is independently a hydrogen atom, alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, arylalkenyl, heteroaryl or heteroarylalkyl,

9b

CA 02418656 2010-09-29

27103-383

-NR⁵CONR⁶R⁷ or -NR⁵CH₂CH₂NR⁶R⁷ wherein R⁵, R⁶ and R⁷ are the same or different and each is independently a hydrogen atom, alkyl, acyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl or heteroarylalkyl, or R⁶ and R⁷may be bonded to each other to form a heterocycle optionally containing 1 or 2 nitro atoms or oxygen atoms, said heterocycle optionally being condensed with an aromatic ring optionally having substituents, or

-NR⁸SO₂R⁹ wherein R⁸ and R⁹ are the same or different and each is independently a hydrogen atom, alkyl cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl or heteroarylalkyl,

Y is CH₂, CH-OH, S, S=O or SO₂,

Z is a hydrogen atom or a cyano,

R³⁵ is -COR⁴¹ and R⁴¹ is a hydrogen atom, alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl or heteroarylalkyl, or -COOR⁴² wherein R⁴² is alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl or heteroarylalkyl, and of the above-mentioned groups, alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, arylalkenyl, heteroaryl, heteroarylalkyl and heterocycle each optionally have substituents.

(8) A compound of the formula (l-a), as shown above in the aforementioned (7), wherein X, Y and Z are as defined in the aforementioned (6) and

R³⁵ is -COR⁴¹ wherein R⁴¹ is a hydrogen atom, alkyl having 1 to 8 carbon atoms, cycloalkyl having 3 to 7 carbon atoms, cycloalkylalkyl wherein the cycloalkyl moiety has 3 to 7 carbon atoms and the alkyl moiety has 1 to 3 carbon atoms,

9c

CA 02418656 2010-09-29

27103-383 aryl which is selected from phenyl, naphthyl and an ortho fused bicyclic group having 8 to 10 ring atoms wherein at least one ring is an aromatic ring, arylalkyl having an aryl moiety as defined above and an alkyl moiety having 1 to 3 carbon atoms, heteroaryl selected from a 5 or 6-membered ring group having carbon atoms and 1-4 hetero atoms selected from oxygen, sulfur and nitrogen, and an ortho fused bicyclic heteroaryl having 8 to 10 ring atoms which is derived therefrom, or heteroarylalkyl having a heteroaryl moiety as defined above and an alkyl moiety having 1 to 3 carbon atoms, or

-COOR⁴² wherein R⁴² is alkyl having 1 to 8 carbon atoms, cycloalkyl having 3 to 7 carbon atoms, cycloalkylalkyl wherein the cycloalkyl moiety has 3 to 7 carbon atoms and the alkyl moiety has 1 to 3 carbon atoms, aryl which is selected from phenyl, naphthyl and an ortho fused bicyclic group having 8 to 10 ring atoms wherein at least one ring is an aromatic ring, arylalkyl having an aryl moiety as defined above and an alkyl moiety having 1 to 3 carbon atoms, heteroaryl selected from a 5 or 6-membered ring group having carbon atoms and 1-4 hetero atoms selected from oxygen, sulfur and nitrogen, and an ortho fused bicyclic heteroaryl having 8 to 10 ring atoms which is derived therefrom, or

9d

CA 02418656 2010-09-29

27103-383 heteroarylalkyl having a heteroaryl moiety as defined above and an alkyl moiety having 1 to 3 carbon atoms, and as defined above, alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl and heteroarylalkyl, each optionally having 1 or more substituent(s) selected from halogen, hydroxyl group, nitro, cyano, trifluoromethyl, alkyl (as defined above), alkoxy, alkylthio, formyl, acyloxy, oxo, phenyl, 2-pyridyl, 3-pyridyl, 4-pyridyl, 4-piperidinyl, 2-morpholinoethyl,

3-picolyl, arylalkyl (as defined above), -COOR_a, -CH₂COOR_a, -OCH₂COOR_a, -CONRbRc, -CH₂CQNR_bR_c (Q is =0 or =S), -OCH₂CONR_bR_c, -COO(CH₂)₂NR_eRf, -SO₂Ti, -CONR_dSO₂Ti, -NR_eR_f, -NR_gCHO, -NR_gCOT₂, -NR_gCOOT₂, -NRgCONRjRj, -NR_kSO₂T₃, -SO₂NR|R_m, -SO₂NR_nCOT₄, methylenedioxy and ethyleneoxy, wherein alkoxy has 1 to 8 carbon atoms, alkylthio has 1 to 8 carbon atoms, and acyloxy has 1 to 8 carbon atoms, and wherein R_a-R_n is hydrogen, alkyl (as defined above) or arylalkyl (as defined above), or

R_b and R_c, R_e and R_f, R, and Rj, and R| and R_m of -NR_bR_c, -NR_eR_f,

-NRjRj and -NR|R_m may be respectively bonded to each other to form heterocycles as defined above each optionally having 1 or 2 nitrogen atoms or oxygen atoms, and said heterocycle may be condensed with an aromatic ring as defined above optionally substituted by the substituents as defined above, or -NR_eRf may be a heteroaryl having =0, and

Ή - T₄ is selected from hydrogen atom and as defined above, alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl and heteroarylalkyl, and haloalkyl selected from trifluoromethyl, 2,2,2-trifluoroethyl and pentafluoroethyl, each of which may be substituted by the substituents as defined above.

(9) A compound of the formula (l-b)

9e

CA 02418656 2010-09-29

27103-383

<img file="CA2418656C_D0010.tif" />

wherein

Z is a hydrogen atom or a cyano, and

R³⁵ is -COR⁴¹ wherein R⁴¹ is a hydrogen atom, alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl or heteroarylalkyl, or -COOR⁴² wherein R⁴² is alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl or heteroarylalkyl, and of the above-mentioned groups, alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl and heteroarylalkyl each optionally have substituents.

(10) A compound of the formula (l-b), as shown above in the aforementioned (9), wherein Z is as defined in the aforementioned (6) and R³⁵ is as defined in the aforementioned (8).

The present invention further relates to the following compositions for pharmaceutical agents.

(11) A pharmaceutical composition containing an L-proline derivative of any of the aforementioned (1)-(6) or a pharmaceutically acceptable salt thereof, and a pharmacologically acceptable carrier.

(12) A DPP-IV inhibitor containing an L-proline derivative of any of the aforementioned (1)-(6) or a pharmaceutically acceptable salt thereof as an active ingredient.

(13) A therapeutic agent for the disease where DPP-IV is involved, which contains an L-proline derivative of any of the aforementioned (1)-(6) or a pharmaceutically acceptable salt thereof as an active ingredient.

9f

CA 02418656 2010-09-29

27103-383 (14) The therapeutic agent of the aforementioned (13), wherein the aforementioned disease is diabetes, obesity, HIV infection, cancer metastasis, dermopathy, prostatic hyperplasia, periodontitis or autoimmune disease.

The symbols used in the present specification are explained in the 5 following.

Alkyl is preferably linear or branched alkyl having 1 to 8 carbon atoms, such as methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tert-butyl, pentyl, hexyl, octyl and the like.

Acyl is preferably linear or branched and has 1 to 8

9g

CA 02418656 2003-02-06 carbon atoms, such as formyl, acetyl, propionyl, butyryl, isobutyryl, valeryl, isovaleryl, hexanoyl and the like.

Cycloalkyl preferably has 3 to 7 carbon atoms, and is exemplified by cyclopropyl, cyclopentyl, cyclohexyl, cycloheptyl and the like.

Cycloalkylalkyl has a cycloalkyl moiety, which is equivalent to that mentioned above, and an alkyl moiety which is preferably linear or branched and has 1 to 3 carbon atoms. Examples thereof include cyclopropylmethyl, 2-cyclobutylethyl, io 3-cyclopentylpropyl, cyclohexylmethyl, 2-cyclohexylethyl, cycloheptylmethyl and the like.

Aryl is preferably phenyl, naphthyl or ortho fused bicyclic group having 8 to 10 ring atoms wherein at least one ring is an aromatic ring (e.g., indeny1) and the like.

is Arylalkyl has an aryl moiety, which is equivalent to that mentioned above, and an alkyl moiety which is preferably linear or branched and has 1 to 3 carbon atoms. Examples thereof include benzyl, benzhydryl, phenethyl, 3-phenylpropyl, 1-naphthylmethyl, 2-(1-naphthyl)ethyl, 2-(2-naphthyl)ethyl, 320 (2-naphthyl)propyl and the like.

Arylalkenyl has an aryl moiety, which is equivalent to that mentioned above, and an alkenyl moiety which has 2 or 3 carbon atoms and is linear or branched. Examples thereof include styryl and the like.

Heteroaryl is preferably a 5 or 6-membered ring group having carbon and 1-4 hetero atoms (oxygen, sulfur or nitrogen), an ortho fused bicyclic heteroaryl having 8 to 10 ring atoms, which is derived therefrom, particularly a benz derivative, those derived from fusing a propenylene, trimethylene or so tetramethylene group therewith, stable N-oxide thereof and the like. Examples thereof include pyrrolyl, furyl, thienyl, oxazolyl, isoxazolyl, imidazolyl, thiazolyl, isothiazolyl, pyrazolyl, triazolyl, tetrazolyl, 1,3,5-oxadiazolyl, 1,2,410

CA 02418656 2003-02-06 oxadiazolyl, 1,3,4-oxadiazolyl, 1,2,4-thiadiazolyl, pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, 1,2,4-triazinyl, 1,2,3triazinyl, 1,3,5-triazinyl, benzoxazolyl, benzisoxazolyl, benzothiazolyl, benzisothiazolyl, benzimidazolyl, oxazolopyridyl, imidazopyridazinyl, thianaphthenyl, isothianaphthenyl, benzofuranyl, isobenzofuranyl, benzothienyl, chromenyl, isoindolyl, indolyl, indolinyl, indazolyl, isoquinolyl, quinolyl, phthalazinyl, quinoxalinyl, quinazolinyl, cinnolinyl, 2,1,3-benzoxadiazolyl, benzoxazinyl and the like.

Heteroarylalkyl has a heteroary1 moiety, which is equivalent to that mentioned above, and an alkyl moiety which_ is preferably has 1 to 3 carbon atoms and linear or branched. Examples thereof include 2-pyrrolylmethyl, 2-pyridylmethyl, 3pyridylmethyl, 4-pyridylmethyl, 2-thienylmethyl, 2-(2pyridyl)ethyl, 2-(3-pyridyl)ethyl, 2-(4-pyridyl)ethyl, 3-(2pyrrolyl)propyl, 4-imidazolylmethyl and the like.

Heterocycle is a saturated or unsaturated monocyclic 4 to 7-membered ring group or 10 or 11-membered ring group, which is a spiro ring, having carbon and at least one nitrogen, and optionally other hetero atom (oxygen or sulfur). Examples thereof include azetidinyl, pyrrolidinyl, piperidino, piperazinyl, morpholino, 1,4-diazepanyl, 1,2,5,6tetrahydropyridyl, thiomorpholino, oxothiomorpholino, dioxothiomorpholino, 3-azaspiro[5,5]undecyl, 1,3,8triazaspiro[4,5]decyl and the like.

Moreover, the above-mentioned heterocycle may be condensed with an aromatic ring (e.g., benzene ring, pyridine ring and the like) optionally having substituents. Examples thereof include indolinyl, isoindolinyl, 1,2,3,4tetrahydroquinolyl, 1,2,3,4-tetrahydroisoquinoly1, phthalimido, indolyl and the like.

Halogen is exemplified by chlorine, bromine, fluorine and iodine.

CA 02418656 2003-02-06

Haloalkyl is exemplified by trifluoromethyl, 2,2,2trifluoroethyl, pentafluoroethyl and the like.

Of the above-mentioned substituents, alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, arylalkenyl, heteroaryl, heteroarylalkyl and heterocycle are optionally substituted by the following 1 or more substituents.

The substituents may be, for example, halogen, hydroxyl group, nitro, cyano, trifluoromethyl, alkyl, alkoxy, alkylthio, formyl, acyloxy, oxo, phenyl, 2-pyridyl, 3-pyridyl, 4-pyridyl,

4-piperidinyl, 2-morpholinoethyl, 3-picolyl, arylalkyl, -COORa, -CHjCOORa, -OCHj COORa, -CONRtRc, -CH₂CQNRbRc (Q is =0 or =S) , -OCH₂ CONRb Re , -COO( CH₂ ) ₂ NReR_f, -SO₂ T_x, -CONRa SO₂ Ti, -NRe Rf, -NRgCHO, -NRgCOT₂ , -NRgCOOT₂ , -NRgCONRiRj , -NR_kSO₂T₃, SO₂NRiR_ro, -SO₂NR_nCOT₄, methylenedioxy, ethyleneoxy and the like.

These substituents may have substituents, and phenyl, 2pyridyl and 4-piperidinyl having substituents are exemplified by 4-cyanophenyl, 4-chlorophenyl, 4-methoxyphenyl, 5-cyano-2pyridyl, l-ethoxycarbonyl-4-piperidinyl and the like.

In the above-mentioned substituents of the substituents, halogen, alkyl and arylalkyl are exemplified by those mentioned above.

Alkoxy is linear or branched and preferably has 1 to 8 carbon atoms. Examples thereof include methoxy, ethoxy, propoxy, butoxy, pentyloxy, hexyloxy, octyloxy and the like. Alkylthio is linear or branched and preferably has 1 to 8 carbon atoms. Examples thereof include methylthio, ethylthio, propylthio, butylthio, pentylthio, hexylthio, octylthio and the like. Acyloxy is linear or branched and preferably has 1 to 8 carbon atoms. Examples thereof include formyloxy, acetyloxy, propionyloxy, butyryloxy, valeryloxy, pivaloyloxy, hexanoyloxy, benzoyloxy and the like.

Ra-R_n show hydrogen, alkyl (as defined above) or

CA 02418656 2003-02-06 arylalkyl (as defined above). Rt and Rc , Re and Rf, Ri and Rj , and Ri and Rm of -NRt&gt;Rc, -NR«Rf , -NRiRj and -NRiR^ may be respectively bonded to each other to form heterocycles each optionally having 1 or 2 nitrogen atoms or oxygen atoms, and the heterocycle may be condensed with an aromatic ring optionally having substituents (as defined above, and optionally substituted by the aforementioned substituents). Moreover, -NReRf may show a heteroaryl having =0 (e.g., 2pyrrolidinon-l-yl, succinimido, oxazolidin-2-on-3-yl, 2benzoxazolinon-3-yl, phthalimido, cis-hexahydrophthalimido and the like). Ti-T₄ show hydrogen atom, alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl or haloalkyl, which may be substituted by the aforementioned substituents.

Due to the asymmetric carbon, to which X is bonded, in compound (I), compound (I) can be present as an optically active compound or a diastereomer mixture, and the diastereomer mixture can be separated into each optically active compound by a known method.

The compound (I) can show polymorphism, and can be present as two or more tautomers.

Therefore, the present invention encompasses any of the above-mentioned stereoisomers, optical isomers, polymorphs, tautomers, optional mixtures thereof and the like.

The pharmaceutically acceptable salt of compound (I) includes, for example, inorganic acid addition salts (e.g., salts with hydrochloric acid, hydrobromic acid, hydriodic acid, sulfuric acid, nitric acid, phosphoric acid and the like), organic acid addition salts (e.g., salts with methanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid, formic acid, acetic acid, trifluoroacetic acid, oxalic acid, citric acid, malonic acid, fumaric acid, glutaric acid, adipic acid, maleic acid, tartaric acid, succinic acid, mandelic acid, malic acid,

CA 02418656 2003-02-06 pantothenic acid, methylsulfuric acid and the like), salts with amino acids (e.g., salts with glutamic acid, aspartic acid and the like), and the like.

The L-proline derivative of the present invention can be produced by the following method.

Scheme 1 shows a production method of a compound of the formula (I) wherein X is -OR¹⁰ or -OCOR¹¹.

Scheme 1

CA 02418656 2003-02-06

<img file="CA2418656C_D0011.tif" />

a

<img file="CA2418656C_D0012.tif" />

Step b

<img file="CA2418656C_D0013.tif" />

(XU') (XU)

<img file="CA2418656C_D0014.tif" />

Step e

<img file="CA2418656C_D0015.tif" />

(XIII)

OA'

<img file="CA2418656C_D0016.tif" />

(XIII') wherein R³⁵ is an amino acid-protecting group (e.g., tertbutoxycarbonyl (Boc) and benzyloxycarbonyl (Cbz)), R^{3 6} is a hydroxy1-protecting group (e.g., tert-butyldimethylmethylsilyl, triisopropylsilyl and tert-butyldiphenylsilyl), A' is R¹⁰ or COR¹¹ and other symbols are as defined above.

When Z of a compound of the formula (VIII) is cyano, this compound can be prepared by a method described in a reference [Ashworth et al., Bioorganic &amp; Medicainal Chemistry

CA 02418656 2003-02-06

Letters, vol. 6, 1163-1166 (1996)] or a conventional method based on such reference. A compound of the formula (IX') [hereinafter to be referred to as compound (IX')] can be prepared by silylation of hydroxyl group of hydroxyproline derivative by a conventional method.

Step a: Compound (VIII) is reacted with compound (IX) to give amide compound (XI), or compound (VIII) is reacted with compound (IX') to give amide compound (X).

As a condensation agent for activating carboxylic acid of compound (IX) or (IX'), there are recited, for example, dicyclohexylcarbodiimide (DCC), N-(3-dimethylaminopropyl)-N'ethylcarbodiimide (EDC), hydrochloride thereof, 2-ethoxy-lethoxycarbonyl-1,2-dihydroxyquinoline (EEDQ), carbodiimidazole (CDI), diethylphosphoryl cyanide, benzotriazol-1yloxytrispyrrolidinophosphonium hexafluorophosphate (PyBOP), diphenylphosphoryl azide (DPPA), isobutyl chloroformate, diethylacetyl chloride, trimethylacetyl chloride and the like. These condensation agent may be used alone, or an additive such as N-hydroxysuccinimide (HONSu), hydroxybenzotriazole (HOBT) or

3-hydroxy-4-oxo-3,4-dihydro-l,2,3-benzotriazine (HOOBT), 4dimethylaminopyridine (DMAP) and the like is used in combination.

This reaction is generally carried out in an inert solvent and the inert solvent to be used may be any as long as it is aprotic. Preferable examples include acetonitrile, tetrahydrofuran, dichloromethane, chloroform, N,Ndimethylformamide (DMF) and the like. This condensation is carried out generally at a temperature of from -30°C to 80°C, preferably from -10°C to 25°C.

Step b: Compound (X) is deprotected to give compound (XI').

This reaction can be carried out in an inert solvent such as tetrahydrofuran and the like, using tetrabutylammonium fluoride and the like. The reaction mixture is preferably

CA 02418656 2003-02-06 buffered with an acid such as acetic acid and the like generally at a temperature of from -30°C to 70°C for 10 min to hr.

Step c: Compound (XI) is reacted to give compound (XII), or compound (XI') is reacted to give compound (XII').

By acylation of hydroxyl group of compound (XI) or (XI') by a general method, a compound of the formula (XII) or (XII'), wherein A' is COR¹¹, can be obtained. The synthetic method of compound (XII) or (XII'), wherein R¹¹ is alkyl, cycloalkyl, io cycloalkylalkyl, aryl, arylalkyl, heteroaryl or heteroarylalkyl, is exemplified by a method including use of an active carboxylic acid derivative such as acid halide, a method including use of a carboxylic acid and a coupling agent, and the like.

is A compound of the formula (XII) or (XII') wherein A' is

R¹⁰ can be obtained by converting hydroxyl group of compound (XI) or (XI') to alcoholate, which is followed by nucleophilic substitution of alkyl halide and the like. The reaction is carried out in the presence of a base such as sodium hydride and the like, using alkyl halide and the like in an inert solvent such as tetrahydrofuran and the like, generally at a temperature of from -80°C to 60°C, preferably from -50°C to 25°C. Step d: Compound (XI) is reacted to give compound (XII'), or compound (XI') is reacted to give compound (XII).

The reaction is carried out in the presence of phosphines such as triphenylphosphine, tributylphosphine and the like and diazodicarboxylic acid diester, using R^X1COOH or aryl or heteroaryl having a hydroxyl group, and the like in an inert solvent such as toluene, tetrahydrofuran and the like, generally at a temperature of from -30°C to 110°C, preferably from 0°C to 25°C.

A compound of the formula (XII') or (XII), wherein A' is COR¹¹, can be obtained by sulfonylation of hydroxyl group of

CA 02418656 2003-02-06 compound (XI) or (XI'), and reacting the compound with carboxylic acid salt. The sulfonylation is carried out in the presence of a base such as pyridine, triethylamine and the like using p-toluenesulfonyl chloride, methanesulfonyl chloride, trifluoromethanesulfonyl chloride and the like, in an inert solvent such as dichloromethane, 1,2-dichloroethane and the like generally from -30°C to 60°C for 10 min to 24 hr. The subsequent reaction with carboxylic acid salt is carried out in the presence of an inorganic base such as potassium carbonate, sodium carbonate and the like using R¹¹COOH in an inert solvent such as acetone, hexamethylphosphoramide and the like generally from 0°C to 120°C for 10 min to 24 hr.

Step e: Compound (XII) is deprotected to give compound (XIII), or compound (XII') is deprotected to give compound (XIII').

When, in this reaction, the protecting group is Boc group, for example, the compound is reacted in a solvent such as acetonitrile, tetrahydrofuran, 1,4-dioxane, ethyl acetate, methanol, ethanol, chloroform and the like, using an acid such as hydrogen chloride, trifluoroacetic acid and the like generally from -30°C to 60°C for 10 min to 24 hr for deprotection.

Scheme 2 shows a production method of a compound of the formula ( I ) wherein X is -NR¹ R² .

Scheme 2

CA 02418656 2003-02-06

<img file="CA2418656C_D0017.tif" />

<img file="CA2418656C_D0018.tif" />

<img file="CA2418656C_D0019.tif" />

<img file="CA2418656C_D0020.tif" />

Step k

Ο (XXII) wherein OSO₂R³⁷ is a leaving group (e.g., tosylate (OTs), mesylate (OMs) or triflate (OTf)), Hal is halogen, R³⁸ and R³⁹are the same or different and each is independently hydrogen atom, alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heteroaryl or heteroarylalkyl, or may be bonded to each other to form a heterocycle optionally containing 1 or 2 nitrogen atoms or oxygen atoms, or the heterocycle may be condensed with an aromatic ring optionally having substituents, and other symbols are as defined above.

Step f: Hydroxyl group of compound (XI) is sulfonylated to give compound (XIV).

This reaction is carried out in the presence of a base such as pyridine, triethylamine and the like, using p15 toluenesulfonyl chloride, methanesulfonyl chloride, trifluoromethanesulfonyl chloride and the like in an inert solvent such as dichloromethane, 1,2-dichloroethane and the like generally at a temperature of from -30°C to 60°C for 10

CA 02418656 2003-02-06 min to 24 hr.

Step g: Compound (XIV) is subjected to azidation to give compound (XV).

The reaction is carried out using a metal azide, such as sodium azide, in a solvent such as Ν,Ν-dimethylformamide and the like generally at a temperature of from 0°C to 120°C for 30 min to 24 hr.

Step h: Compound (XV) is directly obtained from compound (XI).

The reaction is carried out in the presence of phosphines such as triphenylphosphine, tributylphosphine and the like, and diazodicarboxylic acid diester using hydrogen azide, DPPA, zinc azide bispyridine complex salt and the like in an inert solvent such as toluene, tetrahydrofuran and the like generally at a reaction temperature of from -30°C to 100°C. Step i: Compound (XV) is reduced to give compound (XVI).

For this reaction, catalytic reduction using palladium, platinum, nickel and the like, reduction by metalhydride, reduction by triphenylphosphine, thiol, sulfide, diborane, or transition metal and the like are mentioned.

Step j: Compound (XVI) is reacted with compound (XVII) or (XVII') to give compound (XIX).

The reaction is carried out in the presence of a base such as triethylamine, diisopropylethylamine and the like in an inert solvent such as N-methyl-2-pyrrolidone, Ν,Νdimethylformamide, tetrahydrofuran and the like at a temperature of from 0°C to near boiling point of solvent, preferably from 0°C to 80°C.

Step k: Compound (XVI) is reacted with compound (XVIII), which is followed by reduction to give compound (XIX).

This reaction is carried out in the presence of sodium borohydride, sodium cyanoborohydride, sodium triacetoxyborohydride and the like in an inert solvent such as methanol, ethanol, dichloromethane, 1,2-dichloroethane,

CA 02418656 2003-02-06 tetrahydrofuran, acetonitrile, 1,4-dioxane and the like, and using, where necessary, an acid catalyst such as acetic acid, p-toluenesulfonic acid, boron trifluoride diethylether complex and the like, generally at a temperature of from 0°C to 100°C for 10 min to 20 hr.

By repeating Step j or k as necessary, compound (XIX) wherein R¹ and R² may be the same or different can be obtained. Step 1: Compound (XIX) is directly obtained from compound (XI).

The reaction is carried out in the presence of phosphines such as triphenylphosphine, tributylphosphine and the like and diazodicarboxylic acid diester using iminodicarbonates, sulfonamides, a nitrogen-containing compound having N-H hydrogen and having high acidity, and the like in an inert solvent such as toluene, tetrahydrofuran and the like. Step m: Compound (XI) is oxidized to give (XX).

This reaction is carried out by, for example, a method using pyridine sulfur trioxide complex and dimethyl sulfoxide at room temperature. Examples of useful other methods include a method using alkaline potassium permanganate solution; a method using oxalyl chloride, dimethyl sulfoxide and tertiary amine; a method using acetic anhydride and dimethyl sulfoxide; a method using EDC and dimethyl sulfoxide with dichloroacetic acid as a catalyst; a method using chromium oxide (XI) pyridine complex in dichloromethane; a method using aqueous sodium hypochlorite solution with TEMPO free radical as a catalyst in the presence of sodium bromide in ethyl acetate and toluene, and the like.

Step n: Compound (XX) is reacted with compound (XXI), which is followed by reduction to give compound (XIX).

This reaction is carried out in the presence of sodium borohydride, sodium cyanoborohydride, sodium triacetoxyborohydride and the like, in an inert solvent such as methanol, ethanol, dichloromethane, 1,2-dichloroethane,

CA 02418656 2003-02-06 tetrahydrofuran, acetonitrile, 1,4-dioxane and the like, and using as necessary an acidic catalyst, such as acetic acid, ptoluenesulfonic acid, boron trifluoride diethyl ether complex and the like generally at a temperature of from 0°C to 100°C for 10 min to 20 hr.

The compound (XXI) can be synthesized by a known method. Step o is the same as Step e of Scheme 1.

The production method of a compound of the formula (I) wherein X is -NR³COR⁴ is shown in Scheme 3.

Scheme 3

<img file="CA2418656C_D0021.tif" />

(XXlll)

<img file="CA2418656C_D0022.tif" />

step q

<img file="CA2418656C_D0023.tif" />

(XXV) wherein each symbol in the formula is as defined above.

The compound (XXIII) is the same as compound (XVI) obtained in Scheme 2 or a compound (XIX) wherein one of R¹ and R² is hydrogen atom.

Step p: Compound (XXIII) is reacted to give amide compound (XXIV).

The reaction is carried out using R⁴COC1 or R⁴CO₂H after conversion to an acid halide with thionyl chloride, thionyl bromide and the like or conversion to a mixed acid anhydride with pivaloyl chloride, isobutyl chloroformate and the like, in the presence of a tertiary base such as triethylamine, pyridine, Ν,Ν-dimethylaniline and the like, in an inert solvent such as toluene, hexane, tetrahydrofuran, 1,4-dioxane, ethyl acetate, chloroform, Ν,Ν-dimethylformamide, dimethyl sulfoxide, hexamethylphosphoramide and the like, generally from 0°C to 120°C for 10 min to 10 hr.

For the reaction with R⁴CO₂H, a condensation agent shown

CA 02418656 2003-02-06 in Step a can be an inert solvent also such dichloromethane, used and the reaction is carried out in as acetonitrile, tetrahydrofuran, chloroform, Ν,Ν-dimethylformamide and the like generally at a temperature of from -30°C to 80°C, from -10°C to 25°C.

preferably

Step q is the same as The production method wherein X is -NR^SCONR⁶R⁷

Step e of Scheme 1.

of a compound of the formula (I) is shown in Scheme 4.

Scheme 4

<img file="CA2418656C_D0024.tif" />

defined (XXVIII) above.

(XXVII) wherein each symbol in the formula is as

The compound (XXVI) is the same as obtained in Scheme 2 or a compound (XIX) R² is hydrogen atom.

Step r: Compound (XXVI) is reacted to give urea compound compound (XVI) wherein one of R¹ and (XXVII).

The reaction is carried out using isocyanate such as R⁶NCO and the like in an inert solvent such as toluene, chloroform, dichloromethane, tetrahydrofuran and the like generally at a temperature of from -20°C to 80°C, preferably from 0°C to 25°C.

Alternatively, there is a method using, for example, carbodiimidazole, phosgene, diphosgene (trichloromethyl chloroformate), triphosgene [bis(trichloromethyl)carbonate] and the like, together with an amine represented by R⁶R⁷NH and a tertiary base such as triethylamine and the like.

Step s is the same as Step e of Scheme 1.

The production method of a compound of the formula (I)

CA 02418656 2003-06-03

27103-383 wherein X is -NR⁸ SO₂ R⁹ is shown in Scheme 5.

Scheme 5

<img file="CA2418656C_D0025.tif" />

(XXIX) step u (XXX) rL,so₂r⁹

N &lt;5 A

HN—χ,Ν—

Y ^z (XXXI) wherein each symbol in the formula is as defined above.

The compound (XXIX) is the same as compound (XVI) obtained in Scheme 2 or a compound (XIX) wherein one of R¹ and R² is hydrogen atom.

Step t: Compound (XXIX) is reacted to give sulfonamide compound (XXX).

The reaction is carried out using sulfonyl chloride such as R⁹-SO₂C1 and the like in the presence of an organic base such as triethylamine, pyridine and the like or an inorganic base such as sodium carbonate, potassium carbonate, sodium hydroxide and the like, in a solvent such as water, toluene, chloroform, dichloromethane, tetrahydrofuran and the like generally at a temperature of from -20°C to 80°C.

Alternatively, there is a method wherein compound (XXIX) and sulfuryl chloride are reacted in the presence of a tertiary

2o base such as triethylamine and the like in an inert solvent such as chloroform, dichloromethane, tetrahydrofuran and the like to give sulfamyl chloride, which is reacted with aryl compound in the presence of a Lewis acid such as aluminum chloride and the like.

Step u is the same as Step e of Scheme 1.

A different production method of compound (XXII) is shown in Scheme 6. This production method is useful when both R¹ and R² are not hydrogen atoms.

CA 02418656 2003-02-06

<img file="CA2418656C_D0026.tif" />

Scheme 6

<img file="CA2418656C_D0027.tif" />

<img file="CA2418656C_D0028.tif" />

<img file="CA2418656C_D0029.tif" />

Step x

NR¹R²

<img file="CA2418656C_D0030.tif" />

O (XIX) wherein R⁴⁰ is alkyl such as methyl, ethyl and the like, benzyl and the like, and other symbols are as defined above.

Step v is the same as the method for conversion of compound (XI) to compound (XIX) shown in Scheme 2.

Step w: Ester-protected carboxyl group of compound (XXXIII) is deprotected to give compound (XXXIV).

io For reaction, general deprotection can be used. For example, sodium hydroxide, potassium hydroxide, sodium carbonate, potassium carbonate and the like are hydrolyzed under alkali conditions, or when R⁴⁰ is benzyl, catalytic hydrogenation and the like are conducted in the presence of platinum, palladium and the like, in an inert solvent such as methanol, ethanol and the like for deprotection.

Step x: Compound (VIII) and compound (XXXIV) are reacted to give amide compound (XIX)·

The reaction is carried out using a condensation agent shown in Step a, in an inert solvent such as acetonitrile, tetrahydrofuran, dichloromethane, chloroform, N,Ndimethylformamide and the like generally at a temperature of

CA 02418656 2003-02-06 from -30°C to 80°C, preferably from -10°C to 25°C.

The compound of the formula (XXIV) shown in Scheme 3, the compound of the formula (XXVII) shown in Scheme 4 and the compound of the formula (XXX) shown in Scheme 5 can be produced by the routes shown in Scheme 6.

The production method of compound (XXII), wherein the asymmetric carbon, to which NR^XR² is bonded, is shown by S configuration, is shown in Scheme 2 using the compound of the formula (XI) as a starting material, and in Scheme 6, using the compound of the formula (XXXII) as a starting material.

Besides the above-mentioned, a compound (XXII'), wherein the asymmetric carbon, to which NR^XR² is bonded, is shown by R configuration,

<img file="CA2418656C_D0031.tif" />

wherein each symbol in the formula is as defined above, can be also produced by a method similar to the one mentioned above using compound (XI')

<img file="CA2418656C_D0032.tif" />

(XI¹) wherein each symbol in the formula is as defined above, or a compou nd (XXXII')

<img file="CA2418656C_D0033.tif" />

(XXXII·) wherein each symbol in the formula is as defined above, as a

CA 02418656 2003-02-06 starting material.

When Z in each formula shows cyano, each compound up to an intermediate is produced with Z of the formula as a carbamoyl group and dehydrated by a known method for conversion thereof to cyano group.

This reaction is carried out using diphosphorus pentaoxide, phosphorus oxychloride-imidazole, trifluoroacetic acid anhydride, p-toluenesulfonyl chloride-pyridine and the like as a dehydrating agent in an inert solvent such as dichloromethane, pyridine and the like.

The L-proline derivative of the formula (I) of the present invention produced in this manner can be obtained at an optional purity by applying a known separation and purification means as necessary, such as concentration, extraction, chromatography, reprecipitation, recrystallization and the like.

The L-proline derivative of the formula (I) can be prepared into an acid addition salt as necessary with an inorganic acid such as hydrochloric acid, hydrobromic acid, hydriodic acid, sulfuric acid, nitric acid, phosphoric acid and the like or an organic acid such as methanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid, formic acid, acetic acid, trifluoroacetic acid, oxalic acid, citric acid, malonic acid, fumaric acid, glutaric acid, adipic acid, maleic acid, tartaric acid, succinic acid, mandelic acid, malic acid, pantothenic acid, methylsulfuric acid and the like. In addition, it is also present as a solvate such as hydrate and the like.

The compound of the formula (I) or a pharmacologically acceptable salt of the present invention has a superior DPP-IV inhibitory activity in mammals (e.g., human, dog, cat, rat and the like).

Therefore, the compound of the present invention (I) or a pharmacologically acceptable salt thereof is useful as a DPP27

CA 02418656 2003-02-06

IV inhibitor and useful for the prophylaxis or treatment of various diseases considered to be caused by DPP-IV, for example, for the prophylaxis or treatment of diseases where GLP-1 is considered to be involved (e.g., diabetes, obesity and the like), and the like.

The compound (I) of the present invention can be administered to a same subject with other therapeutic drug for diabetes, therapeutic drug for diabetic complications, antihyperlipidemic agent, antihypertensive agent and the like at the same time or timelag. As used herein, examples of the therapeutic drug for diabetes include insulin sensitivity increasing agent, α-glucosidase inhibitor, biguanide agent and the like. Examples of the therapeutic drug for diabetic complications include aldose reductase inhibitor. As the antihyperlipidemic agent, statin compound, which is a cholesterol synthetase inhibitor, squalene synthetase inhibitor, fibrates having triglyceride lowering effect, and the like can be mentioned. As the antihypertensive agent, calcium antagonist, angiotensin converting enzyme inhibitor, angiotensin II antagonist and the like can be mentioned. When the compound of the present invention is used on combination with multiple agents, the mixing ratio thereof can be appropriately determined depending on the subject of administration, age and body weight of the administration subject, symptom, administration time, dosage form, administration method, combination and the like.

When the compound (I) of the present invention or an acid addition salt thereof is used as the aforementioned pharmaceutical agent, it is used on its own or admixed with an appropriate pharmacologically acceptable carrier, an excipient, a diluent and the like in the form of powder, granule, tablet, capsule, injection and the like, and administered orally or parenterally. The above-mentioned preparation contains an

CA 02418656 2003-02-06 effective amount of compound (I) or a pharmacologically acceptable salt thereof.

While the dose of the compound (I) or a pharmacologically acceptable salt thereof varies depending on the administration route, target disease, symptom, body weight and age of patients, and the compound to be used, it can be determined as appropriately according to the administration object. Generally, when orally administered to an adult, 0.011000 mg/kg body weight/day, preferably 0.05-500 mg/kg body weight/day, is preferably administered once a day or in several doses a day.

Examples

The present invention is explained in detail by referring to Reference Examples and Examples, which are not to be construed as limitative.

¹ H-NMR was measured at 300 MHz unless particularly indicated. The chemical shift of ¹ H-NMR relative delta (δ) value was expressed in parts per million (ppm) using tetramethylsilane (TMS) as the internal standard. For the coupling constant, obvious multiplicity is shown in hertz (Hz) using s (singlet), d (doublet), t (triplet), q (quartet), quint (quintet), m (multiplet), dd (doublet of doublets), td (triplet of doublets), brs (broad singlet) and the like. Thin-layer chromatography used was manufactured by Merck, and column chromatography was performed using silica gel manufactured by Fuji silysia chemical.

For drying organic solutions in extraction, anhydrous sodium sulfate or anhydrous magnesium sulfate was used, unless particularly indicated.

Reference Example 1

Synthesis of (S)-l-((2S,4S)-l-tert-butoxycarbonyl-4-hydroxy-2pyrrolidinylcarbonyl)-2-cyanopyrrolidine (1) N-tert-Butoxycarbonyl-L-cis-4-hydroxyproline (23.1 g) and

CA 02418656 2003-02-06 imidazole (30.0 g) were dissolved in DMF (300 mL). tertButyldimethylsilyl chloride (33.3 g) was added thereto. After stirring at room temperature for 16 hr, water (300 mL) was gradually added under ice-cooling. The reaction solution was acidified with 10% aqueous citric acid solution and extracted with ethyl acetate. The extract was washed 3 times with water and with brine, and dried. The solvent was distilled away under reduced pressure. The residue was purified by silica gel chromatography to give N-tert-butoxycarbonyl-L-cis-4-tertbutyldimethylsilyloxyproline (27.4 g) as a white solid.

(2) The above-mentioned compound (27.4 g) and (S)-2cyanopyrrolidine hydrochloride (10.4 g) were dissolved in DMF (250 mL), and triethylamine (10.9 mL), HOBT (14.3 g) and EDC hydrochloride (18.0 g) were added successively. The mixture was stirred at room temperature for 15 hr. The reaction mixture was added to saturated aqueous sodium hydrogencarbonate solution, and extracted with ethyl acetate. The extract was washed with water and brine, and dried. The solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-l-[(2S,4S)-l-tertbutoxycarbonyl-4-tert-butyldimethylsilyloxy-2pyrrolidinylcarbonyl]-2-cyanopyrrolidine (25.3 g) as a slightly yellow oil.

(3) The above-mentioned compound (25.3 g) was dissolved in tetrahydrofuran (300 mL), and a 1.0 mol/L solution (60 mL) of tetrabutylammonium fluoride in tetrahydrofuran was added dropwise under ice-cooling. The mixture was stirred at room temperature for 3 hr, and the solvent was evaporated under reduced pressure. The residue was added to brine, and extracted with ethyl acetate. The extract was dried, and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give the title compound (20.6 g) as a white solid.

CA 02418656 2003-02-06 ¹ H-NMR(DMSO-de)01.25-1.45(9H,m), 1.50-1.64(lH,m), 1.952.30(4H,m), 2.98-3.13(lH,m), 3.30-3.67(4H,m), 4.13-4.26(lH,m),

4.30-4.42(lH,m), 4.77-4.88(lH,m), 5.16(lH,d,J=6.5Hz).

Reference Example 2

Synthesis of (S)-l-((2S,4R)-l-tert-butoxycarbonyl-4-hydroxy-2pyrrolidinylcarbonyl)-2-cyanopyrrolidine

N-tert-Butoxycarbonyl-L-trans-4-hydroxyproline (68.4 g) and (S)-2-cyanopyrrolidine hydrochloride (39.2 g) were dissolved in DMF (350 mL), and triethylamine (41.4 mL), HOBT (49.9 g), and EDC hydrochloride (62.5 g) were successively added. The mixture was stirred at room temperature for 15 hr. The reaction mixture was concentrated and brine and saturated aqueous sodium hydrogencarbonate solution were added to the residue. The precipitated crystals were collected by filtration. The filtrate was extracted with ethyl acetate and the extract was dried. The solvent was evaporated under reduced pressure. The residue and the crystals were combined and washed with ethyl acetate to give the title compound (60.7 g) as a white solid.

¹ H-NMR(CDC1₃ )01.30-1.45(9H,m), 1.54-2.38(5H,m), 3.30-3.93(5H,m),

4.49-4.89(3H,m).

Reference Example 3

Synthesis of (S)-l-((2S,4S)-4-amino-l-tert-butoxycarbonyl-2pyrrolidinylcarbonyl)-2-cyanopyrrolidine (1) (S)-1-((2S,4R)-l-tert-Butoxycarbonyl-4-hydroxy-2pyrrolidinylcarbonyl)-2-cyanopyrrolidine (title compound of Reference Example 2, 60.7 g) and triethylamine (30.1 mL) were dissolved in DMF (300 mL). Methanesulfonyl chloride (16 mL) was added thereto under ice-cooling. After stirring at room temperature for 3 hr, the reaction mixture was washed with water and dried. The solvent was evaporated under reduced pressure. Ethanol was added to the residue to give (S)—1— ((2S,4R)-l-tert-butoxycarbonyl-4-methanesulfonyloxy-231

CA 02418656 2003-02-06 pyrrolidinylcarbonyl)-2-cyanopyrrolidine (64.1 g) as a white solid.

(2) The above-mentioned compound (64.1 g) and sodium azide (11.8 g) were dissolved in DMF (250 mL), and the mixture was stirred at 85°C for 5 hr. The reaction mixture was added to water and the precipitated crystals were collected by filtration to give (S)-l-((2S,4S)-4-azido-l-tertbutoxycarbonyl-2-pyrrolidinylcarbonyl)-2-cyanopyrrolidine (50.1 g) as a white solid.

(3) The above-mentioned compound (50.1 g) was dissolved in ethanol (500 mL). The mixture was stirred under a hydrogen atomosphere (1 atm) in the presence of 5% palladium/barium sulfate (5.9 g). The reaction mixture was filtrated and the filtrate was concentrated under reduced pressure. Diethyl ether was added thereto and the precipitated solid was collected by filtration to give the title compound (45.5 g) as a white solid.

^XH-NMR(CDC1₃)01.23-1.52(10H,m), 1.60-2.47(8H,m), 2.863.00(lH,m), 3.43-3.65(3H,m), 4.25-4.40(lH,m), 4.75-5.02(lH,m). Reference Example 4

Synthesis of (2S,4R)-l-tert-butoxycarbonyl-4-(5-cyano-2pyridyl)aminopyrrolidine-2-carboxylic acid (1) N-tert-Butoxycarbonyl-L-cis-4-hydroxyproline (24.5 g) and triethylamine (15.4 mL) were dissolved in dichloromethane (500 mL). Methanesulfonyl chloride (8.1 mL) was added thereto under ice-cooling. After stirring at room temperature for 3 hr, the reaction mixture was washed with water and dried. The solvent was evaporated under reduced pressure. The residue was dissolved in DMF (250 mL) and sodium azide (7.15 g) was added. The mixture was stirred at 80°C for 3 hr, added to water and extracted with ethyl acetate. The extract was dried, and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give methyl (2S,4R)-432

CA 02418656 2003-02-06 azido-l-tert-butoxycarbonylpyrrolidine-2-carboxylate (18.4 g) as an oil.

(2) The above-mentioned compound (18.3 g) was dissolved in methanol (200 mL), and the mixture was stirred under a hydrogen atomosphere (1 atm) in the presence of 5% palladium/carbon (5.9 g). The reaction mixture was filtrated and the filtrate was concentrated under reduced pressure to give methyl (2S,4R)-4amino-l-tert-butoxycarbonylpyrrolidine-2-carboxylate (16.6 g) as an oil.

(3) The above-mentioned compound (3.32 g) and triethylamine (1.4 mL) were dissolved in tetrahydrofuran (20 mL), and 2chloro-5-cyanopyridine (1.0 g) was added thereto. The mixture was stirred at 80°C for 4 hr. The reaction mixture was added to water and extracted with ethyl acetate. The extract was washed with saturated aqueous sodium hydrogencarbonate solution and brine, and dried. The solvent was evaporated under reduced pressure, and the residue was purified by silica gel chromatography to give methyl (2S,4R)-l-tert-butoxycarbonyl-4(5-cyano-2-pyridylamino)pyrrolidine-2-carboxylate (1.2 g) as a white solid.

(4) The above-mentioned compound (0.94 g) was dissolved in methanol (30 mL), and 1 mol/L aqueous sodium hydroxide solution (3.0 mL) was added thereto. The mixture was stirred at room temperature for 15 hr. The reaction mixture was concentrated and 5% aqueous citric acid solution was added to the residue. The mixture was extracted with ethyl acetate, and the extract was washed with brine and dried. The solvent was evaporated under reduced pressure to give the title compound (0.75 g) as amorphous.

¹H-NMR(CDC1₃)01.33-1.57(9H,m), 2.14-2.68(2H,m), 3.27-3.55(lH,m),

3.82-4.00(lH,m), 4.33-4.57(2H,m), 6.23(lH,brs),

6.50(lH,d,J=8.7Hz), 7.64(lH,dd,J=8.7,2.1Hz)8.33(lH,d,J=2.1Hz).

Reference Example 5

CA 02418656 2003-02-06

Synthesis of (2S,4S)-4-(2-benzoxazolyl)amino-l-tertbutoxycarbonylpyrrolidine-2-carboxylic acid (1) N-tert-Butoxycarbonyl-L-trans-4-hydroxyproline (20.7 g) and triethylamine (15.4 mL) were dissolved in dichloromethane (400 mL). p-Toluenesulfonic acid chloride (16.8 g) was added thereto under ice-cooling. After stirring at room temperature for 15 hr, the reaction mixture was washed with water and dried. The solvent was evaporated under reduced pressure and the residue was purified by silica gel chromatography to give methyl (2S,4R)-l-tert-butoxycarbonyl-4-(ptoluenesulfonyloxy)pyrrolidine-2-carboxylate (11.7 g).

(2) The above-mentioned compound (11.7 g) was dissolved in DMF (100 mL), and sodium azide (1.95 g) was added thereto. The mixture was stirred at 80°c for 3 hr. The reaction mixture was added to water, and extracted with ethyl acetate. The extract was dried, and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give methyl (2S,4S)-4-azide-l-tertbutoxycarbonylpyrrolidine-2-carboxylate (7.99 g) as an oil.

(3) The above-mentioned compound (7.99 g) was dissolved in methanol (150 mL). The mixture was stirred under a hydrogen atomosphere (1 atm) in the presence of 10% palladium/carbon (2 g). The reaction mixture was filtrated and the filtrate was concentrated under reduced pressure to give methyl (2S,4S)-4amino-1-tert-butoxycarbonylpyrrolidine-2-carboxylate (7.23 g) as an oil.

(4) The above-mentioned compound (2.4 g) and triethylamine (1.4 mL) were dissolved in tetrahydrofuran (20 mL), and 2chlorobenzoxazole (0.82 mL) was added thereto. The mixture was stirred at 60°C for 3 hr. The reaction mixture was added to water, and extracted with ethyl acetate. The extract was washed with saturated aqueous sodium hydrogencarbonate solution and dried. The solvent was evaporated under reduced pressure

CA 02418656 2003-02-06 and the residue was purified by silica gel chromatography to give methyl (2S,4S)-4-(2-benzoxazolyl)amino-l-tertbutoxycarbonylpyrrolidine-2-carboxylate (1.49 g) as amorphous.

(5) The above-mentioned compound (1.49 g) was dissolved in methanol (50 mL), and 1 mol/L aqueous sodium hydroxide solution (5.0 mL) was added thereto. The mixture was stirred for 15 hr and concentrated. The residue was extracted with water and the extract was washed with ethyl acetate. 5% Aqueous citric acid solution was added thereto to give the title compound (1.39 g) as a white solid.

¹H-NMR(DMSO-de)01.28-1.50(9H,m), 1.93-2.08(lH,m), 2.532.75(lH,m), 3.18-3.30(lH,m), 3.77-4.33(2H,m), 6.97-7.40(4H,m), 8.06-8.16(lH,m), 12.63(1H,brs).

Reference Example 6

Synthesis of (2S,4R)-4-(2-benzoxazolyl)amino-l-tertbutoxycarbonylpyrrolidine-2-carboxylic acid (1) Methyl (2S,4R)-4-amino-l-tert-butoxycarbonylpyrrolidine-2carboxylate [product of Reference Example 4(2), 3.32 g] and triethylamine (1.4 mL) were dissolved in tetrahydrofuran (20 mL). 2-Chlorobenzoxazole (0.86 mL) was added thereto and the mixture was stirred at room temperature for 48 hr. The reaction mixture was added to water and extracted with ethyl acetate. The extract was washed with saturated aqueous sodium hydrogencarbonate solution and brine, and dried. The solvent was evaporated under reduced pressure and the residue was purified by silica gel chromatography to give methyl (2S,4R)-4(2-benzoxazolyl) amino-1-tert-butoxycarbonylpyrrolidine-2carboxylate (1.48 g) as a white solid.

(2) The above-mentioned compound (1.31 g) was dissolved in methanol (30 mL) and 1 mol/L aqueous sodium hydroxide solution (4.4 mL) was added thereto. The mixture was stirred at room temperature for 15 hr and the reaction mixture was concentrated. To the residue was added 5% aqueous citric acid solution and

CA 02418656 2003-02-06 the mixture was extracted with ethyl acetate. The extract was washed with brine and dried. The solvent was evaporated under reduced pressure to give the title compound (1.11 g) as a white solid.

¹H-NMR(CDC1₃)61.32-1.68(9H,m), 2.20-2.80(2H,m), 3.37-3.62(lH,m),

3.88-4.10(lH,m), 4.37-4.68(2H,m), 7.01-7.43(4H,m).

Reference Example 7

Synthesis of (2S,4S)-4-benzoylamino-l-tertbutoxycarbonylpyrrolidine-2-carboxylic ac id (1) Methyl (2S,4S)-4-amino-l-tert-butoxycarbonylpyrrolidine-2carboxylate [product of Reference Example 5(3), 2.4 g] and triethylamine (2.0 mL) were dissolved in tetrahydrofuran (20 mL), and benzoyl chloride (1.1 mL) was added thereto. The mixture was stirred for 1 hr. The reaction mixture was added to water and extracted with ethyl acetate. The extract was washed with saturated aqueous sodium hydrogencarbonate solution and dried. The solvent was evaporated under reduced pressure and the residue was purified by silica gel chromatography to give methyl (2S,4S)-4-benzoylamino-l-tertbutoxycarbonylpyrrolidine-2-carboxylate (1.63 g) as a white solid.

(2) The above-mentioned compound (1.5 g) was dissolved in methanol (50 mL) and 1 mol/L aqueous sodium hydroxide solution (4.7 mL) was added thereto. The mixture was stirred for 15 hr. The reaction mixture was concentrated and 5% aqueous citric acid solution was added to the residue. The mixture was extracted with ethyl acetate, and the extract was washed with brine and dried. The solvent was evaporated under reduced pressure to give the title compound (1.1 g) as amorphous.

Reference Example 8

Synthesis of (2S,4R)-4-benzoylamino-l-tertbutoxycarbonylpyrrolidine-2-carboxylic acid (1) The product (3.32 g) of Reference Example 4(2) and

CA 02418656 2003-02-06 triethylamine (1.4 mL) were dissolved in tetrahydrofuran (20 mL), and benzoyl chloride (0.87 mL) was added thereto. The mixture was stirred for 1 hr. The reaction mixture was added to water and extracted with ethyl acetate. The extract was washed with saturated aqueous sodium hydrogencarbonate solution and dried. The solvent was evaporated under reduced pressure and the residue was purified by silica gel chromatography to give methyl (2S,4R)-4-benzoylamino-l-tertbutoxycarbonylpyrrolidine-2-carboxylate (2.4 g) as amorphous.

(2) The above-mentioned compound (2.4 g) was dissolved in methanol (80 mL), and 1 mol/L aqueous sodium hydroxide solution (8.2 mL) was added thereto. The mixture was stirred for 15 hr. The reaction mixture was concentrated and 5% aqueous citric acid solution was added to the residue. The mixture was extracted with ethyl acetate, and the extract was washed with brine and dried. The solvent was evaporated under reduced pressure to give the title compound (1.9 g) as a white solid. ¹H-NMR(CDC1₃)01.33-1.55(9H,m), 2.12-2.75(2H,m), 3.31-3.60(lH,m),

3.84-4.00(lH,m), 4.30-4.81(2H,m), 6.16-6.55(4H,m), 7.387.80(5H,m).

Reference Example 9

Synthesis of 3-[(2S,4R)-l-tert-butoxycarbonyl-4-hydroxy-2pyrrolidinylcarbonyl]-1,3-thiazolidine

N-tert-Butoxycarbonyl-L-trans-4-hydroxyproline (69.4 g) and thiazolidine (29.4 g) were dissolved in DMF (300 mL), and HOBT (50.5 g) and EDC hydrochloride (63.3 g) were successively added thereto. The mixture was stirred at room temperature for 18 hr and the reaction mixture was concentrated. To the concentrate were added brine and saturated aqueous sodium hydrogencarbonate solution, and the mixture was extracted with ethyl acetate. The extract was dried, and the solvent was evaporated under reduced pressure to give the title compound (56.3 g) as a colorless transparent oil.

CA 02418656 2003-02-06 ¹H-NMR(CDC1₃)01.41-1.45(9H,m), 1.95-2.34(2H,m)2.62-3.25(2H,m),

3.40-3.98(4H,m),4.40-4.90(4H,m).

Reference Example 10

Synthesis of 3-((2S,4S)-4-amino-l-tert-butoxycarbonyl-2pyrrolidinylcarbonyl)-1,3-thiazolidine (1) 3-((2S,4R)-l-tert-Butoxycarbonyl-4-hydroxy-2pyrrolidinylcarbonyl)-1,3-thiazolidine (title compound of Reference Example 9, 56.3 g) and triethylamine (28.5 mL) were dissolved in dichloromethane (1.0 1), and methanesulfonyl chloride (15.1 mL) was added thereto under ice-cooling. After stirring under ice-cooling for 1 hr, the reaction mixture was washed with water and dried. The solvent was evaporated under reduced pressure to give 3-((2S,4R)-l-tert-butoxycarbonyl-4methanesulfonyloxy-2-pyrrolidinylcarbonyl)-1,3-thiazolidine (70.5 g) as an oil.

(2) The above-mentioned compound (70.5 g) and sodium azide (13.3 g) were dissolved in DMF (500 mL), and the mixture was stirred at 80°C for 5 hr. The reaction mixture was concentrated under reduced pressure and water was added thereto. The mixture was extracted with ethyl acetate, and the organic layer was dried and concentrated. The residue was purified by silica gel chromatography to give 3-((2S,4S)-4-azido-l-tertbutoxycarbonyl-2-pyrrolidinylcarbonyl)-1,3-thiazolidine (39.5

g) as a white solid.

(3) The above-mentioned compound (26.7 g) was dissolved in ethanol (270 mL) and the mixture was stirred under a hydrogen atomosphere (1 atm) for 18 hr in the presence of 10% palladium carbon catalyst (13.4 g). The reaction mixture was filtrated and the filtrate was concentrated under reduced pressure to give the title compound (24.5 g) as a black solid.

¹H-NMR(CDC1₃)61.40-1.45(9H,m), 1.70-1.83(lH,m), 2.07(2H,brs), 2.32-2.56(lH,m), 2.90-3.19(2H,m), 3.25-3.58(2H,m), 3.604.14(3H,m), 4.31-4.80(3H,m).

CA 02418656 2003-02-06

Reference Example 11

Synthesis of 3-((2S,4S)-l-tert-butoxycarbonyl-4-hydroxy-2pyrrolidinylcarbonyl)-1,3-thiazolidine (1) N-tert-Butoxycarbonyl-L-cis-4-tert5 butyldimethylsilyloxyproline [product of Reference Example 1 (1) , 5.55 g] and thiazolidine (1.4 mL) were dissolved in DMF (55 mL), and triethylamine (2.24 mL), HOBT (2.96 g) and EDC hydrochloride (3.70 g) were successively added thereto. The mixture was stirred at room temperature for 13 hr. The io reaction mixture was added to saturated aqueous sodium hydrogencarbonate solution, and extracted with ethyl acetate. The extract was washed with water and brine and dried. The solvent was evaporated under reduced pressure and the residue was purified by silica gel chromatography to give 3-[(2S,4S)-115 tert-butoxycarbonyl-4-tert-butyldimethylsilyloxy-2pyrrolidinylcarbonyl]-l,3-thiazolidine (3.41 g) as a white solid.

(2) The above-mentioned compound (3.36 g) was dissolved in tetrahydrofuran (50 mL), and a 1.0 mol/L solution (9 mL) tetrabutylammonium fluoride in tetrahydrofuran was added dropwise under ice-cooling. The mixture was stirred at room temperature for 18 hr, and the solvent was evaporated under reduced pressure. The residue was added to brine and extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give the title compound (2.44 g) as a white solid.

¹ H-NMR(DMSO-d«)01.25-1.45(9H,m), 1.52-1.70(lH,m), 2.352.50(lH,m), 2.95-3.20(3H,m), 3.50-3.80(3H,m), 4.10-4.25(lH,m), 30 4.37-4.78(3H,m), 5.18(lH,brs).

Reference Example 12

Synthesis of 3-((S)-l-tert-butoxycarbonyl-4-oxo-2pyrrolidinylcarbonyl)-1,3-thiazolidine

CA 02418656 2003-02-06

The title compound (55.4 g) of Reference Example 9 and triethylamine (46 mL) were dissolved in dichloromethane (350 mL). A solution of sulfur trioxide pyridine complex (52.4 g) in dimethyl sulfoxide (150 mL) was added thereto under icecooling, and the mixture was stirred for 2 hr. Saturated aqueous sodium hydrogencarbonate solution was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The extract was washed with brine and dried. The solvent was evaporated under reduced pressure and the residue was purified by silica gel chromatography to give the title compound (30.3 g) as a white solid.

¹ H-NMR(CDC1₃)61.47(9H,S), 2.45-2.57( lH,m), 2.70-2.93( lH,m) , 2.97-3.22(2H,m), 3.66-3.78(0.6H,m), 3.80-4.10(3H,m), 4.284.38(0.4H,m), 4.45-5.08(3H,m).

Reference Example 13

Synthesis of (2S,4S)-1-tert-butoxycarbony1-4-(1indolyl)pyrrolidine-2-carboxylic acid (1) N-tert-Butoxycarbonyl-L-trans-4-hydroxyproline benzyl ester (20.3 g) and triethylamine (17.6 mL) were dissolved in dichloromethane (120 mL), and a solution of sulfur trioxide pyridine complex (25.1 g) in dimethyl sulfoxide (60 mL) was added thereto under ice-cooling, and the mixture was stirred for 5 hr. Saturated aqueous sodium hydrogencarbonate solution was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The extract was washed with brine and dried. The solvent was evaporated under reduced pressure and the residue was purified by silica gel chromatography to give benzyl (S)-1-tert-butoxycarbony1-4oxopyrrolidine-2-carboxylate (9.76 g) as an oil.

(2) The above-mentioned compound (2.50 g), indoline (1.05 mL) and acetic acid (0.45 mL) were dissolved in 1,2-dichloroethane (40 mL), and sodium triacetoxyborohydride (3.32 g) was added thereto. The mixture was stirred at room temperature for 6 hr

CA 02418656 2003-02-06 and saturated aqueous sodium hydrogencarbonate solution was added to the reaction mixture. The mixture was extracted with ethyl acetate, and the extract was washed with brine and dried. The solvent was evaporated under reduced pressure and the residue was purified by silica gel chromatography to give benzyl (2S,4S)-1-tert-butoxycarbony1-4-(1indolinyl)pyrrolidine-2-carboxylate (2.59 g) as an oil.

(3) The above-mentioned compound (2.53 g) was dissolved in acetone (50 mL) and manganese dioxide (7.51 g) was added thereto. The mixture was stirred at room temperature for 12 hr and the reaction mixture was filtrated. The filtrate was concentrated under reduced pressure and the residue was purified by silica gel chromatography to give benzyl (2S,4S)-1tert-butoxycarbonyl-4-(1-indolyl)pyrrolidine-2-carboxylate (2.43 g) as an oil.

(4) The above-mentioned compound (2.42 g) was dissolved in methanol (50 mL) and the mixture was stirred under a hydrogen atomosphere (1 atm) for 20 hr in the presence of 10% palladium/carbon (0.25 g). The reaction mixture was filtrated and the filtrate was concentrated under reduced pressure to give the title compound (1.91 g) as a pale-green solid. ^XH-NMR(CDC1₃)61.49(9H,S), 2.37-3.00(2H,m), 3.60-3.85(lH,m), 4.05-4.25(lH,m), 4.40-4.60(lH,m), 4.95-5.10(lH,m), 6.54(lH,s), 7.13(lH,t,J=7.7Hz), 7.18-7.28(2H,m), 7.38(lH,d,J=8.2Hz),

7.62(lH,d,J=7.7Hz).

Reference Example 14

Synthesis of 1-((S)-l-tert-butoxycarbonyl-4-oxo-2pyrrolidinylcarbonyl)pyrrolidine (1) N-tert-Butoxycarbonyl-L-trans-4-hydroxyproline (46.25 g) and pyrrolidine (18 mL) were dissolved in DMF (200 mL), and HOBT (45.5 g) and EDC hydrochloride (46.0 g) were successively added thereto. The mixture was stirred at room temperature for 13 hr, and the solvent was evaporated under reduced pressure.

CA 02418656 2003-02-06

Saturated aqueous sodium hydrogencarbonate solution was added to the residue, and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure to give 1-((2S,4R)-l-tert5 butoxycarbonyl-4-hydroxy-2-pyrrolidinylcarbonyl)pyrrolidine (59.3 g) as an oil.

(2) The above-mentioned compound (59.3 g) and triethylamine (41 mL) were dissolved in dichloromethane (350 mL), and a solution of sulfur trioxide pyridine complex (46.5 g) in dimethyl io sulfoxide (100 mL) was added thereto under ice-cooling. The mixture was stirred for 2 hr and saturated aqueous sodium hydrogencarbonate solution was added to the reaction mixture. The mixture was extracted with ethyl acetate, and the extract was washed with brine and dried. The solvent was evaporated is under reduced pressure. The residue was purified by silica gel chromatography to give the title compound (11.9 g) as a white solid.

¹H-NMR(CDC1₃)01.47(9H,s), 1.80-2.08(4H,m), 2.42-2.53(lH,m),

2.68-2.88(lH,m), 3.35-3.58(3H,m), 3.62-4.13(3H,m),

4.85(0.4H,d,J=9.0Hz), 4.99(0.6H,d,J=8.7Hz).

Reference Example 15

Synthesis of 3-((2S,4R)-4-amino-l-tert-butoxycarbonyl-2pyrrolidinylcarbonyl)-1,3-thiazolidine (1) The title compound (13.6 g) of Reference Example 11 was dissolved in dichloromethane (250 mL), and triethylamine (7 mL) was added thereto. Methanesulfonyl chloride (3.64 mL) was added to the reaction mixture under ice-cooling, and the mixture was stirred for 1 hr. Water was added to the reaction mixture and the organic layer was separated. The solvent was evaporated under reduced pressure and the residue was dissolved in DMF (200 mL). Sodium azide (3.25 g) was added thereto and the mixture was stirred at 85°C for 3 hr with heating. The reaction mixture was concentrated under reduced pressure and

CA 02418656 2003-02-06 water was added to the residue. The mixture was extracted with ethyl acetate and the extract was concentrated under reduced pressure. The residue was purified by silica gel chromatography to give 3-((2S,4R)-4-azido-l-tertbutoxycarbonyl-2-pyrrolidinylcarbonyl)-1,3-thiazolidine (9.9 g). (2) The above-mentioned compound (9.9 g) was dissolved in ethanol (150 mL) and the mixture was stirred at room temperature for 18 hr under a hydrogen atomosphere (1 atm) in the presence of 10% palladium/carbon (10 g). The reaction mixture was filtrated and the filtrate was concentrated under reduced pressure to give the title compound (10.1 g) as a black solid.

¹ H-NMR(500MHz,DMSO-de)01.30(4.5H,S), 1.38(4.5H,S), 1.421.52(lH,m), 2.05(2H,brs), 2.33-2.45(lH,m), 2.92-3.88(7H,m),

4.37-4.72(3H,m).

Example 1

Synthesis of (S)-2-cyano-l-((2S,4S)-4-hydroxy-2pyrrolidinylcarbony1)pyrrolidine trifluoroacetate (S)-1-((2S,4S)-l-tert-Butoxycarbonyl-4-hydroxy-2pyrrolidinylcarbonyl)-2-cyanopyrrolidine (title compound of Reference Example 1, 544 mg) was dissolved in dichloromethane (18 mL), and trifluoroacetic acid (1.8 mL) was added thereto. The mixture was stirred at room temperature for 18 hr, and the solvent was evaporated under reduced pressure. Ethanol and diethyl ether were added to the residue and the precipitated powder was collected by filtration to give the title compound (350 mg) as powdery white crystals.

¹H-NMR(DMSO-de)01.68-2.85(6H,m), 3.10-3.30(2H,m), 3.443.70(2H,m), 4.30-4.60(2H,m), 4.78-5.09(lH,m), 5.35-5.50(lH,m), 9.18(2H,brs).

Example 2

Synthesis of (S)-2-cyano-l-((2S,4R)-4-hydroxy-2pyrrolidinylcarbony1)pyrrolidine hydrochloride

CA 02418656 2003-02-06

The title compound (309 mg) of Reference Example 2 was dissolved in 4 mol/L hydrochloric acid-1,4-dioxane (2 mL), and the mixture was stirred at room temperature for 18 hr. The solvent was evaporated under reduced pressure and the residue was purified by HPLC to give the title compound (36 mg) as powdery white crystals.

¹ H-NMR(DMSO-d₆ )01.85-2.44(6H,m), 3.05-3.72(4H,m), 4.414.60(2H,m), 4.78-5.16(lH,m), 5.55-5.70(lH,m).

Example 3

Synthesis of (S)-1-((2S,4S)-4-amino-2-pyrrolidinylcarbonyl)-2cyanopyrrolidine dihydrochloride (S)-1-((2S,4S)-4-Amino-l-tert-butoxycarbonyl-2pyrrolidinylcarbonyl)-2-cyanopyrrolidine (title compound of Reference Example 3, 308 mg) was dissolved in 4 mol/L hydrochloric acid-1,4-dioxane (1.25 mL), and the mixture was stirred at room temperature for 27 hr. The solvent was evaporated under reduced pressure and tetrahydrofuran was added thereto. The precipitated solid was collected by filtration to give the title compound (214 mg).

¹ H-NMR(DMSO-d6)01.85-2.35(5H,m), 2.80-2.93(lH,m), 3.273.68(4H,m), 3.83-3.98(lH,m), 4.52-4.64(lH,m), 4.84(lH,d,J=4.5,7.8Hz), 8.81(3H,brs).

Example 4

Synthesis of (S)-l-((2S,4S)-4-anilino-2-pyrrolidinylcarbonyl)2-cyanopyrrolidine trifluoroacetate (1) The title compound (6.18 g) of Reference Example 2 and triethylamine (8.4 mL) were dissolved in dimethyl sulfoxide (15 mL). Sulfur trioxide pyridine complex (9.54 g) was added thereto. After stirring at room temperature for 1 hr, water was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The extract was washed with water and dried. The solvent was evaporated under reduced pressure and the residue was purified by silica gel

CA 02418656 2003-02-06 chromatography to give (S)-l-((2S)-l-tert-butoxycarbonyl-4-oxo2-pyr^rolidinyl^carb^onyi)-2-cyan^opyrrolidine (6.0 g) as an oil.

(2) The above-mentioned compound (1.5 g) and aniline (0.43 mL) were dissolved in methanol (25 mL), and the mixture was stirred at room temperature for 6 hr in the presence of molecular sieve 3A (1.5 g). Sodium cyanoborohydride (0.315 g) and acetic acid (0.4 mL) were added to the reaction mixture, and the mixture was stirred for 1 hr. The reaction mixture was filtrated and the filtrate was evaporated under reduced pressure. Saturated aqueous sodium hydrogencarbonate solution was added to the residue, and the mixture was extracted with ethyl acetate. The extract was dried, and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-1-((2S,4S)-4-anilino-1-tert- butoxycarbony1-2-pyrrolidinylcarbonyl)-2-cyanopyrrolidine (0.7 4 g) as amorphous.

(3) The above-mentioned compound (0.82 g) was dissolved in dichloromethane (21 mL) and trifluoroacetic acid (2.1 mL) was added thereto. The mixture was stood at room temperature for 15 hr, and the solvent was evaporated under reduced pressure. The residue was purified by HPLC and freeze-dried to give the title compound (0.246 g) as amorphous.

¹ H-NMR(DMSO-dg)Ô1.52—2.33(5H,m), 2.87-3.22(2H,m), 3.755.13(8H,m), 6.50-6.67(3H,m), 7.03-7.20(2H,m), 8.91(lH,brs), 9.88(lH,brs).

Example 5

Synthesis of (S)-2-cyano-l-[(2S,4S)-4-(4-nitrophenyl)amino-2pyrrolidinylcarbonyl]pyrrolidine hydrochloride (1) The title compound (0.924 g) of Reference Example 3, diisopropylethylamine (1.7 mL) and 4-fluoronitrobenzene (0.53 mL) were dissolved in N-methyl-2-pyrrolidone (10 mL), and the mixture was stirred at 80°C for 15 hr. Water was added to the reaction mixture and the mixture was extracted with ethyl

CA 02418656 2003-02-06 acetate. The extract was dried, and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-1-[(2S,4S)-1-tert-butoxycarbony1-4(4-nitrophenyl)amino-2-pyrrolidinylcarbonyl]-2-cyanopyrrolidine (1.14 g) as amorphous.

(2) The above-mentioned compound (1.13 g) was dissolved in ethyl acetate (3.5 mL), and 4 mol/L hydrochloric acid-ethyl acetate (3.4 mL) was added thereto. The mixture was stood at room temperature for 15 hr. The precipitated solid was collected by filtration to give the title compound (0.67 g).

¹ H-NMR(DMSO-de)01.74-2.37(6H,m), 2.90-3.10(lH,m), 3.273.97(3H,m), 4.37-4.70(2H,m), 4.80-5.20(lH,m), 6.90-7.34(2H,m),

7.88-8.03(2H,m), 9.09(lH,brs), 10.98(1H,brs).

Example 6

Synthesis of (S)-2-cyano-l-[(2S,4S)-4-(4-cyanophenyl)amino-2pyrrolidinylcarbonyl]pyrrolidine hydrochloride (1) The title compound (924 mg) of Reference Example 3, diisopropylethylamine (1.7 mL) and 4-fluorobenzonitrile (606 mg) were dissolved in N-methyl-2-pyrrolidone (10 mL), and the mixture was stirred at 100°C for 15 hr. Water was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-2-cyano-l-[(2S,4S)-l-tertbutoxycarbonyl-4-(4-cyanophenyl)amino-2pyrrolidinylcarbonyl]pyrrolidine (340 mg) as amorphous.

(2) The above-mentioned compound (420 mg) was dissolved in ethyl acetate (1.2 mL), and 4 mol/L hydrochloric acid-ethyl acetate (1.2 mL) was added thereto. The mixture was stood at room temperature for 15 hr and the precipitated solid was collected by filtration to give the title compound (289 mg).

¹ H-NMR(DMSO-de)61.62-1.80(lH,m), 1.97-2.33(4H,m), 2.903.24(2H,m), 3.64-3.96(3H,m), 4.20-4.63(2H,m), 4.80-5.13(lH,m),

CA 02418656 2003-02-06

6.70(2H,d,J=8.7Hz), 7.53(2H,d,J=8.7Hz), 9.00(lH,brs),

10.50(1H,brs).

Example 7

Synthesis of (S)-2-cyano-l-[(2S,4S)-4-(2-cyanophenyl)amino-2pyrrolidinylcarbonyl]pyrrolidine hydrochloride (1) The title compound (924 mg) of Reference Example 3, diisopropylethylamine (1.7 mL) and 2-fluorobenzonitrile (0.54 mL) were dissolved in N-methyl-2-pyrrolidone (10 mL), and the mixture was stirred at 100°C for 15 hr. Water was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-l-[(2S,4S)-l-tert-butoxycarbonyl-4(2-cyanophenyl)amino-2-pyrrolidinylcarbonyl]-2-cyanopyrrolidine (177 mg) as amorphous.

(2) The above-mentioned compound (115 mg) was dissolved in ethyl acetate (0.5 mL), and 4 mol/L hydrochloric acid-ethyl acetate (0.35 mL) was added thereto. The mixture was stood at room temperature for 15 hr. The precipitated solid was collected by filtration to give the title compound (46 mg).

¹H-NMR(DMSO-d₆ )01.70-2.36(6H,m), 2.93-3.74(3H,m), 4.284.66(2H,m), 4.81-5.15(lH,m), 6.33(lH,d,J=8.1Hz),

6.77(lH,dd,J=8.4,8.1Hz), 6.90(lH,d,J=8.4Hz), 7.39-7.60(2H,m). Example 8

Synthesis of (S)-2-cyano-1-((2S,4S)-4-(2-fluoro-4nitrophenyl)amino-2-pyrrolidinylcarbonyl]pyrrolidine hydrochloride (1) The title compound (0.924 g) of Reference Example 3, diisopropylethylamine (1.7 mL) and 3,4-difluoronitrobenzene (0.55 mL) were dissolved in N-methy1-2-pyrrolidone (10 mL), and the mixture was stirred at 80°C for 15 hr. Water was added to the reaction mixture and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated

CA 02418656 2003-02-06 under reduced pressure. The residue was purified by silica gel chromatography to give (S)-l-[(2S,4S)-l-tert-butoxycarbonyl-4(2-fluoro-4-nitrophenyl)amino-2-pyrrolidinylcarbonyl]-2cyanopyrrolidine (0.95 g) as amorphous.

(2) The above-mentioned compound (0.95 g) was dissolved in ethyl acetate (3 mL), and 4 mol/L hydrochloric acid-ethyl acetate (2.65 mL) was added thereto. The mixture was stood at room temperature for 15 hr and the precipitated solid was collected by filtration to give the title compound (0.63 g).

¹ H-NMR(DMSO-d₆ )01.74-2.37(6H,m), 2.90-3.10(lH,m), 3.273.97(3H,m), 4.37-4.70(2H,m), 4.80-5.20(lH,m), 6.90-7.34(2H,m),

7.88-8.03(2H,m), 9.09(lH,brs), 10.98(lH,brs).

Example 9

Synthesis of (S)-2-cyano-1-((2S,4S)-4-(4-cyano-2fluorophenyl)amino-2-pyrrolidinylcarbonyl]pyrrolidine hy droc hlor ide (1) The title compound (924 mg) of Reference Example 3, diisopropylethylamine (1.7 mL) and 3,4-difluorobenzonitrile (700 mg) were dissolved in N-methy1-2-pyrrolidone (10 mL), and the mixture was stirred at 80°C for 15 hr. Water was added to the reaction mixture and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)—1—[(2S,4S)-l-tert-butoxycarbonyl-4(4-cyano-2-fluorophenyl)amino-2-pyrrolidinylcarbonyl]-2cyanopyrrolidine (920 mg) as amorphous.

(2) The above-mentioned compound (920 mg) was dissolved in ethyl acetate (2.5 mL), and 4 mol/L hydrochloric acid-ethyl acetate (2.2 mL) was added thereto. The mixture was stood at room temperature for 15 hr and the precipitated solid was collected by filtration to give the title compound (756 mg).

¹ H-NMR(DMSO-de)01.67-2.35(6H,m), 2.88-3.06(lH,m), 3.223.73(3H,m), 4.27-4.64(2H,m), 4.86-5.13(lH,m),

CA 02418656 2003-02-06

6.73(lH,d,J=6.3Hz), 7.82-7.92(lH,m), 7.51(1H,dd,J=8.4,1.5Hz),

7.61(lH,dd,J=12.0,1.5Hz), 8.98(lH,brs), 10.75(lH,brs).

Example 10

Synthesis of (S)-1-[(2S,4S)-4-(4-bromo-2-cyanophenyl)amino-2pyrrolidinylcarbonyl]-2-cyanopyrrolidine hydrochloride (1) The title compound (924 mg) of Reference Example 3, diisopropylethylamine (1.7 mL) and 3-bromo-6-fluorobenzonitrile (1000 mg) were dissolved in N-methyl-2-pyrrolidone (10 mL) and the mixture was stirred at 80°C for 5 hr. Water was added to the reaction mixture and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-l-[(2S,4S)-l-tert-butoxycarbonyl-4(4-bromo-2-cyanophenyl)amino-2-pyrrolidinylcarbonyl]-2- cyanopyrrolidine (904 mg) as amorphous.

(2) The above-mentioned compound (900 mg) was dissolved in ethyl acetate (2 mL) and 4 mol/L hydrochloric acid-ethyl acetate (1.8 mL) was added thereto. The mixture was stood at room temperature for 15 hr and the precipitated solid was collected by filtration to give the title compound (501 mg).

¹ H-NMR(DMSO-de)01.70-2.34(4H,m), 2.93-3.07(lH,m), 3.423.65(3H,m), 4.33-4.61(2H,m), 4.82-5.10(lH,m),

6.56(lH,d,J=8.7Hz), 6.94(lH,dd,J=8.1,1.8Hz), 7.12(lH,d,J=1.8Hz), 7.49(lH,d,J=8.1Hz), 8.96(lH,brs), 10.02(lH,brs).

Example 11

Synthesis of (S)-2-cyano-l-[(2S,4S)-4-(3,4-dicyanophenyl)amino2-pyrrolidinylcarbonyl]pyrrolidine hydrochloride (1) The title compound (0.924 g) of Reference Example 3, diisopropylethylamine (1.7 mL) and 3-fluorophthalonitrile (0.73 g) were dissolved in N-methyl-2-pyrrolidone (10 mL), and the mixture was stirred at 80°c for 4 hr. Water was added to the reaction mixture and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated

CA 02418656 2003-02-06 under reduced pressure. The residue was purified by silica gel chromatography to give (S)-l-[(2S,4S)-l-tert-butoxycarbonyl-4(3,4-dicyanophenyl)amino-2-pyrrolidinylcarbonyl] -2cyanopyrrolidine (1.05 g) as amorphous.

(2) The above-mentioned compound (1.04 g) was dissolved in ethyl acetate (5 mL) and 4 mol/L hydrochloric acid-ethyl acetate (3.0 mL) was added thereto. The mixture was stood at room temperature for 15 hr and the precipitated solid was collected by filtration to give the title compound (0.794 g).

io ¹ H-NMR(DMSO-ds)ôl.62-1.79(lH,m), 1.95-2.35(3H,m), 2.903.28(2H,m), 3.53-3.71(2H,m), 4.23-4.64(2H,m), 4.80-5.13(lH,m), 7.01(lH,dd,J=9.0,2.4Hz), 7.24(lH,d,J=2.4Hz), 7.65(lH,d,J=6.3Hz), 7.78(lH,d,J=9.0Hz), 9.06(lH,brs), 10.62(1H,brs).

Example 12 is Synthesis of (S)-1-((2S,4S)-4-(3-chloro-4-cyanophenyl)amino-2pyrrolidinylcarbonyl]-2-cyanopyrrolidine hydrochloride (1) The title compound (0.924 g) of Reference Example 3, diisopropylethylamine (1.7 mL) and 2-chloro-4fluorobenzonitrile (0.78 g) were dissolved in N-methyl-2- pyrrolidone (10 mL), and the mixture was stirred at 80°C for 4 hr. Water was added to the reaction mixture and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)—1— [(2S,4S)-l-tert-butoxycarbonyl-4-(3-chloro-4-cyanophenyl)amino2-pyrrolidinylcarbonyl]-2-cyanopyrrolidine (0.94 g) as amorphous.

(2) The above-mentioned compound (0.93 g) was dissolved in ethyl acetate (3 mL) and 4 mol/L hydrochloric acid-ethyl acetate (2.6 mL) was added thereto. The mixture was stood at room temperature for 15 hr and the precipitated solid was collected by filtration to give the title compound (0.73 g).

¹ H-NMR(DMSO-d₆ )01.58-1.80(lH,m), 1.96-2.37(3H,m), 2.9250

CA 02418656 2003-02-06

3.22(2H,m), 3.53-3.67(2H,m), 4.22-4.60(2H,m), 4.80-5.10(lH,m),

6.67(lH,dd,J=8.7,2.4Hz), 6.84(lH,d,J=2.4Hz), 7.24(lH,d,J=6.9Hz),

7.61(lH,d,J=8.7Hz), 9.02(lH,brs), 9.97(lH,brs).

Example 13

Synthesis of (S)-2-cyano-l-[(2S,4S)-4-(4-cyano-2,6difluoropheny1)amino-2-pyrrolidinylcarbony1]pyrrolidine hydrochloride (1) The title compound (0.924 g) of Reference Example 3, diisopropylethylamine (1.7 mL) and 3,4,5-trifluorobenzonitrile (0.79 g) were dissolved in N-methy1-2-pyrrolidone (10 mL), and the mixture was stirred at 80°C for 3 hr. Water was added to the reaction mixture and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)—1—[(2S,4S)-l-tert-butoxycarbonyl-4- (4-cyano-2,6-difluoropheny1)amino-2-pyrrolidinylcarbonyl]-2cyanopyrrolidine (1.08 g) as amorphous.

(2) The above-mentioned compound (1.08 g) was dissolved in ethyl acetate (3 mL) and 4 mol/L hydrochloric acid-ethyl acetate (2.4 mL) was added thereto. The mixture was stood at room temperature for 15 hr and the precipitated solid was collected by filtration to give the title compound (0.706 g). ¹H-NMR(DMSO-d₆)01.72-2.33(6H,m), 2.77-2.92(lH,m), 3.203.70(3H,m), 4.42-5.09(3H,m), 6.38(lH,d,J=8.4Hz), 7.517.70(2H,m), 8.97(lH,brs), 10.73(lH,brs).

Example 14

Synthesis of (S)-2-cyano-l-[(2S,4S)-4-(3-trifluoromethyl-2pyridyl)amino-2-pyrrolidinylcarbonyl]pyrrolidine trifluoroacetate (1) The title compound (1.86 g) of Reference Example 3, diisopropylethylamine (3.14 mL) and 2-chloro-3trifluoromethylpyridine (1.09 g) were dissolved in N-methy1-2pyrrolidone (36 mL), and the mixture was stirred at 120°C for 8

CA 02418656 2003-02-06 hr. The reaction mixture was added to saturated aqueous sodium hydrogencarbonate solution, and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)—1—[(2S,4S)-l-tertbutoxycarbonyl-4-(3-trifluoromethy1-2-pyridyl)amino-2pyrrolidinylcarbonyl]-2-cyanopyrrolidine (0.099 g).

(2) The above-mentioned compound (99 mg) was dissolved in dichloromethane (2.2 mL) and trifluoroacetic acid (0.2 mL) was added thereto. The mixture was stood at room temperature for 18 hr. The reaction mixture was concentrated under reduced pressure and purified by HPLC to give the title compound (3.2 mg) as amorphous.

¹ H-NMR(DMSO-d₆ )01.80-2.40(5H,m), 2.80-2.98(lH,m), 3.303.65(4H,m), 4.40-4.78(lH,m), 4.80-4.89(lH,m), 4.90-5.10(lH,m), 6.58(lH,d,J=7.8Hz), 6.73-6.89(lH,m), 7.86(lH,d,J=6.6Hz), 8.33(lH,d,J=3.9Hz), 8.84(lH,brs), 9.56(lH,brs).

Example 15

Synthesis of (S)-2-cyano-l-[(2S,4S)-4-(3-nitro-2-pyridyl)amino2-pyrrolidinylcarbonyl]pyrrolidine trifluoroacetate (1) The title compound (928 mg) of Reference Example 3, diisopropylethylamine (1.57 mL) and 2-chloro-3-nitropyridine (476 mg) were dissolved in N-methy1-2-pyrrolidone (18 mL), and the mixture was stirred at 80°C for 3 hr. The reaction mixture was added to saturated aqueous sodium hydrogencarbonate solution, and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-1-((2S,4S)-l-tert-butoxycarbonyl-4(3-nitro-2-pyridyl) amino-2-pyrrolidinylcarbonyl]-2cyanopyrrolidine (851 mg) as a yellow oil.

(2) The above-mentioned compound (851 mg) was dissolved in dichloromethane (20 mL) and trifluoroacetic acid (2.0 mL) was

CA 02418656 2003-02-06 added thereto. The mixture was stood at room temperature for hr. The reaction mixture was concentrated under reduced pressure and diethyl ether was added to the residue. The precipitated product was collected by filtration to give a yellow powder (677 mg). This powder (257 mg) was purified by HPLC to give the title compound (152 mg) as a yellow powder.

¹ H-NMR(DMSO-cU )01.89-2.37(5H,m), 2.86-3.04(lH,m), 3.383.65(4H,m), 4.49-4.70(lH,m), 4.79-4.90(lH,m), 5.00-5.22(lH,m), 6.88(lH,dd,J=8.4,4.8Hz), 8.38-8.60(3H,m), 9.03(1H,brs), 9.73(lH,brs).

Example 16

Synthesis of (S)-2-cyano-l-[(2S,4S)-4-(3-cyano-2-pyridyl)amino2-pyrrolidinylcarbonyl]pyrrolidine hydrochloride (1) The title compound (1.86 g) of Reference Example 3, diisopropylethylamine (3.14 mL) and 2-chloro-3-cyanopyridine (0.831 g) were dissolved in N-methyl-2-pyrrolidone (36 mL), and the mixture was stirred at 100°C for 9 hr. The reaction mixture was added to saturated aqueous sodium hydrogencarbonate solution, and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-l-[(2S,4S)-l-tert-butoxycarbonyl-4- (3-cyano-2-pyridyl)amino-2-pyrrolidinylcarbonyl]-2cyanopyrrolidine (0.487 g).

(2) The above-mentioned compound (0.464 g) was dissolved in ethyl acetate (1.13 mL) and 4 mol/L hydrochloric acid-ethyl acetate (1.41 mL) was added thereto. The mixture was stood at room temperature for 3 hr. The precipitated solid was collected by filtration to give the title compound (0.370 g) as a pale-brown powder.

¹ H-NMR(DMSO-d6)01.72-2.31(5H,m), 2.81-2.98(lH,m), 3.203.36(lH,m), 3.40-3.69(3H,m), 4.45-4.67(lH,m), 4.75-5.11(lH,m), 6.78(lH,dd,J=7.5,4.8Hz), 7.35(lH,d,J=7.5Hz),

CA 02418656 2003-02-06

7.99(lH,dd,J=7.5,1.8Hz), 8.33(lH,dd,J=5.1,1.8Hz), 8.88(lH,brs),

10.50(lH,brs).

Example 17

Synthesis of (S)-2-cyano-l-[(2S,4S)-4-(4-cyano-2-pyridyl)amino2-pyrrolidinylcarbonyl]pyrrolidine trifluoroacetate (1) The title compound (928 mg) of Reference Example 3, diisopropylethylamine (1.57 mL) and 2-chloro-4-cyanopyridine (416 mg) were dissolved in N-methyl-2-pyrrolidone (18 mL), and the mixture was stirred at 120°C for 18 hr. The reaction mixture was added to saturated aqueous sodium hydrogencarbonate solution, and extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-1-[(2S,4S)-1-tert-butoxycarbonyl-4-(4-cyano-2pyridyl)amino-2-pyrrolidinylcarbonyl]-2-cyanopyrrolidine (134 mg).

(2) The above-mentioned compound (134 mg) was dissolved in dichloromethane (3.3 mL) and trifluoroacetic acid (0.3 mL) was added thereto. The mixture was stirred at room temperature for 18 hr. The reaction mixture was concentrated under reduced pressure and diethyl ether was added to the residue. The precipitated product was collected by filtration to give a pale-brown powder (64 mg). This powder (64 mg) was purified by HPLC to give the title compound (8 mg) as a white solid.

¹ H-NMR(DMSO-ds)01.64-1.86(lH,m), 1.86-2.37(4H,m), 2.803.00(lH,m), 3.06-3.28(lH,m), 3.42-3.69(3H,m), 4.42-4.70(2H,m), 4.75-5.10(lH,m), 6.89(lH,s), 6.93(lH,d,J=5.2Hz), 7.44(lH,d,J=6.2Hz), 8.23(lH,d,J=4.7Hz), 8.96(lH,brs), 9.81(lH,brs).

Example 18

Synthesis of (S)-2-cyano-l-[(2S,4S)-4-(5-cyano-2-pyridyl)amino2-pyrrolidinylcarbonyl]pyrrolidine dihydrochloride (1) The title compound (462 mg) of Reference Example 3,

CA 02418656 2003-02-06 triethylamine (0.42 mL) and 2-chloro-5-cyanopyridine (210 mg) were dissolved in DMF (10 mL), and the mixture was stirred at 90°C for 24 hr. Water was added to the reaction mixture and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-1-[(2S,4S)-l-tert-butoxycarbonyl-4-(5-cyano-2pyridyl)amino-2-pyrrolidinylcarbonyl]-2-cyanopyrrolidine (310 mg) as amorphous.

(2) The above-mentioned compound (310 mg) was dissolved in tetrahydrofuran (3 mL) and 4 mol/L hydrochloric acid-ethyl acetate (2 mL) was added thereto. The mixture was stood at room temperature for 15 hr and the solvent was evaporated under reduced pressure. The residue was purified by HPLC and freezedried to give the title compound (150 mg) as amorphous.

¹ H-NMR(DMSO-de)Ô1.77-2.33(2.81-3.23(2H,m), 4.00-5.15(6H,m), 6.61(lH,d,J=8.7Hz), 7.77(lH,d,J=8.7Hz), 7.91(lH,d,J=6.0Hz), 8.46(lH,s), 8.98(lH,brs), 9.91(lH,brs).

Example 19

Synthesis of (S)-l-[(2S,4R)-4-(5-cyano-2-pyridyl)amino-2pyrrolidinylcarbonyl]-2-cyanopyrrolidine dihydrochloride (1) (2S,4R)-l-tert-Butoxycarbonyl-4-(5-cyano-2pyridyl)aminopyrrolidine-2-carboxylic acid (title compound of Reference Example 4, 0.73 g) and (S)-2-cyanopyrrolidine hydrochloride (0.29 g) were dissolved in DMF (10 mL), and triethylamine (0.62 mL), HOBT (0.34 g) and EDC hydrochloride (0.42 g) were successively added thereto. The mixture was stirred at room temperature for 15 hr. Water was added to the reaction mixture and the mixture was extracted with ethyl acetate. The extract was dried and evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-1-((2S,4S)-1-tert-butoxycarbony1-4(5-cyano-2-pyridyl)amino-2-pyrrolidinylcarbonyl]-255

CA 02418656 2003-02-06 cyanopyrrolidine (0.58 g) as amorphous.

(2) The above-mentioned compound (0.57 g) was dissolved in 4 mol/L hydrochloric acid-1,4-dioxane (4 mL), and the mixture was stood at room temperature for 15 hr. The solvent was evaporated under reduced pressure, and the residue was purified by HPLC and freeze-dried to give the title compound (0.181 g) as amorphous.

¹H-NMR(DMSO-de)01.93-2.68(6H,m), 3.13-3.70(4H,m), 4.585.10(3H,m), 6.61(lH,d,J=8.7Hz), 7.79(lH,dd,J=8.7,1-8Hz),

7.98(lH,brs), 8.48(lH,d,J=1.8Hz), 8.87(lH,brs), 9.78(lH,brs). Example 20

Synthesis of (S)-2-cyano-l-[(2S,4S)-4-(5-trifluoromethyl-2pyridy1)amino-2-pyrrolidinylcarbonyl]pyrrolidine 2 trifluoroacetate is (1) The title compound (0.924 g) of Reference Example 3, diisopropylethylamine (1.7 mL) and 2-chloro-5trifluoromethylpyridine (0.54 g) were dissolved in N-methyl-2pyrrolidone (10 mL), and the mixture was stirred at 120°C for 8 hr. Water was added to the reaction mixture and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-l[(2S,4S)-l-tert-butoxycarbonyl-4-(5-trifluoromethyl-2pyridyl)amino-2-pyrrolidinylcarbonyl]-2-cyanopyrrolidine (0.40

g) as amorphous.

(2) The above-mentioned compound (0.40 g) was dissolved in dichloromethane (9 mL) and trifluoroacetic acid (0.9 mL) was added thereto. The mixture was stood at room temperature for 15 hr, and the solvent was evaporated under reduced pressure.

The residue was purified by HPLC and freeze-dried to give the title compound (0.163 g) as amorphous.

¹H-NMR(DMSO-d₆)01.68-2.32(5H,m), 2.85-3.26(2H,m), 4.385.12(8H,m), 6.65(lH,d,J=9.0Hz), 7.66(lH,d,J=6.3Hz),

CA 02418656 2003-02-06

7.72(lH,dd,J=9.0,2.4Hz), 8.35(lH,s), 8.96(lH,brs), 9.80(lH,brs).

Example 21

Synthesis of (S)-2-cyano-l-[(2S,45)-4-(5-nitro-2-pyridyl)amino2-pyrrolidinylcarbonyl]pyrrolidine dihydrochloride (1) The title compound (0.462 g) of Reference Example 3, triethylamine (0.42 mL) and 2-chloro-5-nitropyridine (0.24 g) were dissolved in DMF (6 mL), and the mixture was stirred at 100°C for 8 hr. Water was added to the reaction mixture and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-1-[(2S,4S)-l-tert-butoxycarbonyl-4-(5-nitro-2pyridy1)amino-2-pyrrolidinylcarbonyl]-2-cyanopyrrolidine (0.50 g) as amorphous.

(2) The above-mentioned compound (0.50 g) was dissolved in tetrahydrofuran (3 mL) and 4 mol/L hydrochloric acid-1,4dioxane (3 mL) was added thereto. The mixture was stood at room temperature for 15 hr and the precipitated solid was collected by filtration to give the title compound (0.445 g).

¹ H-NMR(DMSO-de)01.69-2.37(5H,m), 2.80-3.26(2H,m), 3.473.76(3H,m), 4.50-5.15(3H,m), 6.67(lH,d,J=9.3Hz), 8.18(lH,dd,J=9.3,3.0Hz), 8.58(lH,s), 8.90-9.12(2H,m), 10.65(lH,brs).

Example 22

Synthesis of (S)-2-cyano-l-[(2S,4S)-4-(3,5-dinitro-2pyridy1)amino-2-pyrrolidinylcarbonyl]pyrrolidine trifluoroacetate (1) The title compound (0.928 g) of Reference Example 3, diisopropylethylamine (1.57 mL) and 2-chloro-3,5dinitropyridine (0.611 g) were dissolved in N-methyl-2pyrrolidone (18 mL), and the mixture was stirred at room temperature for 2 hr. The reaction mixture was added to saturated aqueous sodium hydrogencarbonate solution, and the

CA 02418656 2003-02-06 mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-1-[(2S,4S)-l-tert-butoxycarbonyl-4-(3,5-dinitro-2pyridyl)amino-2-pyrrolidinylcarbony1]-2-cyanopyrrolidine (1.21 g) as a yellow oil.

(2) The above-mentioned compound (1.18 g) was dissolved in dichloromethane (25 mL) and trifluoroacetic acid (2.5 mL) was added thereto. The mixture was stirred at room temperature for 18 hr. The reaction mixture was concentrated under reduced pressure and diethyl ether was added to the residue. The precipitated yellow powder was collected by filtration to give the title compound (1.12 g).

¹ H-NMR(DMSO-d₆ )01.68-2.33{5H,m), 2.84-3.08(lH,m), 3.323.68(4H,m), 4.49-4.70(lH,m), 4.76-4.90(lH,m), 5.11-5.37(lH,m), 9.01(lH,s), 9.04(lH,brs), 9.25(lH,brs), 9.28(lH,s), 9.84(lH,brs).

Example 23

Synthesis of (S)-1-[(2S,4S)-4-(6-chloro-3-pyridazinyl)amino-2pyrrolidinylcarbonyl]-2-cyanopyrrolidine hydrochloride (1) The title compound (928 mg) of Reference Example 3, diisopropylethylamine (1.57 mL) and 2,6-dichloropyridazine (447 mg) were dissolved in N-methyl-2-pyrrolidone (18 mL), and the mixture was stirred at 120°C for 5 hr. The reaction mixture was added to saturated aqueous sodium hydrogencarbonate solution, and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-l-[(2S,4S)-l-tert-butoxycarbonyl-4- (6-chloro-3-pyridazinyl)amino-2-pyrrolidinylcarbonyl]-2cyanopyrrolidine (58 mg).

(2) The above-mentioned compound (57 mg) was dissolved in ethyl acetate (1.0 mL) and 4 mol/L hydrochloric acid-ethyl acetate

CA 02418656 2003-02-06 (0.2 mL) was added thereto. The mixture was stood at room temperature for 4 hr and the precipitated solid was collected by filtration to give the title compound (31 mg).

¹H-NMR(DMSO-d₆)011.69-2.35(5H,m), 2.88-3.01(lH,m), 3.093.29(lH,m), 3.50-3.70(3H,m), 4.50-4.72(2H,m), 4.76-5.15(lH,m), 7.00(lH,d,J=9.3Hz), 7.48(lH,d,J=9.3Hz), 7.71(lH,brs), 9.00(lH,brs), 10.31(lH,brs).

Example 24

Synthesis of (S)-2-cyano-l-[(2S,4S)-4-(2-pyrimidinyl)amino-2pyrrolidinylcarbonyl]pyrrolidine hydrochloride (1) The title compound (1.85 g) of Reference Example 3, diisopropylethylamine (3.14 mL) and 2-chloropyrimidine (0.687 g) were dissolved in N-methyl-2-pyrrolidone (30 mL), and the mixture was stirred at 100°C for 24 hr. The reaction mixture was added to saturated aqueous sodium hydrogencarbonate solution, and extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-1-[(2S,4S)-l-tert-butoxycarbonyl-4-(2-pyrimidinyl)amino-2pyrrolidinylcarbonyl]-2-cyanopyrrolidine (0.950 g) as a palebrown powder.

(2) The above-mentioned compound (0.833 g) was dissolved in ethyl acetate (2.16 mL) and 4 mol/L hydrochloric acid-ethyl acetate (2.69 mL) was added thereto. The mixture was stood at room temperature for 18 hr. The precipitated solid was collected by filtration to give the title compound (0.626 g).

¹H-NMR(DMSO-de)01.70-2.35(5H,m), 2.80-2.95(lH,m), 3.103.30(lH,m), 3.42-3.90(3H,m), 4.49-4.73(2H,m), 4.80-5.14(lH,m), 6.74(1H,t,J=5.1Hz), 7.67(lH,brs), 8.38(2H,d,J=5.1Hz), 8.89(lH,brs), 10.37(lH,brs).

Example 25

Synthesis of (S)-2-cyano-l-[(2S,4S)-4-(4-trifluoromethy1-2pyrimidiny1)amino-2-pyrrolidinylcarbonyl]pyrrolidine

CA 02418656 2003-02-06 hydrochloride (1) The title compound (1.85 g) of Reference Example 3, diisopropylethylamine (3.14 mL) and 2-chloro-4trifluoromethylpyrimidine (1.10 g) were dissolved in N-methyl2-pyrrolidone (30 mL), and the mixture was stirred at room temperature for 18 hr. The reaction mixture was added to saturated aqueous sodium hydrogencarbonate solution, and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-1-[(2S,4S)-l-tert-butoxycarbonyl-4-(4-trifluoromethyl-2pyrimidinyl)amino-2-pyrrolidinylcarbonyl]-2-cyanopyrrolidine (2.44 g).

(2) The above-mentioned compound (2.27 g) was dissolved in ethyl acetate (4.99 mL) and 4 mol/L hydrochloric acid-ethyl acetate (6.24 mL) was added thereto. The mixture was stood at room temperature for 4 hr. The precipitated solid was collected by filtration to give the title compound (1.94 g).

¹ H-NMR(DMSO-d₆ )01.72-2.35(5H,m), 2.78-2.99(lH,m), 3.103.30(lH,m), 3.36-3.88(3H,m), 4.47-4.72(2H,m), 4.79-5.13(lH,m), 7.11(lH,d,J=5.1Hz), 8.20(lH,brs), 8.69(lH,d,J=4.2Hz), 8.94(lH,brs), 10.73(1H,brs).

Example 26

Synthesis of (S)-l-[(2S,4S)-4-(2-benzoxazolyl)amino-2pyrrolidinylcarbonyl]-2-cyanopyrrolidine hydrochloride (1) (2S,4S)-4-(2-Benzoxazolyl)amino-l-tertbutoxycarbonylpyrrolidine-2-carboxylic acid (title compound of Reference Example 5, 1.04 g) and (S)-2-cyanopyrrolidine hydrochloride (0.40 g) were dissolved in DMF (5 mL), and triethylamine (0.84 mL), HOBT (0.51 g) and EDC hydrochloride (0.63 g) were successively added thereto. The mixture was stirred at room temperature for 15 hr. Water was added to the reaction mixture, and the mixture was extracted with ethyl

CA 02418656 2003-02-06 acetate. The extract was dried and evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-l-[(2S,4S)-4-(2-benzoxazolylJaminol-tert-butoxycarbonyl-2-pyrrolidinylcarbonyl]-2cyanopyrrolidine (0.86 g) as amorphous.

(2) The above-mentioned compound (0.86 g) was dissolved in 4 mol/L hydrochloric acid-1,4-dioxane (6 mL), and the mixture was stood at room temperature for 15 hr. The precipitated white solid was collected by filtration to give the title compound (0.828 g).

¹H-NMR(DMSO-de)01.70-2.36(5H,m), 2.87-3.50(2H,m), 4.205.15(3H,m), 7.00-7.49(4H,m), 8.24-8.38(lH,m), 8.97(lH,brs), 10.26(1H,brs).

Example 27

Synthesis of (S)-l-[(2S,4R)-4-(2-benzoxazolyl)amino-2pyrrolidinylcarbony1]-2-cyanopyrrolidine hydrochloride (1) (2S,4R)-4-(2-Benzoxazolyl)amino-1-tertbutoxycarbony lpyrrolidine-2-carboxylie acid (title compound of Reference Example 6, 1.0 g) and (S)-2-cyanopyrrolidine hydrochloride (0.38 g) were dissolved in DMF (10 mL), and triethylamine (0.81 mL), HOBT (0.49 g) and EDC hydrochloride (0.61 g) were successively added thereto. The mixture was stirred at room temperature for 15 hr. Water was added to the reaction mixture, and mixture was extracted with ethyl acetate. The extract was dried and the residue was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)—1—[(2S,4R)-4-(2-benzoxazolyl)aminol-tert-butoxycarbonyl-2-pyrrolidinylcarbonyl]-2- cyanopyrrolidine (0.91 g) as amorphous.

(2) The above-mentioned compound (0.91 g) was dissolved in 4 mol/L hydrochloric acid-1,4-dioxane (6 mL), and the mixture was stood at room temperature for 15 hr. The precipitated white solid was collected by filtration to give the title compound

CA 02418656 2003-02-06 (0.841 g).

¹ H-NMR(DMSO-d₆)61.93-2.73(6H,m), 3.30-3.94(4H,m), 4.365.18(3H,m), 6.96-7.50(4H,m), 8.42-9.05(2H,m), 10.17(lH,brs). Example 28

Synthesis of (S)-l-[(2S,4S)-4-(4-chlorophenylmethyl)amino-2pyrrolidinylcarbonyl]-2-cyanopyrrolidine dihydrochloride (1) The title compound (1.54 g) of Reference Example 3 and 4chlorobenzaldehyde (0.703 g) were dissolved in methanol (15 mL), and the mixture was stirred at room temperature for 1 hr.

Sodium cyanoborohydride (0.315 g) and acetic acid (0.4 mL) were added to the reaction mixture, and the mixture was stirred for 1 hr. The reaction mixture was filtrated and the filtrate was evaporated under reduced pressure. Saturated aqueous sodium hydrogencarbonate solution was added to the residue, and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-1-[(2S,4S)-l-tert-butoxycarbonyl-4-(4chlorophenylmethyl)amino-2-pyrrolidinylcarbonyl]-2cyanopyrrolidine (1.67 g) as amorphous.

(2) The above-mentioned compound (0.643 g) was dissolved in ethyl acetate (1.9 mL) and 4 mol/L hydrochloric acid-ethyl acetate (1.9 mL) was added thereto. The mixture was stood at room temperature for 15 hr. The precipitated solid was collected by filtration to give the title compound (0.62 g).

¹ H-NMR(DMSO-de)62.00-2.37(5H,m), 2.90-3.05(lH,m), 3.523.73(4H,m), 3.86-4.04(lH,m), 4.24(2H,s), 4.48-4.54(lH,m), 4.805.17(lH,m), 7.53(2H,d,J-8.4Hz), 7.66(2H,d,J=8.4Hz), 9.07(lH,brs), 10.20(2H,brs), 10.72(1H,brs).

Example 29

Synthesis of (S)-2-cyano-l-[(2S,4S)-4-(4nitrophenylmethyl)amino-2-pyrrolidinylcarbony1]pyrrolidine dihydrochloride

CA 02418656 2003-02-06 (1) The title compound (1.54 g) of Reference Example 3 and 4nitrobenzaldehyde (6.801 g) were dissolved in methanol (15 mL), and the mixture was stirred at room temperature for 1 hr.

Sodium cyanoborohydride (0.315 g) and acetic acid (0.4 mL) were added to the reaction mixture, and the mixture was stirred for 1 hr. The reaction mixture was filtrated and the filtrate was evaporated under reduced pressure. Saturated aqueous sodium hydrogencarbonate solution was added to the residue and the mixture was extracted with chloroform. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-

1- [(2S,4S)-1-tert-butoxycarbony1-4-(4-nitrophenylmethyl)amino-

2- pyrrolidinylcarbonyl]-2-cyanopyrrolidine (1.15 g) as amorphous.

(2) The above-mentioned compound (1.15 g) was dissolved in ethyl acetate (3 mL) and 4 mol/L hydrochloric acid-ethyl acetate (3.3 mL) was added thereto. The mixture was stood at room temperature for 15 hr. The precipitated solid was collected by filtration to give the title compound (0.975 g). ¹H-NMR(DMSO-d₆)01.96-2.37(5H,m), 2.93-3.07(lH,m), 3.663.75(3H,m), 3.93-4.10(lH,m), 4.40(2H,s), 4.50-4.67(lH,m), 4.805.17(lH,m), 7.92(2H,d,J=8.7Hz), 8.31(2H,d,J=8.7Hz),

9.10(1H,brs), 10.10-11.30(2H,m).

Example 30

Synthesis of (S)-2-cyano-l-[(2S,4S)-4-(4phenoxyphenylmethy1)amino-2-pyrrolidinylcarbonyl]pyrrolidine dihydrochloride (1) The title compound (0.924 g) of Reference Example 3 and 4phenoxybenzaldehyde (0.594 g) were dissolved in methanol (15 mL), and the mixture was stirred at room temperature for 1 hr. Sodium cyanoborohydride (0.189 g) and acetic acid (0.2 mL) were added to the reaction mixture, and the mixture was stirred for 1 hr. The reaction mixture was evaporated under reduced

CA 02418656 2003-02-06 pressure. Saturated aqueous sodium hydrogencarbonate solution was added to the residue and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-l-[(2S,4S)-l-tertbutoxycarbonyl-4-(4-phenoxyphenylmethyl)amino-2pyrrolidinylcarbonyl]-2-cyanopyrrolidine (0.99 g) as amorphous. (2) The above-mentioned compound (0.99 g) was dissolved in ethyl acetate (2 mL) and 4 mol/L hydrochloric acid-ethyl acetate (2.5 mL) was added thereto. The mixture was stood at room temperature for 15 hr. The precipitated solid was collected by filtration to give the title compound (0.95 g).

¹ H-NMR(DMSO-de)01.95-2.38(5H,m), 2.90-3.16(lH,m), 3.403.70(4H,m), 3.89-4.03(lH,m), 4.21(2H,s), 4.50-4.67(lH,m), 4.805.17(lH,m), 6.94-7,22(5H,m), 7.34-7.48(2H,m),

7.64(2H,d,J=8.4Hz), 9.10(lH,brs), 10.17(2H,brs), 10.90(lH,brs). Example 31

Synthesis of (S)-2-cyano-l-[(2S,4S)-4-(4cyanophenylmethyl)amino-2-pyrrolidinylcarbonyl]pyrrolidine dihydrochloride (1) The title compound (924 mg) of Reference Example 3 and 4cyanobenzaldehyde (589 mg) were dissolved in methanol (15 mL), and the mixture was stirred at room temperature for 1 hr. Sodium cyanoborohydride (283 mg) and acetic acid (0.4 mL) were added to the reaction mixture, and the mixture was stirred for 1 hr. The reaction mixture was filtrated and the filtrate was evaporated under reduced pressure. Saturated aqueous sodium hydrogencarbonate solution was added to the residue and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-1-[(2S,4S)-1-tert-butoxycarbony1-4-(4cyanophenylmethyl)amino-2-pyrrolidinylcarbonyl]-264

CA 02418656 2003-02-06 cyanopyrrolidine (940 mg) as amorphous.

(2) The above-mentioned compound (940 mg) was dissolved in ethyl acetate (3.0 mL) and 4 mol/L hydrochloric acid-ethyl acetate (2.8 mL) was added thereto. The mixture was stood at room temperature for 15 hr. The precipitated solid was collected by filtration to give the title compound (655 mg). ¹ H-NMR(DMSO-d₆ )01.94-2.37(6H,m), 2.89-3.09(lH,m), 3.273.80(3H,m), 3.90-4.10(lH,m), 4.23-4.44(2H,s), 4.53-4.69(lH,m),

4.80-5.20(lH,m), 7.87(2H,d,J=8.4Hz), 7.94(2H,d,J=8.4Hz), 9.18(lH,brs), 10.60(2H,brs).

Example 32

Synthesis of (S)-l-{(2S,4S)-4-[N-(4-cyanophenylmethyl)-Nmethylamino]-2-pyrrolidinylcarbonyl}-2-cyanopyrrolidine dihydrochloride (1) (S)-l-[(2S,4S)-l-tert-Butoxycarbonyl-4-(4cyanophenylmethyl)amino-2-pyrrolidinylcarbonyl]-2- cyanopyrrolidine [product of Example 31 (1), 1.04 g] and 37% formaldehyde solution (0.7 mL) were dissolved in acetonitrile (15 mL), and the mixture was stirred at room temperature for 1 hr. Sodium cyanoborohydride (0.240 g) and acetic acid (0.4 mL) were added to the reaction mixture, and the mixture was stirred for 1 hr. The reaction mixture was evaporated under reduced pressure. Saturated aqueous sodium hydrogencarbonate solution was added to the residue and the mixture was extracted with chloroform. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-l-{(2S,4S)-l-tertbutoxycarbonyl-4-[N-(4-cyanophenylmethyl)-N-methylamino]-2pyrrolidinylcarbonyl}-2-cyanopyrrolidine (0.71 g) as amorphous.

(2) The above-mentioned compound (0.70 g) was dissolved in ethyl acetate (2.0 mL) and 4 mol/L hydrochloric acid-ethyl acetate (2.0 mL) was added thereto. The mixture was stood at room temperature for 15 hr. The precipitated solid was

CA 02418656 2003-02-06 collected by filtration to give the title compound (0.548 g).

¹ H-NMR(DMSO-d₆ )02.00-2.42(5H,m), 2.59(3H,S), 2.88-3.18(lH,m),

3.50-5.20(9H,m), 7.89(2H,d,J=8.1Hz), 7.97(2H,d,J=8.1Hz), 9.26(lH,brs), 10.96(lH,brs), 12.42(lH,brs).

Example 33

Synthesis of (S)-2-cyano-l-((2S,4S)-4-[N,N-bis(4cyanophenylmethyl) amino]-2-pyrrolidinylcarbonyl}pyrrolidine dihydrochloride (1) The title compound (0.924 g) of Reference Example 3, 4cyanobenzyl bromide (1.27 g) and diisopropylethylamine (1.6 mL) were dissolved in N-methyl-2-pyrrolidone (10 mL), and the mixture was stirred at room temperature for 15 hr. Water was added to the reaction mixture and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-1-((2S,4S)-l-tertbutoxycarbonyl-4- [ N, N-bis (4-cyanophenylmethyl) amino]-2pyrrolidinylcarbonyl}-2-cyanopyrrolidine (1.11 g) as a white solid.

(2) The above-mentioned compound (0.95 g) was dissolved in ethyl acetate (2.2 mL) and 4 mol/L hydrochloric acid-ethyl acetate (2.2 mL) was added thereto. The mixture was stood at room temperature for 15 hr. The precipitated solid was collected by filtration to give the title compound (0.85 g).

¹ H-NMR(DMSO-d6)61.72-2.37(5H,m), 2.62-2.79(lH,m), 3.063.43(2H,m), 3.53-4.50(8H,m), 4.80-5.14(lH,m),

7.55(4H,d,J=8.1Hz), 7.79(4H,d,J=8.1Hz), 8.90(lH,brs),

10.10(lH,brs).

Example 34

Synthesis of (S)-2-cyano-l-[(2S,4S)-4-(3-pyridylmethyl)amino-2pyrrolidinylcarbonyl]pyrrolidine 3 trifluoroacetate (1) The title compound (928 mg) of Reference Example 3 and nicotinaldehyde (321 mg) were dissolved in methanol (16 mL),

CA 02418656 2003-02-06 and the mixture was stirred at room temperature for 30 min. Sodium cyanoborohydride (189 mg) and several drops of acetic acid were added to the reaction mixture, and the mixture was stirred at room temperature for 18 hr. The reaction mixture was filtrated and the filtrate was evaporated under reduced pressure. Saturated aqueous sodium hydrogencarbonate solution was added to the residue and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-l-[(2S,4S)-l-tertbutoxycarbony1-4-(3-pyridylmethyl)amino-2pyrrolidinylcarbonyl]-2-cyanopyrrolidine (591 mg).

(2) The above-mentioned compound (557 mg) was dissolved in dichloromethane (14 mL) and trifluoroacetic acid (1.4 mL) was added thereto. The mixture was stood at room temperature for 18 hr. The reaction solvent was concentrated under reduced pressure, and diethyl ether was added to the residue. The precipitated solid was collected by filtration to give the title compound (704 mg).

¹ H-NMR(DMSO-d₆ )01.70-2.40(5H,m), 2.91-3.14(lH,m), 3.293.73(5H,m), 4.31(2H,s), 4.52-4.70(lH,m), 4.78-5.15(lH,m), 7.58(lH,dd,J=7.8,5.0Hz), 8.02(lH,d,J=7.9Hz), 8.68(lH,dd,J=4.9,1.4Hz), 8.75(lH,d,J=1.7Hz).

Example 35

Synthesis of (S)-2-cyano-l-((2S,4S)-4-phenethylamino-2pyrrolidinylcarbonyl)pyrrolidine 2 trifluoroacetate (1) The title compound (462 mg) of Reference Example 3 and phenylacetaldehyde (0.18 mL) were dissolved in methanol (15 mL), and the mixture was stirred at room temperature for 1 hr. Sodium cyanoborohydride (94 mg) and acetic acid (0.1 mL) were added to the reaction mixture, and the mixture was stirred for 15 hr. The reaction mixture was filtrated and the filtrate was evaporated under reduced pressure. Saturated aqueous sodium

CA 02418656 2003-02-06 hydrogencarbonate solution was added to the residue and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-1-((2S,4S)-l-tert-butoxycarbonyl-4-phenethylamino-2pyrrolidinylcarbonyl)-2-cyanopyrrolidine (300 mg) as amorphous. (2) The above-mentioned compound (300 mg) was dissolved in dichloromethane (7.3 mL) and trifluoroacetic acid (0.73 mL) was added thereto. The mixture was stood at room temperature for 15 hr. The solvent was evaporated under reduced pressure, and the residue was purified by HPLC and freeze-dried to give the title compound (150 mg) as amorphous.

¹ H-NMR(DMSO-d₆ )01.85-2.38(5H,m), 2.83-3.08(3H,m), 3.153.74(4H,m), 3.75-5.18(5H,m), 7.17-7.40(5H,m).

Example 36

Synthesis of (S)-2-cyano-l-((2S,4S)-4-cyclohexylamino-2pyrrolidinylcarbonyl)pyrrolidine dihydrochloride (1) The title compound (924 mg) of Reference Example 3 and cyclohexanone (0.34 mL) were dissolved in methanol (15 mL), and the mixture was stirred at room temperature for 1 hr. Sodium cyanoborohydride (200 mg) and acetic acid (0.4 mL) were added to the reaction mixture, and the mixture was stirred for 15 hr. The reaction mixture was evaporated under reduced pressure. Saturated aqueous sodium hydrogencarbonate solution was added to the residue and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-l-(2S,4S)-l-tert-butoxycarbonyl-4cyclohexylamino-2-pyrrolidinylcarbonyl)-2-cyanopyrrolidine (440 mg) as amorphous.

(2) The above-mentioned compound (430 mg) was dissolved in ethyl acetate (1.5 mL) and 4 mol/L hydrochloric acid-ethyl acetate (1.4 mL) was added thereto. The mixture was stood at

CA 02418656 2003-02-06 room temperature for 15 hr. The precipitated solid was collected by filtration to give the title compound (338 mg).

¹ H-NMR(DMSO-de)01.01-1.47(5H,m), 1.54-2.38(10H,m), 2.883.07(2H,m), 3.38-3.70(4H,m), 3.95-4.13(lH,m), 4.40-4.61(lH,m),

4.80-5.14(lH,m), 9.68(lH,brs), 9.89(lH,brs).

Example 37

Synthesis of (S)-2-cyano-l-((2S,4S)-4-diethylamino-2pyrrolidinylcarbonyl)pyrrolidine dihydrochloride (1) The title compound (924 mg) of Reference Example 3 and 90% aqueous acetaldehyde solution (1.7 mL) were dissolved in methanol (15 mL), and the mixture was stirred at room temperature for 3 hr. Sodium cyanoborohydride (440 mg) and acetic acid (0.4 mL) were added to the reaction mixture, and the mixture was stirred for 1 hr. The reaction mixture was evaporated under reduced pressure. Saturated aqueous sodium hydrogencarbonate solution was added to the residue and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-1-((2S,4S)-l-tert-butoxycarbonyl-4-diethylamino-2pyrrolidinylcarbonyl)-2-cyanopyrrolidine (720 mg) as amorphous.

(2) The above-mentioned compound (720 mg) was dissolved in ethyl acetate (2 mL) and 4 mol/L hydrochloric acid-ethyl acetate (2.5 mL) was added thereto. The mixture was stood at room temperature for 15 hr. The precipitated solid was collected by filtration to give the title compound (713 mg).

¹ H-NMR(DMSO-ds)ôl.20-1.30(6H,m), 1.94-2.36(lH,m), 2.873.38(6H,m), 3.52-4.18(8H,m), 4.45-4.64(lH,m), 4.82-4.90(lH,m), 9.38(lH,brs), 11.30(lH,brs), 11.82(lH,brs).

Example 38

Synthesis of (S)-2-cyano-l-[(2S,4S)-4-(1-piperidino)-2pyrrolidinylcarbonyl]pyrrolidine dihydrochloride (1) The title compound (924 mg) of Reference Example 3 and 50%

CA 02418656 2003-02-06 aqueous glutaraldehyde solution (0.8 mL) were dissolved in methanol (15 mL), and the mixture was stirred at room temperature for 3 hr. Sodium cyanoborohydride (630 mg) and acetic acid (0.4 mL) were added the reaction mixture and the mixture was stirred for 1 hr. The reaction mixture was evaporated under reduced pressure. Saturated aqueous sodium hydrogencarbonate solution was added to the residue and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-1-[(2S,4S)-l-tert-butoxycarbonyl-4-(1-piperidino)-2pyrrolidinylcarbonyl]-2-cyanopyrrolidine (480 mg) as amorphous. (2) The above-mentioned compound (470 mg) was dissolved in ethyl acetate (2 mL) and 4 mol/L hydrochloric acid-ethyl acetate (1.6 mL) was added thereto. The mixture was stood at room temperature for 15 hr. The precipitated solid was collected by filtration to give the title compound (485 mg).

¹ H-NMR(DMSO-de)01.30-1.50(lH,m), 1.61-1.90(5H,m), 1.992.32(3H,m), 2.83-3.09(2H,m), 3.31-3.80(8H,m), 3.90-4.07(lH,m),

4.40-4.62(lH,m), 4,80-5.20(lH,m), 9.27(lH,brs), 11.10(lH,brs), 11.64(lH,brs).

Example 39

Synthesis of (S)-2-cyano-l-{(2S,4S)-4-[N,Nbis(ethoxycarbonylmethyl) amino]-2pyrrolidinylcarbonyl}pyrrolidine dihydrochloride (1) The title compound (0.924 g) of Reference Example 3, ethyl bromoacetate (0.73 mL) and diisopropylethylamine (1.6 mL) were dissolved in N-methy1-2-pyrrolidone (10 mL), and the mixture was stirred at room temperature for 15 hr. Water was added to the reaction mixture and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)—!—{(2S,4S)-1-tert-butoxycarbony1-470

CA 02418656 2003-02-06 [N,N-bis(ethoxycarbonylmethyl) amino]-2-pyrrolidinylcarbonyl}-2cyanopyrrolidine (0.95 g) as a white solid.

(2) The above-mentioned compound (0.80 g) was dissolved in ethyl acetate (2 mL) and 4 mol/L hydrochloric acid-ethyl acetate (2 mL) was added thereto. The mixture was stood at room temperature for 15 hr. The precipitated solid was collected by filtration to give the title compound (0.65 g).

¹ H-NMR ( DMSO-dg)01.19(6H,t,J=7.2Hz), 1.57-1.74(lH,m), 1.932.34(4H,m), 2.96-3.42(2H,m), 3.75-4.50(12H,m), 4.78-5.12(lH,m), 8.80(lH,brs), 10.19(lH,brs).

Example 40

Synthesis of (S)-1-((2S,4S)-4-benzoylamino-2pyrrolidinylcarbony1)-2-cyanopyrrolidine hydrochloride (1) (2S,4S)-4-Benzoylamino-l-tert-butoxycarbonylpyrrolidine-2carboxylic acid (title compound of Reference Example 7, 1.1 g) and (S)-2-cyanopyrrolidine hydrochloride (0.44 g) were dissolved in DMF (10 mL), and triethylamine (0.98 mL), HOBT (0.54 g) and EDC hydrochloride (0.67 g) were successively added. The mixture was stirred at room temperature for 15 hr. Water was added to the reaction mixture and the mixture was extracted with ethyl acetate. The extract was dried and evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-1-((2S,4R)-4-benzoylamino-l-tertbutoxycarbonyl-2-pyrrolidinylcarbonyl)-2-cyanopyrrolidine (1.0 g) as a white solid.

(2) The above-mentioned compound (0.91 g) was dissolved in tetrahydrofuran (3 mL) and 4 mol/L hydrochloric acid-1,4dioxane (6 mL) was added thereto. The mixture was stood at room temperature for 15 hr. The precipitated white solid was collected by filtration to give the title compound (541 mg).

¹ H-NMR(DMSO-de)01.63-2.36(5H,m), 2.75-2.93((lH,m), 3.213.80(4H,m), 4.51-5.13(3H,m), 7.40-7.90(2H,m), 8.52-9.00(2H,m), 10.06(1H,brs).

CA 02418656 2003-02-06

Example 41

Synthesis of (S)-l-((2S,4R)-4-benzoylamino-2pyrrolidinylcarbony1)-2-cyanopyrrolidine hydrochloride (1) ( 2S,4R)-4-Benzoylamino-l-tert-butoxycarbonylpyrrolidine-2carboxylic acid (title compound of Reference Example 8, 1.6 g) and (S)-2-cyanopyrrolidine hydrochloride (0.63 g) were dissolved in DMF (10 mL), and triethylamine (1.32 mL), HOBT (0.79 g) and EDC hydrochloride (0.99 g) were successively added. The mixture was stirred at room temperature for 15 hr. Water was added to the reaction mixture and the mixture was extracted with ethyl acetate. The extract was dried and evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)—1—((2S,4R)-4-benzoylamino-l-tertbutoxycarbonyl-2-pyrrolidinylcarbonyl)-2-cyanopyrrolidine (1.2 g) as a white solid.

(2) The above-mentioned compound (0.97 g) was dissolved in 4 mol/L hydrochloric acid-1,4-dioxane (6 mL). The mixture was stood at room temperature for 15 hr. The precipitated white solid was collected by filtration to give the title compound (0.814 g).

¹ H-NMR(DMSO-d₆ )01.96-2.65(6H,m), 3.23-3.71(4H,m), 4.425.15(3H,m), 7.51-7.97(5H,m), 8.67-9.03(2H,m), 9.92(lH,brs).

Example 42

Synthesis of (S)-1-[(2S,4S)-4-(4-chlorobenzoyl)amino-2pyrrolidinylcarbony1]-2-cyanopyrrolidine hydrochloride (1) The title compound (462 mg) of Reference Example 3 and triethylamine (0.42 mL) were dissolved in dichloromethane (30 mL), and 4-chlorobenzoyl chloride (0.19 mL) was added under ice-cooling. The mixture was stirred at room temperature for 24 hr. Water was added to the reaction mixture and the mixture was extracted with dichloromethane. The extract was dried and evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-l-[(2S,4S)-l-tert72

CA 02418656 2003-02-06 butoxycarbonyl-4-(4-chlorobenzoyl)amino-2pyrrolidinylcarbonyl]-2-cyanopyrrolidine (617 mg) as amorphous.

(2) The above-mentioned compound (610 mg) was dissolved in tetrahydrofuran (4 mL) and 4 mol/L hydrochloric acid-1,4dioxane (3.4 mL) was added thereto. The mixture was stirred at room temperature for 24 hr. The solvent was evaporated under reduced pressure, and the concentrate was purified by HPLC and freeze-dried to give the title compound (340 mg) as amorphous.

¹ H-NMR(DMSO-de)01.87-2.34(5H,m), 2.78-2.93(lH,m), 3.203.75(4H,m), 4.54-4.72(2H,m), 4.85(lH,dd,J=7.8,4.7Hz), 7.58(2H,d,J=8.5Hz), 7.86(2H,d,J=8.5Hz), 8.70(lH,d,J=6.8Hz), 8.88(lH,brs), 9.74(lH,brs).

Example 43

Synthesis of (S)-2-cyano-l-[(2S,4S)-4-(4trifluoromethylbenzoyl)amino-2-pyrrolidinylcarbonyl]pyrrolidine hydrochloride (1) The title compound (462 mg) of Reference Example 3, 4trifluoromethylbenzoic acid (285 mg) and triethylamine (0.42 mL) were dissolved in DMF (30 mL), and HOBT (241 mg) and EDC hydrochloride (302 mg) were successively added under icecooling. The mixture was stirred at room temperature for 22 hr. The solvent was evaporated under reduced pressure, and saturated aqueous sodium hydrogencarbonate solution was added to the concentrate. The mixture was extracted with ethyl acetate. The extract was washed with brine, dried and evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)—1—[(2S,4S)-l-tertbutoxycarbonyl-4- (4-trifluoromethylbenzoyl)amino-2pyrrolidinylcarbonyl]-2-cyanopyrrolidine (640 mg) as a colorless oil.

(2) The above-mentioned compound (640 mg) was dissolved in tetrahydrofuran (15 mL) and 4 mol/L hydrochloric acid-1,4dioxane (3.3 mL) was added thereto. The mixture was stirred at

CA 02418656 2003-02-06 room temperature for 24 hr. The solvent was evaporated under reduced pressure and the concentrate was purified by HPLC and freeze-dried to give the title compound (163 mg) as amorphous.

¹H-NMR(DMSO-d₆)61.92-2.37(5H,m), 2.82-2.96(lH,m), 3.243.65(4H,m), 4.67-4.76(2H,m),

4.89(lH,dd,J=7.8,4.7),7.90(2H,d,J=8.2Hz), 8.06(2H,d,J=8.2Hz), 8.90(lH,d,J=6.7Hz), 9.0-10.0(2H,m).

Example 44

Synthesis of (S)-2-cyano-l-[(2S,4S)-4-(4-cyanobenzoyl)amino-2pyrrolidinylcarbonyl]pyrrolidine hydrochloride (1) The title compound (462 mg) of Reference Example 3, 4cyanobenzoic acid (221 mg) and triethylamine (0.42 mL) were dissolved in DMF (30 mL), HOBT (241 mg) and EDC hydrochloride (302 mg) were successively added under ice-cooling. The mixture was stirred at room temperature for 21 hr. The solvent was evaporated under reduced pressure, and saturated aqueous sodium hydrogencarbonate solution was added to the concentrate. The mixture was extracted with ethyl acetate. The extract was washed with brine, dried and evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-1-[(2S,4S)-1-tert-butoxycarbonyl-4-(4-cyanobenzoyl)amino-2pyrrolidinylcarbonyl]-2-cyanopyrrolidine (548 mg) as amorphous.

(2) The above-mentioned compound (548 mg) was dissolved in tetrahydrofuran (15 mL) and 4 mol/L hydrochloric acid-1,4dioxane (3.1 mL) was added thereto. The mixture was stirred at room temperature for 5 days. The solvent was evaporated under reduced pressure, and the concentrate was purified by HPLC and freeze-dried to give the title compound (177 mg) as amorphous.

¹H-NMR(DMSO-d₆)01.93-2.36(5H,m), 2.80-2.96(lH,m), 3.263.68(4H,m), 4.58-4.76(2H,m), 4.88(lH,dd,J=7.7,4.8Hz), 8.00(4H,s), 8.93(lH,d,J=6.6Hz), 9.0-10.0(2H,m).

Example 45

Synthesis of (S)-2-cyano-l-((2S,4S)-4-nicotinoylamino-274

CA 02418656 2003-02-06 pyrrolidinylcarbonyl)pyrrolidine 2 trifluoroacetate (1) The title compound (462 mg) of Reference Example 3 and triethylamine (0.63 mL) were dissolved in tetrahydrofuran (10 mL), and nicotinoyl chloride hydrochloride (285 mg) was added thereto. The mixture was stirred at room temperature for 15 hr. Water was added to the reaction mixture and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-1-((2S,4S)-1tert-butoxycarbonyl-4-nicotinoylamino-2-pyrrolidinylcarbonyl)2-cyanopyrrolidine (190 mg) as amorphous.

(2) The above-mentioned compound (180 mg) was dissolved in dichloromethane (5 mL), and trifluoroacetic acid (0.5 mL) was added thereto. The mixture was stood at room temperature for 15 hr. The solvent was evaporated under reduced pressure, and the residue was purified by HPLC and freeze-dried to give the title compound (96 mg) as amorphous.

¹ H-NMR(DMSO-de)61.86-2.36(5H,m), 2.80-2.97(lH,m), 3.243.68(4H,m), 4.49-5.13(3H,m), 7.58(lH,dd,J=7.8,4.8Hz), 8.24(lH,d,J=7.8Hz), 8.76(lH,d,J=4.8Hz), 8.87(lH,d,J=6.6Hz), 9.03(lH,s), 9.93(lH,brs).

Example 46

Synthesis of (S)-2-cyano-l-((2S,4S)-4-isonicotinoylamino-2pyrrolidinylcarbonyl)pyrrolidine 2 trifluoroacetate (1) The title compound (462 mg) of Reference Example 3 and triethylamine (0.63 mL) were dissolved in tetrahydrofuran (10 mL), and isonicotinoyl chloride hydrochloride (285 mg) was added thereto. The mixture was stirred at room temperature for 15 hr. Water was added to the reaction mixture and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-l((2S,4S)-l-tert-butoxycarbonyl-4-isonicotinoylamino-275

CA 02418656 2003-02-06 pyrrolidinylcarbonyl)-2-cyanopyrrolidine (670 mg) as amorphous. (2) The above-mentioned compound (650 mg) was dissolved in dichloromethane (16 mL) and trifluoroacetic acid (1.6 mL) was added thereto. The mixture was stood at room temperature for 15 hr. The solvent was evaporated under reduced pressure, and the residue was purified by HPLC and freeze-dried to give the title compound (412 mg) as amorphous.

¹H-NMR(DMSO-d₆)01.87-2.36(5H,m), 2.79-2.96(lH,m), 3.203.68(4H,m), 4.48-5.15(3H,m), 7.80(2H,d,J=6.0Hz), 8.709.13(4H,m), 9.93(lH,brs).

Example 47

Synthesis of (S)-2-cyano-l-((2S,4S)-4-glycylamino-2pyrrolidinylcarbonyl)pyrrolidine dihydrochloride (1) The title compound (464 mg) of Reference Example 3 and Nmethylmorpholine (0.16 mL) were dissolved in tetrahydrofuran (10 mL), and isobutyl chloroformate (0.19 mL) was added thereto at -20°C. The mixture was stirred for 30 min, and a solution of N-tert-butoxycarbonylglycine (463 mg) and triethylamine (0.21 mL) in DMF (3 mL) was added thereto at -20°C. The mixture was stirred at room temperature for 20 hr. Saturated aqueous sodium hydrogencarbonate solution was added to the reaction mixture and the mixture was extracted with ethyl acetate. The extract was washed with brine, dried and evaporated under reduced pressure. The residue was purified by silica gel chromatography to give (S)-l-[(2S,4S)-l-tertbutoxycarbonyl-4-(N-tert-butoxycarbonylglycyl)amino-2pyrrolidinylcarbonyl]-2-cyanopyrrolidine (680 mg) as amorphous.

(2) The above-mentioned compound (360 mg) was dissolved in 1,4dioxane (1 mL) and 4 mol/L hydrochloric acid-1,4-dioxane (4 mL) was added thereto. The mixture was stirred at room temperature for 4 hr. The precipitated solid was collected by filtration to give the title compound (262 mg) as a white solid.

¹H-NMR(DMSO-d«)01.7-2.3(5H,m), 2.78(lH,m), 3.5(2H,m), 4.2676

CA 02418656 2003-02-06

4.60(2H,m), 4.84(lH,dd,J=7.9,4.6Hz), 8.24(3H,brs), 8.79.1(2H,m), 10.71(lH,brs).

Example 48

Synthesis of (S)-2-cyano-l-[(2S,4S)-4-(ethoxyoxalyl)amino-2pyrrolidinylcarbonyl]pyrrolidine trifluoroacetate (1) The title compound (464 mg) of Reference Example 3 and triethylamine (0.63 mL) were dissolved in tetrahydrofuran (10 mL), and ethyl chloroglyoxylate (0.18 mL) was added thereto under ice-cooling. The mixture was stirred for 2 hr and the solvent was evaporated under reduced pressure. Saturated aqueous sodium hydrogencarbonate solution was added to the concentrate and the mixture was extracted with ethyl acetate. The extract was washed with brine and dried. The solvent was evaporated under reduced pressure and the residue was purified by silica gel chromatography to give (S)—1—[(2S,4S)-l-tertbutoxycarbonyl-4- ( ethoxyoxalyl)amino-2-pyrrolidinylcarbonyl]-2cyanopyrrolidine (615 mg) as amorphous.

(2) The above-mentioned compound (492 mg) was dissolved in acetonitrile (10 mL), and trifluoroacetic acid (2 mL) was added thereto under ice-cooling. The mixture was stood at room temperature for 28 hr. The solvent was evaporated under reduced pressure, and the residue was purified by HPLC and freeze-dried to give the title compound (168 mg) as amorphous.

¹H-NMR(DMSO-d₆)01.27(3H,t,J=7.1Hz), 1.82-2.35(5H,m), 2.82(lH,m), 3.26(lH,dd,J=ll.5,7.1Hz), 3.45(lH,dd,J=ll.5,7.1Hz), 3.5(2H,m), 4.24(2H,q,J=7.1Hz), 4.43-4.62(2H,m), 4.85(lH,dd,J=7.8,4.8Hz), 8.89(lH,brs), 9.18(lH,d,J=7.8Hz), 9.78(lH,brs).

Example 49

Synthesis of (S)-2-cyano-l-[(2S,4S)-4-(4-pyridyl)oxy-2pyrrolidinylcarbonyl]pyrrolidine dihydrochloride (1) The title compound (464 mg) of Reference Example 2, 4hydroxypyridine (144 mg) and triphenylphosphine (393 mg) were dissolved in tetrahydrofuran (20 mL), and 40% toluene solution

CA 02418656 2003-02-06 (0.71 mL) of diethyl diazodicarboxylate was added thereto. The mixture was stirred for 9 days. The solvent was evaporated under reduced pressure, and the concentrate was purified by HPLC and freeze-dried to give (S)—1—[(2S,4S)-l-tertbutoxycarbonyl-4-(4-pyridyl)oxy-2-pyrrolidinylcarbony1]-2cyanopyrrolidine (178 mg) as amorphous.

(2) The above-mentioned compound (173 mg) was dissolved in ethyl acetate (1.0 mL) and 4 mol/L hydrochloric acid-ethyl acetate (0.6 mL) was added thereto. The mixture was stood at room temperature for 5 hr. The precipitated white solid was collected by filtration to give the title compound (159 mg).

¹ H-NMR(DMSO-d₆)61.73-2.42(5H,m), 2.87-3.03(lH,m), 3.2-3.8(4H,m), 4.66-4.87(2H,m), 5.53-5.67(m,1H),7.52(lH,d,J=7.2Hz), 8.78(lH,d,J=7.2Hz), 8.81(lH,d,J=7.2Hz), 8.96(lH,brs), 10.86(lH,brs).

Example 50

Synthesis of (S)-l-[(2S,4S)-4-(4-aminobenzoyl)oxy-2pyrrolidinylcarbonyl]-2-cyanopyrrolidine dihydrochloride (1) The title compound (619 mg) of Reference Example 1 and triethylamine (0.84 mL) were dissolved in dichloromethane (10 mL), and 4-nitrobenzoyl chloride (557 mg) and 4dimethylaminopyridine (24 mg) were added thereto. The mixture was stirred at room temperature for 14 hr. Saturated aqueous sodium hydrogencarbonate solution was added to the reaction mixture and the mixture was extracted with chloroform. The extract was washed with brine and dried. The solvent was evaporated under reduced pressure, and the residue was purified by silica gel chromatography and recrystallized from ethyl acetate-hexane to give (S)—1—[(2S,4S)-l-tert-butoxycarbonyl-4- (4-nitrobenzoyl)oxy-2-pyrrolidinylcarbony1]-2-cyanopyrrolidine (800 mg) as pale-brown crystals.

(2) The above-mentioned compound (600 mg) was dissolved in ethyl acetate (15 mL), and the mixture was stirred under a

CA 02418656 2003-02-06 hydrogen atomosphere (1 atm) in the presence of 10% palladium/carbon (123 mg) for 3 hr. The reaction mixture was filtrated and the filtrate was concentrated under reduced pressure. The residue was crystallized from ethyl acetatehexane to give (S)-l-[(2S,4S)-4-(4-aminobenzoyl)oxy-l-tertbutoxycarbony1-2-pyrrolidinylcarbonyl]-2-cyanopyrrolidine (96 mg) as white crystals.

(3) The above-mentioned compound (518 mg) was dissolved in ethyl acetate (1.2 mL) and 4 mol/L hydrochloric acid-ethyl acetate (1.5 mL) was added thereto. The mixture was stood at room temperature for 15 hr. The precipitated pale-brown solid was collected by filtration to give the title compound (378 mg). ¹ H-NMR(DMSO-de)61.97-2.38(5H,m), 2.76-2.88(lH,m), 3.403.70(4H,m), 4.63-4.76(lH,m), 4.88(lH,dd,J=7.9,5.3Hz), 5.425.50(m,1H),6.71(lH,d,J=8.7Hz), 7.72(lH,d,J=8.7Hz), 8.88(1H,brs), 10.84(1H,brs).

Example 51

Synthesis of (S)-2-cyano-l-((2S,4S)-4-nicotinoyloxy-2pyrrolidinylcarbonyl)pyrrolidine dihydrochloride (1) The title compound (619 mg) of Reference Example 1 and triethylamine (0.84 mL) were dissolved in dichloromethane (10 mL), and nicotinoyl chloride hydrochloride (534 mg) and 4dimethylaminopyridine (23 mg) were added thereto. The mixture was stirred at room temperature for 15 hr. Saturated aqueous sodium hydrogencarbonate solution was added to the reaction mixture and the mixture was extracted with chloroform. The extract was washed with brine and dried. The solvent was evaporated under reduced pressure and the residue was purified by silica gel chromatography and recrystallized from ethyl acetate-hexane to give (S)-1-((2S,4S)-1-tert-butoxycarbony1-4nicotinoyloxy-2-pyrrolidinylcarbonyl)-2-cyanopyrrolidine (731 mg) as pale-yellow crystals.

(2) The above-mentioned compound (414 mg) was dissolved in

CA 02418656 2003-02-06 ethyl acetate (2 mL) and 4 mol/L hydrochloric acid-ethyl acetate (1.25 mL) was added thereto. The mixture was stood at room temperature for 18 hr. The precipitated white solid was collected by filtration to give the title compound (524 mg).

¹ H-NMR(DMSO-de)61.97-2.45(5H,m), 2.81-2.96(lH,m), 3.453.85(4H,m), 4.67-4.78(lH,m), 4.86(lH,dd,J=7.9,5.0Hz), 5.605.67(m,lH),7.65(lH,dd,J=8.0,5.1Hz), 8.40-8.47(lH,m), 8.88(lH,dd,J=5.0,1.6Hz), 9.02(lH,brs), 9.17(lH,d,J=1.8Hz), 10.94(lH,brs).

Example 52

Synthesis of (S)-2-cyano-l-((2S,4S)-4-isonicotinoyloxy-2pyrrolidinylcarbonyl)pyrrolidine dihydrochloride (1) The title compound (752 mg) of Reference Example 1 and triethylamine (0.84 mL) were dissolved in dichloromethane (10 mL), and isonicotinoyl chloride hydrochloride (712 mg) and 4dimethylaminopyridine (40 mg) were added thereto. The mixture was stirred at room temperature for 22 hr. Saturated aqueous sodium hydrogencarbonate solution was added to the reaction mixture, and the mixture was extracted with chloroform. The extract was washed with brine and dried. The solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography and crystallized from diethyl ether to give (S)-1-((2S,4S)-l-tert-butoxycarbonyl-4isonicotinoyloxy-2-pyrrolidinylcarbonyl)-2-cyanopyrrolidine (576 mg) as white crystals.

(2) The above-mentioned compound (414 mg) was dissolved in ethyl acetate (1.0 mL) and 4 mol/L hydrochloric acid-ethyl acetate (1.25 mL) was added thereto. The mixture was stood at room temperature for 18 hr. The precipitated white solid was collected by filtration to give the title compound (458 mg).

¹ H-NMR(DMSO-de)01.97-2.47(5H,m), 2.79-2.92(lH,m), 3.5-3.8(4H,m),

4.68-4.81(lH,m), 4.87(lH,dd,J=7.9,5.3Hz), 5.605.67(m,lH),7.97(2H,d,J=6.0Hz), 8.83(2H,d,J=6.0Hz), 8.97(lH,brs),

CA 02418656 2003-02-06

10.98(lH,brs).

Example 53

Synthesis of 3-((2S,4S)-4-amino-2-pyrrolidinylcarbonyl)-l,3thiazolidine dihydrochloride

3-((2S,4S)-4-Amino-l-tert-butoxycarbonyl-2pyrrolidinylcarbonyl)-1,3-thiazolidine (title compound of Reference Example 10, 400 mg) was dissolved in ethyl acetate (3.0 mL), and 4 mol/L hydrochloric acid-ethyl acetate (3.75 mL) was added thereto. The mixture was stood at room temperature for 3 hr. The reaction mixture was concentrated under reduced pressure, and the residue was dissolved in ethanol (100 mL). Activated carbon (0.4 g) was added thereto and the activated carbon was filtered off. The filtrate was concentrated and diethyl ether was added thereto. The precipitated solid was collected by filtration to give the title compound (28.8 mg) as a white powder.

¹ H-NMR(DMSO-d₆)01.85-2.06(lH,m), 2.71-2.93(lH,m), 2.993.20(2H,m), 3.40-3.98(5H,m), 4.37-4.78(3H,m), 8.86(5H,brs).

Example 54

Synthesis of 3-[(2S,4S)-4-(4-cyanophenyl)amino-2pyrrolidinylcarbonyl]-1,3-thiazolidine hydrochloride (1) The title compound (1810 mg) of Reference Example 10, diisopropylethylamine (3.14 mL) and 4-fluorobenzonitrile (727 mg) were dissolved in N-methyl-2-pyrrolidone (18 mL), and the mixture was stirred at 100°C for 24 hr. The reaction mixture was poured into saturated aqueous sodium hydrogencarbonate solution, and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give 3-[(2S,4S)-l-tert-butoxycarbonyl-4-(4cyanophenyl)amino-2-pyrrolidinylcarbonyl]-1,3-thiazolidine (170 mg) as a pale-brown oil.

(2) The above-mentioned compound (170 mg) was dissolved in

CA 02418656 2003-02-06 ethyl acetate (0.42 mL) and 4 mol/L hydrochloric acid-ethyl acetate (0.53 mL) was added thereto. The mixture was stirred at room temperature for 18 hr. The precipitated solid was collected by filtration to give the title compound (69.3 mg) as a pale-brown powder.

¹ H-NMR(DMSO-d₆ )01.64-1.80(lH,m), 2.84-3.20(4H,m), 3.453.96(3H,m), 4.15-4.34(lH,m), 4.39-4.78(3H,m),

6.70(2H,d,J=8.8Hz), 6.85-7.01(lH,m), 7.52(lH,d,J=8,7Hz), 9.45(2H,brs).

Example 55

Synthesis of 3-[(2S,4S)-4-(4-nitropheny1)amino-2pyrrolidinylcarbonyl]-1,3-thiazolidine hydrochloride (1) The title compound (904 mg) of Reference Example 10, diisopropylethylamine (1.57 mL) and 4-fluoronitrobenzene (423 mg) were dissolved in N-methyl-2-pyrrolidone (9 mL), and the mixture was stirred at 80°C for 24 hr. The reaction mixture was poured into saturated aqueous sodium hydrogencarbonate solution, and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give 3-[(2S,4S)-l-tert-butoxycarbonyl-4-(4nitrophenyl)amino-2-pyrrolidinylcarbonyl]-1,3-thiazolidine (919 mg) as yellow amorphous.

(2) The above-mentioned compound (795 mg) was dissolved in ethyl acetate (3.8 mL) and 4 mol/L hydrochloric acid-ethyl acetate (2.4 mL) was added thereto. The mixture was stirred at room temperature for 18 hr. The precipitated solid was collected by filtration to give the title compound (647 mg) as a yellow powder.

¹ H-NMR(DMSO-d6 )01.68-1.87(lH,m), 2.88-3.30(4H,m), 3.483.98(3H,m), 4.24-4.80(4H,m), 6.72(2H,d,J=9.3Hz), 7.407.56(lH,m), 8.04(2H,d,J=7.5Hz), 9.51(2H,brs).

Example 56

CA 02418656 2003-02-06

Synthesis of 3-((2S,4S)-4-(4-methanesulfonylphenyl)amino-2pyrrolidinylcarbonyl]-1,3-thiazolidine hydrochloride (1) The title compound (904 mg) of Reference Example 10, diisopropylethylamine (1.57 mL) and 4-fluorophenyl methyl sulfone (523 mg) were dissolved in N-methy1-2-pyrrolidone (9 mL), and the mixture was stirred at 100°C for 18 hr. The reaction mixture was poured into saturated aqueous sodium hydrogencarbonate solution, and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give 3-((2S,4S)-l-tertbutoxycarbonyl-4-(4-methanesulfonylphenyl)amino-2pyrrolidinylcarbonyl]-l,3-thiazolidine (27 mg).

(2) The above-mentioned compound (27 mg) was dissolved in ethyl acetate (0.2 mL) and 4 mol/L hydrochloric acid-ethyl acetate (0.1 mL) was added thereto. The mixture was stirred at room temperature for 18 hr. The precipitated solid was collected by filtration to give the title compound (19.4 mg).

¹ H-NMR(DMSO-de)61.65-1.82(lH,m), 2.89-3.23(7H,m), 3.493.98(3H,m), 4.18-4.78(4H,m), 6.74(2H,d,J=9.0Hz), 6.806.92(lH,m), 6.63(2H,d,J=9.0Hz), 9.30(2H,brs).

Example 57

Synthesis of 3-((2S,4S)-4-(2-cyanophenyl)amino-2pyrrolidinylcarbonyl]-l,3-thiazolidine hydrochloride (1) The title compound (1810 mg) of Reference Example 10, diisopropylethylamine (3.14 mL) and 2-fluorobenzonitrile (727 mg) were dissolved in N-methyl-2-pyrrolidone (18 mL), and the mixture was stirred at 80°C for 32 hr. The reaction mixture was poured into saturated aqueous sodium hydrogencarbonate solution, and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give 3-((2S,4S)-l-tert-butoxycarbonyl-4-(283

CA 02418656 2003-02-06 cyanophenyl)amino-2-pyrrolidinylcarbonyl]-1,3-thiazolidine (88 mg).

(2) The above-mentioned compound (88 mg) was dissolved in ethyl acetate (0.4 mL) and 4 mol/L hydrochloric acid-ethyl acetate (0.3 mL) was added thereto. The mixture was stirred at room temperature for 18 hr. The precipitated solid was collected by filtration to give the title compound (25 mg).

¹ H-NMR(DMSO-d₆)61.74-1.95(lH,m), 2.85-3.16(3H,m), 3.303.92(4H,m), 4.27-4.79(4H,m), 6.15-6.27(lH,m),

6.77(1H,t,J=7.5Hz), 6.87(lH,d,J=8.4Hz), 7.38-7.59(2H,m),

8.90(lH,brs), 10.80(1H,brs).

Example 58

Synthesis of 3-((2S,4S)-4-(5-cyano-2-pyridyl)amino-2pyrrolidinylcarbonyl]-1,3-thiazolidine dihydrochloride (1) The title compound (904 mg) of Reference Example 10, diisopropylethylamine (1.57 mL) and 2-chloro-5-cyanopyridine (416 mg) were dissolved in N-methy1-2-pyrrolidone (9 mL), and the mixture was stirred at 80°C for 18 hr. The reaction mixture was added to saturated aqueous sodium hydrogencarbonate solution, and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give 3-((2S,4S)-l-tert-butoxycarbonyl-4-(5cyano-2-pyridyl) amino-2-pyrrolidinylcarbonyl]-1,3-thiazolidine (807 mg) as white amorphous.

(2) The above-mentioned compound (711 mg) was dissolved in ethyl acetate (1.76 mL) and 4 mol/L hydrochloric acid-ethyl acetate (2.20 mL) was added thereto. The mixture was stood at room temperature for 18 hr. The precipitated solid was collected by filtration to give the title compound (709 mg) as a white powder.

¹ H-NMR(DMSO-d₆ )61.74-1.94(lH,m), 2.78-2.94(lH,m), 2.973.26(3H,m), 3.40-3.77(3H,m), 4.40-4.80(4H,m),

CA 02418656 2003-02-06

6.64(lH,d,J=9.0Hz), 7.77(lH,dd,J=8.7,2.4Hz), 8.08(lH,brs),

8.46(lH,d,J=1.8Hz), 8.86(lH,brs), 10.37(lH,brs).

Example 59

Synthesis of 3-[(2S,4S)-4-(3,4-dicyanophenyl)amino-2pyrrolidinylcarbonyl]-1,3-thiazolidine hydrochloride (1) The title compound (0.904 g) of Reference Example 10, diisopropylethylamine (1.57 mL) and 4-fluorophthalonitrile (0.438 g) were dissolved in N-methyl-2-pyrrolidone (9 mL), and the mixture was stirred at 80°C for 4 hr. The reaction mixture was poured into saturated aqueous sodium hydrogencarbonate solution, and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give 3-[(2S,4S)-l-tert-butoxycarbonyl-4-(3,4dicyanophenyl)amino-2-pyrrolidinylcarbonyl]-1,3-thiazolidine (1.08 g) as white amorphous.

(2) The above-mentioned compound (0.924 g) was dissolved in ethyl acetate (2.16 mL) and 4 mol/L hydrochloric acid-ethyl acetate (2.70 mL) was added thereto. The mixture was stirred at room temperature for 18 hr. The precipitated solid was collected by filtration to give the title compound (0.782 g) as a yellow powder.

¹ H-NMR(DMSO-d«)01.66-1.84(lH,m), 2.90-3.27(4H,m), 3.493.95(3H,m), 4.20-4.40(lH,m), 4.40-5.79(3H,m),

7.00(lH,dd,J=8.7,2-4Hz), 7.22(lH,s), 7.52-7.67(lH,m), 7.76(lH,d,J=9.0Hz).

Example 60

Synthesis of 3-[(2S,4S)-4-(3-chloro-4-cyanophenyl)amino-2pyrrolidinylcarbonyl]-1,3-thiazolidine hydrochloride (1) The title compound (0.904 g) of Reference Example 10, diisopropylethylamine (1.57 mL) and 2-chloro-4fluorobenzonitrile (0.467 g) were dissolved in N-methyl-2pyrrolidone 9 mL), and the mixture was stirred at 80°C for 8 hr.

CA 02418656 2003-02-06

The reaction mixture was poured into saturated aqueous sodium hydrogencarbonate solution, and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give 3-[(2S,4S)-l-tertbutoxycarbonyl-4-(3-chloro-4-cyanophenyl)amino-2pyrrolidinylcarbonyl]-l,3-thiazolidine (0.630 g) as a colorless transparent oil.

(2) The above-mentioned compound (0.630 g) was dissolved in ethyl acetate (2.88 mL) and 4 mol/L hydrochloric acid-ethyl acetate (1.80 mL) was added thereto. The mixture was stirred at room temperature for 18 hr, and the precipitated solid was collected by filtration to give the title compound (0.465 g) as a white powder.

¹ H-NMR(DMSO-d₆)01.65-1.81(lH,m), 2.84-2.99(lH,m), 2.993.22(3H,m), 3.48-3.95(3H,m), 4.16-4.37(lH,m), 4.39-4.78(3H,m), 6.68(lH,dd,J=8.7,2.1Hz), 6.85(lH,d,J=1.8Hz), 7.30-7.45(lH,m), 7.60(lH,d,J=8.7Hz), 9.60(2H,brs).

Example 61

Synthesis of 3-[(2S,4S)-4-(2-benzoxazolyl)amino-2pyrrolidinylcarbonyl]-1,3-thiazolidine hydrochloride (1) The title compound (904 mg) of Reference Example 10, diisopropylethylamine (1.57 mL) and 2-chlorobenzoxazole (461 mg) were dissolved in N-methy1-2-pyrrolidone (9 mL), and the mixture was stirred at room temperature for 1 hr. The reaction mixture was poured into saturated aqueous sodium hydrogencarbonate solution, and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give 3-[(2S,4S)-4-(2benzoxazolyl)amino-1-tert-butoxycarbony1-2pyrrolidinylcarbonyl]-l,3-thiazolidine (975 mg) as white amorphous.

CA 02418656 2003-02-06 (2) The above-mentioned compound {862 mg) was dissolved in ethyl acetate (4.1 mL) and 4 mol/L hydrochloric acid-ethyl acetate (2.6 mL) was added thereto. The mixture was stirred at room temperature for 8 hr. The precipitated solid was collected by filtration to give the title compound (656 mg) as a white powder.

¹ H-NMR(DMSO-de)01.94-2.13(lH,m), 2.85-2.99(lH,m), 3.003.20(2H,m), 3.31-4.00(4H,m), 4.40-4.82(4H,m), 7.07(lH,td,J=7.8,1.2Hz), 7.18(1H,td,J=7.8,1.2Hz), 7.33(lH,d,J=7.2Hz), 7.42(lH,d,J=7.5Hz), 8.61(lH,brs), 8.89(lHbrs), 10.59(lH,brs).

Example 62

Synthesis of 3-((2S,4S)-4-benzylamino-2-pyrrolidinylcarbonyl)-

1,3-thiazolidine dihydrochloride (1) The title compound (904 mg) of Reference Example 10 and benzaldehyde (318 mg) were dissolved in methanol (16 mL), and the mixture was stirred at room temperature for 30 min. Sodium cyanoborohydride (189 mg) and several drops of acetic acid were added to the reaction mixture, and the mixture was stirred for 6 hr. The reaction mixture was evaporated under reduced pressure. Saturated aqueous sodium hydrogencarbonate solution was added to the residue and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give 3-[(2S,4S)-4-benzylamino-ltert-butoxycarbonyl-2-pyrrolidinylcarbonyl]-1,3-thiazolidine (742 mg) as a colorless transparent oil.

(2) The above-mentioned compound (742 mg) was dissolved in ethyl acetate (3.8 mL) and 4 mol/L hydrochloric acid-ethyl acetate (2.4 mL) was added thereto. The mixture was stirred at room temperature for 18 hr and the precipitated solid was collected by filtration to give the title compound (540 mg) as a white powder.

CA 02418656 2003-02-06 ¹ H-NMR(DMSO-de)Ô2.10-2.32(lH,m), 2.86-3.20(3H,m), 3.494.03(5H,m), 4.21(2H,s), 4.39-4.80(3H,m), 7.31-7.52(3H,m), 7.527.72(2H,m), 10.17(4H,brs).

Example 63

Synthesis of 3-[(2S,4S)-4-(4-cyanophenylmethyl)amino-2pyrrolidinylcarbonyl]-1,3-thiazolidine dihydrochloride (1) The title compound (904 mg) of Reference Example 10 and 4cyanobenzaldehyde (393 mg) were dissolved in methanol (16 mL), and the mixture was stirred at room temperature for 30 min. Sodium cyanoborohydride (189 mg) and several drops of acetic acid were added to the reaction mixture, and the mixture was stirred for 18 hr. The reaction mixture was evaporated under reduced pressure. Saturated aqueous sodium hydrogencarbonate solution was added to the residue and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give 3-[(2S,4S)-l-tertbutoxycarbonyl-4- (4-cyanophenylmethyl)amino-2pyrrolidinylcarbonyl]-l,3-thiazolidine (389 mg) as a colorless transparent oil.

(2) The above-mentioned compound (389 mg) was dissolved in ethyl acetate (0.9 mL) and 4 mol/L hydrochloric acid-ethyl acetate (1.2 mL) was added thereto. The mixture was stood at room temperature for 18 hr. The precipitated solid was collected by filtration to give the title compound (286 mg) as a white powder.

¹ H-NMR(DMSO-de)02.06-2.34(lH,m), 2.85-3.01(lH,m), 3.013.20(2H,m), 3.50-4.06(5H,m), 4.30(2H,s), 4.41-4.79(3H,m), 7.80(2H,d,J=8.1Hz), 7.95(2H,d,J=8.4Hz), 9.05(lH,brs), 10.30(3H,brs).

Example 64

Synthesis of 3-{(2S,4S)-4-[N-(4-cyanophenylmethyl)-Nmethylamino]-2-pyrrolidinylcarbonyl}-1,3-thiazolidine

CA 02418656 2003-02-06 dihydrochloride (1) 3-[(2S,4S)-l-tert-Butoxycarbonyl-4-(4cyanophenylmethyl)amino-2-pyrrolidinylcarbonyl]—1,3— thiazolidine [product of Example 63 (1), 1.35 g] and 37% formaldehyde solution (0.788 mL) were dissolved in acetonitrile (20 mL), and the mixture was stirred at room temperature for 30 min. Sodium cyanoborohydride (0.305 g) and several drops of acetic acid were added to the reaction mixture, and the reaction mixture was stirred for 1 hr. The reaction mixture io was evaporated under reduced pressure. Saturated aqueous sodium hydrogencarbonate solution was added to the residue and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give is 3-{(2S,4S)-l-tert-butoxycarbonyl-4-[N-(4-cyanophenylmethyl)-Nmethylamino]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine (0.953 g) as white amorphous.

(2) The above-mentioned compound (0.818 g) was dissolved in ethyl acetate (3.8 mL) and 4 mol/L hydrochloric acid-ethyl acetate (2.4 mL) was added thereto. The mixture was stirred at room temperature for 5 hr. The precipitated solid was collected by filtration to give the title compound (0.683 g) as a white powder.

¹H-NMR(DMSO-d₆)02.20-2.48(lH,m), 2.57(3H,s), 2.80-3.20(3H,m),

3.57-4.17(5H,m), 4.20-4.85(5H,m), 7.88(2H,d,J=7.8Hz),

7.96(2H,d,J=8.4Hz), 9.12(lH,brs), 10.95(1H,brs), 12.50(1H,brs).

Example 65

Synthesis of 3-[(2S,4R)-4-(7-methoxy-2-phenyl-4-quinolyl)oxy-2pyrrolidinylcarbonyl]-1,3-thiazolidine trihydrochloride (1) N-tert-Butoxycarbonyl-L-trans-hydroxyproline (5.67 g) was dissolved in dimethyl sulfoxide (70 mL), and potassium tertbutoxide (6.88 g) was gradually added thereto at room temperature. The mixture was stirred for 1.5 hr. 4-Chloro-7

CA 02418656 2003-02-06 methoxy-2-phenylquinoline (7.28 g) was gradually added to this solution, and the mixture was stirred for 17 hr. Water was added to the reaction mixture and the mixture was washed with ethyl acetate/hexane (1/1). The aqueous layer was adjusted to pH 4 with 1 mol/L hydrochloric acid, and the precipitate was collected by filtration to give (2S,4R)-l-tert-butoxycarbonyl-

4-(7-methoxy-2-phenyl-4-quinolyl)oxy-2-pyrrolidine-2-carboxylic acid (8.00 g) as a white solid.

(2) Using the above-mentioned compound (546 mg) and in the same manner as in Reference Example 9, 3-[(2S,4R)-l-tertbutoxycarbonyl-4-(7-methoxy-2-phenyl-4-quinolyl)oxy-2pyrrolidinylcarbonyl)-1,3-thiazolidine (568 mg) was obtained as a white solid.

(3) Using the above-mentioned compound (554 mg) and in the same manner as in Example 5 (2), the title compound (521 mg) was obtained as a white powder.

¹ H-NMR(500MHz,DMSO-ds)62.40-2.50(lH,m), 2.96-3.12(3H,m), 3.683.97(4H,m), 3.99(3H,s), 4.48-4.94(3H,m), 4.98(lH,brs), 7.43(lH,d,J=8.1Hz), 7.69-7.70(4H,m), 7.91(lH,brs), 8.26(2H,d,J=7.4Hz), 8.57(lH,brs), 9.10(lH,brs), 11.00(lH,brs). Example 66

Synthesis of 3-((2S,4S)-4-benzoyloxy-2-pyrrolidinylcarbonyl)-

1,3-thiazolidine (1) Using the title compound (643 mg) of Reference Example 11 and benzoyl chloride (0.44 mL), and in the same manner as in Example 50 (1), 3-((2S,4S)-4-benzoyloxy-l-tert-butoxycarbonyl2-pyrrolidinylcarbonyl)-1,3-thiazolidine (515 mg) was obtained as an oil.

(2) The above-mentioned compound (413 mg) was dissolved in ethyl acetate (4 mL) and 4 mol/L hydrochloric acid-ethyl acetate (1.3 mL) was added thereto. The mixture was stirred at room temperature for 14 hr and the solvent was evaporated under reduced pressure. The residue was added to aqueous sodium

CA 02418656 2003-02-06 hydrogencarbonate solution and extracted with ethyl acetate.

The extract was washed with brine and dried. The solvent was evaporated under reduced pressure and the residue was purified by silica gel chromatography to give the title compound (315 mg) as an oil.

¹ H-NMR(DMSO-d₆)ôl.83-1.93(lH,m), 2.45-2.57(lH,m),

2.87(lH,dd,J=12.8,4.2Hz), 2.99(lH,t,J=6.3Hz),

3.08(1H,t,J=6.3Hz), 3.19(lH,d,J=12.8Hz), 3.57-3.77(1,5H,m),

3.83- 3.98(1.5H,m), 4.42(0.5H,d,J=9.5Hz), 4.48-4.58(lH,m), 4.72(0.5H,d,J=9.5Hz), 5.28-5.36(lH,m), 7.52(2H,t,J=7.4Hz), 7.65(lH,t,J=7.4Hz), 7.93(2H,d,J=7.4Hz).

Example 67

Synthesis of 3-[(2S,4S)-4-(4-cyanobenzoyl)oxy-2pyrrolidinylcarbonyl] -1,3-thiazolidine (1) Using the title compound (445 mg) of Reference Example 11 and 4-cyanobenzoyl chloride (371 mg), and in the same manner as in Example 50 (1), 3-[(2S,4S)-l-tert-butoxycarbonyl-4-(4cyanobenzoyl)oxy-2-pyrrolidinylcarbonyl]-l,3-thiazolidine (519 mg) was obtained as a pale-brown solid.

(2) Using the above-mentioned compound (386 mg), and in the same manner as in Example 66 (2), the title compound (280 mg) was obtained as a pale-yellow solid.

¹ H-NMR(DMSO-de)01.88-1.97(lH,m), 2.46-2.58(lH,m), 2.88(lH,dd,J=12.9,4.0Hz), 2.99(lH,t,J=6.3Hz),

3.09(lH,t,J=6.3Hz), 3.23(lH,d,J=12.9Hz), 3.57-3.76(1.5H,m),

3.84- 3.99(1.5H,m), 4.42(0.5H,d,J=9.5Hz), 4.48-4.57(lH,m), 4.72(0.5H,d,J=9.5Hz), 5.33-5.38(lH,m), 8.01(2H,d,J=8.3Hz), 8.07(2H,d,J=8.3Hz).

Example 68

Synthesis of 3-((2S,4S)-4-anilino-2-pyrrolidinylcarbonyl)-l,3thiazolidine dihydrochloride (1) The title compound (501 mg) of Reference Example 12, aniline (0.20 mL) and acetic acid (0.10 mL) were dissolved in

CA 02418656 2003-02-06 methanol (10 mL), and the mixture was stirred at room temperature for 1.5 hr. Sodium cyanoborohydride (145 mg) was added to the reaction mixture and the mixture was stirred for 2 hr. The reaction mixture was evaporated under reduced pressure. Saturated aqueous sodium hydrogencarbonate solution was added to the residue and the mixture was extracted with ethyl acetate. The extract was washed with brine and dried. The solvent was evaporated under reduced pressure, and the residue was purified by silica gel chromatography and crystallized from diethyl ether to give 3-((2S,4S)-4-anilino-l-tert-butoxycarbonyl-2pyrrolidinylcarbonyl)-1,3-thiazolidine (156 mg) as white crystals.

(2) The above-mentioned compound (142 mg) was dissolved in ethyl acetate (2 mL) and 4 mol/L hydrochloric acid-ethyl acetate (0.5 mL) was added thereto. The mixture was stirred at room temperature for 12 hr. The precipitated solid was collected by filtration to give the title compound (89 mg) as a white powder.

¹ H-NMR(DMSO-de)61.64-1.78(lH,m), 2.84-2.97(lH,m), 3.003.19(3H,m), 3.43-3.55(lH,m), 3.60-4.20(5H,m), 4.41-4.76(3H,m), 6.56-6.67(3H,m), 7.13(2H,t,J=7.2Hz), 8.79(lH,brs), 10.29(1H,brs).

Example 69

Synthesis of 3-[(2S,4S)-4-(4-aminophenyl)amino-2pyrrolidinylcarbonyl]-1,3-thiazolidine hydrochloride

The title compound (200 mg) of Example 55 was dissolved in ethanol (10 mL), and 4 mol/L hydrochloric acid-1,4-dioxane (0.28 mL) and 10% palladium/carbon (100 mg) were added. The mixture was stirred under a hydrogen atomosphere (1 atm) at room temperature for 18 hr. The reaction mixture was filtrated and the filtrate was concentrated under reduced pressure, and the obtained solid was washed with ethanol to give the title compound (13 mg) as a white powder.

CA 02418656 2003-02-06 ¹ H-NMR(DMSO-de)01.58-1.80(lH,m), 2.83-3.00(lH,m), 3.003.20(3H,m), 3.60-3.90(3H,m), 4.08-4.25(lH,m), 4.39-4.79(3H,m),

6.67(2H,d,J=8.7Hz), 7.15(2H,d,J=8.7Hz), 8.81(lH,brs),

10.00(3H,brs), 10.25(lH,brs).

Example 70

Synthesis of 3-[(2S,4S)-4-(p-anisidino)-2pyrrolidinylcarbonyl]-1,3-thiazolidine dihydrochloride (1) The title compound (450 mg) of Reference Example 12, panisidine (222 mg) and acetic acid (0.09 mL) were dissolved in

1,2-dichloroethane (8 mL), and sodium triacetoxyborohydride (636 mg) was added thereto. The mixture was stirred at room temperature for 3 hr. Saturated aqueous sodium hydrogencarbonate solution was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The extract was washed with brine and dried. The solvent was evaporated under reduced pressure, and the residue was purified by silica gel chromatography to give 3-[(2S,4S)-4-(p-anisidino)-l-tertbutoxycarbonyl-2-pyrrolidinylcarbonyl]-1,3-thiazolidine (515 mg) as a white solid.

(2) The above-mentioned compound (448 mg) was dissolved in ethyl acetate (10 mL) and 4 mol/L hydrochloric acid-ethyl acetate (1.4 mL) was added thereto. The mixture was stirred at room temperature for 13 hr. The precipitated solid was collected by filtration to give the title compound (223 mg) as a white powder.

¹ H-NMR(DMSO-ds)61.77-1.90(lH,m), 2.77-2.89(lH,m), 3.003.14(3H,m), 3.20-4. 20(lH,m), 3.60-4.20(6H,m), 4.40-4.72(3H,m),

6.87(4H,s), 8.84(lH,brs), 10.33(lH,brs).

Example 71

Synthesis of 3-[(2S,4S)-4-(4-chlorophenyl)amino-2pyrrolidinylcarbonyl]-1,3-thiazolidine dihydrochloride (1) Using the title compound (450 mg) of Reference Example 12 and p-chloroaniline (230 mg), and in the same manner as in

CA 02418656 2003-02-06

Example 70 (1), 3-((2S,4S)-l-tert-butoxycarbonyl-4-(4chlorophenyl)amino-2-pyrrolidinylcarbonyl]-1,3-thiazolidine (415 mg) was obtained as an oil.

(2) Using the above-mentioned compound (412 mg), and in the same manner as in Example 70 (2) the title compound (297 mg) was obtained as a white powder.

¹ H-NMR(DMSO-d₆ )01.63-1.78(lH,m), 2.84-2.97(lH,m), 3.003.17(3H,m), 3.5-3.92(3H,m), 4.07-4.18(lH,m), 4.40-4.73(3H,m), 6.62(2H,d,J=8.8Hz), 7.15(2H,d,J=8.8Hz), 8.86(lH,brs), 10.23(1H,brs).

Example 72

Synthesis of 3-[(2S,4S)-4-(2-chloro-4-cyanophenyl)amino-2pyrrolidinylcarbonyl]-1,3-thiazolidine hydrochloride (1) The title compound (904 mg) of Reference Example 10, diisopropylethylamine (1.57 mL) and 3-chloro-4fluorobenzonitrile (467 mg) were dissolved in N-methyl-2pyrrolidone (9 mL), and the mixture was stirred at 80°C for 8 hr. The reaction solution wa^ added to saturated aqueous sodium hydrogencarbonate solution, extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give 3-((2S,4S)-l-tert-butoxycarbonyl-4-(2chloro-4-cyanophenyl)amino-2-pyrrolidinylcarbonyl)-1,3thiazolidine (460 mg) as a white solid.

(2) The above-mentioned compound (395 mg) was dissolved in ethyl acetate (1.8 mL) and 4 mol/L hydrochloric acid-ethyl acetate (1.13 mL) was added thereto. The mixture was stirred at room temperature for 4 hr, and the precipitated solid was collected by filtration to give the title compound (177 mg) as a white powder.

¹ H-NMR(DMSO-de)01.80-1.99(lH,m), 2.82-3.17(3H,m), 3.253.94(4H,m), 4.36-4.54(2H,m), 4.54-4.80(2H,m),

6.42(lH,d,J=7.8Hz), 6.93(lH,d,J=8.7Hz), 7.64(lH,dd,J=8.4,1.8Hz),

CA 02418656 2003-02-06

7.82(d,J=1.8Hz).

Example 73

Synthesis of 3-[(2S,4S)-4-(3-chloro-4-methoxyphenyl)amino-2pyrrolidinylcarbonyl]-1,3-thiazolidine dihydrochloride (1) Using the title compound (450 mg) of Reference Example 12 and 3-chloro-4-methoxyaniline (284 mg), and in the same manner as in Example 70 (1), 3-[(2S,4S)-l-tert-butoxycarbonyl-4-(3chloro-4-methoxyphenyl)amino-2-pyrrolidinylcarbonyl]-l,3thiazolidine (569 mg) was obtained as an oil.

(2) Using the above-mentioned compound (561 mg), and in the same manner as in Example 70 (2), the title compound (429 mg) was obtained as a pale-brown powder.

¹ H-NMR(DMSO-d₆ )01.62-1.76(lH,m), 2.82-2.95(lH,m), 3.003.18(3H,m), 3.5-3.92(6H,m), 4.07-4.18(lH,m), 4.40-4.73(3H,m),

6.61(lH,dd,J=8.8,2.7Hz), 6.75(lH,d,J=2.7Hz), 6.98(lH,d,J=8.8Hz), 8.80(1H,brs), 10.15(1H,brs).

Example 74

Synthesis of 3-[(2S,4S)-4-(3,4-methylenedioxyphenyl)amino-2pyrrolidinylcarbonyl]-1,3-thiazolidine dihydrochloride (1) Using the title compound (450 mg) of Reference Example 12 and 3,4-methylenedioxyaniline (249 mg), and in the same manner as in Example 70 (1), 3-[(2S,4S)-l-tert-butoxycarbonyl-4-(3,4methylenedioxyphenyl)amino-2-pyrrolidinylcarbonyl]-1,3thiazolidine (553 mg) was obtained as a pale-brown-reddish solid.

(2) Using the above-mentioned compound (549 mg), and in the same manner as in Example 70 (2), the title compound (457 mg) was obtained as a pale-brown-reddish powder.

¹ H-NMR(DMSO-d₆ )01.72-1.85(lH,m), 2.820-2.93(lH,m), 3.003.28(3H,m), 3.45-3.57(lH,m), 3.60-3.95(2H,m), 4.08-4.20(lH,m),

4.42-4.75(3H,m), 5.92(2H,s), 6.25-6.32(lH,m), 6.53(lH,s), 6.766.83(lH,m), 8.89(lH,brs), 10.36(lH,brs).

Example 75

CA 02418656 2003-02-06

Synthesis of 3-[(2S,4S)-4-(5-trifluoromethy1-2-pyridyl)amino-2pyrrolidinylcarbonyl]-1,3-thiazolidine dihydrochloride (1) The title compound (904 mg) of Reference Example 10, diisopropylethylamine (1.57 mL) and 2-chloro-5- trifluoromethylpyridine (545 mg) were dissolved in N-methyl-2pyrrolidone (9 mL), and the mixture was stirred at 80°C for 18 hr. The reaction solution was added to saturated aqueous sodium hydrogencarbonate solution, and extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give 3-[(2S,4S)-l-tert-butoxycarbonyl-4-(5trifluoromethy1-2-pyridyl)amino-2-pyrrolidinylcarbonyl]-1,3thiazolidine (143 mg).

(2) The above-mentioned compound (143 mg) was dissolved in ethyl acetate (0.64 mL) and 4 mol/L hydrochloric acid-ethyl acetate (0.32 mL) was added thereto. The mixture was stirred at room temperature for 18 hr. The precipitated solid was collected by filtration to give the title compound (65 mg) as a brown powder.

¹ H-NMR(DMSO-dg)01.71-1.93(lH,m), 2.80-2.98(lH,m), 3.003.28(3H,m), 3.34-3.99(3H,m), 4.40-4.80(4H,m),

6.68(lH,d,J=9.0Hz), 7.74(lH,dd,J=8.7,2.4Hz), 7.83(lH,brs), 8.35(lH,d,J=1.2Hz), 8.91(lH,brs), 10.22(lH,brs).

Exemple 76

Synthesis of 3-[(2S,4S)-4-(6-cyano-5-trifluoromethy1-2pyridyl ) amino-2-pyrrolidinylcarbonyl]-1,3-thiazolidine hydrochlor ide (1) 2-Chloro-5-trifluoromethylpyridine (5 g) was dissolved in chloroform (150 mL) and m-chloroperbenzoic acid (14.3 g) was added thereto. The mixture was stirred at 60°C for 30 hr. Aqueous sodium thiosulfate solution and saturated aqueous sodium hydrogencarbonate solution were added to the reaction mixture, and after stirring, the mixture was extracted with

CA 02418656 2003-02-06 chloroform. The extract was washed successively with saturated aqueous sodium hydrogencarbonate solution and brine, and dried.

The solvent was evaporated under reduced pressure and the residue was purified by silica gel chromatography to give 2chloro-5-trifluoromethylpyridine 1-oxide (0.64 g).

(2) The above-mentioned compound (610 mg) was dissolved in acetonitrile (5 mL), and triethylamine (0.861 mL) and trimethylsilyl cyanide (1.16 mL) were added thereto. The mixture was refluxed for 4 hr. Saturated aqueous sodium hydrogencarbonate solution was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The extract was washed with brine and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give 6-chloro-3-trifluoromethylpyridine-2carbonitrile (401 mg) as a red oil.

(3) The above-mentioned compound (381 mg), the title compound (556 mg) of Reference Example 10 and diisopropylethylamine (0.96 mL) were dissolved in N-methyl-2-pyrrolidone (6 mL), and the mixture was stirred at room temperature for 18 hr. The reaction solution was added to saturated aqueous sodium hydrogencarbonate solution and extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give 3-[(2S,4S)-l-tert-butoxycarbonyl-4-(6cyano-5-trifluoromethyl-2-pyridyl)amino-2pyrrolidinylcarbonyl]-l,3-thiazolidine (509 mg) as a colorless transparent oil.

(4) The above-mentioned compound (481 mg) was dissolved in ethyl acetate (2.04 mL) and 4 mol/L hydrochloric acid-ethyl acetate (1.02 mL) was added thereto. The mixture was stirred at room temperature for 8 hr. The reaction solvent was evaporated under reduced pressure, and the obtained solid was washed with ethyl acetate to give the title compound (272 mg)

CA 02418656 2003-02-06 as a white powder.

¹ H-NMR(DMSO-de)01.71-1.95(lH,m), 2.77-2.98(lH,m), 2.993.27(3H,m), 3.48-3.99(3H,m), 4.40-4.80(4H,m),

6.96(lH,d,J=9.0Hz), 7.93(lH,d,J=9.0Hz), 8.40(lH,t,J=6.0Hz),

9.55(1H,brs).

Example 77

Synthesis of 3-[(2S,4S)-4-(5,6-dicyano-2-pyridyl)amino-2pyrrolidinylcarbonyl] -1,3-thiazolidine hydrochloride (1) 2-Chloropyridine-5-carbonitrile (7.01 g) was dissolved in io acetonitrile (70 mL), and urea hydrogen peroxide addition compound (10 g) was added. Trifluoroacetic anhydride was added dropwise to the reaction mixture under ice-cooling and the mixture was stirred at room temperature for 18 hr. The reaction mixture was added to aqueous sodium thiosulfate is solution and the mixture was extracted with chloroform. The extract was washed successively with saturated aqueous sodium hydrogencarbonate solution and brine and dried. The solvent was evaporated under reduced pressure and the residue was purified by silica gel chromatography to give 2-chloro-520 cyanopyridine 1-oxide (0.779 g) as a white solid.

(2) Using the above-mentioned compound (779 mg), and in the same manner as in Example 76 (2), 6-chloro-2,3-dicyanopyridine (198 mg) was obtained as a brown solid.

(3) Using the above-mentioned compound (196 mg) and the title compound (361 mg) of Reference Example 10, and in the same manner as in Example 76 (3), 3-[(2S,4S)-l-tert-butoxycarbonyl-

4-(5,6-dicyano-2-pyridyl)amino-2-pyrrolidinylcarbonyl]-1,3thiazolidine (338 mg) was obtained as a white solid.

(4) The above-mentioned compound (338 mg) was dissolved in ethyl acetate (1.58 mL) and 4 mol/L hydrochloric acid-ethyl acetate (0.79 mL) was added thereto. The mixture was stirred at room temperature for 6 hr. The precipitated solid was collected by filtration to give the title compound (178 mg) as

CA 02418656 2003-02-06 a pale-yellow powder.

¹ H-NMR(DMSO-dç) δΐ.71-1.98(lH,m), 2.80-3.00(lH,m), 3.003.24(3H,m), 3.49-4.00(3H,m), 4.39-4.90(3H,m),

6.94(lH,d,J=9.0Hz), 7.97(lH,d,J=8.7Hz), 8.70(lH,brs),

9.55(2H,brs).

Example 78

Synthesis of 3-[(2S,4S)-4-(3-cyanophenylmethyl)amino-2pyrrolidinylcarbonyl]-1,3-thiazolidine dihydrochloride (1) Using the title compound (3.62 g) of Reference Example 10 and 3-cyanobenzaldehyde (1.57 g), and in the same manner as in Example 63 (1), 3-[(2S, 4S)-l-tert-butoxycarbonyl-4-(3cyanophenylmethyl)amino-2-pyrrolidinylcarbonyl)-1,3thiazolidine (2.01 g) was obtained.

(2) The above-mentioned compound (313 mg) was dissolved in ethyl acetate (1.50 mL) and 4 mol/L hydrochloric acid-ethyl acetate (0.94 mL) was added thereto. The mixture was stirred at room temperature for 18 hr. The precipitated solid was collected by filtration to give the title compound (217 mg) as a white powder.

¹ H-NMR(DMSO-d₆)02.09-2.30(lH,m), 2.87-3.01(lH,m),

3.07(lH,t,J=6.3Hz), 3.15(lH,t,J=6.0Hz), 3.50-4.05(5H,m), 4.27(2H,s), 4.40-4.78(3H,m), 7.67(1H,t,J=7.8Hz), 7.848.00(2H,m), 8.10(lH,s), 10.28(3H,brs).

Example 79

Synthesis of 3-[(2S,4S)-4-(4-trifluoromethylphenylmethyl)amino2-pyrrolidinylcarbonyl]-1,3-thiazolidine dihydrochloride (1) Using the title compound (1.81 g) of Reference Example 10 and 4-(trifluoromethyl)benzaldehyde (1.05 g), and in the same manner as in Example 63 (1), 3-[(2S,4S)-l-tert-butoxycarbonyl4-(4-trifluoromethylphenylmethyl)amino-2-pyrrolidinylcarbonyl]-

1,3-thiazolidine (1.69 g) was obtained.

(2) The above-mentioned compound (488 mg) was dissolved in ethyl acetate (2.12 mL) and 4 mol/L hydrochloric acid-ethyl

CA 02418656 2003-02-06 acetate (1.33 mL) was added thereto. The mixture was stirred at room temperature for 18 hr and the precipitated solid was collected by filtration to give the title compound (375 mg) as a white powder.

¹ H-NMR(DMSO-de)62.10-2.32(lH,m), 2.86-3.00(lH,m),

2.50(1H,t,J=1.8Hz), 2.51(1H,t,J=1.8Hz), 3.50-4.02(5H,m), 4.32(2H,s), 4.41-4.80(3H,m), 7.78-7.92(4H,m), 10.35(3H,brs). Example 80

Synthesis of 3-{(2S,4S)-4-[bis(4-cyanophenylmethyl)]amino-2pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) The title compound (0.904 g) of Reference Example 10, 4cyanobenzyl bromide (1.29 g) and diisopropylethylamine (1.57 mL) were dissolved in N-methyl-2-pyrrolidone (9 mL), and the mixture was stirred at room temperature for 18 hr. The reaction mixture was added to saturated aqueous sodium hydrogencarbonate solution, and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give 3-{(2S,4S)-l-tertbutoxycarbonyl-4-[bis (4-cyanophenylmethyl)]amino-2pyrrolidinylcarbonyl}-l,3-thiazolidine (1.27 g).

(2) The above-mentioned compound (1.13 g) was dissolved in ethyl acetate (4.24 mL) and 4 mol/L hydrochloric acid-ethyl acetate (2.65 mL) was added thereto. The mixture was stirred at room temperature for 18 hr and the precipitated solid was collected by filtration to give the title compound (444 mg) as a white powder.

¹ H-NMR(DMSO-d₆)61.81-1.94(lH,m), 2.57-2.79(lH,m), 3.003.95(llH,m), 4.39-4.75(3H,m), 7.55(4H,d,J=8.1Hz), 7.79(4H,d,J=8.1Hz), 8.78(lH,brs), 10.19(lH,brs).

Example 81

Synthesis of 3-[(2S,4S)-4-(4-imidazolylmethyl)amino-2pyrrolidinylcarbonyl]-1,3-thiazolidine trihydrochloride

100

CA 02418656 2003-02-06 (1) Using the title compound (904 mg) of Reference Example 10 and 4-imidazolecarboxyaldehyde (288 mg), and in the same manner as in Example 63 (1), 3-[(2S,4S)-l-tert-butoxycarbonyl-4-(4imidazolylmethyl)amino-2-pyrrolidinylcarbony1]-1,3-thiazolidine (221 mg) was obtained.

(2) The above-mentioned compound (221 mg) was dissolved in ethyl acetate (1.16 mL) and 4 mol/L hydrochloric acid-ethyl acetate (0.72 mL) was added thereto. The mixture was stirred at room temperature for 18 hr. The precipitated solid was collected by filtration, and washed with ethanol to give the title compound (7.7 mg) as a white powder.

¹ H-NMR(DMSO-ds)02.04-2.26(lH,m), 2.88-4.10(8H,m), 4.254.80(5H,m), 7.82(1H,S), 9.07(lH,s).

Example 82

Synthesis of 3-{(2S,4S)-4-[N-benzyl-N-(5-cyano-2pyridyl) amino]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine hydrochloride (1) 3-[(2S,4S)-1-tert-Butoxycarbonyl-4-(5-cyano-2- pyridyl)amino-2-pyrrolidinylcarbonyl]-1,3-thiazolidine [product of Example 58(1)] (305 mg) was dissolved in DMF (10 mL), and potassium tert-butoxide (93 mg) was added under ice cooling. After stirring the mixture for 10 min, benzyl bromide (94 μΐ) was added, and the mixture was stirred at room temperature for 3 days. 10% citric acid solution was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The extract was washed successively with saturated aqueous sodium hydrogencarbonate solution and brine, and dried. The solvent was evaporated under reduced pressure and the residue was purified by silica gel chromatography to give 3-{(2S,4S)-4-[Nbenzyl-N-(5-cyano-2-pyridyl)amino]-l-tert-butoxycarbonyl-2pyrrolidinylcarbonyl}-l,3-thiazolidine (202 mg) as a paleyellow solid.

(2) The above-mentioned compound (196 mg) was dissolved in

101

CA 02418656 2003-02-06 ethyl acetate (2 mL) and 4 mol/L hydrochloric acid-ethyl acetate (0.5 mL) was added thereto. The mixture was stirred at room temperature for 17 hr. The precipitated solid was collected by filtration to give the title compound (112 mg) as a pale-yellow powder.

¹H-NMR(DMSO-d₅)01.91-1.99(lH,m), 2.69-2.76(lH,m), 3.003.11(2H,m), 3.28-3.34(lH,m), 3.41-3.48(lH,m), 3.58-3.90(2H,m),

4.41-4.47(lH,m), 4.58-4.81(4H,m), 5.43-5.48(lH,m),

6.65(lH,d,J=9.0Hz), 7.17-7.38(5H,m), 7.88(lH,dd,J=9.0,2.1Hz), 8.54(lH,d,J=2.1Hz), 8.84(lH,brs), 10.21(1H,brs).

Example 83

Synthesis of 3-[(2S,4S)-4-(l-indolyl)-2-pyrrolidinylcarbonyl]-

1,3-thiazolidine hydrochloride (1) The title compound (950 mg) of Reference Example 13 and thiazolidine (0.27 mL) were dissolved in DMF (20 mL), and HOBT (666 mg) and EDC hydrochloride (666 mg) were added successively. The mixture was stirred at room temperature for 4 hr. The reaction mixture was added to saturated aqueous sodium hydrogencarbonate solution, and extracted with ethyl acetate. The extract was washed with brine and dried. The solvent was evaporated under reduced pressure and the residue was purified by silica gel chromatography to give 3-[(2S,4S)-l-tertbutoxycarbonyl-4- ( 1-indolyl)-2-pyrrolidinylcarbonyl]-l,3thiazolidine (978 mg) as a white solid.

(2) Using the above-mentioned compound (665 mg), and in the same manner as in Example 68 (2), the title compound (486 mg) was obtained as a red powder.

¹ H-NMR(DMSO-d₆ )02.14-2.28(lH,m), 2.98-3.18(4H,m), 3.43.97(3H,m), 4.43-4.87(3H,m), 5.38-5.55(lH,m),

6.55(lH,d,J=3.3Hz), 7.07(lH,t,J=7.1Hz), 7.20(lH,d,J=7.1Hz),

7.48-7.63(3H,m), 9.25(lH,brs), 10.45(1H,brs).

Example 84

Synthesis of 3-((2S,4S)-4-(1-indolinyl)-2102

CA 02418656 2003-02-06 pyrrolidinylcarbonyl]-1,3-thiazolidine dihydrochloride (1) Using the title compound (601 mg) of Reference Example 12 and indoline (0.27 mL), and in the same manner as in Example 70 (1) , 3-[(2S,4S)-l-tert-butoxycarbonyl-4-(1-indolinyl)-2- pyrrolidinylcarbonyl]—1,3-thiazolidine (460 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (436 mg), and in the same manner as in Example 70 (2), the title compound (373 mg) was obtained as a white powder.

¹ H-NMR(DMSO-de)01.83-1.97(lH,m), 2.62-2.77(lH,m),

2.88(2H,t,J=8.2Hz), 3.04(lH,t,J=7.0Hz), 3.12(1H,t,J=6.2Hz), 3.20-3.95(6H,m), 4.40-4.78(4H,m), 6.55-6.68(2H,m), 6.987.09(2H,m), 8.84(lH,brs), 10.31(lH,brs).

Example 85

Synthesis of l-[(2S,4S)-4-(1-indolinyl)-2pyrrolidinylcarbonyl]pyrrolidine dihydrochloride (1) Using the title compound (565 mg) of Reference Example 14 and indoline (0.27 mL), and in the same manner as in Example 70 (1) , 1-((2S,4S)-l-tert-butoxycarbonyl-4-(1-indolinyl)-2- pyrrolidinylcarbonyl]pyrrolidine (653 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (648 mg), and in the same manner as in Example 70 (2), the title compound (491 mg) was obtained as a white powder.

¹ H-NMR(DMSO-de)01.75-1.97(5H,m), 2.60-2.72(lH,m),

2.88(2H,t,J=8.2Hz), 3.218-3.60(8H,m), 4.40-4.55(2H,m), 6.566.67(2H,m), 6.98-7.09(2H,m), 8.75(lH,brs), 10.41(1H,brs).

Example 86

Synthesis of 3-((2S,4S)-4-(5-nitro-l-indolinyl)-230 pyrrolidinylcarbonyl]-l,3-thiazolidine hydrochloride (1) Using the title compound (450 mg) of Reference Example 12 and 5-nitroindoline (295 mg), and in the same manner as in Example 70 (1), 3-((2S,4S)-l-tert-butoxycarbonyl-4-(5-nitro-l103

CA 02418656 2003-02-06 indolinyl)-2-pyrrolidinylcarbonyl]-l,3-thiazolidine (153 mg) was obtained as an oil.

(2) Using the above-mentioned compound (153 mg), and in the same manner as in Example 70 (2), the title compound (116 mg) was obtained as a yellow powder.

¹ H-NMR(DMSO-de)01.92-2.06(lH,m), 2.67-2.80(lH,m), 3.003.17(4H,m), 3.27-3. 94(6H,m), 4.42-4.78(4H,m),

6.62(lH,d,J=8.9Hz), 7.87(lH,d,J=2.3Hz), 8.04(lH,dd,J=8.9,2.3Hz), 9.1(lH,brs), 10.2(1H,brs).

Example 87

Synthesis of 3-[(2S,4S)-4-(6-nitro-l-indolinyl)-2pyrrolidinylcarbonyl]-1,3-thiazolidine dihydrochloride (1) Using the title compound (450 mg) of Reference Example 12 and 6-nitroindoline (296 mg), and in the same manner as in Example 70 (1), 3-[(2S,4S)-l-tert-butoxycarbonyl-4-(6-nitro-lindolinyl)-2-pyrrolidinylcarbonyl]-l,3-thiazolidine (188 mg) was obtained as an oil.

(2) Using the above-mentioned compound (188 mg), and in the same manner as in Example 70 (2), the title compound (80 mg) was obtained as a brown-reddish powder.

¹ H-NMR(DMSO-de)61.84-2.00(lH,m), 2.64-2.77(lH,m), 3.003.17(4H,m), 3.22-3. 92(6H,m), 4.41-4.77(4H,m), 7.22-7.32(2H,m), 7.51(lH,dd,J=7.9,1.9Hz), 8.94(lH,brs), 10.12(lH,brs).

Example 88

Synthesis of 3-[(2S,4S)-4-(5-methoxy-l-indolinyl)-2pyrrolidinylcarbonyl]-1,3-thiazolidine dihydrochloride (1) Using the title compound (751 mg) of Reference Example 12 and 5-methoxyindoline (410 mg), and in the same manner as in Example 70 (1), 3-[(2S,4S)-1-tert-butoxycarbony1-4-(5-methoxyl-indolinyl) -2-pyrrolidiny lcarbonyl] -1, 3-thiazolidine (1010 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (326 mg), and in the same manner as in Example 70 (2), the title compound (262 mg)

104

CA 02418656 2003-02-06 was obtained as a white powder.

¹ H-NMR(DMSO-d₆ )δ1.80-1.95(lH,m), 2.62-2.75(lH,m),

2.86(2H,t,J=7.9Hz), 3.04(lH,t,J=7.0Hz), 3.12(lH,t,J=6.2Hz),

3.17-3.52(4H,m), 3.65(3H,s), 3.66-4.08(6H,m), 4.28-4.77(4H,m), 6.54(lH,d,J=8.5Hz), 6.63(lH,dd,J=8.5,2.4Hz), 6.75(lH,d,J=2.4Hz), 8.83(lH,brs), 10.40(lH,brs).

Example 89

Synthesis of 3-[(2S,4S)-4-(5-hydroxy-l-indolinyl)-2pyrrolidinylcarbonyl]-1,3-thiazolidine dihydrochloride (1) Using the title compound (872 mg) of Reference Example 12 and 5-hydroxyindoline (390 mg), and in the same manner as in Example 70 (1), 3-[(2S,4S)-l-tert-butoxycarbonyl-4-(5-hydroxyl-indolinyl )-2-pyrrolidinylcarbonyl]-l, 3-thiazolidine (538 mg) was obtained as a pale-brown solid .

(2) Using the above-mentioned compound (163 mg), and in the same manner as in Example 70 (2), the title compound (101 mg) was obtained as a white powder.

¹ H-NMR(DMSO-de)δΐ.85-2.00(lH,m), 2.63-2.78(lH,m),

2.88(2H,t,J=7.7Hz), 3.04(1H,t,J=6.6Hz), 3.12(1H,t,J=6,2Hz),

3.24-3.95(6H,m), 4.27-4.76(4H,m), 6.51-6.68(3H,m), 8.96(lH,brs), 10.43(lH,brs).

Example 90

Synthesis of 3-[(2S,4S)-4-(5-acetoxy-l-indolinyl)-2pyrrolidinylcarbonyl]-l,3-thiazolidine dihydrochloride (1) Using 3-[(2S,4S)-l-tert-butoxycarbonyl-4-(5-hydroxy-lindolinyl )-2-pyrrolidinylcarbonyl] -1,3-thiazolidine [product of Example 89 (1), 174 mg] and acetyl chloride (50 μΒ), and in the same manner as in Example 50 (1), 3-[(2S,4S)-4-(5-acetoxy-lindolinyl)-l-tert-butoxycarbonyl-2-pyrrolidinylcarbonyl]-l,3thiazolidine (136 mg) was obtained as an oil.

(2) Using the above-mentioned compound (136 mg), and in the same manner as in Example 70 (2), the title compound (77 mg) was obtained as a pale-brown powder.

105

CA 02418656 2003-02-06 ¹ H-NMR(DMSO-d₆)Ô1.83-1.96(lH,m), 2.20(3H,S), 2.63-2.77(lH,m),

2.89(2H,t,J=8.1Hz), 3.05(1H,t,J=6.8Hz), 3.12(1H,t,J=6.2Hz),

3.18-3.53(4H,m), 3.61-3.93(2H,m), 4.36-4.77(4H,m),

6.56(lH,d,J=8.4Hz), 6.76(lH,dd,J=8.4,2.3Hz), 6.83(1H,d,J=2.3Hz),

8.91(lH,brs), 10.19(lH,brs).

Example 91

Synthesis of 3-[(2S,4S)-4-(5-benzoyloxy-l-indolinyl)-2pyrrolidinylcarbonyl]-1,3-thiazolidine dihydrochloride (1) Using the product (199 mg) of Example 89 (1) and benzoyl chloride (83 μΐ·), and in the same manner as in Example 50 (1),

3-[(2S,4S)-4-(5-benzoyloxy-l-indolinyl)-1-tert-butoxycarbonyl2-pyrrolidinylcarbonyl]-l,3-thiazolidine (173 mg) was obtained as a pale-brown solid.

(2) Using the above-mentioned compound (173 mg), and in the same manner as in Example 70 (2), the title compound (116 mg) was obtained as a pale-brown powder.

¹ H-NMR(DMSO-d6 )01.84-1.98(lH,m), 2.66-2.78(lH,m),

2.93(2H,t,J=8.3Hz), 3.05(1H,t,J=6.7Hz), 3.13(lH,t,J=6.2Hz),

3.23-3.56(4H,m), 3.6-3.95(2H,m), 4.42-4.78(4H,m),

6.62(lH,d,J=8.4Hz), 6.93(lH,dd,J=8.4,2.2Hz), 6.99(lH,d,J=2.2Hz), 7.60(2H,t,J=7.5Hz), 7.74(lH,t,J=7.5Hz), 8.10(2H,d,J=7.5Hz), 8.93(lH,brs), 10.37(lH,brs).

Example 92

Synthesis of 3-[(2S,4S)-4-(5-fluoro-l-indolinyl)-2pyrrolidinylcarbonyl]-1,3-thiazolidine dihydrochloride (1) Using the title compound (496 mg) of Reference Example 12 and 5-fluoroindoline (200 mg), and in the same manner as in Example 70 (1), 3-[(2S,4S)-l-tert-butoxycarbonyl-4-(5-fluoro-lindolinyl )-2-pyrrolidinylcarbonyl]-l,3-thiazolidine (492 mg) was obtained as a pale-brown solid .

(2) Using the above-mentioned compound (487 mg), and in the same manner as in Example 70 (2), the title compound (357 mg) was obtained as a white powder.

106

CA 02418656 2003-02-06 ¹ H-NMR(DMSO-d₆ )01.80-1.95(lH,m), 2.62-2.75(lH,m),

2.88(2H,t,J=8.2Hz), 3.04(lH,t,J=7.0Hz), 3.11(lH,t,J=6.2Hz),

3.18-3.52(4H,m), 3.60-3.94(2H,m), 4.35-4.78(4H,m),

6.55(lH,dd,J=8.8,4.3Hz), 6.85(1H,td,J=8.8,2.6Hz),

6.94(lH,dd,J=8.5,2.6Hz), 8.90(lH,brs), 10.44(lH,brs).

Example 93

Synthesis of 3-[(2S,4S)-4-(5-chloro-l-indolinyl)-2pyrrolidinylcarbonyl]-1,3-thiazolidine hydrochloride (1) Using the title compound (665 mg) of Reference Example 12 and 5-chloroindoline (340 mg), and in the same manner as in Example 70 (1), 3-((2S,4S)-l-tert-butoxycarbony1-4-(5-chloro-lindolinyl) -2-pyrrolidinylcarbonyl]-l, 3-thiazolidine (393 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (389 mg), and in the same manner as in Example 70 (2), the title compound (242 mg) was obtained as a white powder.

¹ H-NMR(DMSO-de)01.81-1.95(lH,m), 2.62-2.74(lH,m),

2.90(2H,t,J=8.3Hz), 3.04(1H,t,J=7.1Hz), 3.12(lH,t,J=6.2Hz),

3.18-3.52(4H,m), 3.60-3.94(2H,m), 4.38-4.77(4H,m),

6.57(lH,d,J=8.3Hz), 7.03-7.ll(2H,m), 8.86(lH,brs), 10.38(lH,brs).

Example 94

Synthesis of 3-((2S,4S)-4-(5-bromo-l-indolinyl)-2pyrrolidinylcarbonyl]-l,3-thiazolidine hydrochloride (1) Using the title compound (0.901 g) of Reference Example 12 and 5-bromoindoline (0.713 g), and in the same manner as in Example 70 (1), 3-((2S,4S)-4-(5-bromo-l-indolinyl)-l-tertbutoxycarbonyl-2-pyrrolidinylcarbonyl]—1,3-thiazolidine (1.31 g) was obtained as a white solid.

(2) Using the above-mentioned compound (340 mg), and in the same manner as in Example 70 (2), the title compound (251 mg) was obtained as a pale-red powder.

¹ H-NMR(DMSO-de)01.82-1.96(lH,m), 2.62-2.74(lH,m),

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2.91(2H,t,J=8.3Hz), 3.04(1H,t,J=7.0Hz), 3.12{lH,t,J=6.2Hz),

3.18-3.54(4H,m), 3.62-3.93(2H,m), 4.37-4.77(4H,m),

6.53(lH,d,J=8.1Hz), 7.15-7.24(2H,m), 8.91(lH,brs),

10.27(lH,brs).

Example 95

Synthesis of 3-[(2S,4S)-4-(1,2,3,4-tetrahydro-l-quinolyl)-2pyrrolidinylcarbonyl]-1,3-thiazolidine hydrochloride (1) Using the title compound (450 mg) of Reference Example 12 and 1,2,3,4-tetrahydroquinoline (0.23 mL), and in the same manner as in Example 70 (1), 3-[(2S,4S)-l-tert-butoxycarbonyl-

4-(1,2,3,4-tetrahydro-l-quinolyl)-2-pyrrolidinylcarbonyl]-1,3thiazolidine (100 mg) was obtained as an oil.

(2) Using the above-mentioned compound (100 mg), and in the same manner as in Example 70 (2), the title compound (60 mg) was obtained as a pale-brown-reddish powder.

¹ H-NMR(DMSO-de)61.76-1.97(3H,m), 2.59-2.73(3H,m), 3.023.5(6H,m), 3.62-3.94(2H,m), 4.42-4.86(4H,m), 6.57(lH,d,J=7.2Hz), 6.80(lH,d,J=8.3Hz), 6.92(lH,d,J=7.2Hz), 6.97-7.07(lH,m), 8.84(lH,brs), 10.04(lH,brs).

Example 96

Synthesis of 3-[(2S,4S)-4-(2-isoindolinyl)-2pyrrolidinylcarbonyl]-l,3-thiazolidine 2 trifluoroacetate (1) The title compound (1.49 g) of Reference Example 10 was dissolved in DMF (50 mL), and potassium carbonate (2.04 g) and α,α'-dibromo-o-xylene (1.37 g) were added thereto. The mixture was stirred at room temperature for 18 hr. Water was added to the reaction mixture and the mixture was extracted with chloroform. The extract was washed with brine and dried. The solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give 3-[(2S,4S)-1tert-butoxycarbonyl-4-(2-isoindolinyl)-2-pyrrolidinylcarbonyl]-

1,3-thiazolidine (1.26 g) as a pale-brown solid .

(2) Using the above-mentioned compound (910 mg), and in the

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CA 02418656 2003-02-06 same manner as in Example 1, the title compound (730 mg) was obtained as a brown powder- ¹ H-NMR(DMSO-d₆ )02.05-2.14(lH,m), 2.88-2.96(lH,m), 3.053.17(2H,m), 3.42-4.02(5H,m), 4.44-4.75(7H,m), 7.31-7.37(4H,m). Example 97

Synthesis of 3-((2S,4S)-4-(N-methylanilino)-2pyrrolidinylcarbonyl]-1,3-thiazolidine dihydrochloride (1) Using the title compound (450 mg) of Reference Example 12 and N-methylaniline (0.22 mL), and in the same manner as in Example 70 (1), 3-[(2S,4S)-l-tert-butoxycarbonyl-4-(Nmethylanilino)-2-pyrrolidinylcarbonyl]-1,3-thiazolidine (2 74 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (216 mg), and in the same manner as in Example 70 (2), the title compound (149 mg) was obtained as a white powder.

¹ H-NMR(DMSO-d₆ )01.83-1.98(lH,m), 2.56-2.69(lH,m), 2.82(3H,s), 3.05(lH,t,J=6.9Hz), 3.11(1H,t,J=6.2Hz), 3.20-3.32(lH,m), 3.363.50(1,m), 3.62-4.0(4H,m), 4.43-4.82(4H,m), 6.85(lH,t,J=7.5Hz), 7.02(lH,d,J=7.5Hz), 7.27(1H,t,J=7.5Hz), 8.89(lH,brs),

10.44(1H,brs).

Example 98

Synthesis of 3-{(2S,4S)-4-(N-(5-cyano-2-pyridyl)-Nmethylamino]-2-pyrrolidinylcarbonyl}-1,3-thia zolidine hydrochloride (1) Using the product (313 mg) of Example 58 (1) and methyl iodide 53 μΕ, and in the same manner as in Example 82 (1), 3{(2S,4S)-l-tert-butoxycarbonyl-4-[N-(5-cyano-2-pyridyl)-Nmethylamino]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine (200 mg) was obtained as a white powder.

(2) Using the above-mentioned compound (198 mg), and in the same manner as in Example 5 (2), the title compound (165 mg) was obtained as a white powder.

¹ H-NMR(DMSO-d₆)01.91-1.99(lH,m), 2.62-2.70(lH,m), 2.98(3H,s),

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27103-383

3.00-3.13(2H,m), 3.30-3.34(2H,m), 3.65-3.93(2H,m), 4.454.77(3H,m), 5.51-5.57(lH,m), 6.84(lH,d,J=9.0Hz),

7.94(lH,dd,J=9.0,2.4Hz), 8.52(lH,d,J=2.4Hz), 8.93(1H,brs),

10.22(1H,brs).

Example 99

Synthesis of 3-{(2S,4S)-4-[N-(3-cyanophenylmethyl)-Nmethylamino]-2-pyrrolidinylcarbonyl}-!,3-thiazolidine dihydrochloride (1) Using 3-[(2S,4S)-l-tert-butoxycarbonyl-4-(3- lo cyanophenylmethyl) amino-2-pyrrolidinylcarbonyl] -1,3thiazolidine [product of Example 78 (1), 313 mg], and in the same manner as in Example 64 (1), 3-{(2S,4S)-1-tertbutoxycarbonyl-4-[N-( 3-cyanophenylmethyl) -N-methylamino]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (598 mg) was obtained as an oil.

(2) The above-mentioned compound (571 mg) was dissolved ih ethyl acetate (2.65 mL) and 4 mol/L hydrochloric acid-ethyl acetate (1.66 mL) was added thereto. The mixture was stirred at room temperature for 18 hr. The precipitated solid was collected by filtration to give the title compound (377 mg) as a white powder.

¹ H-NMR(DMSO-d₆ )02.20-2.42(lH,m), 2.57(3H,brs) , 2.80-3.20(3H,m) ,

3.55-4.10{5H,m), 4.20-4.85(5H,m), 7.68(1H,t,J=7.8Hz), 7.888.05(2H,m), 8.14(lH,brs), 9.12(lH,brs), 10.70(1H,brs).

Example 100

Synthesis of 3-{(2S,4S)-4-[N-(4-cyanophenylmethyl)-N-(2propyl) amino]-2-pyrrolidinylcarbonyl}-1,3-thiazolidine dihydrochloride (1) Using the product (833 mg) of Example 63 (1) and acetone, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tertbutoxycarbonyl-4-[N-(4-cyanophenyhnethyl)-N-(2-propyl) amino]-2pyrrolidinylcarbony.l}-l, 3-thiazolidine (818 mg) was obtained.

(2) The above-mentioned compound (792 mg) was dissolved in

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CA 02418656 2003-02-06 ethyl acetate (3.47 mL) and 4 mol/L hydrochloric acid-ethyl acetate (2.16 mL). was added thereto. The mixture was stirred at room temperature for 18 hr and the precipitated solid was collected by filtration to give the title compound (637 mg) as a white powder.

¹ H-NMR(DMSO-de)δθ.90-1.60(6H,m), 1.95-2.45(lH,m), 2.653.20(3H,m), 3.40-4.90(llH,m), 1.50-8.30(4H,m).

Example 101

Synthesis of 3-{(2S,4S)-4-[N-butyl-N-(4cyanophenylmethyl) amino]-2-pyrrolidinylcarbonyl}-l,3thiazolidine dihydrochloride (1) Using the product (833 mg) of Example 63 (1) and nbutyraldehyde (216 mg), and in the same manner as in Example 70 (1) , 3-{(2S,4S)-l-tert-butoxycarbonyl-4-[N-butyl-N-(4cyanophenylmethyl)amino]-2-pyrrolidinylcarbonyl}-1,3thiazolidine (837 mg) was obtained.

(2) The above-mentioned compound (830 mg) was dissolved in ethyl acetate (3.51 mL) and 4 mol/L hydrochloric acid-ethyl acetate (2.20 mL) was added thereto. The mixture was stirred at room temperature for 18 hr and the precipitated solid was collected by filtration to give the title compound (607 mg) as a pale-yellow powder.

¹ H-NMR(DMSO-de)Ô0.80(3H,t,J=7.2Hz), 1.18(2H,quint,J=6.9Hz),

1.30-1.90(2H,m), 2.10-2.50(lH,m), 2.60-3.24(5H,m), 3.544.87(9H,m), 7.60-8.20(4H,m).

Example 102

Synthesis of 3-{(2S,4S)-4-[N-(4-cyanophenylmethyl)-N-(2hydroxyethyl)amino]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) The product (1.67 g) of Example 63 (1) was dissolved in Nmethyl-2-pyrrolidone (12 mL), and 2-bromoethanol (1.42 mL) and diisopropylethylamine (2.09 mL) were added thereto. The mixture was stirred at 80°C for 2 days. The reaction mixture

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CA 02418656 2003-02-06 was added to saturated aqueous sodium hydrogencarbonate solution and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give 3-{(2S,4S)-l-tert-butoxycarbonyl-4-[N(4-cyanophenylmethyl)-N-(2-hydroxyethyl)amino]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (0.480 g).

(2) The above-mentioned compound (480 mg) was dissolved in ethyl acetate (2.08 mL) and 4 mol/L hydrochloric acid-ethyl acetate (1.04 mL) was added thereto. The mixture was stirred at room temperature for 18 hr and the precipitated solid was collected by filtration to give the title compound (351 mg) as a brown powder.

¹ H-NMR(DMSO-de)01.85-1.97(lH,m), 2.02-2.33(2H,m), 2.704.80(14H,m), 7.60-8.00(4H,m), 9.00(lH,brs), 10.50(lH,brs).

Example 103

Synthesis of 3-{(2S,4S)-4-[N-(carboxymethyl)-N-(5-cyano-2pyridyl) amino]-2-pyrrolidinylcarbonyl]-1,3-thiazolidine tr ifluoroacetate (1) Using the product (461 mg) of Example 58 (1) and tert-butyl bromoacetate (202 μΕ), and in the same manner as in Example 82 (1) , 3-{(2S,4S)-l-tert-butoxycarbonyl-4-[N-(tertbutoxycarbonylmethyl)-N-(5-cyano-2-pyridyl)amino]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (344 mg) was obtained as a pale-yellow powder.

(2) Using the above-mentioned compound (340 mg), and by the synthesis in the same manner as in Example 1 and purification by HPLC, the title compound (118 mg) was obtained as a white solid.

¹ H-NMR(500MHz,DMSO-d₆ )01.93-1.98(lH,m), 2.66-2.69(lH,m), 3.043.12(2H,m), 3.27-3.31(lH,m), 3.40-3.45(lH,m), 3.62-3.87(2H,m), 4.32(2H,s), 4.44-4.71(3H,m), 5.23(lH,m), 6.81(lH,d,J=7.3Hz), 7.95(lH,dd,J=7.3,2.3Hz), 8.51(lH,d,J=2.3Hz).

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Example 104

Synthesis of 3-{(2S,4S)-4-[N-(4-cyanophenylmethyl)-N(ethoxycarbonylmethyl) amino]-2-pyrrolidinylcarbonyl}-l,3thiazolidine dihydrochloride (1) The product (0.833 g) of Example 63 (1) was dissolved in Nmethyl-2-pyrrolidone (6 mL), and ethyl bromoacetate (0.333 mL) and diisopropylethylamine (1.05 mL) were added thereto. The mixture was stirred at room temperature for 18 hr. The reaction mixture was added to saturated aqueous sodium hydrogencarbonate solution, and the mixture was extracted with ethyl acetate. The extract was dried, and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give 3-{(2S,4S)-l-tertbutoxycarbonyl-4-[N-(4-cyanophenylmethyl)-N- (ethoxycarbonylmethyl) amino]-2-pyrrolidinylcarbonyl}-1,3thiazolidine (1.01 g) as an oil.

(2) The above-mentioned compound (976 mg) was dissolved in ethyl acetate (3.88 mL) and 4 mol/L hydrochloric acid-ethyl acetate (2.43 mL) was added thereto. The mixture was stirred at room temperature for 18 hr and the precipitated solid was collected by filtration to give the title compound (630 mg) as a white powder.

¹ H-NMR(DMSO-de)01.18-(3H,t,J=7.1Hz), 1.67-1.90(lH,m), 2.562.75(lH,m), 2.94-3.22(3H,m), 3.25-4.00(8H,m),

4.07(2H,q,J=7.1Hz), 4.34-4.78(3H,m), 7.54(2H,d,J=8.2Hz), 7.81(2H,d,J=8.2Hz), 8.80(lH,brs), 10.40(lH,brs).

Example 105

Synthesis of 3-{(2S,4S)-4-[N-(4-cyanophenylmethyl)-N( isopropoxycarbonylmethyl) amino]-2-pyrrolidinylcarbonyl}-1,3thiazolidine dihydrochloride (1) Using the product (0.833 g) of Example 63 (1) and isopropyl bromoacetate (0.259 mL), and in the same manner as in Example 104 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl-4-[N-(4113

CA 02418656 2003-02-06 cyanophenylmethyl)-N-( isopropoxycarbonylmethyl) amino]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (0.967 g) was obtained as an oil.

(2) The above-mentioned compound (966 mg) was dissolved in ethyl acetate (3.74 mL) and 4 mol/L hydrochloric acid-ethyl acetate (1.87 mL) was added thereto. The mixture was stirred at room temperature for 8 hr. The precipitated solid was collected by filtration to give the title compound (641 mg) as a white powder.

¹ H-NMR(DMSO-de)δ1.18(6H,d,J=6.0Hz), 1.65-1.84(lH,m), 2.552.74(lH,m), 2.95-3.16(3H,m), 3.22-3.47(3H,m), 3.50-3.98(5H,m),

4.39-4.80(3H,m), 4.91(1H,quint,J=6.3Hz), 7.53(2H,d,J=8.1Hz), 7.81(2H,d,J=8.4Hz), 8.70(lH,brs), 10.25(1H,brs).

Example 106 is Synthesis of 3-{(2S,4S)-4-[N-(benzyloxycarbonylmethyl)-N-(4cyanophenylmethyl)amino}-2-pyrrolidinylcarbonyl]-1,3thiazolidine dihydrochloride (1) Using the product (0.833 g) of Example 63 (1) and benzyl bromoacetate (0.317 mL), and in the same manner as in Example

2Q 104 (1), 3-{(2S,4S)-4-[N-(benzyloxycarbonylmethyl)-N-(4cyanophenylmethyl) amino]-1-tert-butoxycarbony1-2pyrrolidinylcarbonyl}-l,3-thiazolidine (0.992 g) was obtained as an oil.

(2) The above-mentioned compound (992 mg) was dissolved in ethyl acetate (3.51 mL) and 4 mol/L hydrochloric acid-ethyl acetate (1.76 mL) was added thereto. The mixture was stirred at room temperature for 8 hr and the precipitated solid was collected by filtration to give the title compound (680 mg) as a white powder.

¹ H-NMR(DMSO-de)01.67-1.85(lH,m), 2.53-2.71(lH,m), 2.943.20(3H,m), 3.20-4.00(8H,m), 4.37-4.80(3H,m), 5.11(2H,s), 7.297.45(5H,m), 7.50(2H,d,J=8.1Hz), 7.78(2H,d,J=8.4Hz), 8.75(lH,brs), 10.15(1H,brs).

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Example 107

Synthesis of 3-{(2S,4S)-4-[N-(carboxymethyl)-N-(4cyanophenylmethyl) amino]-2-pyrrolidinylcarbonyl}-1,3thiazolidine 2 trifluoroacetate (1) Using the product (0.833 g) of Example 63 (1) and tertbutyl bromoacetate (0.443 mL), and in the same manner as in Example 104 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl-4-[N-(tertbutoxycarbonylmethyl)-N-(4-cyanophenylmethyl) amino]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (0.990 g) was obtained.

(2) The above-mentioned compound (881 mg) was dissolved in ethyl acetate (3.06 mL) and 4 mol/L hydrochloric acid-ethyl acetate (6.91 mL) was added thereto. The mixture was stirred at room temperature for 3 days. The precipitated solid was purified by HPLC to give the title compound (141 mg) as a white powder.

¹ H-NMR(DMSO-de)01.65-1.84(lH,m), 2.57-2.74(lH,m), 2.963.19(3H,m), 3.22-4.00(8H,m), 4.37-4.72(3H,m),

7.53(2H,d,J=8.4Hz), 7.81(2H,d,J=8.1Hz), 8.77(lH,brs), 9.63(lH,brs).

Example 108

Synthesis of 3-{(2S,4S)-4-[N-(4-carbamoylphenylmethyl)-N(carboxymethyl)amino]-2-pyrrolidinylcarbonyl}-1,3-thiazolidine trifluoroacetate

The title compound (41 mg) was obtained as a pale-yellow powder by purification of Example 107 (2) by HPLC.

¹ H-NMR(DMSO-de)61.67-1.90(lH,m), 2.56-2.79(lH,m), 2.904.20(llH,m), 4.36-4.74(3H,m), 7.34(lH,brs), 7.56(2H,d,J=8.1Hz), 7.84(2H,d,J=8.4Hz), 7.96(lH,brs), 8.80(lH,brs), 9.60(lH,brs).

Example 109

Synthesis of 3-{(2S,4S)-4-[N-(carbamoylmethyl)-N-(4cyanophenylmethyl) amino]-2-pyrrolidinylcarbonyl}-1,3thiazolidine dihydrochloride (1) Using the product (0.833 g) of Example 63 (1) and 2115

CA 02418656 2003-02-06 bromoacetamide (0.276 mL), and in the same manner as in Example

104 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl-4-[N(carbamoylmethyl)-N-(4-cyanophenylmethyl) amino]-2pyrrolidinylcarbonyl}-1,3-thiazolidine (0.599 g) was obtained.

(2) The above-mentioned compound (599 mg) was dissolved in ethyl acetate (2.53 mL) and 4 mol/L hydrochloric acid-ethyl acetate (1.27 mL) was added thereto. The mixture was stirred at room temperature for 6 hr and the precipitated solid was collected by filtration to give the title compound (416 mg) as a white powder.

¹ H-NMR(DMSO-d₆)δΐ.76-1.99(lH,m), 2.62-2.83(lH,m), 2.904.10(llH,m), 4.25-4.80(3H,m), 7.22(lH,brs), 7.44(lH,brs), 7.64(2H,d,J=8.1Hz), 7.84(2H,d,J=8.4Hz), 8.82(lH,brs), 10.35(lH,brs).

Example 110

Synthesis of 3-((2S,4S)-4-benzoylamino-2-pyrrolidinylcarbonyl)-

1,3-thiazolidine (1) Using the title compound (499 mg) of Reference Example 10 and benzoyl chloride (202 gL), and in the same manner as in Reference Example 7, 3-((2S,4S)-4-benzoylamino-l-tertbutoxycarbonyl-2-pyrrolidinylcarbonyl)-1,3-thiazolidine (652 mg) was obtained as a white powder.

(2) Using the above-mentioned compound (648 mg), and in the same manner as in Example 5 (2), the title compound (250 mg) was obtained as a white powder.

¹ H-NMR(500MHz,DMSO-d₆)01.76-1.81(lH,m), 2.33-2.39(lH,m), 2.913.09(5H,m), 3.63-3.95(3H,m), 4.34-4.70(3H,m), 7.44-7.53(3H,m),

7.80-7.82(2H,m), 8.38(lH,brs).

Example 111

Synthesis of 3-[(2S,4S)-4-(4-cyanobenzoyl)amino-2pyrrolidinylcarbonyl]-1,3-thiazolidine hydrochloride (1) Using the title compound (543 mg) of Reference Example 10 and 4-cyanobenzoyl chloride (313 mg), and in the same manner as

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CA 02418656 2003-02-06 in Reference Example 7, 3-((2S,4S)-l-tert-butoxycarbonyl-4-(4cyanobenzoyl)amino-2-pyrrolidinylcarbonyl]-1,3-thiazolidine (804 mg) was obtained as a white powder.

(2) Using the above-mentioned compound (798 mg), and in the same manner as in Example 5 (2), the title compound (513 mg) was obtained as a white powder.

¹ H-NMR(500MHz,DMSO-de)δ2.01-2.06(lH,m), 2.81-2.86(lH,m), 3.033.14(2H,m), 3.36-3.50(2H,m), 3.65-3.94(2H,m), 4.45-4.75(4H,m),

7.98-8.06(4H,m), 8.86(lH,brs), 9.07-9.12(lH,m), 10.49(lH,brs). Example 112

Synthesis of 3-[(2S,4S)-4-(5-chloro-2-nitrobenzoyl)amino-2pyrrolidinylcarbonyl]-1,3-thiazolidine hydrochloride (1) Using the title compound (495 mg) of Reference Example 10 and 5-chloro-2-nitrobenzoic acid (300 mg), and in the same manner as in Reference Example 9, 3-[(2S,4S)-l-tertbutoxycarbonyl-4-(5-chloro-2-nitrobenzoyl)amino-2pyrrolidinylcarbonyl]-l,3-thiazolidine (340 mg) was obtained as a white powder.

(2) Using the above-mentioned compound (338 mg), and in the same manner as in Example 5 (2), the title compound (272 mg) was obtained as a white powder.

¹ H-NMR(DMSO-d₆ )01.88-1.99(lH,m), 2.79-2.88(lH,m), 3.043.15(2H,m), 3.26-3.36(lH,m), 3.47-3.94(3H,m), 4.44-4.75(4H,m),

7.80-7.84(2H,m), 8.11-8.14(lH,m), 9.09-9.12(lH,m), 9.50(2H,brs).

Example 113

Synthesis of 3-[(2S,4S)-4-(2,4-dichlorobenzoyl)amino-2pyrrolidinylcarbonyl]-1,3-thiazolidine hydrochloride (1) Using the title compound (440 mg) of Reference Example 10 and 2,4-dichlorobenzoic acid (254 mg), and in the same manner as in Reference Example 9, 3-[(2S,4S)-l-tert-butoxycarbonyl-4(2,4-dichlorobenzoy1)amino-2-pyrrolidinyIcarbony1]-1,3thiazolidine (356 mg) was obtained as a white powder.

(2) Using the above-mentioned compound (356 mg), and in the

117

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¹ H-NMR(DMSO-d₆ )01.86-1.99(lH,m), 2.79-2.88(lH,m), 3.043.14(2H,m), 3.26-3.31(lH,m), 3.47-3.95(3H,m), 4.43-4.75(4H,m),

7.50-7.56(2H,m), 7.71(lH,s), 8.84-8.88(lH,m), 9.70(2H,brs).

Example 114

Synthesis of 3-{(2S,4S)-4-[(3-nitrophenyl)acetyl]amino-2pyrrolidinylcarbonyl}-l,3-thiazolidine hydrochloride (1) Using the title compound (370 mg) of Reference Example 10 and 3-nitrophenylacetic acid (201 mg), and in the same manner as in Reference Example 9, 3-{(2S,4S)-l-tert-butoxycarbonyl-4[(3-nitrophenyl)acetyl]amino-2-pyrrolidinylcarbonyl}-l,3thiazolidine (516 mg) was obtained as a white powder.

(2) Using the above-mentioned compound (515 mg), and in the same manner as in Example 5 (2), the title compound (427 mg) was obtained as a white powder.

¹ H-NMR(500MHz,DMSO-d₆ )01.86-1.93(lH,m), 2.74-2.78(lH,m), 3.043.14(3H,m), 3.40-3.44(lH,m), 3.63(2H,s), 3.63-3.89(2H,m), 4.364.70(4H,m), 7.60-7.63(lH,m), 7.72(lH,d,J=7.7Hz),

8.12(lH,d,J=8.2Hz), 8.15(1H,S), 8.65-8.68(lH,m).

Example 115

Synthesis of 3-((2S,4S)-4-(trans-3trifluoromethylcinnamoyl)amino-2-pyrrolidinylcarbonyl]-1,3thiazolidine hydrochloride (1) Using the compound (338 mg) of Reference Example 10 and 3trifluoromethylcinnamoyl chloride (201 μΣ), and in the same manner as in Reference Example 7, 3-((2S,4S)-l-tertbutoxycarbonyl-4- (trans-3-trifluoromethylcinnamoyl)amino-2pyrrolidinylcarbonyl]-l,3-thiazolidine (274 mg) was obtained as a white powder.

(2) Using the above-mentioned compound (270 mg), and in the same manner as in Example 5 (2), the title compound (230 mg) was obtained as a white powder.

118

CA 02418656 2003-02-06 ¹ H-NMR(DMSO-d₆)61.78-1.88(lH,m), 2.79-2.87(lH,m), 3.043.22(3H,m), 3.46-3.52(lH,m), 3.64-3.94(2H,m), 4.46-4.75(4H,m),

6.76(lH,d,J=15.9Hz), 7.57(lH,d,J=15.9Hz), 7.64-7.76(2H,m),

7.89-8.03(2H,m), 8.70-8.75(lH,m), 9.60(2H,brs).

Example 116

Synthesis of 3-{(2S,4S)-4-[N-(4-cyanobenzoyl)-N-(4cyanophenylmethyl) amino]-2-pyrrolidinylcarbonyl}-l,3thiazolidine hydrochloride (1) The product (0.833 g) of Example 63 (1) was dissolved in dichloromethane (10 mL), and triethylamine (0.418 mL) and 4cyanobenzoyl chloride (0.331 g) were added thereto. The mixture was stirred at room temperature for 18 hr. The reaction mixture was added to saturated aqueous sodium hydrogencarbonate solution, and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give 3-{(2S,4S)-l-tertbutoxycarbonyl-4-[N-(4-cyanobenzoyl)-N-(4cyanophenylmethyl) amino]-2-pyrrolidinylcarbonyl}-1,3thiazolidine (0.956 g).

(2) The above-mentioned compound (514 mg) was dissolved in ethyl acetate (1.88 mL) and 4 mol/L hydrochloric acid-ethyl acetate (1.18 mL) was added thereto. The mixture was stirred at room temperature for 18 hr and the precipitated solid was collected by filtration to give the title compound (320 g) as a white powder.

¹ H-NMR(DMSO-ds)01.62-2.39(lH,m), 2.45-2.82(lH,m), 2.903.25(2H,m), 3.30-3.95(4H,m), 4.25-5.00(6H,m), 7.30-8.20(8H,m). Example 117

Synthesis of 3-{(2S,4S)-4-[N-acetyl-N-(5-cyano-2pyridyl)amino]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine hydrochloride (1) Using the product (340 mg) of Example 58 (1) and acetyl

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3-{(2S,4S)-4-[N-acetyl-N-(5-cyano-2-pyridyl) amino]-1-tertbutoxycarbonyl-2-pyrrolidinylcarbonyl}-!.,3-thiazolidine ( 188 mg) was obtained.

(2) Using the above-mentioned compound (186 mg), and in the same manner as in Example 82 (2), the title compound (121 mg) was obtained as a white powder.

¹ H-NMR(500MHz,DMSO-d₆)61.90-1.95(lH,m), 1.91(3H,s), 2.752.80(lH,m), 3.03-3.11(2H,m), 3.40-3.44(lH,m), 3.50-3.54(lH,m),

3.59-3.63(lH,m), 3.71-3.85(lH,m), 4.39-4.44(lH,m), 4.564.69(2H,m), 5.04-5.08(lH,m), 7.75(lH,d,J=8.3Hz), 8.50(lH,dd,J=8.3,2.1Hz), 9.01(lH,d,J=2.1Hz).

Example 118

Synthesis of 3-((2S,4S)-4-phthalimido-2-pyrrolidinylcarbonyl)-

1,3-thiazolidine trifluoroacetate (1) The title compound (1.23 g) of Reference Example 10 was suspended in toluene (20 mL), and phthalic anhydride (632 mg) and triethylamine (60 μΣ) were added thereto. The mixture was refluxed for 5 hr. To the reaction mixture was added 10% citric acid solution and the mixture was extracted with ethyl acetate. The extract was washed successively with saturated aqueous sodium hydrogencarbonate solution and brine, and dried. The solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give 3-((2S,4S)-1tert-butoxycarbonyl-4-phthalimido-2-pyrrolidinylcarbonyl)-1,3thiazolidine (1.21 g) as a white solid.

(2) The above-mentioned compound (362 mg) was dissolved in dichloromethane (4 mL) and trifluoroacetic acid (2 mL) was added thereto. The mixture was stirred at room temperature for 20 hr. The solvent was evaporated under reduced pressure to give the title compound (374 mg) as a pale-yellow solid.

¹ H-NMR(500MHz,DMSO-d₆ )02.38-2.44(lH,m), 2.80-2.84(lH,m), 3.073.13(2H,m), 3.50-3.54(lH,m), 3.60-3.89(3H,m), 4.46-4.50(lH,m),

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4.62-4.78(2H,m), 4.97-5.00(lH,m), 7.86-7.90(4H,m), 8.74(lH,brs),

9.90(lH,brs).

Example 119

Synthesis of 3-[(2S,4S)-4-(4-nitrophthalimido)-2pyrrolidinylcarbonyl]-1, 3-thiazolidine trifluoroacetate (1) Using the title compound (2.31 g) of Reference Example 10 and 4-nitrophthalic anhydride (1.16 g ), and in the same manner as in Example 118 (1), 3-((2S,4S)-l-tert-butoxycarbonyl-4-(4nitrophthalimido)-2-pyrrolidinylcarbonyl]-1,3-thiazolidine (1.42 g) was obtained as a white solid.

(2) Using the above-mentioned compound (355 mg), and in the same manner as in Example 118 (2), the title compound (298 mg) was obtained as a white powder.

¹ H-NMR(500MHz,DMSO-dg)02.40-2.45(lH,m), 2.82-2.88(lH,m), 3.063.14(2H,m), 3.52-3.56(lH,m), 3.62-3.89(3H,m), 4.47-4.50(lH,m),

4.62-4.78(2H,m), 5.01-5.07(lH,m), 8.15(lH,d,J=8.2Hz), 8.51(lH,d,J=1.9Hz), 8.65(lH,dd,J=8.2,1.9Hz), 9.24(2H,brs).

Example 120

Synthesis of 3-[(2S,4S)-4-(3-phenylureido)-2pyrrolidinylcarbonyl]-1,3-thiazolidine trifluoroacetate (1) The title compound (401 mg) of Reference Example 10 was dissolved in tetrahydrofuran (10 mL), and phenyl isocyanate (167 mg) was added thereto at room temperature. The mixture was stirred for 18 hr. To the reaction mixture was added 10% citric acid solution and the mixture was extracted with ethyl acetate. The extract was washed successively with saturated aqueous sodium hydrogencarbonate solution and brine, and dried. The solvent was evaporated under reduced pressure to give 3- [(2S,4S)-l-tert-butoxycarbonyl-4-(3-phenylureido)-2pyrrolidinylcarbonyl]-l,3-thiazolidine (560 mg) as a white solid.

(2) The above-mentioned compound (532 mg) was dissolved in trifluoroacetic acid (2 mL) and the mixture was stirred at room

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CA 02418656 2003-02-06 temperature for 3 hr. The solvent was evaporated under reduced pressure to give the title compound (363 mg) as a brown powder.

¹ H-NMR(500MHz,DMSO-d₆)δ1.75-1.80(lH,m), 2.75-2.80(lH,m), 3.043.20(3H,m), 3.43-3.47(lH,m), 3.68-3.89(2H,m), 4.40-4.71(4H,m), 6.72-6.75(lH,m), 6.91(1H,t,J=7.4Hz), 7.21-7.24(2H,m), 7.39(2H,d,J=7.8Hz), 8.85(lH,brs), 8.89-8.90(lH,m), 9.60(lH,brs). Example 121

Synthesis of 3-{(2S,4S)-4-[3-(4-cyanophenyl)ureido]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (1) Using the title compound (640 mg) of Reference Example 10 and 4-cyanophenyl isocyanate (321 mg), and in the same manner as in Example 120 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl-4-[3- (4-cyanophenyl)ureido]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine (992 mg) was obtained as a white powder.

(2) The above-mentioned compound (978 mg) was dissolved in chloroform (5 mL), and then dissolved in trifluoroacetic acid (3 mL), and the mixture was stirred at room temperature for 8 hr. The solvent was evaporated under reduced pressure and saturated aqueous sodium hydrogencarbonate solution was added to the residue. The mixture was extracted with chloroform.

The extract was concentrated under reduced pressure to give the title compound (140 mg) as a white powder.

¹ H-NMR(DMSO-de)61.60-1.68(lH,m), 2.22-2.32(lH,m), 2.672.72(lH,m), 2.91-3.11(4H,m), 3.65-3.93(3H,m), 4.13-4.16(lH,m),

4.43-4.72(2H,m), 6.47(lH,d,J=7.2Hz), 7.56(2H,d,J=8.7Hz), 7.65(2H,d,J=8.7Hz), 9.11(1H,S).

Example 122

Synthesis of 3-((2S,4S)-4-phenylsulfonylamino-2pyrrolidinylcarbony1)-1,3-thiazolidine hydrochloride (1) The title compound (543 mg) of Reference Example 10 was dissolved in dichloromethane (10 mL), and 4-methylmorpholine (240 pL) and benzenesulfonyl chloride (240 pL) were added thereto at room temperature. The mixture was stirred for 17 hr.

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To the reaction mixture was added 10% citric acid solution, and the mixture was extracted with ethyl acetate. The extract was washed successively with saturated aqueous sodium hydrogencarbonate solution and brine, and dried. The solvent was evaporated under reduced pressure to give 3-((2S,4S)-1tert-butoxycarbonyl-4-phenylsulfonylamino-2pyrrolidinylcarbonyl)-1,3-thiazolidine (644 mg) as a white powder.

(2) The above-mentioned compound (634 mg) was dissolved in ethyl acetate (4 mL) and 4 mol/L hydrochloric acid-ethyl acetate (1.8 mL) was added thereto. The mixture was stirred at room temperature for 67 hr. The precipitate was collected by filtration to give the title compound (437 mg) as a pale-yellow powder.

¹ H-NMR(500MHz,DMSO-de)01.68-1.74(lH,m), 2.50-2.58(lH,m), 3.003.08(3H,m), 3.17-3.20(lH,m), 3.53-3.87(3H,m), 4.38-4.63(3H,m),

7.62-7.71(3H,m), 7.84-7.85(2H,m), 8.24-8.27(lH,m), 9.50(2H,brs). Example 123

Synthesis of 3-[(2S,4S)-4-(4-cyanophenylsulfonyl)amino-2pyrrolidinylcarbonyl]-1,3-thiazolidine hydrochloride (1) Using the title compound (1.09 g) of Reference Example 10 and 4-cyanobenzenesulfonyl chloride (0.780 g), and in the same manner as in Example 122 (1), 3-((2S,4S)-l-tert-butoxycarbonyl4-(4-cyanophenylsulfonyl)amino-2-pyrrolidinylcarbonyl]-1,3thiazolidine (1.67 g) was obtained as a white powder.

(2) Using the above-mentioned compound (798 mg), and in the same manner as in Example 122 (2), the title compound (544 mg) was obtained as a pale-yellow powder.

¹ H-NMR(500MHz,DMSO-de)01.68-1.75(lH,rn), 2.53-2.59(lH,m), 3.023.09(3H,m), 3.23-3.28(lH,m), 3.54-3.90(3H,m), 4.40-4.64(3H,m), 8.01(2H,d,J=8.4Hz), 8.13(2H,d,J=8.4Hz), 8.62-8.65(lH,m), 9.93(2H,brs).

Example 124

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Synthesis of 3-{(2S,4S)-4-[N-(4-cyanophenylmethyl)-N-(4cyanophenylsulfonyl)amino]-2-pyrrolidinylcarbonyl}-l,3thiazolidine hydrochloride (1) 3-[(2S,4S)-l-tert-Butoxycarbonyl-4-(4- cyanophenylsulfonyl)amino-2-pyrrolidinylcarbonyl]-1,3thiazolidine [product of Example 123 (1), 856 mg] was dissolved in DMF (20 mL), and potassium carbonate (380 mg) and 4cyanobenzyl bromide (400 mg) were added thereto at room temperature. The mixture was stirred for 4 hr. To the reaction mixture was added 10% citric acid solution, and the precipitate was collected by filtration to give 3-{(2S,4S)-ltert-butoxycarbonyl-4-[N-(4-cyanophenylmethyl)-N-(4cyanophenylsulfonyl)amino]-2-pyrrolidinylcarbonyl}-l,3thiazolidine (900 mg) as a pale-yellow solid.

(2) Using the above-mentioned compound (900 mg), and in the same manner as in Example 122 (2), the title compound (800 mg) was obtained as a pale-yellow powder.

¹ H-NMR(500MHz,DMSO-d₆ )01.54-1.60(lH,m), 2.45-2.50(lH,m), 2.79(lH,dd,J=8.7,1.6Hz), 3.00-3.20(3H,m), 3.55-3.58(lH,m),

3.68-3.82(lH,m), 4.34-4.63(3H,m), 4.65(2H,s), 4.87-4.93(lH,m), 7.51(2H,d,J=8.2Hz), 7.86(2H,d,J=8.2Hz), 8.11(2H,d,J=8.4Hz), 8.16(2H,d,J=8.4Hz), 8.85(lH,brs), 9.91(lH,brs).

Example 125

Synthesis of 3-[(2S,4S)-4-(1-pyrrolidinyl)-2pyrrolidinylcarbonyl]-1,3-thiazolidine dihydrochloride (1) Using the title compound (1.00 g) of Reference Example 12 and pyrrolidine (0.274 g), and in the same manner as in Example 70 (1), 3-[(2S,4S)-l-tert-butoxycarbonyl-4-(1-pyrrolidinyl)-2pyrrolidinylcarbonyl]-l,3-thiazolidine (0.793 g) was obtained as a white solid.

(2) Using the above-mentioned compound (791 mg), and in the same manner as in Example 70 (2), the title compound (626 mg) was obtained as a white powder.

124

CA 02418656 2003-02-06 ¹ H-NMR(DMSO-d₆)01.80-2.15(6H,m), 2.16-2.28(2H,m), 2.953.25(3H,m), 3.50-3.95(3H,m), 4.02-4.15(2H,m), 4.45-4.75(4H,m).

Example 126

Synthesis of 3-((2S,4S)-4-morpholino-2-pyrrolidinylcarbonyl)1,3-thiazolidine dihydrochloride (1) Using the title compound (1.00 g) of Reference Example 12 and morpholine (0.319 g), and in the same manner as in Example 70 (1), 3-((2S,4S)-l-tert-butoxycarbonyl-4-morpholino-2pyrrolidinylcarbonyl)-1,3-thiazolidine (0.987 g) was obtained as a white solid.

(2) Using the above-mentioned compound (985 mg), and in the same manner as in Example 70 (2), the title compound (746 mg) was obtained as a white powder.

¹ H-NMR(DMSO-de)02.22-2.35(2H,m), 2.90-3.50(7H,m), 3.704.20(5H,m), 4.46-4.83(6H,m), 9.30(lH,brs).

Example 127

Synthesis of 3-((2S,4S)-4-piperidino-2-pyrrolidinylcarbonyl)-

1,3-thiazolidine dihydrochloride (1) Using the title compound (1.00 g) of Reference Example 12 and piperidine (0.318 g), and in the same manner as in Example 70 (1), 3-((2S,4S)-l-tert-butoxycarbonyl-4-piperidino-2pyrrolidinylcarbonyl)-1,3-thiazolidine (0.908 g) was obtained as a white solid.

(2) Using the above-mentioned compound (906 mg), and in the same manner as in Example 70 (2), the title compound (705 mg) was obtained as a white powder.

¹ H-NMR(DMSO-dfi)01.65-1.90(6H,m), 2.18-2.34(2H,m), 2.853.20(4H,m), 3.30-3.50(2H,m), 3.55-4.05(2H,m), 4.50-4.82(6H,m).

Example 128

Synthesis of 3-((2S,4S)-4-(4-hydroxypiperidino)-2pyrrolidinylcarbonyl]-1,3-thiazolidine trihydrochloride (1) Using the title compound (988 mg) of Reference Example 12 and 4-hydroxypiperidine (867 mg), and in the same manner as in

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27103-383

Example 70 (l),3-[(2S,4S)-l-tert-butoxycarbonyl-4-(4hydroxypiperidino)-2-pyrrolidinylcarbonylj-1,3-thiazolidine (408 mg) was obtained as a white solid.

(2) Using the above-mentioned compound {408 mg), and in the same manner as in Example 70 (2), the title compound (250 mg) was obtained as a white powder.

¹H-NMR(DMSO-d_fi)61.60-1.82(2H,m),Ô1.84-2.05(2H,m), 2.122.33(2H,m), 2.85-3.55(6H,m), 3.59-4.10(6H,m), 4.45-4.78(3H,m).

Example 129 io Synthesis of 3-[(2S,4S)-4-(3-azaspiro[5.5]undec-3-yl)-2pyrrolidinylcarbonyl]-l,3-thiazolidine 2 trifluoroacetate (1) The title compound (340 mg) of Reference Example 12, 3azaspirof5.5]undecene (210 mg) and acetic acid (0.066 mL) were dissolved in 1,2-dichloroethane (10 mL), and sodium triacetoxyborohydride (485 mg) was added thereto. The mixture was stirred at room temperature for 17 hr. The reaction mixture was added to saturated aqueous sodium hydrogen * carbonate solution and the mixture was extracted with chloroform. The extract was washed with brine and dried. The »

solvent was evaporated under reduced pressure. Ethyl acetate was added to the residue, and the precipitate was collected by filtration to give 3-[(2S,4S)-4-(3-azaspiro[5.5]undec-3-yl)-ltert-butoxycarbonyl-2-pyrrolidinylcarbony1]-1,3-thiazolidine (141 mg) as a white solid.

(2) The above-mentioned compound (140 mg) was dissolved in methanol (3 mL) and chloroform (3 mL) and 4 mol/L hydrochloric acid-l,4-dioxane (1 mL) were added thereto. The mixture was stirred at room temperature for 18 hr. The solvent was evaporated under reduced pressure and the residue was purified ₃₀ by HPLC to give the title compound (52 mg) as a white solid.

¹ H-NMR{500MHz,DMSO-de)Ô1.39-1.75(14H,m), 2.08-2.14(lH,m), 2.963.30(8H,m), 3.64-3.89(3H,m), 4.05-4.10(lH,m), 4.44-4.69{3H,m), 9.84(2H,brs).

Example 130

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27103-383

Synthesis of 3-((2S,4S)-4-(4-oxo-l-phenyl-l,3,8triazaspirof4.5]dec-8-y1)-2-pyrrol.idiny lcarbony 1]-1,3thiazolidine dihydrochloride (1) Using the title compound (1.00 g) of Reference Example 12 and 4-oxo-l-phenyl-l, 3,8-triazaspiro[4.5Jdecane (0.81 g), and in the same manner as in Example 70 (1), 3-((2S,4S)-l-tertbutoxycarbonyl-4-( 4-oxo-l-phenyl-l, 3,8-triazaspiro[ 4.5]dec-8yl)-2-pyrrolidinylcarbonyl]”l,3-thiazolidine (1.64 g) was obtained as a white solid powder.

io (2) Using the above-mentioned compound (1.64 g), and in the same manner as in Example 70 (2), the title compound (0.900 g) was obtained as a white powder.

¹ H-NMR( DMSO-d₆ )01.50-2.16 ( 6H, m), 2.80-3.30( 5H,m), 3.403.95(6H,m), 4.45-4.80 ( 5H,m), 6.75-6.85(lH,m), 6.90-7 .. 05 (2H,m), 15 7.21-7.30(2H,m).

Example 131

Synthesis of 3-[(2S,4S)-4-(4-phenylpiperidino)-2pyrrolidinylcarbonyl]-1,3-thiazolidine dihydrochloride (1) Using the title compound (461 mg) of Reference Example 12 and 4-phenylpiperidine (300 mg), and in the same manner as in

Example 129 (1), 3-( (2S,4S)-l-tert-butoxycarbonyl-4-(4phenylpiperidino)-2-pyrrolidinylcarbonyl]-1,3-thiazolidine (118 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (116 mg), and in the same manner as in Example 129 (2), the title compound (78 mg) was obtained as a white powder.

¹H-NMR(DMSO-d₆ )01.98-2.06 (4H,m), 2.22-2.33 ( lH,m), 2.842.90(lH,m), 3.00-3.20(5H,m), 3.53-4.04(7H,m), 4.47-4.74(3H,m), 7.23-7.38(5H,m).

Example 132

Synthesis of 3-[(2S,4S)-4-(1,2,3,6-tetrahydro-4-phenyl-lpyridyl ) -2-pyrrolidiny lcarbony 1. ] -1,3-thiazolidine dihydrochloride

127

CA 02418656 2003-02-06 (1) Using the title compound (236 mg) of Reference Example 12 and 1,2,3,6-tetrahydro-4-phenyl-l-pyridine (150 mg), and in the same manner as in Example 70 (1), 3-[(2S,4S)-l-tertbutoxycarbonyl-4- (1,2,3,6-tetrahydro-4-phenyl-l-pyridyl)-2pyrrolidinylcarbonyl]-l,3-thiazolidine (227 mg) was obtained as a white solid.

(2) The above-mentioned compound (225 mg) was dissolved in dichloromethane (4 mL), and trifluoroacetic acid (1 mL) was added thereto at room temperature. The mixture was stirred for 16 hr. The solvent was evaporated under reduced pressure, and saturated aqueous sodium hydrogencarbonate solution was added to the residue. The mixture was extracted with chloroform. The extract was washed with brine and dried. The solvent was evaporated under reduced pressure. The residue was dissolved in ethyl acetate and 4 mol/L hydrochloric acid-ethyl acetate was added and the precipitate was collected by filtration to give the title compound (158 mg) as a white powder.

¹ H-NMR(500MHz,DMSO-d₆ )02.27-2.32(lH,m), 2.83(2H,brs), 3.033.16(4H,m), 3.68-4.15(8H,m), 4.48-4.75(3H,m), 6.19(lH,s), 7.327.41(3H,m), 7.49-7.51(2H,m).

Example 133

Synthesis of 3-{(2S,4S)-4-[4-(p-tolyl)piperidino]-2pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) The title compound (504 mg) of Reference Example 12, 4-(ptolyl)piperidine (353 mg) and acetic acid (0.096 mL) were dissolved in 1,2-dichloroethane (10 mL), and sodium triacetoxyborohydride (710 mg) was added thereto. The mixture was stirred at room temperature for 18 hr. The reaction mixture was added to water and the mixture was extracted with chloroform. The extract was washed with brine and dried. The solvent was evaporated under reduced pressure to give 3{(2S,4S)-l-tert-butoxycarbonyl-4-[4-(p-tolyl) piperidino]-2pyrrolidinylcarbonyl}-!,3-thiazolidine (115 mg) as a white

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(2) The above-mentioned compound (114 mg) was dissolved in methanol (3 mL), and chloroform (3 mL) and 4 mol/L hydrochloric acid-1,4-dioxane (1 mL) were added thereto. The mixture was stirred at room temperature for 18 hr. The solvent was evaporated under reduced pressure to give the title compound (84 mg) as a white powder.

¹H-NMR(DMSO-de)61.97-2.06(4H,m), 2.25-2.35(lH,m), 2.27(3H,s), 2.78-2.85(lH,m), 3.00-3.17(5H,m), 3.50-4.05(7H,m), 4.474.74{3H,m), 7.10-7.17(4H,m).

Example 134

Synthesis of 3-{(2S,4S)-4-(4-(3,4-xylyl)piperidino]-2pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) Using the title compound (450 mg) of Reference Example 12 and 4-(3,4-xylyl)piperidine (312 mg), and in the same manner as in Example 70 (1), 3-{(2S,4S)-1-tert-butoxycarbony1-4-[4-(3,4xylyl)piperidino]-2-pyrrolidinylcarbonyl}-1,3-thiazolidine 618 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (613 mg), and in the same manner as in Example 70 (2), the title compound (374 mg) was obtained as a white powder.

¹H-NMR(DMSO-de)61.86-2.15(4H,m), 2.18(3H,s), 2.20(3H,s), 2.222.37(lH,m), 2.68-2.74(lH,m), 2.93-3.25(5H,m), 3.42-4.07(7H,m),

4.44-4.77(3H,m), 6.88-7.04(2H,m), 7.09(lH,d,J=7.8Hz), 10.1(lH,brs), 11.91(1H,brs).

Example 135

Synthesis of 3-{(2S,4S)-4-[4-(2,4-dimethoxyphenyl)piperidino]2-pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) Using the title compound (487 mg) of Reference Example 12 and 4-(2,4-dimethoxyphenyl)piperidine (430 mg), and in the same manner as in Example 133 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(2,4-dimethoxyphenyl)piperidino]-2-pyrrolidinylcarbonyl}-

1,3-thiazolidine (82 mg) was obtained as a pale-brown solid .

129

CA 02418656 2003-02-06 (2) Using the above-mentioned compound (82 mg), and in the same manner as in Example 133 (2), the title compound (61 mg) was obtained as a brown powder.

¹ H-NMR(DMSO-d₆ )01.99-2.08(4H,m), 2.25-2.35(lH,m), 2.783.17(6H,m), 3.53-4.06(7H,m), 3.72(3H,s), 3.75(3H,s), 4.474.75(3H,m), 6.74(lH,d,J=8.1Hz), 6.83(lH,s), 6.91(lH,d,J=8.1Hz).

Example 136

Synthesis of 3-{(2S,4S)-4-[4-(2,3-dihydrobenzo[b]furan-5yl)piperidino]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) Using the title compound (450 mg) of Reference Example 12 and 4-(2,3-dihydrobenzo[b]furan-5-yl)piperidine (335 mg), and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tertbutoxycarbonyl-4- [4-(2,3-dihydrobenzo[b]furan-5-y1)piperidino]2-pyrrolidinylcarbonyl}-l,3-thiazolidine (494 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (489 mg), and in the same manner as in Example 70 (2), the title compound (330 mg) was obtained as a white powder.

¹ H-NMR(DMSO-d₆)01.84-2.14(4H,m), 2.21-2.37(lH,m), 2.722.86(lH,m), 2.96-3.26(7H,m), 3.47-4.07(7H,m), 4.44-4.77(5H,m), 6.71(lH,d,J=8.1Hz), 6.94(lH,d,J=8.1Hz), 7.09(lH,s), 9.9(lH,brs), 11.89(1H,brs).

Example 137

Synthesis of 3-{(2S,4S)-4-[4-(3,4methylenedioxyphenyl)piperidino]-2-pyrrolidinylcarbonyl}-l,3thiazolidine dihydrochloride (1) Using the title compound (505 mg) of Reference Example 12 and 4-(3,4-methylenedioxyphenyl)piperidine (414 mg), and in the same manner as in Example 133 (1), 3-{(2S,4S)-l-tertbutoxycarbonyl-4- [4-(3,4-methylenedioxyphenyl)piperidino]-2pyrrolidinylcarbonyl}-1,3-thiazolidine (105 mg) was obtained as a white solid.

130

CA 02418656 2003-02-06 (2) Using the above-mentioned compound (105 mg), and in the same manner as in Example 133 (2), the title compound (61 mg) was obtained as a white powder.

¹ H-NMR(DMSO-d₆)61.97-2.04(4H,m), 2.25-2.35(lH,m), 2.782.82(lH,m), 3.00-3.17(5H,m), 3.50-4.03(7H,m), 4.47-4.76(3H,m), 5.98(2H,s), 6.70(lH,d,J=8.1Hz), 6.82(lH,s), 6.87(lH,d,J=8.1Hz).

Example 138

Synthesis of 3-{(2S,4S)-4-[4-(4-fluoro-3methylphenyl)piperidino]-2-pyrrolidinylcarbonyl}-l,3thiazolidine dihydrochloride (1) Using the title compound (450 mg) of Reference Example 12 and 4-(4-fluoro-3-methylphenyl)piperidine (326 mg), and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tertbutoxycarbonyl-4-[4-(4-fluoro-3-methylphenyl)piperidino]-2pyrrolidinyIcarbony1&gt;-1,3-thiazolidine (603 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (597 mg), and in the same manner as in Example 70 (2), the title compound (441 mg) was obtained as a white powder.

¹ H-NMR(DMSO-d₆)01.90-2.16(4H,m), 2.23(3H,S), 2.25-2.38(lH,m),

2.77-2.92(lH,m), 2.96-3.26(5H,m), 3.48-4.07(7H,m), 4.464.78(3H,m), 7.04-7.19(3H,m), 10.5(1H,brs), 11.94(1H,brs).

Example 139

Synthesis of 3-{(2S,4S)-4-[4-(3,4-dichlorophenyl)piperidino]-2pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) Using the title compound (480 mg) of Reference Example 12 and 4-(3,4-dichlorophenyl)piperidine (442 mg), and in the same manner as in Example 133 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(3,4-dichlorophenyl)piperidino]-2-pyrrolidinylcarbonyl}-

1,3-thiazolidine (820 mg) was obtained as a white powder.

(2) Using the above-mentioned compound (820 mg), and in the same manner as in Example 133 (2), the title compound (654 mg) was obtained as a white powder.

131

CA 02418656 2003-02-06 ¹ H-NMR(DMSO-de)01.99-2.10(4H,m), 2.25-2.35(lH,m), 2.893.20(6H,m), 3.53-4.06(7H,m), 4.47-4.77(3H,m),

7.26(lH,d,J=8.1Hz), 7.50(lH,d,J=3.3Hz), 7.61(lH,dd,J=8.1,3.3Hz).

Example 140

Synthesis of 3-{(2S,4S)-4-[4-(4-chloro-3trifluoromethyIphenyl)piperidino]-2-pyrrolidinylcarbonyl}-1,3thiazolidine dihydrochloride (1) Using the title compound (503 mg) of Reference Example 12 and 4-(4-chloro-3-trifluoromethyIphenyl)piperidine (530 mg), and in the same manner as in Example 133 (1), 3-{(2s,4S)-ltert-butoxycarbony1-4-[4-(4-chloro-3- trifluoromethyIphenyl)piperidino]-2-pyrrolidinylcarbonyl}-1,3thiazolidine (189 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (189 mg), and in the same manner as in Example 133 (2), the title compound (116 mg) was obtained as a white powder.

¹H-NMR(DMSO-d₆)62.07-2.12(4H,m), 2.25-2.35(lH,m), 3.003.17(6H,m), 3.59-4.10(7H,m), 4.47-4.76(3H,m), 7.57-7.74(3H,m).

Example 141

Synthesis of 3-{(2S,4S)-4-[4-(l-naphthyl)piperidino]-2pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) Using the title compound (601 mg) of Reference Example 12 and 4-(1-naphthyl)piperidine (630 mg), and in the same manner as in Example 133 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl-4-[4(1-naphthyl)piperidino]-2-pyrrolidinylcarbonyl}-l,3thiazolidine (130 mg) as a pale-brown solid .

(2) Using the above-mentioned compound (129 mg), and in the same manner as in Example 133 (2), the title compound (72 mg) was obtained as a brown powder.

¹ H-NMR(500MHz,DMSO-d₆ )01.99-2.35(5H,m), 3.05-3.41(5H,m), 3.634.06(8H,m), 4.49-4.75(3H,m), 7.38-7.39(lH,m), 7.45-7.61(3H,m),

7.83-7.84(lH,m), 7.95-7.97(lH,m), 8.21-8.23(lH,m).

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Example 142

Synthesis of 3-{(2S,4S)-4-[4-(2-naphthyl)piperidino]-2pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) Using the title compound (450 mg) of Reference Example 12 and 4-(2-naphthyl)piperidine (349 mg), and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl-4-(4-(2naphthyl)piperidino]-2-pyrrolidinylcarbonyl}-1,3-thiazolidine (721 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (616 mg), and in the same manner as in Example 70 (2), the title compound (206 mg) was obtained as a white powder.

¹ H-NMR(DMSO-de)62.03-2.40(5H,m), 2.97-3.35(6H,m), 3.544.15(7H,m), 4.47-4.80(3H,m), 7.40-7.57(3H,m), 7.73(lH,s), 7.867.97(3H,m), 10.1(lH,brs), 11.95(lH,brs).

Example 143

Synthesis of 3-{(2S,4S)-4-[4-(2-benzo(b]thienyl)piperidino]-2pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) Using the title compound (450 mg) of Reference Example 12 and 4-(2-benzo(b]thienyl)piperidine (330 mg), and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(2-benzo[b]thienyl)piperidino]-2-pyrrolidinylcarbony1}-

1,3-thiazolidine (531 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (447 mg), and in the same manner as in Example 70 (2), the title compound (258 mg) was obtained as a white powder.

¹ H-NMR(DMSO-dg)02.03-2.40(5H,m), 2.96-4.10(13H,m), 4.464.77(3H,m), 7.23-7.42(3H,m), 7.78(lH,d,J=7.2Hz), 7.92(lH,d,J=7.5Hz), 10.3(lH,brs), 11.95(lH,brs).

Example 144

Synthesis of 3-{(2S,4S)-4-[4-(l-indolinyl)piperidino]-2pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) l-tert-Butoxycarbonylpiperidin-4-one (2.50 g), indoline (1.50 g) and acetic acid (0.73 mL) were dissolved in 1,2133

CA 02418656 2003-02-06 dichloroethane (75 mL), and sodium triacetoxyborohydride (5.32 g) was added thereto. The mixture was stirred at room temperature for 12 hr. The reaction mixture was added to iced water and the mixture was extracted with chloroform. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give l-tert-butoxycarbonyl-4-(1indolinyl)piperidine (2.82 g).

(2) The above-mentioned compound (2.82 g) was dissolved in methanol (20 mL) and 4 mol/L hydrochloric acid-1,4-dioxane (20 mL) was added thereto. The mixture was stirred at room temperature for 12 hr. The reaction mixture was added to aqueous sodium, hydrogencarbonate solution, and the mixture was extracted with chloroform. The extract was dried and the solvent was evaporated under reduced pressure to give 4-(1indolinyl)piperidine (0.60 g).

(3) Using the above-mentioned compound (470 mg) and the title compound (700 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(l-indolinyl)piperidino]-2-pyrrolidinylcarbonyl}-l,3thiazolidine (449 mg) was obtained as a white solid.

(4) Using the above-mentioned compound (448 mg), and in the same manner as in Example 70 (2), the title compound (350 mg) was obtained as a white powder.

¹ H-NMR(DMSO-d₆ )01.85-2.30(5H,m), 2.85-3.25(9H,m), 3.504.02(8H,m), 4.52-4.81(3H,m), 6.51-6.60(2H,m), 6.98-7.05(2H,m). Example 145

Synthesis of 3-{(2S,4S)-4-(4-(l-indolyl)piperidino]-2pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) Using the title compound (604 mg) of Reference Example 12 and 4-(1-indolyl)piperidine (403 mg), and in the same manner as in Example 70 (1), 3-((2S,4S)-l-tert-butoxycarbonyl-4-[4-(1indolyl)piperidino]-2-pyrrolidinylcarbonyl}-!,3-thiazolidine

134

CA 02418656 2003-02-06 (868 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (868 mg), and in the same manner as in Example 70 (2), the title compound (642 mg) was obtained as a white powder.

¹ H-NMR(DMSO-d₆)62.05-2.60(6H,m), 2.99-3.18(4H,m), 3.554.20(6H,m), 4.30-4.90(5H,m), 6.50(lH,d,J=3.0Hz), 7.05(lH, dd,J=8.2,3.0Hz), 7.16(lH,dd,J=8.1,3.0Hz), 7.35(lH,d,J=3.0Hz), 7.57(lH,d,J=8.1Hz), 7.61(lH,d,J=8.1Hz), 9.30(lH,brs), 10.00(lH,brs).

Example 146

Synthesis of 3-{(2S,4S)-4-(4-(5-bromo-l-indolinyl)piperidino]2-pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) Using l-tert-butoxycarbonylpiperidin-4-one (2.81 g) and 5bromo-l-indoline (3.00 g), and in the same manner as in Example 144 (1), 1-tert-butoxycarbonyl-4-(5-bromo-l- indolinyl)piperidine (3.34 g) was obtained.

(2) Using the above-mentioned compound (3.34 g), and in the same manner as in Example 144 (2), 4-(5-bromo-lindolinyl)piperidine (1.79 g) was obtained.

(3) Using the above-mentioned compound (1.12 g) and the title compound (1.20 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-4-[4-(5-bromo-lindoliny1)piperidino]-1-tert-butoxycarbony1-2pyrrolidinylcarbonyl}-l,3-thiazolidine (1.27 g) was obtained as a white powder.

(4) Using the above-mentioned compound (1.27 g), and in the same manner as in Example 70 (2), the title compound (0.850 g) was obtained as a white powder.

¹ H-NMR(DMSO-de)01.76-1.98(2H,m), 2.01-2.20(2H,m), 2.212.35(2H,m), 2.85-3.30(9H,m), 3.11-3.45(2H,m), 3.55-4.10(5H,m),

4.55-4.85(3H,m), 6.49(lH,d,J=8.4Hz), 7.13(lH,d,J=1.8Hz),

7.15(lH,dd,J=8.4,1.8Hz), 9.25(lH,brs).

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Example 147

Synthesis of 3-{(2S,4S)-4-[4-(2-oxo-lbenzimidazolinyl) piperidino ]-2-pyrrolidinylcarbonyl}-l, 3thiazolidine dihydrochloride (1) Using the title compound (450 mg) of Reference Example 12 and 4-(2-oxo-l-benzimidazolinyl)piperidine (358 mg), and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tertbutoxycarbonyl-4-[4-(2-oxo-l-benzimidazolinyl)piperidino]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (752 mg) was obtained as a white solid.

(2) The above-mentioned compound (635 mg) was dissolved in 1.5 mol/L hydrochloric acid-methanol (5 mL), and the mixture was stirred at room temperature for 36 hr. The reaction mixture was concentrated under reduced pressure and ethanol (10 mL) was added to the residue. The precipitate was collected by filtration to give the title compound (352 mg) as a pale-brown powder.

¹H-NMR(DMSO-de)61.83-1.98(2H,m), 2.20-2.37(lH,m), 2.722.93(2H,m), 2.96-3.45(5H,m), 3.52-4.10(7H,m), 4.46-4.77(4H,m), 7.00(3H,brs), 7.58(lH,brs), 10.97(lH,brs), 12.23(lH,brs).

Example 148

Synthesis of 3-((2S,4S)-4-(4-anilinopiperidino)-2pyrrolidinylcarbonyl]-1,3-thiazolidine trihydrochloride (1) Using l-tert-butoxycarbonylpiperidin-4-one (2.50 g) and aniline (1.24 g), and in the same manner as in Example 144 (1), 4-anilino-l-tert-butoxycarbonylpiperidine (2.35 g) was obtained.

(2) Using the above-mentioned compound (2.34 g), and in the same manner as in Example 144 (2), 4-anilinopiperidine (0.88 g) was obtained.

(3) Using the above-mentioned compound (320 mg) and the title compound (500 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-((2S,4S)-4-(4anilinopiperidino)-l-tert-butoxycarbonyl-2136

CA 02418656 2003-02-06 pyrrolidinylcarbonyl]-l,3-thiazolidine (679 mg) was obtained as a white solid.

(4) Using the above-mentioned compound (678 mg), and in the same manner as in Example 70 (2), the title compound (469 mg) was obtained as a white powder.

¹ H-NMR(DMSO-d₆ )01.98-2.45(6H,m), 2.90-3.25(6H,m), 3.304.25(5H,m), 4.50-4.85(4H,m), 6.95-7.50(5H,m), 9.22(lH,brs). Example 149

Synthesis of 3-{(2S,4S)-4-[4-(4-nitrophenyl)aminopiperidino]-2pyrrolidinylcarbonyl}-1,3-thiazolidine trihydrochloride (1) 4-Amino-1-tert-butoxycarbonylpiperazine (3.00 g), 4fluoronitrobenzene (2.54 g) and Ν,Ν-diisopropylethylamine (8.82 g) were dissolved in N-methyl-2-pyrrolidone (30 mL), and the mixture was stirred at 80°C for 18 hr. The reaction mixture was added to water and the mixture was extracted with ethyl acetate. The extract was washed with brine and dried. The solvent was evaporated under reduced pressure. The residue was purified by silica gel column chromatography to give l-tertbutoxycarbonyl-4- ( 4-nitrophenyl) aminopiperidine (2.55 g) as a white solid.

(2) Using the above-mentioned compound (1.00 g), and in the same manner as in Example 144 (2), 4-(4- nitrophenyl)aminopiperidine (0.563 g) was obtained.

(3) Using the above-mentioned compound (562 mg) and the title compound (761 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(4-nitrophenyl)aminopiperidino]-2-pyrrolidinylcarbonyl}-

1,3-thiazolidine (780 mg) was obtained as a yellow solid.

(4) Using the above-mentioned compound (778 mg), and in the same manner as in Example 70 (2), the title compound (575 mg) was obtained as a yellow powder.

¹ H-NMR(DMSO-d6)01.85-2.01(2H,m), 2.05-2.24(2H,m), 2.252.45(2H,m), 3.00-3.21(5H,m), 3.50-4.20(7H,m), 4.48-4.85(3H,m),

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6.72(2H,d,J=9.3Hz), 8.00(2H,d,J=9.3Hz), 9.21(lH,brs).

Example 150

Synthesis of 3-{(2S,4S)-4-[4-(4trifluoromethylphenyl)aminopiperidino]-2-pyrrolidinylcarbonyl}-

1,3-thiazolidine trihydrochloride (1) Using l-tert-butoxycarbonylpiperidin-4-one (2.50 g) and 4trifluoromethylaniline (2.12 g), and in the same manner as in Example 144 (1), l-tert-butoxycarbonyl-4-(4- trifluoromethylphenyl)aminopiperidine (2.23 g) was obtained.

(2) Using the above-mentioned compound (2.23 g), and in the same manner as in Example 144 (2), 4-(4- trifluoromethylphenyl)aminopiperidine (1.36 g) was obtained.

(3) Using the above-mentioned compound (447 mg) and the title compound (500 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(4-trifluoromethylphenyl) aminopiperidino]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (775 mg) was obtained as a white solid.

(4) Using the above-mentioned compound (774 mg), and in the same manner as in Example 70 (2), the title compound (514 mg) was obtained as a white powder.

¹ H-NMR(DMSO-d₆ )01.75-1.95(2H,m), 2.05-2.30(2H,m), 2.252.55(2H,m), 2.95-3.30(6H,m), 3.40-4.15(5H,m), 4.50-4.80(4H,m), 6.72(2H,d,J=8.7Hz), 7.38(2H,d,J=8.7Hz), 9.21(lH,brs).

Example 151

Synthesis of 3-{(2S,4S)-4-[4-(4-chlorophenyl)aminopiperidino]2-pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) Using l-tert-butoxycarbonylpiperidin-4-one (3.00 g) and 4chloroaniline (1.92 g), and in the same manner as in Example 144 (1), l-tert-butoxycarbonyl-4-(4- chlorophenyl)aminopiperidine (2.77 g) was obtained.

(2) Using the above-mentioned compound (2.76 g), and in the same manner as in Example 144 (2), 4-(4138

CA 02418656 2003-02-06 chlorophenyl)aminopiperidine (1.07 g) was obtained.

(3) Using the above-mentioned compound (725 mg) and the title compound (500 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(4-chlorophenyl)aminopiperidino]-2-pyrrolidinylcarbonyl}-

1,3-thiazolidine (553 mg) was obtained as an oil.

(4) Using the above-mentioned compound (550 mg), and in the same manner as in Example 70 (2), the title compound (416 mg) was obtained as a white powder.

¹ H-NMR(DMSO-ds)61.65-1.99(2H,m), 2.05-2.35(3H,m), 2.953.25(5H,m), 3.26-4.15(8H,m), 4.48-4.82(3H,m), 6.58-6.85(2H,m), 7.08-7.20(2H,m), 9.22(lH,brs).

Example 152

Synthesis of 3-{(2S,4S)-4-[4-(5-cyano-2pyridyl)aminopiperidino]-2-pyrrolidinylcarbonyl}-l,3thiazolidine trihydrochloride (1) Using the title compound (254 mg) of Reference Example 12 and 4-(5-cyano-2-pyridyl)aminopiperidine (155 mg), and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tertbutoxycarbonyl-4- [4-(5-cyano-2-pyridy1) aminopiperidino]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (225 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (224 mg), and in the same manner as in Example 70 (2), the title compound (219 mg) was obtained as a white powder.

¹ H-NMR(DMSO-de)61.80-1.99(2H,m), 2.02-2.20(2H,m), 2.212.45(2H,m), 2.98-3.23(5H,m), 3.75-4.20(7H,m), 4.55-4.86(3H,m),

6.55-6.62(lH,m), 7.72(lH,d,J=9.0Hz), 8.42(lH,s), 9.22(lH,brs).

Example 153

Synthesis of 3-{(2S,4S)-4-(4-(N-methylanilino)piperidino]-2pyrrolidinylcarbonyl}-!,3-thiazolidine trihydrochloride (1) 3-((2S,4S)-4-(4-Anilinopiperidino)-l-tert-butoxycarbonyl-2pyrrolidinylcarbonyl]-l,3-thiazolidine [product of Example 148

139

CA 02418656 2003-05-01

27103-383 *

(3), 1.18 g] was dissolved in 1,2-dichioroethane (75 mL), and sodium triacetoxyborohydride (5.32 g), acetic acid (0.73 mL) and 37% formaldehyde solution (5.0 mL) were added thereto. The mixture was stirred at room temperature for 12 hr. The reaction mixture was added to water, and the mixture was extracted with chloroform. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel column chromatography to give 3{(2S,4S)-l-tert-butoxycarbonyl-4- [ 4-(Nlo methylanilino)piperidine] -2-pyrrolidinylcarbonyl}-1,3thiazolidine (967 mg).

(2) Using the above-mentioned compound (965 mg), and in the same manner as in Example 70 (2), the title compound (618 mg) was obtained as a white powder.

¹ H-NMR(DMSO-de)ôl.75-2.35(6H,m), 2.78-3.28(9H,m), 3.404.15(6H,m), 4.48-4.85(3H,m), 7.20-7.75(5H,m), 9.22(lH,brs)„ 9.22(lH,brs).

Example 154

Synthesis of 3-{(2S,4S)-4-(4-(4-chlorophenyl)-420 hydroxypiperidino]-2-pyrrolidinylcarbonyl}-l, 3-thiazolidine 2 trifluoroacetate (1) The title compound (593 mg) of Reference Example 12 was dissolved in methanol (10 mL), and 4-(4-chlorophenyl)-4hydroxypiperidine (500 mg), acetic acid (113 pL) and sodium cyanoborohydride (124 mg) were added thereto at room temperature. The mixture was stirred for 21 hr. Water was added to the reaction mixture, and the mixture was extracted with chloroform. The extract was washed with brine and dried. The solvent was evaporated under reduced pressure to give 330 {( 2S, 4S ) - l-tert-butoxycarbonyl-4-[ 4-( 4-chlorophenyl)-4hydroxypiperidino ] -2-pyrrolidinylcarbonyl} -1,3-thiazolidine (428 mg) as a white solid.

(2) Formic acid (10 mL) was added to the above-mentioned

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CA 02418656 2003-02-06 compound (427 mg). The mixture was stirred at room temperature for 21 hr. The solvent was evaporated under reduced pressure and the residue was purified by HPLC to give the title compound (78 mg) as a white powder.

^xH-NMR(DMSO-d₆)01.81-1.85(2H,m), 2.05-2.19(3H,m), 3.004.06(12H,m), 4.45-4.71(3H,m), 5.60(lH,brs), 7.42-7.50(4H,m).

Example 155

Synthesis of 3-{(2S,4S)-4-[4-ethoxycarbonyl-4-(4fluorophenyl)piperidino]-2-pyrrolidinylcarbonyl}-1,3thiazolidine dihydrochloride (1) Using the title compound (450 mg) of Reference Example 12 and 4-ethoxycarbonyl-4-(4-fluorophenyl)piperidine (414 mg), and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tertbutoxycarbonyl-4-[4-ethoxycarbonyl-4-(4- fluorophenyl)piperidino]-2-pyrrolidinylcarbonyl}-1,3thiazolidine (742 mg) was obtained as a white solid.

(2) The above-mentioned compound (321 mg) was dissolved in ethanol (4 mL), and 4.6 mol/L hydrochloric acid-ethanol (1 mL) was added thereto. The mixture was stirred at room temperature for 18 hr. The precipitate was collected by filtration to give the title compound (218 mg) as a white powder.

¹ H-NMR(DMSO-d₆)01.14(3H,t,J=6.8Hz), 2.05-2.28(2H,m), 2.582.67(2H,m), 2.83-3.16(5H,m), 3.5-4.15(10H,m), 4.42-4.73(3H,m), 7.24(2H,t,J=8.8Hz), 7.34(2H,brs), 9.1(lH,brs), 10.35(1H,brs), 11.95(1H,brs).

Example 156

Synthesis of 3-{(2S,4S)-4-[l-(4-nitrophenyl)-4piperidinyl]amino-2-pyrrolidinylcarbonyl}-1,3-thiazolidine trihydrochloride (1) l,4-Dioxa-8-azaspiro[4,5]decane (7.88 g) was dissolved in N-methy1-2-pyrrolidine (50 mL), and diisopropylethylamine (9.58 mL) and 4-fluoronitrobenzene (7.06 g) were successively added. The mixture was stirred at room temperature for 2 hr. The

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CA 02418656 2003-02-06 reaction mixture was added to iced water, and the precipitated solid was collected by filtration to give N-(4-nitrophenyl)-4piperidone ethylene ketal (10.6 g) as a yellow powder.

(2) The above-mentioned compound (9.25 g) was suspended in acetone (100 mL), and p-toluenesulfonic acid monohydrate (7.32 g) and hydrochloric acid (20 mL) were successively added. The mixture was stirred at room temperature for 18 hr. Aqueous sodium hydroxide solution was added to the reaction mixture and the precipitate was collected by filtration to give [1-(4nitrophenyl)-4-piperidinyl]amine (6.48 g) as a yellow powder.

(3) Using the above-mentioned compound (6.17 g) and the title compound (4.22 g) of Reference Example 10, and in the same manner as in Example 70 (1), 3-((2S,4S)-1-tert-butoxycarbony14-[1-(4-nitrophenyl)-4-piperidinyl]amino-2pyrrolidinylcarbonyl}-l,3-thiazolidine (7.26 g) was obtained.

(4) The above-mentioned compound (524 mg) was dissolved in ethyl acetate (2.07 mL), and 4 mol/L hydrochloric acid-ethyl acetate (1.04 mL) was added thereto. The mixture was stirred at room temperature for 18 hr. The precipitate was collected by filtration to give the title compound (406 mg) as a yellow powder.

¹ H-NMR(DMSO-d6)61.53-1.79(2H,m), 2.02-2.25(3H,m), 2.883.01(5H,m), 3.35-3.96(5H,m), 3.96-4.28(3H,m), 4.39-4.78(3H,m), 7.08(2H,d,J=9.6Hz), 8.07(2H,d,J=9.3Hz).

Example 157

Synthesis of 3-{(2S,4S)-4-(N-methyl-N-[l-(4-nitrophenyl)-4piperidinyl]amino}-2-pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) Using 3-((2S,4S)-1-tert-butoxycarbony1-4-[1-(4nitrophenyl)-4-piperidinyl]amino-2-pyrrolidinylcarbonyl}-l,3thiazolidine [product of Example 156 (3), 1.01 g], and in the same manner as in Example 64 (1), 3-((2S,4S)-l-tertbutoxycarbonyl-4-(N-methyl-N-[1-(4-nitrophenyl)-4142

CA 02418656 2003-05-01

27103-383 piperidinyl] amino}-2-pyrrolidinylcarbonyl}-l,3-thiazolidine (1.04 g) was obtained.

(2) The above-mentioned compound (1.04 g) was dissolved in methanol (4 mL), and 4 mol/L hydrochloric acid-1,4-dioxane (2 mL) was added thereto. The mixture was stirred at room temperature for 18 hr. The reaction mixture was concentrated under reduced pressure and the obtained solid was washed with ethanol to give the title compound (0.555 g) as a yellow powder.

¹ H-NMR(DMSO-d₆ )01.50-1.90(2H,m), 1.95-2.40(3H,m), 2.68(3H,s),

2.80-3.25(5H,m), 3.25-3.98(5H,m), 4.02-4.37(3H,m), 4.404.75(3H,m), 7.09(2H,d,J=9.6Hz), 8.07(2H,d,J=9.3Hz).

Example 158

Synthesis of 3-{(2S,4S)-4-(N~(4-cyanophenylmethyl)-N-[l-(4nitrophenyl)-4-piperidinyl]amino}-2-pyrrolidinylcarbonyl}-l,315 thiazolidine dihydrochloride (1) The product (1.01 g) of Example 156 (3) was dissolved in Nmethyl-2-pyrrolidone (6 mL), and 4-cyanobenzyl bromide (0.392

g) and diisopropylethylamine (1.05 mb) were added thereto. The mixture was stirred at 80°C for 8 hr with heating. The reaction mixture was added to saturated aqueous sodium hydrogencarbonate solution and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give 3-((2S,4S)-l-tert25 butoxycarbonyl-4-(N-(4-cyanophenylmethyl)-N-[l-(4-nitrophenyl)4-piperidinyl]amino}-2-pyrrolidinylcarbonyl}-l,3-thiazolidine (0.685 g) as a yellow oil.

(2) The above-mentioned compound (1.04 g) was dissolved in ethyl acetate (4.41 mL), and 4 mol/L hydrochloric acid-ethyl acetate (2.20 mL) was added thereto. The mixture was stirred at room temperature for 18 hr. The reaction mixture was added to saturated aqueous sodium hydrogencarbonate solution and the mixture was extracted with chloroform. The extract was dried

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CA 02418656 2003-02-06 and concentrated under reduced pressure. The residue was purified by silica gel chromatography and was dissolved in chloroform. 4 mol/L Hydrochloric acid-ethyl acetate (0.309 mL) was added and the precipitate was collected by filtration to give the title compound (0.249 g) as a yellow powder.

¹ H-NMR(DMSO-de)01.34-2.35(5H,m), 2.45-3.20(7H,m), 3.204.25(8H,m), 4.35-4.80(3H,m), 7.01(2H,d,J=9.6Hz), 7.507.90(4H,m), 8.03(2H,d,J=9.3Hz), 8.87(lH,brs), 10.24(lH,brs). Example 159

Synthesis of 3-((2S,4S)-4-(4-methyl-l-piperazinyl)-2pyrrolidinylcarbonyl] -1,3-thiazolidine trihydrochloride

The title compound (450 mg) of Reference Example 12, 1methylpiperazine (0.20 mL) and acetic acid (0.09 mL) were dissolved in 1,2-dichloroethane (8 mL), and sodium triacetoxyborohydride (636 mg) was added thereto. The mixture was stirred at room temperature for 30 hr. Saturated aqueous sodium hydrogencarbonate solution was added to the reaction mixture and the mixture was extracted with ethyl acetate. The extract was washed with brine and dried. The solvent was evaporated under reduced pressure and the residue was purified by silica gel chromatography to give 3-[(2S,4S)-l-tertbutoxycarbonyl-4-(4-methyl-1-piperazinyl)-2pyrrolidinylcarbonyl]-l,3-thiazolidine (526 mg) as a white solid.

(2) The above-mentioned compound (522 mg) was dissolved in methanol (25 mL), and 1.5 mol/L hydrochloric acid-methanol (25 mL) was added thereto. The mixture was stirred at room temperature for 38 hr. The reaction mixture was concentrated under reduced pressure and ethyl acetate was added to the residue. The precipitate was collected by filtration to give the title compound (355 mg) as a white powder.

¹ H-NMR(DMSO-d₆ )01.88-2.03(lH,m), 2.79(3H,s), 2.80-2.94(lH,m),

2.98-3.93(15H,m), 4.43-4.77(3H,m), 9.10(lH,brs), 10.78(lH,brs),

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11.5(lH,brs).

Example 160

Synthesis of 3-[(2S,4S)-4-(4-phenyl-1-piperazinyl)-2pyrrolidinylcarbonyl]-1,3-thiazolidine trihydrochloride (1) Using the title compound (450 mg) and 1-phenylpiperazine (0.27 mL), and in the same manner as in Example 70 (1), 3- [(2S,4S)-l-tert-butoxycarbonyl-4-(4-phenyl-l-piperazinyl)-2pyrrolidinylcarbonyl]—1,3-thiazolidine (566 mg) was obtained as a white solid.

(2) The above-mentioned compound (442 mg) was dissolved in 1.5 mol/L hydrochloric acid-methanol (10 mL), and the mixture was stirred at room temperature for 20 hr. the reaction mixture was concentrated under reduced pressure and ethyl acetate was added to the residue. The precipitate was collected by filtration to give the title compound (418 mg) as a white powder.

¹ H-NMR(DMSO-d₆)Ô2.35(1H,q,J=11.2Hz), 2.94-3.95(15H,m), 4.034.18(lH,m), 4.44-4.77(3H,m), 6.89(1H,t,J=7.3Hz), 7.03(2H,d,J=8.0Hz), 7.28(2H,dd,J=8.0,7.3Hz), 9.23(lH,brs), 10.94(lH,brs).

Example 161

Synthesis of 1-((2S,4S)-4-(4-phenyl-l-piperazinyl)-2pyrrolidinylcarbonyl]pyrrolidine trihydrochloride (1) Using the title compound (565 mg) of Reference Example 14 and 1-phenylpiperazine (0.37 mL), and in the same manner as in Example 70 (1), 1-((2S,4S)-l-tert-butoxycarbonyl-4-(4-phenyl-lpiperazinyl)-2-pyrrolidinylcarbonyl]pyrrolidine (832 mg) was obtained as a white solid.

(2) The above-mentioned compound (700 mg) was dissolved in methanol (50 mL) and chloroform (50 mL), and 1.5 mol/L hydrochloric acid-methanol (50 mL) was added thereto. The mixture was stirred at room temperature for 5 days. The reaction mixture was concentrated under reduced pressure and

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CA 02418656 2003-02-06 methanol was added to the residue. The precipitated solid was collected by filtration to give the title compound (632 mg) as a white powder.

¹ H-NMR(DMSO-d₆ )01.73-1.98(4H,m), 2.29(lH,q,J=ll.6Hz), 2.934.18(16H,m), 4.45-4.57(lH,m), 6.89(lH,t,J=8.0Hz),

7.03(2H,d,J=8.0Hz), 7.28(2H,t,J=8.0Hz), 9.13(lH,brs), 10.89(1H,brs).

Example 162

Synthesis of 3-[(2S,4S)-4-(4-benzyl-l-piperazinyl)-2pyrrolidinyIcarbony1]-1,3-thiazolidine trihydrochloride (1) The title compound (437 mg) of Reference Example 12, 1benzylpiperazine (303 mg) and acetic acid (0.085 mL) were dissolved in 1,2-dichloroethane (6 mL), and sodium triacetoxyborohydride (650 mg) was added thereto. The mixture was stirred at room temperature for 5 hr. Saturated aqueous sodium hydrogencarbonate solution was added to the reaction mixture and the mixture was extracted with ethyl acetate. The extract was washed with brine and dried. The solvent was evaporated under reduced pressure and the residue was purified by silica gel chromatography to give 3-[(2S,4S)-4-(4-benzyl-lpiperazinyl )-l-tert-butoxycarbony1-2-pyrrolidinylcarbonyl)-1,3thiazolidine (556 mg) as a white solid.

(2) The above-mentioned compound (546 mg) was dissolved in methanol (16 mL), and 4 mol/L hydrochloric acid-1,4-dioxane (8 mL) was added thereto. The mixture was stirred at room temperature for 15 hr. The precipitate was collected by filtration to give the title compound (412 mg) as a white powder.

¹ H-NMR(DMSO-de)61.79-1.89(lH,m), 2.76-2.84(lH,m), 2.903.90(15H,m), 4.35(2H,s), 4.45-4.73(3H,m), 7.45-7.47(3H,m),

7.62-7.65(2H,m), 8.99(lH,brs), 10.45(1H,brs).

Example 163

Synthesis of 3-{(2S,4S)-4-[4-(diphenylmethyl)-l-piperazinyl)-2146

CA 02418656 2003-02-06 pyrrolidinylcarbonyl]-1,3-thiazolidine trihydrochloride (1) Using the title compound (402 mg) of Reference Example 12 and 1-diphenylmethylpiperazine (405 mg), and in the same manner as in Example 162 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl-4-[4- (diphenylmethyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-l,3thiazolidine (470 mg) was obtained as a white powder.

(2) Using the above-mentioned compound (470 mg), and in the same manner as in Example 162 (2), the title compound (449 mg) was obtained as a white powder.

¹ H-NMR(DMSO-de)61.94-2.01(lH,m), 2.79-2.85(lH,m), 3.033.92(15H,m), 4.43-4.73(3H,m), 4.48(lH,brs), 7.30-7.44(6H,m), 7.88(4H,brs), 9.09(lH,brs), 10.50(1H,brs).

Example 164

Synthesis of 3-{(2S,4S)-4-[4-(4-cyanophenyl)-l-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) Using the title compound (485 mg) of Reference Example 12 and l-(4-cyanophenyl)piperazine (335 mg), and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-[4-(4-cyanophenyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-

1,3-thiazolidine (492 mg) was obtained as a white powder.

(2) Formic acid (8 mL) was added to the above-mentioned compound (492 mg). The mixture was stirred at room temperature for 2 days. The solvent was evaporated under reduced pressure, and saturated aqueous sodium hydrogencarbonate solution was added to the residue. The mixture was extracted with chloroform. The extract was washed with brine and dried. The solvent was evaporated under reduced pressure, and the residue was purified by silica gel chromatography. 4 mol/L Hydrochloric acid-ethyl acetate was added and the precipitate was collected by filtration to give the title compound (78 mg) as a white powder.

¹ H-NMR(500MHz,DMSO-d₆ )62.20-2.27(lH,m), 2.95-3.16(3H,m), 3.104.05(13H,m), 4.47-4.74(3H,m), 7.13(lH,d,J=8.8Hz),

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7.65(lH,d,J=8.8Hz), 9.13(lH,brs), 10.61(1H,brs).

Example 165

Synthesis of 3-{(2S,4S)-4-[4-(3-trifluoromethylphenyl)-lpipera ziny1]-2-pyrrolidinylcarbonyl} -1,3-thia zolidine tr ihydroc hloride (1) Using the title compound (411 mg) of Reference Example 12 and l-(3-trifluoromethylphenyl)piperazine (378 mg), and in the same manner as in Example 162 (1), 3-{(2S,4S)-l-tertbutoxycarbonyl-4- [ 4- (3-trifluoromethylphenyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (700 mg) was obtained as a white powder.

(2) Using the above-mentioned compound (700 mg), and in the same manner as in Example 162 (2), the title compound (553 mg) was obtained as a white powder.

¹ H-NMR(DMSO-de)02.25-2.36(lH,m), 3.00-4.10(16H,m), 4.474.77(3H,m), 7.17(lH,d,J=7.8Hz), 7.26-7.33(2H,m), 7.48(lH,t,J=7.8Hz), 9.25(lH,brs), 10.82(lH,brs).

Example 166

Synthesis of 3-{(2S,4S)-4-[4-(4-methoxyphenyl)-l-piperazinyl]2-pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) Using the title compound (513 mg) of Reference Example 12 and l-(4-methoxyphenyl)piperazine (394 mg), and in the same manner as in Example 162 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(4-methoxyphenyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-

1,3-thiazolidine (536 mg) was obtained as a white powder.

(2) Using the above-mentioned compound (530 mg), and in the same manner as in Example 162 (2), the title compound (567 mg) was obtained as a white powder.

¹ H-NMR ( DMSO-d₆)62.28-2.39(lH,m), 3.00-3.17(3H,m), 3.684.12(13H,m), 3.71(3H,s), 4.47-4.77(3H,m), 6.89(2H,d,J=9.0Hz), 7.05(2H,d,J=9.0Hz), 9.22(lH,brs), 11.00(lH,brs).

Example 167

Synthesis of 3-{(2S,4S)-4-(4-(4-hydroxyphenyl)-l-piperazinyl]148

CA 02418656 2003-02-06

2-pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) Using the title compound (515 mg) of Reference Example 12 and l-(4-hydroxyphenyl)piperazine (366 mg) and in the same manner as in Example 70 (1), 3-((2S,4S)-l-tert-butoxycarbonyl4-(4-(4-hydroxyphenyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-

1,3-thiazolidine (260 mg) was obtained as a pale-brown solid .

(2) The above-mentioned compound (259 mg) was dissolved in dichloromethane (10 mL), and trifluoroacetic acid (3 mL) was added thereto. The mixture was stirred at room temperature for 16 hr. The reaction mixture was added to saturated aqueous sodium hydrogencarbonate solution, and the mixture was extracted with chloroform. The extract was washed with brine and dried. The solvent was evaporated under reduced pressure, and the residue was purified by silica gel column chromatography. Thereto was added 4 mol/L hydrochloric acidethyl acetate and the precipitate was collected by filtration to give the title compound (15 mg) as a brown powder.

¹ H-NMR(DMSO-d₆)62.18-2.28(lH,m), 2.97-4.00(16H,m), 4.464.75(3H,m), 6.73(2H,d,J=8.7Hz), 6.96-6.99(2H,m), 9.18(lH,brs), 10.51(1H,brs).

Example 168

Synthesis of 3-((2S,4S)-4-(4-(2-nitrophenyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) Piperazine (12.9 g) was dissolved in DMF (100 mL), and a solution of 2-fluoronitrobenzene (7.06 g) in DMF (30 mL) was added dropwise. The mixture was stirred at room temperature for 3 hr. The reaction mixture was added to water and the mixture was extracted with ethyl acetate. The extract was washed with water and dried. The solvent was evaporated to give 1-(2-nitrophenyl)piperazine (7.7 g) as a red oil.

(2) Using the above-mentioned compound (414 mg) and the title compound (601 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-((2S,4S)-l-tert-butoxycarbonyl149

CA 02418656 2003-02-06

4-[4-(2-nitrophenyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-

1,3-thiazolidine (690 mg) was obtained as a red oil.

(3) Using the above-mentioned compound (690 mg) and in the same manner as in Example 161 (2), the title compound (433 mg) was obtained as a yellow powder.

¹ H-NMR(DMSO-de)02.19-2.40(lH,m), 2.90-4.24(16H,m), 4.444.80(3H,m), 7.27(lH,t,J=7.5Hz), 7.41(lH,d,J=7.8Hz), 7.68(lH,t,J=7.2Hz), 7.92(lH,d,J=8.1Hz).

Example 169

Synthesis of 3-{(2S,4S)-4-[4-(4-nitrophenyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) Using the title compound (465 mg) of Reference Example 12 and l-(4-nitrophenyl)piperazine (385 mg), and in the same manner as in Example 162 (1), 3-((2S,4S)-l-tert-butoxycarbonyl4-[4-(4-nitropheny1)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-

1,3-thiazolidine (688 mg) was obtained as a yellow powder: ^XHNMR(CDC1₃)Ô1.41(4.5H,S), 1.46(4.5H,s), 1.79-1.99(lH,m), 2.382.52(lH,m), 2.52-2.74(4H,m), 2.75-3.22(3H,m), 3.25-3.50(5H,m),

3.60-4.20(3H,m), 4.36-4.82(3H,m), 6.82(2H,d,J=9.4Hz), 8.12(2H,d,J=9.4Hz).

(2) Using the above-mentioned compound (560 mg), and in the same manner as in Example 162 (2), the title compound (511 mg) was obtained as a yellow powder.

¹ H-NMR(DMSO-ds)62.23-2.32(lH,m), 2.95-3.17(3H,m), 3.574.04(13H,m), 4.47-4.76(3H,m), 7.15(2H,d,J=9.3Hz), 8.12(2H,d, J=9.3Hz), 9.19( lH,brs), 10.68( lH,brs) ; [. ]D²⁴35(cl.0,H2O).

Example 170

Synthesis of 3-((2S,4S)-4-[4-(4-fluorophenyl)-l-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) Using the title compound (409 mg) of Reference Example 12 and l-(4-fluorophenyl)piperazine (300 mg), and in the same manner as in Example 162 (1), 3-((2S,4S)-l-tert-butoxycarbonyl150

4-[4-(4-fluoropheny1)-1-pipera z iny1]-2-pyrrolidinylearbony1)1,3-thiazolidine (404 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (402 mg), and in the same manner as in Example 162 (2), the title compound (371 mg) was obtained as a white powder.

¹ H-NMR(DMSO-de)02.28-2.39(lH,m), 3.00-4.10(16H,m), 4.474.76(3H,m), 7.02-7.14(4H,m), 9.20(lH,brs), 10.79(lH,brs).

Example 171

Synthesis of 3-{(2S,4S)-4-(4-(2-chlorophenyl)-l-piperazinyl]-210 pyrrolidinylcarbony1)-1,3-thiazolidine dihydrochloride (1) Using the title compound (430 mg) of Reference Example 12 and 1-(2-chlorophenyl)piperazine (338 mg), and in the same manner as in Example 162 (1), 3-((2S,4S)-l-tert-butoxycarbonyl4-(4-(2-chlorophenyl)-1-piperaziny1]-2-pyrrolidinylcarbonyl)-

1,3-thiazolidine (687 mg) was obtained as a white powder.

(2) Using the above-mentioned compound (687 mg), and in the same manner as in Example 162 (2), the title compound (531 mg) was obtained as a white powder.

¹ H-NMR(DMSO—d₆)62.28-2.38(lH,m), 2.97-4.15(16H,m), 4.4720 4.77(3H,m), 7.12(1H,td,J=4.8,1.5Hz), 7.21(lH,dd,J=4.8,1.5Hz),

7.35(1H,td,J=8.1,1.5Hz), 7.46(lH,dd,J=8.1,1.5Hz), 9.30(lH,brs), 10.15(lH,brs).

Example 172

Synthesis of 3-{(2S,4S)-4-[4-(3-chlorophenyl)-l-piperazinyl]-225 pyrrolidinylcarbonyl)-1,3-thiazolidine trihydrochloride (1) Using the title compound (476 mg) of Reference Example 12 and l-(3-chlorophenyl)piperazine (374 mg), and in the same manner as in Example 162 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(3-chlorophenyl)-1-piperaziny1]-2-pyrrolidinylcarbonyl)-

1,3-thiazolidine (495 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (494 mg), and in the same manner as in Example 162 (2), the title compound (426 mg) was obtained as a white powder.

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CA 02418656 2003-02-06 ¹ H-NMR(DMSO-de)02.22-2.32(lH,m), 2.97-4.06(16H,m), 4.474.76(3H,m), 6.88(lH,dd,J=8.1,1.8Hz), 6.98(lH,dd,J=8.1,1.8Hz),

7.07(lH,d,J=1.8Hz), 7.27(1H,t,J=8.1Hz), 9.24(2H,brs).

Example 173

Synthesis of 3-{(2S,4S)-4-[4-(4-chlorophenyl)-l-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) Using the title compound (473 mg) of Reference Example 12 and l-(4-chlorophenyl)piperazine (372 mg), and in the same manner as in Example 162 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(4-chlorophenyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-

1,3-thiazolidine (564 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (554 mg), and in the same manner as in Example 162 (2), the title compound (533 mg) was obtained as a white powder.

¹ H-NMR(DMSO-d₆)02.25-2.35(lH,m), 2.98-3.94(15H,m), 4.044.10(lH,m), 4.45-4.75(3H,m), 7.05(2H,d,J=9.0Hz), 7.30(2H,d,J=9.0Hz), 9.19(lH,brs), 10.63(lH,brs).

Example 174

Synthesis of 3-{(2S,4S)-4-(4-(4-bromophenyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) Using the title compound (332 mg) of Reference Example 12 and 1-(4-bromophenyl)piperazine (300 mg), and in the same manner as in Example 162 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(4-bromophenyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-

1,3-thiazolidine (390 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (388 mg), and in the same manner as in Example 162 (2), the title compound (341 mg) was obtained as a white powder.

¹ H-NMR(500MHz,DMSO-dg)02.18-2.27(lH,m), 2.95-4.06(16H,m), 4.474.74(3H,m), 6.98(2H,d,J=8.8Hz), 7.40(2H,d,J=8.8Hz),

9.15(lH,brs), 10.50(lH,brs).

Example 175

Synthesis of 3-{(2S,4S)-4-[4-(3,4-dicyanophenyl)-l152

CA 02418656 2003-02-06 piperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) Using the title compound (561 mg) of Reference Example 12 and 1-(3,4-dicyanophenyl)piperazine (475 mg), and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(3,4-dicyanophenyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (945 mg) was obtained as a pale-yellow powder.

(2) Using the above-mentioned compound (935 mg), and in the same manner as in Example 167 (2), the title compound (508 mg) was obtained as a pale-yellow powder.

¹ H-NMR(DMSO-de)02.18-2.28(lH,m), 2.93-3.90(16H,m), 4.464.75(3H,m), 7.41(lH,dd,J=9.0,2.7Hz), 7.72(lH,d,J=2.7Hz), 7.90(lH,d,J=9.0Hz), 9.19(lH,brs), 10.63(lH,brs).

Example 176

Synthesis of 3-{(2S,4S)-4-[4-(3,4-dichlorophenyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) Using the title compound (540 mg) of Reference Example 12 and 1-(3,4-dichlorophenyl)piperazine (500 mg), and in the same manner as in Example 162 (1), 3-3-{(2S,4S)-l-tertbutoxycarbony1-4-[4-(3,4-dichlorophenyl)-1-piperaziny1]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (540 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (540 mg), and in the same manner as in Example 162 (2), the title compound (503 mg) was obtained as a white solid.

¹H-NMR(DMSO-d₆)02.22-2.32(lH,m), 2.97-4.06(16H,m), 4.474.76(3H,m), 7.03(lH,dd,J=9.0,2.7Hz), 7.27(lH,d,J=2.7Hz), 7.46(lH,d,J=9.0Hz), 9.20(1H,brs), 10.70(1H,brs).

Example 177

Synthesis of 3-{(2S,4S)-4-[4-(3,5-dichlorophenyl)-1pipera zinyl]-2-pyrrolidinylcarbony1}-1,3-thia zolidine

153

CA 02418656 2003-02-06 tr ihydroc hloride (1) Using the title compound (481 mg) of Reference Example 12 and 1-(3,5-dichlorophenyl)piperazine (444 mg), and in the same manner as in Example 162 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(3,5-dichloropheny1)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (523 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (520 mg), and in the same manner as in Example 162 (2), the title compound (442 mg) was obtained as a white powder.

¹H-NMR(DMSO-de)62.20-2.30(lH,m), 2.97-4.06(16H,m), 4.474.75(3H,m), 6.97(lH,s), 7.07(2H,s), 9.20(lH,brs), 10.60(lH,brs).

Example 178

Synthesis of 3-{(2S,4S)-4-[4-(4-nitro-l-naphthyl)-lpipera z iny1]-2-pyrrolidinylcarbonyl}-1,3-thia zolidine dihydrochloride (1) Using the title compound (400 mg) of Reference Example 12 and l-(4-nitro-1-naphthyl)piperazine (414 mg), and in the same manner as in Example 162 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(4-nitro-1-naphthy1)-1-pipera z iny1]-2- pyrrolidinylcarbonyl}-l,3-thiazolidine (600 mg) was obtained as a yellow powder.

(2) Using the above-mentioned compound (596 mg), and in the same manner as in Example 162 (2), the title compound (449 mg) was obtained as a pale-yellow powder.

¹H-NMR(DMSO-ds)62.29-2.39(lH,m), 3.00-3.18(3H,m), 3.574.17(13H,m), 4.48-4.78(3H,m), 7.29(lH,d,J=8.7Hz),

7.73(lH,t,J=7.8Hz), 7.83(1H,t,J=7.8Hz), 8.27(lH,d,J=8.4Hz),

8.36(lH,d,J=8.4Hz), 8.52(lH,d,J=8.7Hz), 9.20(lH,brs),

10.60(lH,brs).

Example 179

Synthesis of 3-{(2S,4S)-4-(4-(2-pyridyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-!,3-thiazolidine trihydrochloride

154

CA 02418656 2003-02-06 (1) Using the title compound (426 mg) of Reference Example 12 and l-(2-pyridyl)piperazine (0.26 mL), and in the same manner as in Example 162 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl-4-[4(2-pyridyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-l, 3thiazolidine (375 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (374 mg), and in the same manner as in Example 162 (2), the title compound (466 mg) was obtained as a white powder.

¹ H-NMR(DMSO-d₆ )02.26-2.37(lH,m), 3.00-3.16(3H,m), 3.434.06(13H,m), 4.47-4.78(3H,m), 6.98(lH,t,J=6.0Hz), 7.35(lH,d,J=9.0Hz), 7.96(1H,td,J=9.0,1.5Hz),

8.13(lH,dd,J=6.0,1,5Hz), 9.23(lH,brs), 10.98(lH,brs).

Example 180

Synthesis of 3-{(2S,4S)-4-[4-(4-pyridyl)-l-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) Using the title compound (601 mg) of Reference Example 12 and l-(4-pyridyl)piperazine (326 mg), and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl-4-[4-(4pyridyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-l,3thiazolidine (366 mg) was obtained.

(2) Using the above-mentioned compound (366 mg), and in the same manner as in Example 161 (2), the title compound (133 mg) was obtained as a white powder.

¹ H-NMR(DMSO-dfi)02.03-2.30(lH,m), 2.79-4.30(16H,m), 4.404.80(3H,m), 7.32(2H,d,J=7.5Hz), 8.34(2H,d,J=7.2Hz),

9.15(lH,brs), 10.80(lH,brs), 14.00(lH,brs).

Example 181

Synthesis of 3-{(2S,4S)-4-[4-(4-cyano-2-pyridyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine tr ihydroc hloride (1) Using the title compound (494 mg) of Reference Example 12 and l-(4-cyano-2-pyridyl)piperazine (371 mg), and in the same manner as in Example 154 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl155

CA 02418656 2003-02-06

4-(4-(4-cyano-2-pyridyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (431 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (424 mg), and in the same manner as in Example 167 (2), the title compound (194 mg) was obtained as a pale-yellow powder.

¹ H-NMR(500MHz,DMSO-d₆ )02.32-2.39(lH,m), 3.00-3.16(3H,m), 3.254.07(13H,m), 4.48-4.75(3H,m), 7.10(lH,dd,J=5.1,0.8Hz), 7.50(lH,d,J=0.8Hz), 8.36(lH,d,J=5.1Hz), 9.22(lH,brs),

10.91(lH,brs).

Example 182

Synthesis of l-{(2S,4S)-4-[4-(4-cyano-2-pyridyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}pyrrolidine trihydrochloride (1) Using the title compound (527 mg) of Reference Example 14 and l-(4-cyano-2-pyridyl)piperazine (422 mg), and in the same manner as in Example 70 (1), l-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(4-cyano-2-pyridyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}pyrrolidine (502 mg) was obtained as a pale-yellow solid.

(2) Using the above-mentioned compound (491 mg), and in the same manner as in Example 167 (2), the title compound (134 mg) was obtained as a pale-yellow powder.

¹ H-NMR(500MHz,DMSO-de)01.83-1.96(4H,m), 2.25-2.30(lH,m), 2.983.02(lH,m), 3.20-3.56(10H,m), 3.70-3.72(2H,m), 4.04-4.08(lH,m),

4.30-4.54(2H,m), 4.50-4.54(lH,m), 7.10(lH,dd,J=5.1,0.8Hz), 7.50(lH,d,J=0.8Hz), 8.36(lH,d,J=5.1Hz), 9.12(lH,brs),

10.73(lH,brs).

Example 183

Synthesis of 3-{(2S,4S)-4-[4-(5-cyano-2-pyridyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine tr ihydrochloride (1) Using the title compound (740 mg) of Reference Example 12

156

CA 02418656 2003-02-06 and l-(5-cyano-2-pyridyl)piperazine (516 mg), and in the same manner as in Example 154 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(5-cyano-2-pyridyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (772 mg) was obtained as a white powder.

¹ H-NMR(CDCl₃)ôl .41 ( 4.5H,s), 1.46 (4.5H, s), 1.86-1.98 ( lH,m),

2.45-2.60(5H,m), 2.83-3.25(3H,m), 3.31-3.39(lH,m), 3.603.79(5H,m), 3.81-3.99(2H,m), 4.40-4.85(3H,m),

6.58(lH,d,J=9.0Hz), 7.61(lH,dd,J=9.0,2.1Hz), 8.40(1H,d,J=2.1Hz).

(2) Using the above-mentioned compound (744 mg), and in the same manner as in Example 167 (2), the title compound (202 mg) was obtained as a pale-yellow powder.

¹H-NMR(DMSO-de)02.28-2.39(lH,m), 2.97-3.16(3H,m), 3.354.10(13H,m), 4.47-4.76(3H,m), 7.11(lH,d,J=9.3Hz), 7.98(lH,dd,J=9.3,2.1Hz), 8.57(lH,d,J=2.1Hz), 9.25(lH,brs), 10.91( lH,brs) ;[. ]d²³- 32 (cl.0,H2O) .

Example 184

Synthesis of 3-{(2S,4S)-4-(4-(5-trifluoromethy1-2-pyridyl)-1piperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) Piperazine (12.9 g) was suspended in N-methyl-2-pyrrolidone (130 mL), and a solution of 2-chloro-5-trifluoromethylpyridine (9.08 g) in N-methy1-2-pyrrolidone (30 mL) was added dropwise. The mixture was stirred at room temperature for 18 hr. The reaction mixture was added to water and the mixture was extracted with ethyl acetate. The extract was washed with water and dried. The solvent was evaporated under reduced pressure to give l-(5-trifluoromethyl-2-pyridyl)piperazine (11.5 g) as a white solid.

(2) Using the above-mentioned compound (462 mg) and the title compound (601 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(5-trifluoromethy1-2-pyridyl)-1-piperazinyl]-2157

CA 02418656 2003-02-06 pyrrolidinylcarbonyl}-l,3-thiazolidine (379 mg) as an oil.

(3) Using the above-mentioned compound (368 mg), and in the same manner as in Example 161 (2), the title compound (276 mg) was obtained as a white powder.

¹ H-NMR(DMSO-de)02.20-2.48(lH,m), 2.87-5.00(19H,m),

7.14(lH,d,J=9.0Hz), 7.92(lH,dd,J=9.3,2.4Hz), 8.49(lH,d,J=0.6Hz).

Example 185

Synthesis of 3-{(2S,4S)-4-[4-(5-nitro-2-pyridyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-1,3-thiazolidine dihydrochloride (1) Using 2-chloro-5-nitropyridine (7.93 g), and in the same manner as in Example 184 (1), l-(5-nitro-2-pyridyl)piperazine (9.3 g) was obtained as a yellow powder.

(2) Using the above-mentioned compound (416 mg) and the title compound (601 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(5-nitro-2-pyridyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (754 mg) was obtained as a yellow powder.

(3) Using the above-mentioned compound (693 mg), and in the same manner as in Example 161 (2), the title compound (475 mg) was obtained as a yellow powder.

¹ H-NMR(DMSO-de)62.00-2.34(lH,m), 2.75-4.10(16H,m), 4.404.80(3H,m), 7.09(lH,d,J=9.6Hz), 8.32(lH,dd,J=9.6,3.0Hz), 9.01(lH,d,J=3.0Hz), 9.18(lH,brs), 10.50(lH,brs).

Example 186

Synthesis of l-{(2S,4S)-4-(4-(5-nitro-2-pyridyl)-1piperazinyl]-2-pyrrolidinylcarbonyl}pyrrolidine trihydrochloride (1) Using 1-(5-nitro-2-pyridyl)piperazine [product of Example 185 (1), 625 mg] and the title compound (565 mg) of Reference Example 14, and in the same manner as in Example 70 (1), 1{(2S,4S)-l-tert-butoxycarbonyl-4-[4-(5-nitro-2-pyridyl)-1158

CA 02418656 2003-02-06 piperazinyl]-2-pyrrolidinylcarbonyl}pyrrolidine (632 mg) was obtained as a yellow powder.

(2) The above-mentioned compound (522 mg) was dissolved in methanol (10 mL) and chloroform (2.5 mL), and 4 mol/L hydrochloric acid-ethyl acetate (5 mL) was added thereto. The mixture was stirred at room temperature for 18 hr. The reaction mixture was concentrated under reduced pressure, and the residue was washed with ethanol to give the title compound (395 mg) as a yellow powder.

¹ H-NMR(DMSO-d₆)61.70-2.04(4H,m), 2.09-2.36(lH,m), 2.863.07(lH,m), 3.20-5.00(14H,m), 7.11(lH,d,J=9.6Hz),

8.33(lH,dd,J=9.6,3.0Hz), 9.02(lH,d,J=2.7Hz), 9.11(lH,brs),

10.80(lH,brs).

Example 187

Synthesis of 3-{(2S,4S)-4-[4-(5-chloro-2-pyridyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) Using the title compound (340 mg) of Reference Example 12 and l-(5-chloro-2-pyridyl)piperazine (268 mg), and in the same manner as in Example 162 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(5-chloro-2-pyridyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (421 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (418 mg), and in the same manner as in Example 162 (2), the title compound (262 mg) was obtained as a white powder.

¹ H-NMR(500MHz,DMSO-de)62.26-2.31(lH,m), 2.97-4.40(16H,m), 4.474.73(3H,m), 7.03(lH,d,J=9.1Hz), 7.71(lH,dd,J=9.1,2.5Hz), 8.18(lH,d,J=2.5Hz), 9.18(lH,brs), 10.57(lH,brs).

Example 188

Synthesis of 3-{(2S,4S)-4-(4-(2-quinolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) Using the title compound (488 mg) of Reference Example 12

159

CA 02418656 2003-02-06 and l-(2-quinolyl)piperazine (416 mg), and in the same manner as in Example 162 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl-4-(4(2-quinolyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-l,3thiazolidine (724 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (720 mg), and in the same manner as in Example 162 (2), the title compound (560 mg) was obtained as a white powder.

¹ H-NMR(DMSO-d₆ )02.20-2.30(lH,m), 2.96-3.17(3H,m), 3.644.40(13H,m), 4.47-4.76(3H,m), 7.50(lH,t,J=7.5Hz),

7.60(lH,d,J=9.6Hz), 7.77(lH,t,J=7.8Hz), 7.93(lH,d,J=7.5Hz),

8.15-8.20(lH,m), 8.44(lH,d,J=9.6Hz), 9.21(lH,brs),

10.68(lH,brs).

Example 189

Synthesis of 3-{(2S,4S)-4-[4-(4-quinolyl)-l-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) Piperazine (13.2 g) was melted by heating at 140°C, and 4chloroquinoline (2.5 g ) was added thereto. The mixture was stirred at 140°C for 30 min. The reaction mixture was added to iced water, and the mixture was extracted with chloroform to give l-(4-quinolyl)piperazine (3.45 g) as a pale-yellow oil.

(2) Using the above-mentioned compound (469 mg) and the title compound (601 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(4-quinolyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-l,3thiazolidine (995 mg) was obtained.

(3) Using the above-mentioned compound (995 mg), and in the same manner as in Example 186 (2), the title compound (392 mg) was obtained as a white powder.

¹ H-NMR(DMSO-d6)02.16-2.40(lH,m), 2.70-4.30(16H,m), 4.404.80(3H,m), 7.37(lH,d,J=6.9Hz), 7.77(lH,t,J=8.1Hz),

8.04(1H,t,J=8.4Hz), 8.21(lH,d,J=8.7Hz), 8.85(lH,d,J=6.9Hz).

Example 190

Synthesis of 3-{(2S,4S)-4-(4-(1-isoquinolyl)-1-piperazinyl]-2160

CA 02418656 2003-02-06 pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) Using the title compound (606 mg) of Reference Example 12 and 1-(1-isoquinolyl)piperazine (692 mg), and in the same manner as in Example 162 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(1-isoquinolyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-

1,3-thiazolidine (216 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (215 mg), and in the same manner as in Example 162 (2), the title compound (99 mg) was obtained as a pale-yellow powder.

¹ H-NMR(500MHz,DMSO-de)02.25-2.30(lH,m), 3.00-3.17(3H,m), 3.593.95(12H,m), 4.13-4.18(lH,m), 4.49-4.77(3H,m),

7.59(lH,d,J=6.1Hz), 7.71-7.74(lH,m), 7.86-7.89(lH,m), 8.02(lH,d,J=8.2Hz), 8.08(lH,d,J=6.1Hz), 8.21(lH,d,J=8.5Hz), 9.25(lH,brs), 10.89(1H,brs).

Example 191

Synthesis of 3-{(2S,4S)-4-[4-(2-trifluoromethyl-4-quinolyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) Using the title compound (0.655 g) of Reference Example 12 and l-(2-trifluoromethyl-4-quinolyl)piperazine (0.735 g), and in the same manner as in Example 162 (1), 3-{(2S,4S)-l-tertbutoxycarbonyl-4- [4-(2-trifluoromethyl-4-quinolyl)-1piperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine (1.23 g) was obtained as a pale-yellow powder: ^XH-NMR(DMSO- d₆)Ô1.33(4.5H,s), 1.41(4.5H,s), 1.55-1.64(lH,m), 2.602.78(5H,m), 2.90-3.15(4H,m), 3.33-3.38(4H,m), 3.67-3.85(3H,m), 4.04-4.69(3H,m), 7.25(1H,S), 7.70(1H,t,J=8.1Hz), 7.817.87(lH,m), 8.07(2H,d,J=8.4Hz).

(2) Using the above-mentioned compound (1.23 g), and in the same manner as in Example 162 (2), the title compound (1.06 g) was obtained as a pale-yellow powder.

¹ H-NMR(DMSO-d₆ )02.28-2.38(lH,m), 3.00-3.18(3H,m), 3.484.15(13H,m), 4.48-4.78(3H,m), 7.39(lH,s), 7.75(lH,t,J=7.5Hz),

161

CA 02418656 2003-02-06

7.89(1H,t,J=7.5Hz), 8.11-8.16(2H,m), 9.23(lH,brs),

10.68(lH,brs); [. ]_D²⁴-32(cl.0,H₂O).

Example 192

Synthesis of 3-{(2S,4S)-4-[4-(2-benzoxazolyl)-l-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) Using 2-chlorobenzoxazole (7.68 g) and piperazine (12.9 g), and in the same manner as in Example 168 (1), 1-(2benzoxazolyl)piperazine (2.4 g) was obtained as a white solid.

(2) Using the above-mentioned compound (610 mg) and the title compound (601 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-4-[4-(2-benzoxazolyl)-

1- tert-butoxycarbony1-1-piperazinyl]-2-pyrrolidinylcarbonyl}-

1,3-thiazolidine (526 mg) was obtained as a white powder.

(3) Using the above-mentioned compound (416 mg), and in the same manner as in Example 186 (2), the title compound (286 mg) was obtained as a white powder.

¹H-NMR(DMSO-dfi)62.20-2.42(lH,m), 2.89-3.20(3H,m), 3.254.35(13H,m), 4.40-4.80(3H,m), 7.10(lH,td,J=7.5,1.2Hz), 7.22(lH,td,J=7.8,1.2Hz), 7.37(lH,dd,J=7.8,0.6Hz), 7.47(lH,d,J=7.8Hz), 9.25(lH,brs), 11.00(lH,brs).

Example 193

Synthesis of 3-{(2S,4S)-4-[4-(2-benzothiazolyl)-l-piperazinyl]-

2- pyrrolidinylcarbonyl}-1,3-thiazolidine trihydrochloride (1) Using 2-chlorobenzothiazole (8.48 g) and piperazine (43.1 g), and in the same manner as in Example 189 (1), 1-(2benzothiazolyl)piperazine (10.9 g) was obtained.

(2) Using the above-mentioned compound (482 mg) and the title compound (601 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-4-[4-(2benzothiazolyl)-1-piperazinyl]-l-tert-butoxycarbonyl-2pyrrolidinylcarbonyl}-l,3-thiazolidine (798 mg) was obtained as a white powder.

(3) Using the above-mentioned compound (606 mg), and in the

162

CA 02418656 2003-02-06 same manner as in Example 186 (2), the title compound (591 mg) was obtained as a white powder.

¹ H-NMR(DMSO-dg)02.20-2.44(lH,m), 2.90-3.20(3H,m), 3.354.30(13H,m), 4.42-4.82(3H,m), 7.16(lH,t,J=7.2Hz),

7.35(1H,t,J=7.2Hz), 7.55(lH,d,J=7.8Hz), 7.86(lH,d,J=7.5Hz),

9.25(lH,brs), 10.90(1H,brs).

Example 194

Synthesis of 3-{(2S,4S)-4-[4-(benz-2-oxa-l,3-diazol-5-yl)-lpipera z iny1]-2-pyrrolidinylcarbony1}-1,3-thia zolidine dihydrochloride (1) Using 5-chlorobenzofurazan (0.500 g) and piperazine (2.79 g), and in the same manner as in Example 189 (1), l-(benz-2oxa-1,3-diazol-5-yl)piperazine (0.433 g) was obtained.

(2) Using the above-mentioned compound (433 mg) and the title compound (601 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-4-(4-(benz-2-oxa-1,3diazol-5-yl)-1-tert-butoxycarbonyl-l-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (500 mg) was obtained as a yellow powder.

(3) Using the above-mentioned compound (438 mg), and in the same manner as in Example 186 (2), the title compound (409 mg) was obtained as a yellow powder.

¹ H-NMR(DMSO-de)62.10-2.40(lH,m), 2.80-4.30(16H,m), 4.404.80(3H,m), 7.02(lH,s), 7.72(lH,dd,J=9.9,1.5Hz), 7.94(lH,d,J=9.9Hz).

Example 195

Synthesis of 3-{(2S,4S)-4-(4-(4-nitrobenzoyl)-l-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) Using the title compound (454 mg) of Reference Example 12 and l-(4-nitrobenzoyl)piperazine (426 mg), and in the same manner as in Example 162 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(4-nitrobenzoyl)-1-piperazinyl]-2-pyrrolidinylcarbony1}-

1,3-thiazolidine (500 mg) was obtained as a white solid.

163

CA 02418656 2003-02-06 (2) Using the above-mentioned compound (496 mg), and in the same manner as in Example 162 (2), the title compound (242 mg) was obtained as a white powder.

¹ H-NMR(DMSO-de)02.15-2.22(lH,m), 2.90-2.94(lH,m), 3.073.93(15H,m), 4.46-4.73(3H,m), 7.75(2H,d,J=8.6Hz), 8.32(2H,d,J=8.6Hz), 9.15(lH,brs), 10.63(lH,brs).

Example 196

Synthesis of 3-{(2S,4S)-4-[4-(4-nitrophenyl)-l,4-diazepam-lyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) Homopiperazine (15.0 g) was dissolved in N-methyl-2pyrrolidine (50 mL), and 4-fluoronitrobenzene (7.06 g) was added thereto. The mixture was stirred at room temperature for 30 min. The reaction mixture was added to iced water, and the precipitate was collected by filtration to give N-(4nitropheny1)-1,4-diazepam (10.9 g) as a yellow powder.

(2) Using the above-mentioned compound (443 mg) and the title compound (601 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert.butoxycarbonyl4-[4-(4-nitrophenyl)-l,4-diazepam-l-yl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (429 mg) was obtained as a yellow powder.

(3) Using the above-mentioned compound (398 mg), and in the same manner as in Example 161 (2), the title compound (118 mg) was obtained as a yellow powder.

¹ H-NMR(DMSO-d₆ )01.80-2.60(3H,m), 2.70-4.20(12H,m), 4.384.78(3H,m), 6.89(2H,d,J=9.3Hz), 8.09(2H,d,J=9.3Hz).

Example 197

Synthesis of 3-{(2S,4S)-4-[4-(2-trifluoromethyl-4-quinolyl)-

1,4-diazepam-l-yl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) Using 4-chloro-2-trifluoromethylquinoline (5.00 g) and homopiperazine (20.7 g), and by reaction in the same manner as in Example 196 (1) at 60°C, N-(2-trifluoromethyl-4-quinolyl)164

CA 02418656 2003-02-06

1,4-diazepam (6.11 g) was obtained as a yellow solid.

(2) Using the above-mentioned compound (0.83 g) and the title compound (0.703 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-[4-(2-trifluoromethyl-4-guinolyl)-1,4-diazepam-l-yl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (1.35 g) was obtained as a white powder.

(3) Using the above-mentioned compound (1.35 g), and in the same manner as in Example 133 (2), the title compound (1.21 g) was obtained as a pale-yellow powder.

¹ H-NMR(500MHz,DMSO-d₆ )02.19-2.49(3H,m), 2.96-3.19(3H,m), 3.304.28(13H,m), 4.48-4.76(3H,m), 7.17(lH,s), 7.67(1H,t,J=7.6Hz), 7.83(lH,t,J=7.6Hz), 8.05-8.10(2H,m), 9.14(1H,brs),

10.91(1H,brs), 12.51(lH,brs)

Example 198

Synthesis of 3-{(2S,4S)-4-[4-(2-hydroxyethyl)-l-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) Using 1-piperazineethanol (147 mg) and the title compound (307 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl-4-[4-(2hydroxyethyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-1,3thiazolidine (354 mg) was obtained as an oil.

(2) Using the above-mentioned compound (350 mg), and in the same manner as in Example 132 (2), deprotection was conducted. The solvent was evaporated under reduced pressure, and the residue was dissolved in ethyl acetate (5 mL). 4 mol/L

Hydrochloric acid-ethyl acetate (1.0 mL) was added thereto, and the precipitated solid was collected by filtration to give the title compound (158 mg) as a white powder.

¹ H-NMR(500MHz,DMSO-de)01.91( lH,m), 2.78-3.93(20H,m), 4.464.74{3H,m), 8.97(lH,brs), 10.80(lH,brs), 11.97(1H,brs).

Example 199

Synthesis of 3-[(2S,4S)-4-(4-pivaloyl-l-piperazinyl)-2165

CA 02418656 2003-02-06 pyrrolidinylcarbonyl]-1,3-thiazolidine dihydrochloride (1) Using N-benzyloxycarbonyl-L-trans-4-hydroxyproline (25.0 g), and in the same manner as in Reference Examples 9 and 12, 3- ({2S)-1-benzyloxycarbonyl-4-oxo-2-pyrrolidinylcarbony1)-1,3thiazolidine (10.9 g) was obtained as white crystals.

(2) Using the above-mentioned compound (4.05 g) and 1-tertbutoxy carbonylpiper a zine (2.48 g), and in the same manner as in Example 70 (1), 3-[(2S,4S)-1-benzyloxycarbony1-4-(4-tertbutoxycarbonyl-l-piperazinyl)-2-pyrrolidinylcarbonyl]-1,3thiazolidine (4.64 g) was obtained as a white powder.

(3) The above-mentioned compound (4.04 g) was deprotected in the same manner as in Example 132 (2). The solvent was evaporated under reduced pressure, and saturated aqueous sodium hydrogencarbonate solution was added to the residue. The mixture was extracted with chloroform, and the extract was dried and concentrated under reduced pressure to give 3- [(2S,4S)-1-benzyloxycarbony1-4-(1-piperazinyl)-2pyrrolidinylcarbonyl]-l,3-thiazolidine (3.10 g) as a white powder.

(4) The above-mentioned compound (405 mg) and triethylamine (170 μΙ&gt;) were dissolved in chloroform (4 mL), and pivaloyl chloride (126 μL) was added thereto at room temperature. The mixture was stirred for 3 hr. Water was added to the reaction mixture and the mixture was extracted with chloroform. The extract was dried and the mixture was concentrated under reduced pressure to give 3-[(2S,4S)-l-benzyloxycarbonyl-4-(4pivaloyl-1-piperazinyl)-2-pyrrolidinylcarbonyl]-1,3thiazolidine (430 mg) as a white solid.

(5) The above-mentioned compound (423 mg) was dissolved in trifluoroacetic acid, and thioanisole (0.6 mL) was added thereto. The mixture was stirred at room temperature for 21 hr. Diethyl ether was added to the reaction mixture, and the precipitated solid was collected by filtration and purified by

166

CA 02418656 2003-02-06

HPLC. This was converted to hydrochloride with 4 mol/L hydrochloric acid-ethyl acetate to give the title compound (95 mg) as a white powder.

¹ H-NMR(500MHz,DMSO-d₆)01.20(9H,S), 2.12-2.33(lH,m), 2.854.05(16H,m), 4.48-4.73(3H,m), 9.08(lH,brs), 10.73(lH,brs).

Example 200

Synthesis of 3-((2S_Z4S)-4-(4-methoxycarbonyl-1-piperazinyl)-2pyrrolidinylcarbonyl]-1,3-thiazolidine dihydrochloride (1) Using 3-[(2S,4S)-1-benzyloxycarbony1-4-(1-piperazinyl)-2pyrrolidinylcarbonyl]-l,3-thiazolidine [product of Example 199 (3), 405 mg] and methyl chlorocarbonate (79 pL), and in the same manner as in Example 199 (4), 3-[(2S,4S)-1benzyloxycarbonyl-4-(4-methoxycarbonyl-l-piperazinyl)-2pyrrolidinylcarbonyl]-l,3-thiazolidine (413 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (407 mg), and in the same manner as in Example 199 (5), the title compound (43 mg) was obtained as a brown powder.

¹ H-NMR(500MHz,DMSO-d₆)δ2.13-2.43(lH,m), 2.85-4.05(16H,m), 3.63(3H,s), 4.47-4.74(3H,m), 9.08(lH,brs), 10.73(lH,brs).

Example 201

Synthesis of 3-[(2S,4S)-4-(4-isobutyloxycarbony1-1piperaziny1)-2-pyrrolidinylcarbonyl]-1,3-thiazolidine dihydrochloride (1) Using the product (405 mg) of Example 199 (3) and isobutyl chlorocarbonate (133 pL), and in the same manner as in Example 199 (4), 3-[(2S,4S)-l-benzyloxycarbonyl-4-(4isobutyloxycarbonyl-1-piperazinyl)-2-pyrrolidinylcarbonyl]-1,3thiazolidine (421 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (416 mg), and in the same manner as in Example 199 (5), the title compound (59 mg) was obtained as a white powder.

¹ H-NMR(500MHz,DMSO-de)60.90(6H,d,J=6.8Hz), 1.81-1.97(lH,m),

167

CA 02418656 2003-05-01

27103-383

2.19-2.39(lH,m), 2.90-4.20(16H,m), 3.83(2H,d,J=6.5Hz), 4.474.74(3H,m), 9.12(lH,brs), 11.07(lH,brs).

Example 202

Synthesis of 3-((2S,45)-4-(4-benzyloxycarbonyl-l-piperazinyl)5 2-pyrrolidinylcarbonyl]-1,3-thiazolidine dihydrochloride (1) Using 1-benzyloxycarbonylpiperazine (217 mg) and the title compound (307 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-[ (2S,4S)-4-(4-benzyloxycarbonyl1-piperazinyl)-1-tert-butoxycarbony1-2-pyrrolidinylcarbonyl]- io 1,3-thiazolidine (500 mg) was obtained as a white powder.

(2) Using the above-mentioned compound (490 mg), and in the same manner as in Example 133 (2), the title compound (399 mg) was obtained as a white powder.

¹ H-NMR(500MHz,DMSO-de)62.10-2.30(lH,m), 1 .70-4.20(16H,m), 4.4615 4.73(3H,m), 5.11(2H,s), 7.31-7.42(5H,m), 9.06(lH,brs),

10.67(lH,brs), 12.50(lH,brs).

Example 203

Synthesis of 3-[(2S,4S)-4-(4-cyclohexylaminocarbonyl-lpiperazinyl) -2-pyrrolidiny lcarbonyl] -1,3-thiazolidine dihydrochloride (1) Using the product (405 mg) of Example 199 (3) and cyclohexyl isocyanate (127 |iL), and in the same manner as in Example 199 (4), 3-[(2S,4S)-l-benzyloxycarbonyl-4-(4cyclohexylaminocarbonyl-1-piperazinyl) -2-pyrrolidiny lcarbonyl] -

1,3-thiazolidine (296 mg) was obtained as a white solid.

(2) Using the above-mentioned compound (296 mg), and in the same manner as in Example 199 (5), the title compound (85 mg) was obtained as a white powder.

¹ H-NMR(500MHz,DMSO-de)61.00-1.29(5H,m), 1.53-1.80(5H,m), 2.1230 2.32(lH,m), 2.80-4.20(17H,m), 4.47-4.73(3H,m), 6.48(lH,brs), 9.09(lH,brs), 10.65(lH,brs), 12.18(lH,brs).

Example 204

Synthesis of 3-{(2S,4S)-4-[4-(2,6-dimethylphenyl)aminocarbonyl168

CA 02418656 2003-02-06

1-piperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) Using the product (405 mg) of Example 199 (3) and 2,6dimethylphenyl isocyanate (142 μΣ), and in the same manner as in Example 199 (4), 3-{(2S,4S)-l-benzyloxycarbonyl-4-[4-(2,6dimethylphenyl)aminocarbonyl-l-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (517 mg) was obtained as a white powder.

(2) Using the above-mentioned compound (503 mg), and in the io same manner as in Example 199 (5), the title compound (166 mg) was obtained as a white powder.

¹ H-NMR(500MHz,DMSO-d₆)62.15(6H,S), 2.09-2.29(lH,m), 2.854.20(16H,m), 4.48-4.73(3H,m), 7.04(3H,m), 8.16(lH,s), 9.07(lH,brs), 10.61(lH,brs).

Example 205

Synthesis of 3-{(2S,4S)-4-(4-(8-quinolinesulfonyl)-1piperazinyl]-2-pyrrolidinylcarbonyl}-1,3-thiazolidine tr ihydroc hloride (1) 1-tert-Butoxycarbonylpiperazine (2.22 g) and triethylamine (2.0 mL) were dissolved in dichloromethane (100 mL), and 8quinolinesulfonyl chloride (2.71 g) was added thereto. The mixture was stirred at room temperature for 14 hr. 10% citric acid solution was added to the reaction mixture and the mixture was extracted with chloroform. The extract was washed with brine, dried and concentrated under reduced pressure. The residue was dissolved in dichloromethane (20 mL), and trifluoroacetic acid (5 mL) was added thereto. The mixture was stirred at room temperature for 1.5 hr. The reaction mixture was concentrated under reduced pressure, and saturated aqueous sodium hydrogencarbonate solution was added to the residue.

The mixture was extracted with chloroform, and the extract was dried and concentrated under reduced pressure to give 1-(8quinolinesulfonyl)piperazine (0.73 g) as a white solid.

169

CA 02418656 2003-02-06 (2) Using the above-mentioned compound (0.725 g) and the title compound (0.714 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(8-quinolinesulfonyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (1.34 g) was obtained as a white powder.

(3) Using the above-mentioned compound (1.34 g), and in the same manner as in Example 133 (2), the title compound (0.56 g) was obtained as a pale-yellow powder.

¹ H-NMR(500MHz,DMSO-de)62.01-2.21(lH,m), 2.80-3.95(16H,m), 4.434.72(3H,m), 7.74(1H,dd,J=8.3,4.2Hz), 7.79(1H,t,J=7.9Hz), 8.358.40(2H,m), 8.58(lH,dd,J=8.3,1.7Hz), 9.00(lH,brs), 9.06(lH,dd,J=4.2,1.7Hz), 10.60(lH,brs).

Example 206

Synthesis of 3-{(2S,4S)-4-[4-(l-ethoxycarbonyl-4-piperidinyl)l-piperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine tetrahydrochloride (1) Using 1-(9-fluorenylmethoxycarbonyl)piperazine (1.47 g) and the title compound (1.30 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tertbutoxycarbonyl-4- [4-(9-fluorenylmethoxycarbonyl)-1piperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine (1.68 g) was obtained as a white powder.

(2) The above-mentioned compound (1.68 g) was dissolved in dichloromethane (30 mL), and piperidine (1.5 mL) was added thereto at room temperature. The mixture was stirred for 4 hr. The solvent was evaporated under reduced pressure, and the residue was purified by silica gel chromatography to give 3- [(2S,4S)-l-tert-butoxycarbony1-4-(1-piperazinyl)-2pyrrolidinylcarbonyl]-l,3-thiazolidine (206 mg) as a white solid.

(3) Using the above-mentioned compound (202 mg) and 1ethoxycarbonyl-4-piperidone (90 μΕ), and in the same manner as

170

CA 02418656 2003-02-06 in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl-4-[4-(1ethoxycarbonyl-4-piperidinyl)-l-piperazinyl]-2- pyrrolidinylcarbonyl}-l,3-thiazolidine (168 mg) was obtained as a white powder.

(4) Using the above-mentioned compound (168 mg), and in the same manner as in Example 133 (2), the title compound (113 mg) was obtained as a white powder.

¹ H-NMR(500MHz,DMSO-de)ôl.18(3H,t,J=7.1Hz), 1.53-1.65(2H,m),

1.81-1.95(lH,m), 2.01-2.21(2H,m), 2.70-4.20(21H,m), 4.04(2H,q,J=7.1Hz), 4.47-4.73(3H,m), 8.89(lH,brs), 10.39(1H,brs), 11.46(lH,brs).

Example 207

Synthesis of 3—((2S,4R)-4-[4-(4-nitrophenyl)-l-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) 3-((2S,4R)-4-Amino-1-tert-butoxycarbony1-2pyrrolidinylcarbonyl)-1,3-thiazolidine (title compound of Reference Example 15, 1 g) was dissolved in N-methyl-2pyrrolidone (20 mL), and N,N-bis{2-[(methylsulfonyl)oxy]ethyl}4-nitroaniline (1.27 g) and Ν,Ν-diisopropylethylamine (1.73 mL) were added thereto. The mixture was stirred at 80°C for 24 hr with heating. The reaction mixture was added to saturated aqueous sodium hydrogencarbonate solution, and the mixture was extracted with ethyl acetate. The extract was washed with brine and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give

3-{(2S,4R)-l-tert-butoxycarbonyl-4-[4-(4-nitrophenyl)-1piperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine.

(2) This was dissolved in methanol (20 mL) and chloroform (10 mL), and 4 mol/L hydrochloric acid-ethyl acetate (10 mL) was added thereto. The mixture was stirred for 2 hr. The reaction mixture was concentrated under reduced pressure, and saturated aqueous sodium hydrogencarbonate solution was added to the residue. The mixture was extracted with chloroform and the

171

CA 02418656 2003-02-06 extract was concentrated under reduced pressure. The residue was purified by silica gel chromatography to give a free base (161 mg) of the title compound. This was dissolved in ethanol (5 mL), and 4 mol/L hydrochloric acid-ethyl acetate (0.21 mL) was added thereto. The solvent was evaporated under reduced pressure to give the title compound (128 mg) as a yellow powder. ¹H-NMR(DMSO-d₅)ô2.22-2.46( lH,m), 2.75-2.99( lH,m), 3.003.20(2H,m), 3.20-4.30(13H,m), 4.40-5.07(3H,m), 7.16(2H,d,J=9.3Hz), 8.12(2H,d,J=9.3Hz).

Example 208

Synthesis of 3-{(2S,4S)-4-(4-(4-trifluoromethylphenyl)-1piperazinyl]-2-pyrrolidinylcarbonyl}-l, 3-thiazolidine tr ihydroc hloride (1) Using the title compound (450 mg) of Reference Example 12 and 1-(4-trifluoromethylphenyl)piperazine (414 mg), and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tertbutoxycarbonyl-4-[4-(4-trifluoromethylphenyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (772 mg) was obtained as a white solid.

(2) The above-mentioned compound (766 mg) was dissolved in methanol (5 mL), and 1.1 mol/L hydrochloric acid-methanol (14 mL) was added thereto. The mixture was stirred at room temperature for 4 days. The reaction mixture was concentrated under reduced pressure, and ethyl acetate was added to the residue. The precipitated solid was collected by filtration to give the title compound (680 mg) as a pale-brown powder.

¹ H-NMR(DMSO-d6)02.27-2.40( lH,m), 2.96-4.16( 16H,m), 4.464.77(3H,m), 7.18(2H,d,J=8.8), 7.58(2H,d,J=8.8), 9.22(lH,brs), 10.73(lH,brs).

Example 209

Synthesis of 3-{(2S,4S)-4-[4-(3-cyano-2-pyridyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl)-l,3-thiazolidine tr ihydrochloride

172

I

CA 02418656 2003-02-06 (1) Piperazine (125 g) was melted by heating at 150°C and 2chloro-3-cyanopyridine (20.0 g) was added thereto. The mixture was stirred at 110°C for 2 hr. Water was added to the reaction mixture and the mixture was extracted with chloroform. The extract was washed with brine, dried and concentrated under reduced pressure to give l-(3-cyano-2-pyridyl)piperazine (24.2 g) as a brown solid.

(2) Using the above-mentioned compound (0.621 g) and the title compound (0.901 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl-

4-(4-(3-cyano-2-pyridyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (1.42 g) was obtained as a white solid.

¹ H-NMR(CDC1₃)01.41(4.5H,s), 1.46(4.5H,S), 1.83-1.97(lH,m),

2.40-2.51(lH,m), 2.53-2.72(4H,m), 2.82-3.22(3H,m),

3.33(1H,t,J=9.9Hz), 3.35-4.14(7H,m), 4.38-4.79(3H,m),

6.76(lH,dd,J=7.6,4.7Hz), 7.77(lH,dd,J=7.6,1.7Hz), 8.34(lH,dd,J=4.7,1.7Hz).

(3) The above-mentioned compound (1.42 g) was dissolved in ethyl acetate (7.5 mL), and 4 mol/L hydrochloric acid-ethyl acetate (7.5 mL) was added thereto. The mixture was stirred at room temperature for 12 hr. The precipitated solid was collected by filtration to give the title compound (1.00 g) as a white powder.

¹ H-NMR(DMSO-d₆)02.26-2.40( lH,m), 2.93-3.18(3H,m), 3.24.8(16H,m), 7.09(lH,dd,J=7.7,4.8), 8.19(lH,dd,J=7.7,1.9), 8.49(lH,dd,J=4.8,1.9), 9.16(lH,brs), 11.02(1H,brs), 12.7(lH,brs).

Example 210

Synthesis of 3-{(2S,4S)-4-[4-(3-chloro-2-pyridyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine tr ihydroc hloride (1) Piperazine (20.0 g) was melted by heating at 140°C and 2,3

173

CA 02418656 2003-02-06 dichloropyridine (3.42 g) was added thereto. The mixture was stirred at 120°C for 2 hr. Water was added to the reaction mixture and the mixture was extracted with chloroform. The extract was washed with brine, dried and concentrated under reduced pressure to give l-(3-chloro-2-pyridyl)piperazine (4.68 g) as a dark-brown oil.

(2) Using the above-mentioned compound (0.712 g) and the title compound (0.901 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-[4-(3-chloro-2-pyridyl)-1-piperazinyl]-2- pyrrolidinylcarbonyl}-1,3-thiazolidine (1.41 g) was obtained as a white solid.

¹ H-NMR(CDC1₃ )01.41(4.5H,s), 1.46(4.5H,s), 1.84-1.99( lH,m),

2.40-2.51(lH,m), 2.53-2.73(4H,m), 2.79-3.18(3H,m), 3.283.45(5H,m), 3.58-4.12(3H,m), 4.38-4.78(3H,m),

6.84(lH,dd,J=7.7,4.7Hz), 7.58(lH,dd,J=7.7,1.5Hz),

8.18(lH,dd,J=4.7,1.5Hz).

(3) The above-mentioned compound (1.40 g) was dissolved in ethanol (4 mL), and 4.1 mol/L hydrochloric acid-ethanol (4 mL) was added thereto. The mixture was stirred at room temperature for 14 hr. The precipitated solid was collected by filtration to give the title compound (1.14 g) as a white powder.

¹ H-NMR (DMSO-de )62.30-2.43 ( lH,m), 2.95-3.18( 3H,m), 3.24.2(13H,m), 4.45-4.80(3H,m), 7.12(lH,dd,J=7.8,4.7Hz), 7.89(lH,dd,J=7.8,1.5Hz), 8.28(lH,dd,J=4.7,1.5Hz), 9.16(lH,brs), 10.96(1H,brs), 12.55(lH,brs).

Example 211

Synthesis of 3-{(2S,4S)-4-[4-(5-ethoxycarbonyl-2-pyridyl)-lpipera z iny1]-2-pyrrolidinylcarbony1}-1,3-thiazolidine tr ihydroc hloride (1) Ethyl 6-chloronicotinate (1.12 g) was dissolved in DMF (30 mL), and 1-tert-butoxycarbonylpiperazine (1.24 g) and potassium carbonate (1.00 g) were added thereto. The mixture was stirred

174

CA 02418656 2003-02-06 at 80°C for 18 hr. Water (100 mL) was added to the reaction mixture and the mixture was extracted with ethyl acetate. The extract was washed successively with saturated aqueous sodium hydrogencarbonate solution and brine and dried. The solvent was evaporated under reduced pressure. The residue was dissolved in dichloromethane (10 mL), and trifluoroacetic acid (5 mL) was added thereto at room temperature. The mixture was stirred for 2 hr and the reaction mixture was concentrated under reduced pressure. Water (50 mL) was added to the residue, and the mixture was washed with diethyl ether. The aqueous layer was basified with aqueous sodium hydrogen carbonate solution. The mixture was extracted with chloroform. The extract was dried and the mixture was concentrated under reduced pressure to give l-(5-ethoxycarbonyl-2pyridyl)piperazine (1.17 g) as an oil.

(2) Using the above-mentioned compound (1.17 g) and the title compound (1.47 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-[4-(5-ethoxycarbonyl-2-pyridyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (2.07 g) as a white solid.

(3) Using the above-mentioned compound (1.06 g), and in the same manner as in Example 133 (2), the title compound (1.06 g) was obtained as a white powder.

¹ H-NMR(500MHz,DMSO-de)01.30(3H,t,J=7.1Hz), 2.23-2.43(lH,m), 2.92-4.90(19H,m), 4.28(2H,q,J=7.1Hz), 7.05(lH,d,J=9.1Hz), 8.04(lH,dd,J=9.1,2.3Hz), 8.69(lH,d,J=2.3Hz), 9.13(lH,brs), 10.91(lH,brs), 12.58(lH,brs).

Example 212

Synthesis of 3-{(2S,4S)-4-[4-(5-carboxy-2-pyridyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride mol/L Hydrochloric acid was added to 3-{(2S,4S)-l-tert

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CA 02418656 2003-02-06 butoxycarbonyl-4-[4-(5-ethoxycarbonyl-2-pyridyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride [product of Example 211 (2), 1.00 g] and the mixture was refluxed for 2 hr. The solvent was evaporated under reduced pressure, and the residue was purified by HPLC. This was converted to hydrochloride with 4 mol/L hydrochloric acid-l,4-dioxane to give the title compound (158 mg) as a white powder.

¹ H-NMR(500MHz,DMSO-d₆)02.21-2.41(lH,m), 2.90-4.90(19H,m),

7.04(lH,d,J=9.0Hz), 8.03(lH,dd,J=9.0,2.2Hz), 8.67(lH,d,J=2.2Hz), 9.12(lH,brs), 10.80(lH,brs), 12.50(lH,brs).

Example 213

Synthesis of 3-{(2S,4S)-4-[4-(5-carbamoyl-2-pyridyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-1,3-thiazolidine trihydrochloride (1) Using 6-chloronicotinamide (5.00 g) and piperazine (27.6 g), and by reaction in the same manner as in Example 196 (1) at 100°C, l-(5-carbamoyl-2-pyridyl)piperazine (0.41 g) was obtained as a yellow powder.

(2) Using the above-mentioned compound (370 mg) and the title compound (450 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-[4-(5-carbamoyl-2-pyridyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (350 mg) was obtained as a white solid.

(3) Using the above-mentioned compound (347 mg), and in the same manner as in Example 133 (2), the title compound (332 mg) was obtained as a white powder.

¹ H-NMR(500MHz,DMSO-de)61.81-2.01(lH,m), 2.93-4.10(14H,m), 4.284.75(5H,m), 7.08(lH,d,J=9.0Hz), 7.26(lH,brs), 7.91(lH,brs), 8.10(lH,dd,J=9.0,2.3Hz), 8.66(lH,d,J=2.3Hz), 9.12(lH,brs), 10.87(lH,brs), 12.51(lH,brs).

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Example 214

Synthesis of 3-{(2S,4R)-4-[4-(5-cyano-2-pyridyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-1,3-thiazolidine tr ihydroc hloride (1) The title compound (1.74 g) of Reference Example 11 and triethylamine (1.0 mL) were dissolved in dichloromethane (35 mL), and methanesulfonyl chloride (0.49 mL) was added dropwise under ice-cooling. The mixture was stirred for 3 hr. Iced water was added to the reaction mixture and the mixture was extracted with dichloromethane. The extract was washed with brine, dried and concentrated under reduced pressure to give 3{(2S,4S)-l-tert-butoxycarbonyl-4-methanesulfonyloxy-2pyrrolidinylcarbonyl}-l,3-thiazolidine (2.03 g) as a pale-brown solid.

(2) The above-mentioned compound (1.10 g) and 1-(5-cyano-2pyridyl)piperazine (1.12 g) were dissolved in l-methyl-2pyrrolidone (20 mL), and the mixture was stirred at 90°C for 12 hr. The reaction mixture was added to water and the mixture was extracted with ethyl acetate. The extract was concentrated under reduced pressure and the residue was purified by column chromatography to give 3-{(2S,4R)-l-tert-butoxycarbony1-4-(4- (5-cyano-2-pyridyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-l,3thiazolidine (170 mg).

¹ H-NMR(CDCl₃)01.41(4.5H,s), 1.46( 4.5H, s), 2.07-2.20(2H,m),

2.45-2.65(4H,m), 3.00-3.40(4H,m), 3.57-3.79(5H,m), 3.814.00(2H,m), 4.45-4.83(3H,m), 6.59(lH,d,J=9.3Hz), 7.61(lH,dd,J=9.3,1.9Hz), 8.40(lH,d,J=1.9Hz).

(3) The above-mentioned compound (150 mg) was suspended in tetrahydrofuran (40 mL), and 4 mol/L hydrochloric acid-ethyl acetate (40 mL) was added thereto. The mixture was stirred at 55°C for 5 hr. After allowing to cool, the precipitated solid was collected by filtration to give the title compound (130 mg) as a white powder.

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CA 02418656 2003-02-06 ¹ H-NMR(DMSO-d₆)ô2.26-2.45( lH,m), 2.80-3.00(lH,m), 3.013.27(3H,m), 3.28-3.94(8H,m), 3.95-4.15(3H,m), 4.38-4.77(3H,m),

4.84-5.01(lH,m), 7.12(lH,d,J=9.3Hz), 8.00(lH,dd,J=9.3,2.1Hz),

8.58(lH,d,J=2.1Hz), 9.22(1H,brs).

Example 215

Synthesis of 3-{(2S,4S)-4-(4-(3-chloro-5-trifluoromethy1-2pyridyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-l,3thiazolidine trihydrochloride (1) Piperazine (40 g) was melted by heating at 140°C and 2,3dichloro-5-trifluoromethylpyridine (10 g) was added thereto. The mixture was stirred at 120°C for 2 hr. Water was added to the reaction mixture and the mixture was extracted with chloroform. The extract was washed with brine, dried and concentrated under reduced pressure to give l-(3-chloro-5trifluoromethyl-2-pyridyl)piperazine (12.8 g) as a brown solid.

(2) Using the above-mentioned compound (0.956 g) and the title compound (0.901 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(3-chloro-5-trifluoromethyl-2-pyridyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (1.64 g) was obtained as a white solid.

¹ H-NMR(CDCl₃)ôl.41(4.5H,s), 1.46(4.5H,s), 1.83-1.98( lH,m),

2.38-2.50(lH,m), 2.52-2.70(4H,m), 2.78-3.19(3H,m),

3.33(1H,t,J=10.1Hz), 3.47-4.13(7H,m), 4.37-4.81(3H,m), 7.75(1H,S), 8.38(1H,S).

(3) The above-mentioned compound (1.64 g) was dissolved in ethanol (4 mL), and 4.1 mol/L hydrochloric acid-ethanol (4 mL) was added thereto. The mixture was stirred at room temperature for 5 days. The precipitated solid was collected by filtration to give the title compound (1.20 g) as a white powder.

¹ H-NMR(DMSO-d6)02.30-2.47( lH,m), 2.92-4.2( 16H,m), 4.454.78(3H,m), 8.31(lH,d,J=1.9Hz), 8.63(lH,d,J=1.9Hz), 9.15(lH,brs), 10.77(1H,brs), 12.6(lH,brs).

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Example 216

Synthesis of 3-{(2S,4S)-4-[4-(3-chloro-5-ethoxycarbonyl-2pyridyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-l,3thiazolidine trihydrochloride (1) 5,6-Dichloronicotinic acid (4.90 g) was dissolved in ethanol (40 mL), and thionyl chloride (2.0 mL) was added thereto under ice-cooling. The mixture was refluxed for 1.5 hr. The reaction mixture was concentrated under reduced pressure, and saturated aqueous sodium hydrogencarbonate solution was added to the residue. The mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give ethyl 5,6-dichloronicotinate (4.85 g) as a white solid.

(2) Piperazine (19.0 g) was melted by heating at 140°C and the above-mentioned compound (4.80 g) was added thereto. The mixture was stirred at 120°C for 2 hr. Water was added to the reaction mixture and the mixture was extracted with chloroform. The extract was washed with brine, dried and concentrated under reduced pressure to give l-(3-chloro-5-ethoxycarbonyl-2pyridyl)piperazine (5.64 g) as a dark brown solid.

(3) Using the above-mentioned compound (5.12 g) and the title compound (4.75 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(3-chloro-5-ethoxycarbonyl-2-pyridyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (7.53 g) was obtained as a white solid.

¹ H-NMR(CDCl₃)ôl.38 (3H, t, J=7.1Hz), 1.41 ( 4.5H, S), 1.46( 4.5H, S) ,

1.83-1.98(lH,m), 2.38-2.50(lH,m), 2.53-2.73(4H,m), 2.783.20(3H,m), 3.33(lH,t,J=10.3Hz), 3.45-4.13(7H,m),

4.36(2H,q,J=7.1Hz), 4.32-4.78(3H,m), 8.11(lH,d,J=1.8Hz), 8.74(lH,d,J=1.8Hz).

(4) The above-mentioned compound (1.00 g) was dissolved in

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CA 02418656 2003-02-06 dichloroethane (10 mL) and trifluoroacetic acid (5 mL) was added thereto. The mixture was stirred at room temperature for 3 hr. The reaction mixture was concentrated under reduced pressure, and the residue was dissolved in ethyl acetate (10 mL). 4 mol/L Hydrochloric acid-ethyl acetate (2.25 mL) was added thereto, and the mixture was stirred at room temperature for 1 hr. The precipitated solid was collected by filtration to give the title compound (0.82 g) as a white powder.

¹ H-NMR(DMSO-d₆) 01.32( 3H,t,J=7.1Hz), 2.26-2.40( lH,m), 2.933.18(3H,m), 3.25-4.15(13H,m), 4.32(2H,q,J=7.1Hz), 4.44.78(3H,m), 8.19(lH,d,J=2.0Hz), 8.73(lH,d,J=2.0Hz), 9.16(lH,brs), 10.78(1H,brs), 12.5(lH,brs).

Example 217

Synthesis of 3-{(2S,4S)-4-(4-(5-carboxy-3-chloro-2-pyridyl)-1piperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) 3-{(2S,4S)-l-tert-Butoxycarbonyl-4-(4-(3-chloro-5ethoxycarbony1-2-pyridyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine [product of Example 216 (3), 6.49 g] was dissolved in ethanol (30 mL), and aqueous solution (30 mL) of lithium hydroxide (0.59 g) was added thereto. The mixture was stirred at room temperature for 19 hr and ethanol was evaporated under reduced pressure. The concentrate was washed with ethyl acetate, and 1 mol/L hydrochloric acid was added thereto. The precipitated solid was collected by filtration to give 3-{(2S,4S)-l-tertbutoxycarbonyl-4- [4-(5-carboxy-3-chloro-2-pyridyl)-1piperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine (2.64 g) as a white solid.

¹ H-NMR ( DMSO-de )01.33( 4.5H, s), 1.41(4.5H,S), 1.97-2.15 ( lH,m),

2.77-2.87(lH,m), 2.95-4.25(15H,m), 4.40-4.76(3H,m),

8.16(lH,d,J=2.0Hz), 8.71(lH,d,J=2.0Hz), 11.40(1H,brs).

(2) Using the above-mentioned compound (500 mg), and in the

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CA 02418656 2003-02-06 same manner as in Example 216 (4), the title compound (437 mg) was obtained as a white powder.

¹ H-NMR(DMSO-d₆)02.27-2.42( lH,m), 2.94-3.18(3H,m), 3.24.8(16H,m), 8.17(lH,d,J=2.0Hz), 8.71(lH,d,J=2.0Hz), 9.16(lH,brs), 10.95(lH,brs), 12.60(lH,brs)

Example 218

Synthesis of 3-{(2S,4S)-4-[4-(5-carbamoyl-3-chloro-2-pyridyl)-

1- piperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) 3-{(2S,4S)-1-tert-Butoxycarbony1-4-(4-(5-carboxy-3-chloro-

2- pyridyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-1,3thiazolidine [product of Example 217 (1), 2.63 g] and ammonium chloride (0.54 g) were dissolved in DMF (30 mL), and Nmethylmorpholine (1.1 mL), HOBT (1.53 g) and EDC hydrochloride (1.15 g) were successively added thereto. The mixture was stirred at room temperature for 4 hr. The reaction mixture was concentrated under reduced pressure, and saturated aqueous sodium hydrogencarbonate solution was added to the residue. The mixture was extracted with ethyl acetate. The extract washed with brine and dried. The solvent was evaporated under reduced pressure to give 3-{(2S,4S)-l-tert-butoxycarbonyl-4-[4(5-carbamoyl-3-chloro-2-pyr idyl)-1-pipera z iny1]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (2.50 g) as a white solid.

¹ H-NMR(CDCl₃)ôl.41(4.5H,s), 1.45(4.5H,s), 1.84-1.98(lH,m),

2.39-2.52(lH,m), 2.53-2.73(4H,m), 2.78-3.18(3H,m), 3.34(lH,t,J=10.0Hz), 3.45-4.13(7H,m), 4.38-4.78(3H,m), 5.87(lH,brs), 6.17(lH,brs), 8.07(lH,d,J=2.0Hz), 8.56(lH,d,J=2.0Hz).

(2) The above-mentioned compound (683 mg) was dissolved in ethanol (2 mL), and 4.1 mol/L hydrochloric acid-ethanol (2 mL) was added thereto. The mixture was stirred at room temperature for 22 hr. The precipitated solid was collected by filtration

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CA 02418656 2003-02-06 to give the title compound (616 mg) as a white powder.

¹H-NMR(DMSO-de )62.26-2.42 ( lH,m), 2.92-4.3 ( 16H,m), 4.464.78(3H,m), 7.56(lH,brs), 8.12(lH,brs), 8.26(lH,s), 8.73(lH,s), 9.15(lH,brs), 10.83(lH,brs), 12.52(lH,brs).

Example 219

Synthesis of 3-{(2S,4S)-4-[4-(3-chloro-5-cyano-2-pyridyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-1,3-thiazolidine trihydrochloride (1) 3-{(2S,4S)-l-tert-Butoxycarbonyl-4-[4-(5-carbamoyl-3chloro-2-pyridyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-1,3thiazolidine [product of Example 218 (1), 1000 mg] and imidazole (195 mg) were dissolved in pyridine (10 mL), and phosphorus oxychloride (0.35 mL) was added thereto under icecooling. The mixture was stirred at room temperature for 13 hr and saturated aqueous sodium hydrogencarbonate solution was added to the reaction mixture. The mixture was extracted with chloroform. The extract was washed with brine, dried and concentrated under reduced pressure. The residue was purified by silica gel chromatography to give 3-{(2S,4S)-l-tertbutoxycarbonyl-4- [ 4-( 3-chloro-5-cyano-2-2 -pyridyl )-1piperazinyl]-2-pyrrolidinylcarbonyl}-1,3-thiazolidine (628 mg) as a white solid.

¹ H-NMR(CDC13)01.41(4.5H,s), 1.45(4.5H,S), 1.83-1.98( lH,m),

2.38-2.70(5H,m), 2.80-3.20(3H,m), 3.33(lH,t,J=10.0Hz), 3.534.13(7H,m), 4.37-4.82(3H,m), 7.73(lH,d,J=1.9Hz), 8.37(lH,d,J=1.9Hz).

(2) The above-mentioned compound (622 mg) was dissolved in ethyl acetate (1.5 mL), and 4 mol/L hydrochloric acid-ethyl acetate (1.5 mL) was added thereto. The mixture was stirred at room temperature for 14 hr. The reaction mixture was concentrated under reduced pressure, and saturated aqueous sodium hydrogencarbonate solution was added to the residue. The mixture was extracted with chloroform. The extract was

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This was dissolved in ethyl acetate (6 mL) and 4 mol/L hydrochloric acid-ethyl acetate (0.9 mL) was added thereto.

The mixture was stirred at room temperature for 1 hr. The precipitated solid was collected by filtration to give the title compound (388 mg) as a white powder.

¹ H-NMR (DMSO-de )62.10-2.26 ( lH,m), 2.90-4.2 ( 16H,m), 4.474.78(3H,m), 8.41(lH,d,J=1.9Hz), 8.69(lH,d,J=l.9Hz), io 9.13(lH,brs), 10.81(lH,brs), 12.57(lH,brs).

Example 220

Synthesis of 3-{(2S,4S)-4-(4-(3,5-dichloro-2-pyridyl)-1pipera z iny1]-2-pyrrolidinylcarbony1}-1,3-thiazolidine tr ihydrochloride

183

CA 02418656 2003-02-06 (1) Piperazine (24.0 g) was melted by heating at 140°C and

2,3,5-trichloropyridine (5.00 g) was added thereto. The mixture was stirred at 120°C for 2 hr. Water was added to the reaction mixture and the mixture was extracted with chloroform. The extract was washed with brine, dried and concentrated under reduced pressure to give 1-(3,5-dichloro-2-pyridyl)piperazine (6.43 g) as a brown solid.

(2) Using the above-mentioned compound (0.832 g) and the title compound (0.901 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(3,5-dichloro-2-pyridyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (1.35 g) was obtained as a white solid.

¹ H-NMR(CDC1₃)01.41 ( 4.5H, s) , 1.46(4.5H,s), 1.82-1.98( lH,m),

2.38-2.50(lH,m), 2.53-2.73(4H,m), 2.78-3.22(3H,m), 3.283.44(5H,m), 3.62-4.14(3H,m), 4.38-4.80(3H,m),

7.59(lH,d,J=2.2Hz), 8.12(lH,d,J=2.2Hz).

(3) The above-mentioned compound (1.34 g) was dissolved in ethanol (3.5 mL), and 4.1 mol/L hydrochloric acid-ethanol (3.5 mL) was added thereto. The mixture was stirred at room temperature for 15 hr. The precipitated solid was collected by filtration to give the title compound (1.10 g) as a white powder.

¹ H-NMR (DMSO-de )62.20-2.38( lH,m), 2.90-4.15 ( 16H,m), 4.464.78(3H,m), 8.15(lH,d,J=2.3Hz), 8.73(lH,d,J=2.3Hz), 9.13(lH,brs), 10.84(lH,brs), 12.55(lH,brs).

Example 221

Synthesis of 3-{(2S,4S)-4-[4-(3,5-dichloro-4-pyridyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) Using 1-(3,5-dichloro-4-pyridyl)piperazine (0.766 g) and the title compound (0.901 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert184

CA 02418656 2003-02-06 butoxycarbonyl-4-[4-(3,5-dichloro-4-pyridyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (1.53 g) was obtained as a white solid.

¹ H-NMR(CDCl₃)01.41(4.5H,s) , 1.46(4.5H, s), 1.82-1.98( lH,m),

2.40-2.52(lH,m), 2.55-2.72(4H,m), 2.83-3.21(3H,m), 3.283.45(5H,m), 3.62-4.14(3H,m), 4.38-4.81(3H,m), 8.33(2H,s).

(2) The above-mentioned compound (1.53 g) was dissolved in ethyl acetate (7.5 mL), and 4 mol/L hydrochloric acid-ethyl acetate (7.5 mL) was added thereto. The mixture was stirred at room temperature for 13 hr. The precipitated solid was collected by filtration to give the title compound (1.64 g) as a white powder.

¹ H-NMR(DMSO-d₆)02.27-2.43( lH,m), 2.95-4.2 ( 16H,m), 4.454.78(3H,m), 8.52(2H,s), 9.14(lH,brs), 10.97(lH,brs), 12.6(lH,brs).

Example 222

Synthesis of 3-{(2S,4S)-4-[4-(3-methyl-l-phenyl-5-pyrazolyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) 1-tert-Butoxycarbonylpiperazine (5.02 g) was dissolved in DMF (90 mL), and diketene (2.50 mL) was added thereto at room temperature. The mixture was stirred for 1.5 hr and the solvent was evaporated under reduced pressure. Water was added to the residue, and the mixture was extracted with ethyl acetate. The extract was washed with brine and dried. The solvent was evaporated under reduced pressure to give 1acetoacetyl-4-tert-butoxycarbonylpiperazine (6.26 g) as a palebrown powder.

(2) The above-mentioned compound (6.24 g) was dissolved in ethanol (500 mL), and phenylhydrazine (2.27 mL) and methanesulfonic acid (350 μΕ) were added at room temperature. The mixture was stirred for 14 hr and pyridine (6 mL) was added to the reaction mixture. The solvent was evaporated under

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CA 02418656 2003-02-06 reduced pressure and the residue was dissolved in pyridine (250 mL). Phosphorus oxychloride (5.0 mL) was added thereto at room temperature and the mixture was stirred for 20 hr. The solvent was evaporated under reduced pressure, the residue was acidified with dilute hydrochloric acid to pH 3. The mixture was extracted with ethyl acetate. The extract was washed successively with saturated aqueous sodium hydrogencarbonate solution and brine and dried. The solvent was evaporated under reduced pressure and the residue was purified by silica gel chromatography to give l-tert-butoxycarbonyl-4-(3-methy1-1phenyl-5-pyrazolyl)piperazine (935 mg) as an oil.

(3) The above-mentioned compound (935 mg) was dissolved in dichloromethane (10 mL), and trifluoroacetic acid (5 mL) was added thereto at room temperature. The mixture was stirred for

1.5 hr. The solvent was evaporated under reduced pressure, and water (50 mL) was added to the residue. The mixture was washed with diethyl ether. The aqueous layer was basified with aqueous sodium hydrogen carbonate solution, and the mixture was extracted with chloroform. The extract was dried, and evaporated under reduced pressure to give l-(3-methyl-l-phenyl-

5-pyrazolyl)piperazine (584 mg) as a brown powder.

(4) Using the above-mentioned compound (584 mg) and the title compound (604 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-&lt;(2S,4S)-1-tert-butoxycarbony14-(4-(3-methyl-l-phenyl-5-pyrazolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (846 mg) was obtained as a pale-yellow powder.

(5) Using the above-mentioned compound (844 mg), and in the same manner as in Example 133 (2), the title compound (751 mg) was obtained as a white solid.

¹ H-NMR(500MHz,DMSO-de)62.17(3H,S), 2.18-2.38(lH,m), 2.904.10(16H,m), 4.46-4.74(3H,m), 5.93(lH,s), 7.31(lH,m), 7.47(2H,m), 7.79(2H,m), 9.09(lH,brs), 10.91(1H,brs),

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12.40(lH,brs).

Example 223

Synthesis of 3-{(2S,4S)-4-(4-(l-tert-butyl-3-methy1-5pyrazolyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-l,3thiazolidine trihydrochloride (1) l-Acetoacetyl-4-tert-butoxycarbonylpiperazine [product of Example 222 (1), 3.92 g] was dissolved in ethanol (300 mL), and tert-butylhydrazine hydrochloride (1.81 g) and molecular sieves 3A (10 g) were added thereto at room temperature. The mixture was stirred for 15 hr. The reaction mixture was filtrated and the filtrate was concentrated under reduced pressure. The residue was dissolved in pyridine (200 mL), and phosphorus oxychloride (3.0 mL) was added thereto at room temperature. The mixture was stirred for 24 hr. The reaction mixture was concentrated under reduced pressure, the residue was acidified with dilute hydrochloric acid to pH 3. The mixture was extracted with ethyl acetate. The extract was washed successively with saturated aqueous sodium hydrogencarbonate solution and brine and dried. The solvent was evaporated under reduced pressure and the residue was purified by silica gel chromatography to give l-tert-butoxycarbonyl-4-(l-tert-butyl-3methyl-5-pyrazolyl)piperazine (886 mg) as an oil.

(2) Using the above-mentioned compound (880 mg), and in the same manner as in Example 222 (3), 1-(3-methyl-l-tert-butyl-5pyrazolyl)piperazine (607 mg) was obtained as a pale-yellow powder.

(3) Using the above-mentioned compound (0.607 g) and the title compound (0.781 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(3-methyl-l-tert-butyl-5-pyrazolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (1.17 g) was obtained as a white powder.

(4) Using the above-mentioned compound (1.17 g), and in the

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CA 02418656 2003-02-06 same manner as in Example 133 (2), the title compound (0.902 g) was obtained as a white solid.

¹ H-NMR(500MHz,DMSO-de)61.56(9H,S), 2.09(3H,S), 2.27-2.47(lH,m),

2.90-4.20(16H,m), 4.48-4.78(3H,m), 6.00(lH,s), 9.12(lH,brs), 11.12(1H,brs), 12.49(lH,brs).

Example 224

Synthesis of 3-{(2S,4S)-4-[4-(l-phenyl-2-imidazolyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) 1-Benzyloxycarbonylpiperazine (5.00 g) was dissolved in acetone (50 mL), and phenyl isothiocyanate (2.9 mL) was added thereto under ice-cooling. The mixture was stirred at room temperature for 1 hr. The precipitated solid was collected by filtration to give l-(anilinocarbothioyl)-4- (benzyloxycarbonyl)piperazine (5.08 g) as a white powder.

(2) The above-mentioned compound (5.07 g) was dissolved in methanol (100 mL) and dichloromethane (20 mL), methyl iodide (1.4 mL) were added thereto. The mixture was stirred at room temperature for 17 hr. The reaction mixture was concentrated under reduced pressure, and saturated aqueous sodium hydrogencarbonate solution was added to the residue. The mixture was extracted with dichloromethane, and the extract was washed with brine, dried and concentrated under reduced pressure to give l-benzyloxycarbonyl-4- [(methylthio)phenyliminomethyl]piperazine (5.71 g) as a slightly yellow oil.

(3) The above-mentioned compound (3.00 g) and aminoacetaldehyde dimethyl acetal (1.8 mL) were dissolved in pyridine (15 mL), and the mixture was stirred at 100°C for 2 days with heating. The reaction mixture was concentrated under reduced pressure, and saturated aqueous sodium hydrogencarbonate solution was added to the residue. The mixture was extracted with chloroform and the extract was washed with brine, dried and

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CA 02418656 2003-02-06 concentrated under reduced pressure. The residue was dissolved in 2 mol/L hydrochloric acid (30 mL), and heated at 100°C for 2 hr. Saturated aqueous sodium hydrogencarbonate solution was added to the reaction mixture, and the mixture was extracted with chloroform. The extract was washed with brine, dried and concentrated under reduced pressure. The residue was purified by silica gel chromatography to give l-benzyloxycarbonyl-4-(1phenyl-2-imidazolyl)piperazine (1.16 g) as a brown oil.

(4) The above-mentioned compound (1.16 g) was dissolved in methanol (30 mL). The solution was stirred in the presence of 10% palladium/carbon (232 mg) under a hydrogen atomosphere (1 atm) at room temperature for 20 hr. The reaction mixture was filtrated and the filtrate was concentrated under reduced pressure to give 1-(l-phenyl-2-imidazolyl)piperazine (0.742 g) as a white solid.

(5) Using the above-mentioned compound (0.740 g) and the title compound (0.901 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(1-pheny1-2-imidazolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (1.30 g) was obtained as a white solid.

¹ H-NMR(CDC1₃)61.39(4.5H,s), 1.44(4.5H, s), 1.75-1.92( lH,m),

2.36-2.57(5H,m), 2.74-2.89(lH,m), 2.93-3.17(6H,m),

3.25(lH,t,J=10.0Hz), 3.60-4.08(3H,m), 4.34-4.77(3H,m), 6.836.88(2H,m), 7.34(1H,t,J=7.1Hz), 7.43-7.54(4H,m).

(6) The above-mentioned compound (1.30 g) was dissolved in ethanol (3 mL), and 4.1 mol/L hydrochloric acid-ethanol (3 mL) was added thereto. The mixture was stirred at room temperature for 18 hr. The precipitated solid was collected by filtration to give the title compound (1.15 g) as a white powder.

¹ H-NMR(DMSO-de)62.10-2.26( lH,m), 2.83-4.05( 16H,m), 4.434.77(3H,m), 7.48(lH,d,J=2.3Hz), 7.53(lH,d,J=2.3Hz), 7.547.72(5H,m), 9.07(lH,brs), 10.98(lH,brs).

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Example 225

Synthesis of 3-{(2S,4S)-4-[4-(5-methyl-l-phenyl-2-imidazolyl)l-piperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) l-Benzyloxycarbonyl-4- [(methylthio)phenyliminomethyl]piperazine [product of Example 224 (2), 2.70 g] and propargylamine (2.3 mL) were dissolved in 1-butanol (25 mL), and p-toluenesulfonic acid monohydrate (156 mg) was added thereto. The mixture was refluxed for 20 hr. The reaction mixture was concentrated under reduced pressure, and saturated aqueous sodium hydrogencarbonate solution was added to the residue. The mixture was extracted with chloroform, and the extract was washed with brine, dried and concentrated under reduced pressure. The residue was purified by silica gel chromatography to give 1-benzyloxycarbony1-4-(5methyl-l-phenyl-2-imidazolyl)piperazine (1.82 g) as a brown oil.

(2) Using the above-mentioned compound (1.16 g), and in the same manner as in Example 224 (4), l-(5-methyl-l-phenyl-2imidazolyl)piperazine (1.23 g) was obtained as a brown solid.

(3) Using the above-mentioned compound (0.800 g) and the title compound (0.901 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-[4-(5-methyl-l-phenyl-2-imidazolyl)-l-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (1.20 g) was obtained as a white solid.

¹ H-NMR(CDC1₃)ôl.38(4.5H,s), 1.43(4.5H,S), 1.75-1.88( lH,m), 1.99(3H,s), 2.28-2.46(5H,m), 2.68-2.83(lH,m), 2.90-3.16(6H,m), 3.22(lH,t,J=10.1Hz), 3.57-4.07(3H,m), 4.32-4.75(3H,m), 6.57(lH,s), 7.27-7.53(5H,m).

(4) The above-mentioned compound (1.19 g) was dissolved in ethanol (3 mL), and 4.1 mol/L hydrochloric acid-ethanol (3 mL) was added thereto. The mixture was stirred at room temperature for 13 hr. The precipitated solid was collected by filtration

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CA 02418656 2003-02-06 to give the title compound (0.913 g) as a white powder.

¹ H-NMR(DMSO-de)02.00-2.18(lH,m), 2.79-3.93( 16H,m), 4.424.76(3H,m), 7.23(lH,s), 7.58-7.72(5H,m), 9.02(lH,brs), 10.86(lH,brs), 14.01(1H,brs).

Example 226

Synthesis of 3-{(2S,4S)-4-[4-(4-phenyl-2-thiazolyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-1,3-thiazolidine trihydrochloride (1) Phenacyl bromide (4 g) was dissolved in acetonitrile (30 mL), and sodium thiocyanate (1.8 g) was added at room temperature with stirring. The reaction mixture was concentrated under reduced pressure, and brine was added to the residue. The mixture was extracted with ethyl acetate. The extract was dried, and the solvent was evaporated under reduced pressure to give 2-isocyanatoacetophenone (3.53 g) as white crystals.

(2) Piperazine (3.8 g) was dissolved in ethanol (40 mL), and a solution of the above-mentioned compound (3.53 g) in ethyl acetate (10 mL) was added thereto. The mixture was heated at 70°C for 1 hr. The reaction mixture was concentrated under reduced pressure, and brine was added to the residue. The mixture was extracted with ethyl acetate, and the extract was dried and concentrated under reduced pressure. The residue was purified by silica gel chromatography to give 4-phenyl-2-(1piperazinyl)thiazole (2.38 g) as a yellow oil.

(3) Using the above-mentioned compound (0.810 g) and the title compound (0.901 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(4-phenyl-2-thiazolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (1.59 g) was obtained as a pale-yellow solid.

¹H-NMR(CDCl₃)ôl.41(4.5H,S) , 1.46(4.5H,s), 1.84-1.98( lH,m),

2.40-2.72(5H,m), 2.80-3.18(3H,m), 3.34(lH,t,J=9.9Hz), 3.49191

CA 02418656 2003-02-06

4.15(7H,m), 4.38-4.80(3H,m), 6.78(lH,s), 7.26-7.46(13H,m),

7.83(2H,d,J=7.1Hz).

(4) The above-mentioned compound (1.59 g) was dissolved in ethanol (6 mL), and 4.1 mol/L hydrochloric acid-ethanol (6 mL) was added thereto. The mixture was stirred at room temperature for 12 hr. The precipitated solid was collected by filtration to give the title compound (1.41 g) as a pale-brown powder.

¹ H-NMR(DMSO-dg)Ô2.27-2.42( lH,m), 2.95-3.18(3H,m), 3.374.18(16H,m), 4.47-4.78(3H,m), 7.30(lH,t,J=7.3Hz), 7.377.45(3H,s), 7.87(2H,d,J=7.1Hz), 9.17(lH,brs), 10.93(1H,brs).

Example 227

Synthesis of 3-((2S,4S)-4-{4-[4-(4-cyanophenyl)-2-thiazolyl]-lpiperazinyl}-2-pyrrolidinylcarbonyl)-1,3-thiazolidine tr ihydroc hloride (1) 4-Acetylbenzonitrile (4.35 g) was dissolved in chloroform (40 mL), and a solution of bromine (1.7 mL) in chloroform (10 mL) was added dropwise. The mixture was stirred at room temperature. The reaction mixture was washed with saturated aqueous sodium hydrogencarbonate solution, dried and concentrated under reduced pressure. The residue was dissolved in acetonitrile (40 mL), and sodium thiocyanate (2.4 g) was added thereto. The mixture was stirred at room temperature and concentrated under reduced pressure. Brine was added to the residue, and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by silica gel chromatography to give 4-(2-isocyanatoacetyl)benzonitrile (4.39 g) as yellow crystals.

(2) The above-mentioned compound (4.39 g) and piperazine (4.15 g) were dissolved in ethanol (70 mL), and the mixture was stirred at 80°C for 1 hr with heating. The reaction mixture was concentrated under reduced pressure, and brine was added to the residue. The mixture was extracted with ethyl acetate.

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The extract was dried and concentrated under reduced pressure.

The residue was purified by silica gel chromatography to give

1-(4-(4-cyanophenyl)-2-thiazolyl]piperazine (1.83 g) as a yellow solid.

(3) Using the above-mentioned compound (0.892 g) and the title compound (0.901 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-((2S,4S)-l-tert-butoxycarbonyl4-(4-(4-(4-cyanophenyl)-2-thiazolyl]-l-piperazinyl}-2pyrrolidinylcarbonyl)-1,3-thiazolidine (1.40 g) was obtained as a brown solid.

¹ H-NMR(CDCl₃)ôl.41(4.5H,S), 1.46(4.5H,S), 1.83-1.98( lH,m),

2.38-2.75(5H,m), 2.82-3.22(3H,m), 3.34(lH,t,J=9.9Hz), 3.484.15(7H,m), 4.38-4.81(3H,m), 6.93(lH,s), 7.65(2H,d,J=8.3Hz), 8.34(2H,d,J=8.3Hz).

(4) The above-mentioned compound (1.39 g) was dissolved in ethyl acetate (3 mL), and 4 mol/L hydrochloric acid-ethyl acetate (6 mL) was added thereto. The mixture was stirred at room temperature for 4 days. The precipitated solid was collected by filtration to give the title compound (1.23 g) as a pale-yellow powder.

¹ H-NMR(DMSO-ds) Ô2.24-2.38( lH,m), 2.94-3.18(3H,m), 3.354.14(16H,m), 4.46-4.78(3H,m), 7.73(lH,s), 7.87(2H,d,J=8.5Hz), 8.07(2H,d,J=8.5Hz), 9.16(lH,brs), 10.83(lH,brs).

Example 228

Synthesis of 3-((2S,4S)-4-[4-(l-phenyl-lH-tetrazol-5-yl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) Using 5-chloro-l-phenyl-lH-tetrazol (2.10 g) and piperazine (10.0 g), and reaction at 100°C in the same manner as in Example 196 (1), 1-(l-phenyl-lH-tetrazol-5-yl)piperazine (2.67 g) was obtained as a pale-yellow powder.

(2) Using the above-mentioned compound (0.59 g) and the title compound (0.696 g) of Reference Example 12, and in the same

193

CA 02418656 2003-02-06 manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(l-phenyl-lH-tetrazol-5-yl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (1.19 g) was obtained as a white powder.

(3) Using the above-mentioned compound (1.19 g), and in the same manner as in Example 133 (2), the title compound (0.863 g) was obtained as a white powder.

¹ H-NMR(500MHz,DMSO-de)02.02-2.22(lH,m), 2.80-3.95(16H,m), 4.454.73(3H,m), 7.57-7.73(5H,m), 9.04(lH,brs), 10.61(lH,brs).

Example 229

Synthesis of 3-{(2S,4S)-4-[4-(l-cyclohexyl-lH-tetrazol-5-yl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) 1-Benzyloxycarbonylpiperazine (2.07 g) was dissolved in dichloromethane (50 mL), and cyclohexyl isocyanate (1.20 mL) was added thereto at room temperature. The mixture was stirred for 1 hr. The solvent was evaporated under reduced pressure, and the residue was dissolved in tetrahydrofuran (100 mL). Phosphorus oxychloride (8.8 mL) was added thereto, and the mixture was refluxed for 18 hr. The reaction mixture was concentrated under reduced pressure, and a 0.5 mol/L solution of triazole in acetonitrile (100 mL) was added to the residue. The mixture was stirred at room temperature for 3 hr. The solvent was evaporated under reduced pressure, and saturated aqueous sodium hydrogencarbonate solution was added to the residue. The mixture was extracted with ethyl acetate, and the extract was washed with brine and dried. The solvent was distilled away under reduced pressure, and the residue was dissolved in methanol (100 mL). An aqueous solution (20 mL) of sodium azide (6.50 g) was added thereto. The mixture was stirred at 70°C for 3 hr and the reaction mixture was concentrated under reduced pressure. Water was added to the residue, and the mixture was extracted with ethyl acetate. The

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CA 02418656 2003-02-06 extract was washed with brine and dried. The solvent was evaporated under reduced pressure and the residue was purified by silica gel chromatography to give l-benzyloxycarbonyl-4-(1cyclohexyl-lH-tetrazol-5-yl)piperazine (390 mg) as a white powder.

(2) The above-mentioned compound (388 mg) was dissolved in ethanol (10 mL) and ethyl acetate (10 mL). The mixture was stirred in the presence of 10% palladium/carbon (140 mg) under a hydrogen atomosphere (1 atm) for 2 hr. The reaction mixture was filtrated and the filtrate was concentrated under reduced pressure to give 1-(l-cyclohexyl-lH-tetrazol-5-yl)piperazine (248 mg) as a white powder.

(3) Using the above-mentioned compound (248 mg) and the title compound (290 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(l-cyclohexyl-lH-tetrazol-5-yl)-l-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (502 mg) was obtained as a white powder.

(4) Using the above-mentioned compound (502 mg), and in the same manner as in Example 133 (2), the title compound (302 mg) was obtained as a white powder.

¹ H-NMR(500MHz,DMSO-dg)61.20-1.34(lH,m), 1.40-1.50(2H,m), 1.641.88(5H,m), 1.97-2.03(2H,m), 2.12-2.32(lH,m), 2.90-4.05(16H,m), 4.25(lH,m), 4.48-4.75(3H,m), 9.10(lH,brs), 10.67(lH,brs).

Example 230

Synthesis of 3-{(2S,4S)-4-[4-(2-benzimidazolyl)-l-piperazinyl]2-pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) Using 2-chlorobenzimidazole (0.500 g) and piperazine (8.47 g), and in the same manner as in Example 189 (1), 1-(2benzimidazolyl)piperazine (0.086 g) was obtained as a white powder.

(2) Using the above-mentioned compound (86 mg) of Reference Example 12 and the title compound (128 mg), and in the same

195

CA 02418656 2003-02-06 manner as in Example 70 (1), 3-{(2S,4S)-4-[4-(2benzimidazolyl)-1-piperazinyl]-1-tert-butoxycarbony1-2pyrrolidinylcarbonyl}-l,3-thiazolidine (203 mg) was obtained as a white solid.

(3) Using the above-mentioned compound (203 mg), and in the same manner as in Example 186 (2), the title compound (94 mg) was obtained as a white powder.

¹H-NMR(DMSO-de)01.72-2.16( lH,m), 2.65-4.30( 16H,m), 4.404.80(3H,m), 7.18-7.33(2H,m), 7.36-7.51(2H,m), 8.95(lH,brs), 9.70(lH,brs), 10.50(1H,brs), 13.71(2H,brs).

Example 231

Synthesis of 3-{(2S,4S)-4-[4-(5-cyano-2-benzimidazolyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine tr ihydrochloride (1) 4-Amino-3-nitrobenzonitrile (25 g) was dissolved in methanol (200 mL) and tetrahydrofuran (200 mL). The mixture was stirred in the presence of 10% palladium/carbon (3.0 g) under a hydrogen atomosphere (1 atm) for 20 hr. The reaction mixture was filtrated and the filtrate was concentrated under reduced pressure to give 3,4-diaminobenzonitrile (20 g) as a charcoal brown solid.

(2) The above-mentioned compound (2.60 g) was dissolved in DMF (20 mL) and pyridine (2 mL), and a solution of triphosgene (2.12 g) in tetrahydrofuran (20 mL) was added dropwise under ice-cooling. The mixture was stirred at room temperature for 18 hr. Dilute hydrochloric acid was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The extract was washed with brine and dried. The solvent was evaporated under reduced pressure and ethyl acetate was added to the residue. The precipitated solid was collected by filtration to give 2-hydroxybenzimidazole-5-carbonitrile (896 mg) as a purple solid.

(3) The above-mentioned compound (894 mg) was dissolved in

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CA 02418656 2003-02-06 phosphorus oxychloride (12 mL), and the solution was refluxed for 3 hr. The reaction mixture was added to ice, and extracted with ethyl acetate. The extract was washed with brine, dried and concentrated under reduced pressure. The residue was purified by silica gel chromatography to give 2chlorobenzimidazole-5-carbonitrile (322 mg) as a white powder.

(4) The product (345 mg) of Example 199 (3) was dissolved in Nmethyl-2-pyrrolidone (6 mL), and the above-mentioned compound (182 mg) and Ν,Ν-diisopropylethylamine (180 μΣ) were added thereto. The mixture was stirred at 100°C for 17 hr. The reaction mixture was added to water, and the mixture was extracted with ethyl acetate. The extract was washed with brine, dried and concentrated under reduced pressure. The residue was purified by HPLC to give 3-{(2S,4S)-lbenzyloxycarbonyl-4-[4-(5-cyano-2-benzimidazolyl)-1piperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine (250 mg) as a white solid.

(5) Using the above-mentioned compound (200 mg), and in the same manner as in Example 199 (5), the title compound (50 mg) was obtained as a white powder.

¹ H-NMR(500MHz,DMSO-de)61.89-2.09(lH,m), 2.78-4.20(16H,m), 4.474.82(3H,m), 7.51(lH,d,J=8.2Hz), 7.61(lH,d,J=8.2Hz), 7.81(lH,s), 8.97(lH,brs), 10.28(lH,brs).

Example 232

Synthesis of 3-{(2S,4S)-4-[4-(5-trifluoromethyl-2benzimidazolyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-l,3thiazolidine trihydrobromide (1) Using 4-trifluoromethyl-2-nitroaniline (25.0 g), and in the same manner as in Example 231 (1), 4-trifluoromethy1-1,2phenylenediamine (21.3 g) was obtained as a white solid.

(2) Using the above-mentioned compound (10.2 g) and in the same manner as in Example 231 (2), the reaction mixture was added to water. The precipitated solid was collected by filtration to

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CA 02418656 2003-02-06 give 5-trifluoromethyl-2-hydroxybenzimidazole (3.28 g) as a white solid.

(3) Using the above-mentioned compound (3.27 g), and in the same manner as in Example 231 (3), 2-chloro-5trifluoromethylbenzimidazole (2.48 g) was obtained as a white powder.

(4) Using the above-mentioned compound (226 mg) and the product (345 mg) of Example 199 (3), and in the same manner as in Example 231 (4), 3-{(2S,4S)-l-benzyloxycarbony1-4-(4-(5- trifluoromethyl-2-benzimidazolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (192 mg) was obtained as a white solid.

(5) 30% Hydrogen bromide-acetic acid solution (10 mL) was added to the above-mentioned compound (190 mg), and the mixture was stirred at room temperature for 6 hr. Diethyl ether was added to the reaction mixture, and the precipitated solid was collected by filtration and washed with ethanol. The title compound (101 mg) was obtained as a white powder.

¹ H-NMR(500MHz,DMSO-d₆)61.761.96(lH,m), 2.75-4.80(16H,m), 4.464.74(3H,m), 7.60(2H,s), 7.67(lH,s), 8.96(lH,brs), 9.59(lH,brs), 13.02(lH,brs).

Example 233

Synthesis of 3-{(2S,4S)-4-[4-(5-fluoro-2-benzimidazolyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-1,3-thiazolidine tr ihydrobromide (1) Using 4-fluoro-2-nitroaniline (25.0 g), and in the same manner as in Example 231 (1), 4-fluoro-l,2-phenylenediamine (20.1 g) was obtained as a charcoal brown solid.

(2) Using the above-mentioned compound (10.0 g), and in the same manner as in Example 231 (2), the reaction mixture was added to water. The precipitated solid was collected by filtration to give 5-fluoro-2-hydroxybenzimidazole (2.14 g) as a brown solid.

198

CA 02418656 2003-02-06 (3) Using the above-mentioned compound (2.13 g), and in the same manner as in Example 231 (3), 2-chloro-5fluorobenzimidazole (1.44 g) was obtained as a brown solid.

(4) Using the above-mentioned compound (174 mg) and the product (345 mg) of Example 199 (3), and in the same manner as in Example 231 (4), 3-{(2S,4S)-l-benzyloxycarbonyl-4-[4-(5-fluoro2-benzimidazolyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-l,3thiazolidine (66 mg) was obtained as a white solid.

(5) Using the above-mentioned compound (66 mg), and in the same manner as in Example 232 (5), the title compound (20 mg) was obtained as a white powder.

¹ H-NMR(500MHz,DMSO-de)61.77-1.97(lH,m), 2.88-4.20(16H,m), 4.464.76(3H,m), 7.12-7.16(lH,m), 7.32(lH,dd,J=8.5,2.2Hz),

7.44(lH,dd,J=8.7,4.5Hz), 8.97(1H,brs), 9.61(lH,brs),

13.16(lH,brs).

Example 234

Synthesis of 3-{(2S,4S)-4-[4-(5-chloro-2-benzimidazolyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrobromide (1) 2-Chlorobenzimidazole (1.05 g) was dissolved in DMF (10 mL), and N-chlorosuccinimide (1.01 g) was added thereto. The mixture was stirred at 60°C for 30 min and the reaction mixture was added to water. The precipitated solid was collected by filtration to give 2,5-dichlorobenzimidazole (0.480 g) as a white solid.

(2) Using the above-mentioned compound (191 mg) and the product (345 mg) of Example 199 (3), and in the same manner as in Example 231 (4), 3-{(2S,4S)-l-benzyloxycarbonyl-4-[4-(5-chloro2-benzimidazolyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-l,3thiazolidine (122 mg) was obtained as a pale-yellow solid.

(3) Using the above-mentioned compound (110 mg), and in the same manner as in Example 232 (5), the title compound (56 mg) was obtained as a white powder.

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27103-383 ¹ H-NMR(500MHz,DMSO-de)δ1.76-1.96(lH,m), 2.70-4.87(16H,m), 4.464.74(3H,m), 7.32(1H,dd,J=8.5,1.7Hz), 7.44(1H,d,J=8.5Hz),

7.48(lH,d,J=1.7Hz), 8.96(lH,brs), 9.59(lH,brs), 13.15(lH,brs).

Example 235

Synthesis of 3-{(2S,4S)-4-[4-(5-nitro-2-benzimidazolyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-!,3-thiazolidine trihydrochloride (1) Using 4-nitro-l,2-phenylenediamine (9.12 g), and in the same manner as in Example 231 (2), the reaction mixture was io added to water. The precipitated solid was collected by filtration to give S-nitro-2-hydroxybenzimidazole (5.69 g) as an yellow solid.

(2) Using the above-mentioned compound (5.69 g), and in the same manner as in Example 231 (3), 2-chloro-5- nitrobenzimidazole (2.41 g) was obtained as a yellow solid.

(3) Using the above-mentioned compound (1.00 g) and piperâzine (4.70 g), and by a reaction at 100°C in the same manner as in Example 196 (1), 1-(5-nitro-2-benzimidazolyl)piperazine (0.16 g) was obtained as an orange solid.

(4) Using the above-mentioned compound (160 mg) and the title compound (162 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-((2S,4S)-l-tert-butoxycarbonyl4-(4-(5-nitro-2-benzimidazolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (172 mg) was obtained as a yellow solid.

(5) Using the above-mentioned compound (172 mg), and in the same manner as in Example 133 (2), the title compound (138 mg) was obtained as a yellow powder.

¹ H-NMR(500MHz,DMSO-d₆ )02.08-2.24(lH,m), 2.78-4.03(16H,m), 4.4830 4.75(3H,m), 7.52(2H,d,J=8.6Hz), 8 .ll(2H,dd,J=8.6,2.0Hz),

8.12(1H,d,J=2.0Hz), 9.06(lH,brs), 10.59(lH,brs).

Example 236

Synthesis of 3-{(2S,4S)-4-(4-(l-methyl-2-benzimidazolyl)-l200

CA 02418656 2003-02-06 pipera zinyl]-2-pyrrolidinylcarbonyl}-1*3-thiazolidine trihydrochloride (1) Sodium hydride (contained by 60%, 0.288 g) was suspended in DMF (10 mL), and 2-chlorobenzimidazole (1 g) was added thereto.

The mixture was stirred at room temperature for 30 min and methyl iodide (Ô.61 mL) was added thereto. After stirring at room temperature for 1 hr, water was added to the reaction mixture. The mixture was extracted with ethyl acetate, and the extract was washed with brine and concentrated under reduced io pressure to give 2-chloro-l-methylbenzimidazole (0.928 g) as a white powder.

(2) Using the above-mentioned compound (0.928 g) and piperazine (9.60 g), and in the same manner as in Example 189 (1), 1-(1methyl-2-benzimidazolyl)piperazine (1.18 g) was obtained as a is pale-yellow solid.

(3) Using the above-mentioned compound (476 mg) and the title compound (601 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-1-tert-butoxycarbony14-(4-(l-methyl-2-benzimidazolyl)-l-piperazinyl]-2- pyrrolidinylcarbonyl}-l,3-thiazolidine (947 mg) was obtained as a white solid.

(4) Using the above-mentioned compound (857 mg), and in the same manner as in Example 186 (2), the title compound (532 mg) was obtained as a white powder.

¹H-NMR(DMSO-de)62.19-2.38( lH,m), 2.89-3.20(3H,m), 3.304.30(13H,m), 3.79(3H,s), 4.42-4.85(3H,m), 7.34-7.47(2H,m),

7.53-7.63(lH,m), 7.64-7.76(lH,m), 9.15(lH,brs), 11.08(lH,brs). Example 237

Synthesis of 3-{(2S,4S)-4-(4-(5-trifluoromethyl-l-methyl-230 benzimidazolyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-1,3thiazolidine trihydrochloride (1) 4-Fluoro-3-nitrobenzotrifluoride (25 g) was dissolved in ethanol (50 mL), and 30% methylamine-ethanol solution (97.9 g)

201

CA 02418656 2003-02-06 was gradually added dropwise under ice-cooling, and the mixture was stirred at room temperature for 40 min. The reaction mixture was added to water, and the precipitated solid was collected by filtration to give 4-methylamino-3nitrobenzotrifluoride (25.5 g) as yellow crystals.

(2) Using the above-mentioned compound (25.3 g), and in the same manner as in Example 231 (1), 4-trifluoromethy1-Nl-methyl-

1,2-phenylenediamine (21.9 g) was obtained as a pale-yellow solid.

(3) The above-mentioned compound (21.9 g) was subjected to the reaction in the same manner as in Example 231 (2), and water was added to the reaction mixture. The precipitated solid was collected by filtration to give 5-trifluoromethyl-2-hydroxy-lmethylbenzimidazole (23.8 g) as a white solid.

(4) Using the above-mentioned compound (10.1 g), and in the same manner as in Example 231 (3), 2-chloro-5-trifluoromethyl1-methylbenzimidazole (10.5 g) was obtained as a white solid.

(5) Using the above-mentioned compound (5.07 g) and piperazine (18.6 g), and by a reaction at 100°C in the same manner as in Example 196 (1), 1-(5-trifluoromethyl-l-methyl-2benzimidazolyl)piperazine (4.87 g) was obtained as a white solid.

(6) Using the above-mentioned compound (485 mg) and the title compound (518 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(5-trifluoromethyl-l-methyl-2-benzimidazolyl)-1piperazinyl]-2-pyrrolidinylcarbonyl)-l,3-thiazolidine (978 mg) was obtained as a white powder.

(7) Using the above-mentioned compound (978 mg), and in the same manner as in Example 133 (2), the title compound (483 mg) was obtained as a white powder.

¹ H-NMR(500MHz,DMSO-d₆)Ô2.23-2.43(lH,m), 2.97-4.15(16H,m),

3.76(3H,s), 4.49-4.77(3H,m), 7.61(1H,d,J=8.4Hz),

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7.74(lH,d,J=8.4Hz), 7.80(lH,s), 9.14(lH,brs), 10.94(lH,brs).

Example 238

Synthesis of 3-{(2S,4S)-4-[4-(5-fluoro-l-methy1-2benzimidazolyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-l,35 thiazolidine trihydrochloride (1) Using 2,5-difluoronitrobenzene (25.4 g), and in the same manner as in Example 237 (1), 5-fluoro-2(methylamino)nitrobenzene (27.2 g) was obtained as an orange solid.

io (2) Using the above-mentioned compound (27.2 g), and in the same manner as in Example 231 (1), 4-fluoro-Nl-methyl-l,2phenylenediamine (20.9 g) was obtained as an orange solid.

(3) Using the above-mentioned compound (18.1 g), and in the same manner as in Example 231 (2), 5-fluoro-2-hydroxy-l- methylbenzimidazole (0.682 g) was obtained as a pale-yellow solid.

(4) Using the above-mentioned compound (675 mg), and in the same manner as in Example 231 (3), 2-chloro-5-fluoro-lmethylbenzimidazole (647 mg) was obtained as a white solid.

(5) Using the above-mentioned compound (0.633 g) and piperazine (3.2 g), and by a reaction at 100°C in the same manner as in Example 196 (1), 1-(5-fluoro-l-methy1-2benzimidazolyl)piperazine (0.77 g) was obtained as a paleyellow solid.

(6) Using the above-mentioned compound (0.76 g) and the title compound (0.80 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-[4-(5-fluoro-l-methyl-2-benzimidazolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (1.40 g) was obtained as a white powder.

(7) Using the above-mentioned compound (1.40 g), and in the same manner as in Example 133 (2), the title compound (0.676 g) was obtained as a white powder.

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27103-383 ¹ H-NMR(500MHz,DMSO-d₆)62.2 0-2.40(1H,m), 2.8 5-4.14(16H,m),

3.76(3H,s), 4.49-4.76(3H,m), 7.23(lH,m),

7.38(lH,dd,J=8.6,2.1Hz), 7.65(lH,dd,J=8.7,4.3Hz), 9.13(lH,brs), 11.03(lH,brs).

Example 239

Synthesis of 3-{(2S,4S)-4-(4-(5-cyano-2-benzoxazolyl)-1pipera ziny1]-2-pyrrolidinylcarbonyl}-1,3-thiazolidine dihydrochloride (1) 3-Amino-4-cyanophenol (6.71 g) was dissolved in pyridine io (100 mL), and potassium ethylxanthate (8.82 g) was added thereto. The mixture was refluxed for 2 hr. After allowing to cool, iced water (200 mL) was added to the reaction mixture, and cone, hydrochloric acid (40 mL) was added thereto. The precipitated solid was collected by filtration to give 5-cyano15 2-mercaptobenzoxazole (5.62 g) as a gray powder.

(2) The above-mentioned compound (5 g) and two drops of DMF were added to thionyl chloride (20 mL), and the mixture was refluxed for 1 hr. The reaction mixture was concentrated under reduced pressure to give 2-chloro-5-cyanobenzoxazole (5.06 g) .' (3) Piperazine (4.29 g) was dissolved in DMF (40 mL), and the above-mentioned compound (2.96 g) was added thereto. The mixture was stirred at room temperature for 3 hr. The reaction mixture was added to water, and the mixture was extracted with chloroform. 1 mol/L Hydrochloric acid was added to the extract, and the aqueous layer was separated. Aqueous sodium hydroxide solution was added thereto, and the mixture was extracted with chloroform. The extract was concentrated under reduced pressure to give 1-(5-cyano-2-benzoxazolyl)piperazine (0.933 g) as a pale-yellow powder.

(4) Using the above-mentioned compound (502 mg) and the title compound (601 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-I-tert-butoxycarbonyl4-(4-( 5-cyano-2-benzoxazolyl) -1-piperazinyl]-2204

CA 02418656 2003-02-06 pyrrolidinylcarbonyl}-l,3-thiazolidine (428 mg) was obtained as a white powder.

(5) The above-mentioned compound (424 mg) was dissolved in chloroform (10 mL), and 5 mol/L hydrochloric acid-ethyl acetate (5 mL) was added thereto. The mixture was stirred at room temperature for 18 hr and the reaction mixture was concentrated under reduced pressure. The residue was washed with ethanol to give the title compound (302 mg) as a white powder.

^XH-NMR( DMSO-de )61.94-2.26 (lH,m), 2.80-3.00 ( lH,m), 3.004.30(15H,m), 4.45-4.78(3H,m), 7.56(lH,dd,J=8.4,1.8Hz), 7.66(lH,d,J=8.4Hz), 7.83(lH,d,J=1.5Hz), 9.05(lH,brs), 10.43(1H,brs).

Example 240

Synthesis of 3-((2S,4S)-4-(4-(5-cyano-2-benzothiazolyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) 2-Bromo-5-nitroaniline (10 g) was dissolved in N-methyl-2pyrrolidone (50 mL), and potassium ethylxanthate (14.8 g) was added thereto. The mixture was stirred at 140°C with heating. The reaction mixture was concentrated under reduced pressure, and water (300 mL) and cone, hydrochloric acid (10 mL) were added to the residue. The precipitated solid was collected by filtration, which was then dissolved in 1 mol/L aqueous sodium hydroxide solution (80 mL). After washing with chloroform, 1 mol/L hydrochloric acid was added thereto, and the precipitated solid was collected by filtration to give 2-mercapto-5nitrobenzothiazole (8.43 g) as an orange powder.

(2) Sodium hydride (contained by 60%, 1.05 g) was suspended in DMF (50 mL) and the above-mentioned compound (8.43 g) was added thereto under ice-cooling. After the mixture was stirred for 30 min, methyl iodide (2.72 mL) was added thereto under icecooling. The mixture was stirred at room temperature for 2 hr. The reaction mixture was added to water, and the mixture was

205

CA 02418656 2003-02-06 extracted with ethyl acetate (insoluble material was removed by filtration). The extract was washed successively with water and brine and concentrated under reduced pressure to give 2methylthio-5-nitrobenzothiazole (4.82 g) as a pale-brown powder.

(3) The above-mentioned compound (3.33 g) was dissolved in ethanol (70 mL), and chloride dihydrate (II) (14.0 g) was added thereto. The mixture was refluxed for 1 hr. The reaction mixture was concentrated under reduced pressure, and saturated aqueous sodium hydrogencarbonate solution was added to the residue. The mixture was extracted with chloroform (insoluble material was filtered off). The extract was washed with brine and concentrated under reduced pressure to give 5-amino-2methylthiobenzothiazole (2.54 g) as a red-orange solid.

(4) The above-mentioned compound (2.54 g) was suspended in water (40 mL) and cone, hydrochloric acid (3.75 mL) was added thereto under ice-cooling. Thereto was added dropwise a solution of sodium nitrite (1.00 g) in water (10 mL) under icecooling. After stirring the reaction mixture for 20 min, 5% aqueous potassium carbonate solution (20 mL) was added dropwise under ice-cooling. This reaction mixture was added dropwise under ice-cooling to a solution of copper cyanide (2.35 g) and potassium cyanide (3.44 g) in water (40 mL) prepared separately. After stirring under ice-cooling for 1 hr, the mixture was stirred at 50°C for 10 min with heating. The reaction mixture was added to water (50 mL), and the mixture was extracted with ethyl acetate (insoluble material was filtered off). The extract was washed with brine, and concentrated under reduced pressure to give 5-cyano-2-methylthiobenzothiazole (1.96 g).

(5) Using the above-mentioned compound (0.645 g) and piperazine (8.08 g), and in the same manner as in Example 189 (1), 1-(5cyano-2-benzothiazolyl)piperazine (0.601 g) was obtained as a brown solid.

(6) Using the above-mentioned compound (601 mg) and the title

206

CA 02418656 2003-02-06 compound (601 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(5-cyano-2-benzothiazolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (649 mg) was obtained as a pale-orange powder.

(7) Using the above-mentioned compound (649 mg), and in the same manner as in Example 239 (5), the title compound (500 mg) was obtained as a white powder.

¹H-NMR(DMSO-d₆)02.04-2.28( lH,m), 2.82-3.00( lH,m), 3.004.30(15H,m), 4.43-4.80(3H,m), 7.53(lH,dd,J=8.2,1.5Hz), 7.93(lH,d,J=1.5Hz), 8.07(lH,d,J=8.2Hz), 9.08(lH,brs), 10.51(lH,brs).

Example 241

Synthesis of 3-{(2S,4S)-4-[4-(6-cyano-2-benzothiazolyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) Sodium hydride (containing by 60%, 6.15 g) was suspended in DMF (120 mL) and 2-mercapto-6-nitrobenzothiazole (20 g) was added thereto under ice-cooling. After completion of bubbling, methyl iodide (26.4 mL) was added thereto. The mixture was stirred at room temperature for 18 hr. Water (800 mL) was added to the reaction mixture, and the precipitated solid was collected by filtration to give 2-methylthio-6nitrobenzothiazole (21.2 g) as a pale-yellow powder.

(2) Using the above-mentioned compound (10 g) and tin(II) chloride (41.9 g), and in the same manner as in Example 240 (3),

6-amino-2-methylthiobenzothiazole (7.75 g) was obtained as a white solid.

(3) Using the above-mentioned compound (2.59 g), sodium nitrite (1.00 g), copper cyanide (2.35 g) and potassium cyanide (3.44 g), and in the same manner as in Example 240 (4), 6-cyano-2mercaptobenzothiazole (2.22 g) was obtained as a brown solid.

(4) Using the above-mentioned compound (2.04 g) and piperazine

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CA 02418656 2003-05-01

27103-383 (8.82 g), and in the same manner as in Example 189 (I), 1-(6cyano-2-benzothiazolyl)piperazine (2.02 mg) was obtained as a brown-reddish solid.

(5) Using the above-mentioned compound (538 mg) and the title compound (601 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(23,4S)-l-tert-butoxycarbonyl4-[4-(6-cyano-2-benzothiazolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (526 mg) was obtained as an orange powder.

io (6) Using the above-mentioned compound (526 mg), and in the same manner as in Example 239 (5), the title compound (394 mg) was obtained as a white powder.

¹H-NMR(DMSO-d6 )02.00-2.30 ( lH,m), 2.80-3.00 ( lH,m), 3.004.30(15H,m), 4.42-4.80(3H,m), 7.59(lH,d,J=8.4Hz),

7.72(lH,dd,J=8.1,1.5Hz), 8.39(lH,d,J=1.5Hz), 9.06(lH,brs),

10.58(lH,brs).

Example 242

Synthesis of 3-((2S,4S)-4-[4-(6-trifluoromethyl-2benzothiazolyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-l,320 thiazolidine dihydrochloride (1) 4-Amino-3-bromobenzotrifluoride (2.40 g) was dissolved in N-methy1-2-pyrrolidone (10 mL), and potassium ethylxanthate (3.52 g) was added thereto. The mixture was stirred at 160°C for 3 hr with heating. Water (300 mL) was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The extract was washed successively with water and brine, and concentrated under reduced pressure to give 6trifluoromethyl-2-mercaptobenzothiazole (607 mg) as an orange powder.

(2) Using the above-mentioned compound (607 mg), sodium hydride (containing by 60%, 155 mg) and methyl iodide (241 mL), and in the same manner as in Example 236 (1), 6-trifluoromethyl-2methylthiobenzothiazole (665 mg) was obtained as a brown solid.

208

CA 02418656 2003-02-06 (3) Using the above-mentioned compound (0.665 g) and piperazine (11.5 g), and in the same manner as in Example 189 (1), 1-(6trifluoromethylbenzothiazolyl)piperazine (0.56 g) was obtained as a white powder.

(4) Using the above-mentioned compound (560 mg) and the title compound (532 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-[4-(6-trifluoromethyl-2-benzothiazolyl)-l-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (477 mg) was obtained as io a white powder.

(5) Using the above-mentioned compound (477 mg), and in the same manner as in Example 186 (2), the title compound (403 mg) was obtained as a white powder.

^XH-NMR( DMSO-de )62.05-2.33 (lH,m), 2.79-3.00( lH,m), 3.0015 3.29(2H,m), 3.29-4.30(13H,m), 4.45-4.80(3H,m), 7.58-7.70(2H,m), 8.34(lH,s), 9.10(lH,brs), 10.72(lH,brs).

Example 243

Synthesis of 3-{(2S,4S)-4-[4-(6-methoxy-2-benzothiazolyl)-lpipera ziny1]-2-pyrrolidinylcarbonyl}-1,3-thia zolidine trihydrochloride (1) Using 2-chloro-6-methoxybenzothiazole (1 g) and piperazine (8.63 g), and in the same manner as in Example 189 (1), 1-(6methoxybenzothiazolyl)piperazine (1.22 g) was obtained as a pale-brown powder.

(5) Using the above-mentioned compound (549 mg) and the title compound (601 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-[4-(6-methoxy-2-benzothiazolyl)-l-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (420 mg) was obtained as 30 a white powder.

(6) Using the above-mentioned compound (420 mg), and in the same manner as in Example 186 (2), the title compound (393 mg) was obtained as a white powder.

209

CA 02418656 2003-02-06 ¹H-NMR( DMSO-d₆ )02.22-2.41 ( lH,m), 2.90-3.20(3H,m), 3.254.20(13H,m), 3.77(3H,s), 4.42-4.81(3H,m),

6.96(lH,dd,J=8.9,2.6Hz), 7.47(lH,d,J=8.8Hz), 7.50(lH,d,J=2.6Hz). Example 244

Synthesis of 3-{(2S,4S)-4-[4-(6-isopropoxy-2-benzothiazolyl)-lpipera ziny1]-2-pyrrolidinylcarbonyl}-1,3-thia zolidine trihydrochloride (1) 4-Isopropoxyaniline (24.2 g) was dissolved in acetic acid (300 mL), and a mixture of bromine (8.25 mL) and acetic acid (80 mL) was added dropwise. After stirring at room temperature for 2 hr, the reaction mixture was concentrated under reduced pressure. Water was added to residue, and the mixture was extracted with diethylether. The extract was washed successively with aqueous sodium thiosulfate solution and brine, and concentrated under reduced pressure. The residue was purified by silica gel chromatography to give 2-bromo-4isopropoxyaniline (10.2 g) as a black brown oil.

(2) The above-mentioned compound (10.2 g) was dissolved in Nmethyl-2-pyrrolidone (50 mL), and potassium ethylxanthate (14.2 g) was added thereto. The mixture was stirred at 140°C for 6 hr with heating. The reaction mixture was concentrated under reduced pressure, and 1 mol/L aqueous sodium hydroxide solution (50 mL) was added to the residue. The mixture was washed with chloroform, and cone, hydrochloric acid (30 mL) was added thereto. The mixture was extracted with chloroform. The extract was concentrated under reduced pressure to give 6isopropoxy-2-mercaptobenzothiazole (12.6 g) as a black brown oil.

(3) The above-mentioned compound (11.6 g) and 2 drops of DMF were added to thionyl chloride (30 mL), and the mixture was stirred at room temperature for 18 hr. The reaction mixture was concentrated under reduced pressure, and saturated aqueous sodium hydrogencarbonate solution was added to the residue.

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The mixture was extracted with chloroform. The extract was concentrated under reduced pressure and the residue was purified by silica gel chromatography to give 2-chloro-6isopropoxybenzothiazole (9.37 g) as a black oil.

(4) Using the above-mentioned compound (9.37 g) and piperazine (35.4 g), and in the same manner as in Example 189 (1), 1-(6isopropoxy-2-benzothiazolyl)piperazine (2.8 g) was obtained as a black powder.

(5) Using the above-mentioned compound (1.25 g) and the title compound (0.901 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(6-isopropoxy-2-benzothiazolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (0.993 g) was obtained as a pale-brown powder.

(6) Using the above-mentioned compound (933 mg), and in the same manner as in Example 186 (2), the title compound (749 mg) was obtained as a white powder.

¹H-NMR( DMSO-de )ôl .26 ( 6H, d, J=6.0Hz ), 2.20-2.40 ( lH,m), 2.903.20(3H,m), 3.28-4.12(14H,m), 4.39-4.80(3H,m),

6.92(lH,dd,J=8.8,2.6Hz), 7.44(lH,d,J=8.8Hz), 7.48(lH,d,J=2.6Hz), 9.18(lH,brs), 10.90(1H,brs).

Example 245

Synthesis of 3-{(2S,4S)-4-[4-(5-nitro-2-benzothiazolyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) Using 2-methylthio-5-nitrobenzothiazole (product of Example 240 (2), 1.5 g] and piperazine (11.4 g), and in the same manner as in Example 189 (1), l-(5-nitro-2-benzothiazolyl)pipèrazine (1.55 g) was obtained as a yellow powder.

(2) Using the above-mentioned compound (581 mg) and the title compound (601 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(5-nitro-2-benzothiazolyl)-1-piperazinyl]-2211

CA 02418656 2003-02-06 pyrrolidinylcarbonyl}-l,3-thiazolidine (507 mg) was obtained as a yellow powder.

(3) Using the above-mentioned compound (507 mg), and in the same manner as in Example 186 (2), the title compound (243 mg) was obtained as a yellow powder.

^xH-NMR(DMSO-d6)02.00-2.22( lH,m), 2.79-3.00( lH,m), 3.004.30(15H,m), 4.42-4.80(3H,m), 7.98(lH,dd,J=8.7,2.2Hz), 8.13(lH,d,J=8.7Hz), 8.21(lH,d,J=2.2Hz), 9.05(lH,brs), 10.40(1H,brs).

Example 246

Synthesis of 3-((2S,4S)-4-(4-(6-nitro-2-benzothiazolyl)-1piperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) Cone, sulfuric acid (50 mL) was added to 2chlorobenzothiazole (10 g) under ice-cooling, and cone, nitric acid (5 mL) was added dropwise under ice-cooling. The mixture was stirred under ice-cooling for 1 hr, and iced water (600 mL) was added to the reaction mixture. The precipitated solid was collected by filtration, which was then recrystallized from acetone to give 2-chloro-6-nitrobenzothiazole (6.36 g) as a pale-yellow powder.

(2) Using the above-mentioned compound (6.36 g) and piperazine (25.8 g), and in the same manner as in Example 189 (1), 1-(6nitro-2-benzothiazolyl)piperazine (1.84 g) was obtained as a yellow powder.

(3) Using the above-mentioned compound (581 mg) and the title compound (601 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-((2S,4S)-l-tert-butoxycarbonyl4-(4-(6-nitro-2-benzothiazolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (664 mg) was obtained as a yellow powder.

(4) Using the above-mentioned compound (588 mg), and in the same manner as in Example 186 (2), the title compound (495 mg)

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CA 02418656 2003-02-06 was obtained as a yellow powder.

¹H-NMR(DMSO-d₆)ô2.00-2.26(lH,m), 2.83-3.00(lH,m), 3.014.30(15H,m), 4.43-4.80(3H,m), 7.60(lH,d,J=9.0Hz), 8.19(lH,dd,J=9.0,2.4Hz), 8.89(lH,d,J=2.4Hz), 9.16(lH,brs), 10.45(1H,brs).

Example 247

Synthesis of 3-{(2S,4S)-4-[4-(6-fluoro-2-benzothiazolyl)-lpipera z iny1]-2-pyrrolidinylcarbonyl}-1,3-thia zolidine dihydrochloride (1) Using 2-bromo-4-fluoroaniline (25 g) and potassium ethylxanthate (42.2 g), and in the same manner as in Example 244 (2), 6-fluoro-2-mercaptobenzothiazole (1.01 g) was obtained as a white powder.

(2) Using the above-mentioned compound (1.01 g), sodium hydride (containing by 60%, 0.24 g), and methyl iodide (373 mL), and in the same manner as in Example 236 (1), 6-fluoro-2methylthiobenzothiazole (0.928 g) was obtained as a pale-yellow solid.

(3) Using the above-mentioned compound (0.928 g) and piperazine (8.02 g), and in the same manner as in Example 189 (1), 1-(6fluoro-2-benzothiazolyl)piperazine (0.627 g) was obtained as a pale-yellow oil.

(4) Using the above-mentioned compound (627 mg) and the title compound (601 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-&lt;(2S,4S)-1-tert-butoxycarbony14-(4-(6-fluoro-2-benzothiazolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (535 mg) was obtained as a white powder.

(5) Using the above-mentioned compound (535 mg), and in the same manner as in Example 186 (2), the title compound (441 mg) was obtained as a white powder.

¹H-NMR( DMSO-de )02.05-2.38 ( lH,m), 2.82-4.30 ( 16H,m), 4.424.80(3H,m), 7.17(lH,td,J=9.0,2.7Hz), 7.52(1H,dd,J=9.0,4.8Hz),

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7.79(lH,dd,J=8.7,2.7Hz), 9.09(lH,brs), 10.72(lH,brs).

Example 248

Synthesis of 3-{(2S,4S)-4-(4-(5-chloro-2-benzothiazolyl)-1piperazinyl]-2-pyrrolidinylcarbonyl}-1,3-thiazolidine trihydrochloride (1) Using 5-chloro-2-mercaptobenzothiazole (25 g), sodium hydride (containing by 60%, 5.45 g) and methyl iodide (8.49 mL), and in the same manner as in Example 241 (1), 5-chloro-2methylthiobenzothiazole (26.7 g) was obtained as a white powder.

(2) Using the above-mentioned compound (10.8 g) and piperazine (43.1 g), and in the same manner as in Example 189 (1), 1-(6chloro-2-benzothiazolyl)piperazine (10.9 g) was obtained as a white powder.

(3) Using the above-mentioned compound (558 mg) and the title compound (601 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(5-chloro-2-benzothiazolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (834 mg) was obtained as a white powder.

(4) Using the above-mentioned compound (834 mg), and in the same manner as in Example 186 (2), the title compound (735 mg) was obtained as a white powder.

^xH-NMR(DMSO-d₆)02.20-2.40( lH,m), 2.89-3.20(3H,m), 3.233.60(4H,m), 3.60-4,85(12H,m), 7.18(lH,dd,J=8.4,2.1Hz), 7.56(lH,d,J=2.1Hz), 7.88(lH,d,J=8.4Hz), 9.15(lH,brs), 10.86(lH,brs).

Example 249

Synthesis of 3-{(2S,4S)-4-[4-(6-chloro-2-benzothiazolyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine tr ihydroc hloride (1) Using 2,6-dichlorobenzothiazole (1 g) and piperazine (8.44 g), and in the same manner as in Example 189 (1), l-(6-chloro2-benzothiazolyl)piperazine (1.24 g) was obtained as a pale214

CA 02418656 2003-02-06 brown solid .

(2) Using the above-mentioned compound (558 mg) and the title compound (601 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(6-chloro-2-benzothiazolyl)-1-piperaziny1]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (740 mg) was obtained as a white powder.

(3) Using the above-mentioned compound (647 mg), and in the same manner as in Example 186 (2), the title compound (603 mg) was obtained as a white powder.

¹H-NMR(DMSO-d₆)ô2.22-2.42( lH,m), 2.90-3.21(3H,m), 3.304.20(13H,m), 4.43-4.81(3H,m), 7.36(lH,dd,J=8.5,2.2Hz), 7.52(lH,d,J=8.5Hz), 8.01(lH,d,J=2.2Hz), 9.25(lH,brs), 11.10(lH,brs).

Example 250

Synthesis of 3-{(2S,4S)-4-(4-(l-methyl-lH-indazol-3-yl)-1piperazinyl]-2-pyrrolidinylcarbonyl}-1,3-thiazolidine trihydrochloride (1) Using 1-(1-methyl-lH-indazo1-3-yl)piperazine (0.714 g) and the title compound (0.901 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tertbutoxycarbonyl-4-[4-(l-methyl-lH-indazol-3-yl)-l-piperazinyl]2-pyrrolidinylcarbonyl}-l,3-thiazolidine (1.37 g) was obtained as a pale-yellow solid.

¹H-NMR(CDCl₃)01.41(4.5H,s), 1.46(4.5H,s), 1.85-2.00(lH,m),

2.42-2.55(lH,m), 2.62-2.80(4H,m), 2.83-3.22(3H,m),

3.35(lH,t,J=10.1Hz), 3.40-3.52(4H,m), 3.63-4.14(6H,m), 4.394.81(3H,m), 7.01(lH,t,J=7.6Hz), 7.23-7.38(2H,m),

7.67(lH,d,J=8.2Hz).

(2) The above-mentioned compound (1.36 g) was dissolved in ethanol (3.5 mL), and 4.1 mol/L hydrochloric acid-ethanol (3.5 mL) was added thereto. The mixture was stirred at room temperature for 15 hr. The reaction mixture was concentrated

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CA 02418656 2003-02-06 under reduced pressure, and the obtained crystal was recrystallized from ethanol to give the title compound (0.985

g) as a white powder.

¹ H-NMR(DMSO-de )02.30-2.42 ( lH,m), 2.95-3.17 ( 3H,m), 3.24.2(16H,m), 4.46-4.78(3H,m), 7.02-7.08(lH,m), 7.35-7.42(lH,m), 7.51(lH,d,J=8.6Hz), 7.81(lH,d,J=8.2Hz), 9.17(lH,brs),

10.86(1H,brs), 12.40(1H,brs).

Example 251

Synthesis of 3-{(2S,4S)-4-(4-(l-phenyl-lH-indazol-3-yl)-lpipera zinyl]-2-pyrrolidinylcarbony1}-1,3-thia zolidine tr ihydrochloride (1) Using 1-(l-phenyl-lH-indazol-3-yl)piperazine (0.918 g) and the title compound (0.901 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tertbutoxycarbonyl-4-[4-(l-phenyl-lH-indazol-3-yl)-1-piperazinyl]2-pyrrolidinylcarbonyl}-l,3-thiazolidine (1.23 g) was obtained as a white solid.

¹H-NMR(CDC1₃)61.41(4.5H,s), 1.47(4.5H,S), 1.88-2.04(lH,m),

2.45-2.57(lH,m), 2.66-3.18(7H,m), 3.38(lH,t,J=9.9Hz), 3.504.15(7H,m), 4.39-4.80(3H,m), 7.11(1H,t,J=7.5Hz), 7.247.32(lH,m), 7.40(lH,t,J=7.6Hz), 7.49(2H,t,J=7.5Hz), 7.667.78(4H,m).

(2) The above-mentioned compound (1.22 g) was dissolved in ethanol (3 mL), and 4.1 mol/L hydrochloric acid-ethanol (6 mL) was added thereto. The mixture was stirred at room temperature for 14 hr. The precipitated solid was collected by filtration to give the title compound (0.823 g) as a white powder.

¹ H-NMR(DMSO-d₆)02.30-2.46(lH,m), 2.96-3.18(3H,m), 3.34.2(13H,m), 4.46-4.79(3H,m), 7.17-7.25(lH,m),

7.33(1H,t,J=7.4Hz), 7.46-7.58(3H,m), 7.74(2H,d,J=7.6Hz),

7.81(lH,d,J=8.6Hz), 7.98(lH,d,J=8.2Hz), 9.18(lH,brs),

10.94(1H,brs), 12.50(lH,brs).

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CA 02418656 2003-02-06

Example 252

Synthesis of 3-{(2S,4S)-4-[4-(3-benz[d]isoxazolyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine tr ihydroc hloride (1) Hydroxylammonium hydrochloride (15 g) was dissolved in 10% aqueous sodium hydroxide solution (220 mL), and a solution of ethyl salicylate (24 g) in 1,4-dioxane (70 mL) was gradually added, and the mixture was stirred at room temperature for 5 hr. The reaction mixture was concentrated until the amount thereof became almost the half, and acidified with cone, hydrochloric acid. The precipitated solid was collected by filtration, which was then recrystallized from methanol to give salicylhydroxamic acid (12 g) as white crystals.

(2) The above-mentioned compound (12 g) was suspended in tetrahydrofuran (30 mL) and thionyl chloride (13 mL) was added thereto. The mixture was stirred at room temperature for 1 hr. The reaction mixture was concentrated under reduced pressure, and the residue was dissolved in 1,4-dioxane (30 mL). To this solution was gradually added triethylamine (33 mL), and the mixture was stirred at room temperature for 2 hr. The reaction mixture was acidified with cone, hydrochloric acid, extracted with toluene. The extract was washed successively with water and brine, dried and concentrated under reduced pressure. The residue was washed with diisopropyl ether to give 3hydroxybenz[d]isoxazole (3.7 g) as a pale-brown powder.

(3) Pyridine (1.2 mL) and phosphorus oxychloride (2.1 mL) were added to the above-mentioned compound (2.0 g), and the mixture was stirred at 125°C for 5 hr. Ice was added to the reaction mixture, and the mixture was extracted with chloroform. The extract was washed with water, dried and concentrated under reduced pressure to give 3-chlorobenz[d]isoxazole (1.7 g) as a brown solid.

(4) Using the above-mentioned compound (1.7 g) and piperazine

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CA 02418656 2003-02-06 (7.6 g), and in the same manner as in Example 189 (1), 4-(3benz[d]isoxazolyl)piperazine (0.944 g) was obtained as a gray powder.

(5) Using the title compound (0.900 g) of Reference Example 12 and the above-mentioned compound (0.734 g), and in the same manner as in Example 70 (1), 3-{(2S,4S)-4-[4-(3- ben z[d]isoxa zoly1)-1-pipera z iny1]-1-tert-butoxycarbony1-2pyrrolidinylcarbonyl}-l,3-thiazolidine (1.5 g) was obtained as a white solid.

(6) Using the above-mentioned compound (1.0 g), and in the same manner as in Example 70 (2), the title compound (0.978 g) was obtained as a white powder.

¹H-NMR(DMSO-de )62.19-2.35( lH,m), 2.90-3.16(3H,m), 3.253.55(4H,m), 3.60-4.15(10H,m), 4.47-4.75(4H,m), 7.06-7.12(lH,m),

7.18-7.23(lH,m), 7.36(lH,d,J=7.5Hz), 7.46(lH,d,J=7.8Hz), 9.13(lH,brs), 10.86(1H,brs)

Example 253

Synthesis of 3-{(2S,4S)-4-[4-(5-cyano-3-benz[d]isoxazolyl)-lpipera z iny1]-2-pyrrolidinylcarbony1}-1,3-thia zolidine trihydrochloride (1) Methyl salicylate (149 g) was dissolved in acetic acid (900 mL), and bromine (50 mL) was added thereto. The mixture was stirred at room temperature for 21 hr. Water (10 L) was added to the reaction mixture, and the precipitated solid was collected by filtration, which was then recrystallized from methanol to give methyl 5-bromosalicylate (175 g) as white crystals.

(2) Using the above-mentioned compound (30 g), and in the same manner as in Example 252 (1), 5-bromosalicylhydroxamic acid (24 g) was obtained as white crystals.

(3) Using the above-mentioned compound (10 g), and in the same manner as in Example 252 (2), 5-bromo-3-hydroxybenz[d]isoxazole (8.6 g) was obtained as a white powder.

218

CA 02418656 2003-02-06 (4) Using the above-mentioned compound (8.6 g), and in the same manner as in Example 252 (3), 5-bromo-3-chlorobenz[d]isoxazole (7.4 g) was obtained as a white powder.

(5) Using the above-mentioned compound (7.4 g) and piperazine (21 g), and in the same manner as in Example 189 (1), 4-(5bromo-3-benz[d]isoxazolyl)piperazine (6.5 g) was obtained as a gray powder.

(6) The above-mentioned compound (6.5 g) was dissolved in a solution of tetrahydrofuran (100 mL) and sodium hydroxide (1.0 g) in water (125 mL) and benzyl chloroformate (3.9 mL) was gradually added under ice-cooling. The mixture was stirred at room temperature for 2 hr. The precipitated solid was collected by filtration, and washed with diisopropyl ether to give l-benzyloxycarbonyl-4-(5-bromo-3benz[d]isoxazolyl)piperazine (8.3 g) as a pale-brown powder.

(7) The above-mentioned compound (3.0 g) and zinc cyanide (1.1 g) was dissolved in DMF (15 mL), and tetrakistriphenylphosphinepalladium (0.633 g) was added thereto. The mixture was stirred at 85°C for 18 hr under a nitrogen atmosphere. The reaction mixture was added to 2 mol/L aqueous ammonia and extracted with ethyl acetate. The extract was washed successively with water and brine, dried and concentrated under reduced pressure. The residue was purified by silica gel chromatography to give l-benzyloxycarbonyl-4-(5cyano-3-benz[d]isoxazolyl)piperazine (2.1 g) as a white solid.

(8) The above-mentioned compound (1.5 g) was suspended in methanol (60 mL), and ammonium formate (1.8 g) and 5% palladium carbon (0.230 g) were added thereto. The mixture was refluxed for 0.5 hr. The reaction mixture was filtrated and the filtrate was concentrated under reduced pressure. Saturated aqueous sodium hydrogencarbonate solution was added to the residue, and the mixture was extracted with chloroform. The extract was dried and concentrated under reduced pressure to

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CA 02418656 2003-02-06 give a mixture (1.1 g) containing 4-(5-cyano-3benz[d]isoxazolyl)piperazine as a white solid.

(9) Using the title compound (904 mg) and the above-mentioned compound (681 mg), and in the same manner as in Example 70 (1),

3-{(2S,4S)-l-tert-butoxycarbonyl-4-[4-(5-cyano-3- benz[d]isoxazolyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-l,3thiazolidine (778 mg) was obtained as a white solid.

(10) Using the above-mentioned compound (778 mg), and in the same manner as in Example 70 (2), the title compound (349 mg) was obtained as a white powder.

¹H-NMR(DMSO-d₆ )02.15-2.40 ( lH,m), 2.90-3.20( 3H,m), 3.254.20(14H,m), 4.47-4.77(4H,m), 7.86(lH,d,J=8.7Hz), 8.06(lH,dd,J=8.7,1.2Hz), 8.81(lH,brs), 9.11(lH,brs), 10.65(lH,brs).

Example 254

Synthesis of 3-{(2S,4S)-4-[4-(5-methoxy-3-benz[d]isoxazolyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine tr ihydroc hloride (1) 5-Methoxysalicylic acid (41 g) was dissolved in acetonitrile (500 mL), and ethyl iodide (20 mL) and DBU (38 mL) were added thereto. The mixture was refluxed for 6 hr. The reaction mixture was concentrated under reduced pressure, and 1 mol/L hydrochloric acid was added to the residue. The mixture was extracted with ethyl acetate. The extract was washed successively with saturated aqueous sodium hydrogencarbonate solution and brine, dried and concentrated under reduced pressure to give a mixture (44 g) containing ethyl 5methoxysalicylate as a brown oil.

(2) Using the above-mentioned compound (44 g), and in the same manner as in Example 252 (1), 5-methoxysalicylhydroxamic acid (33 g) was obtained as white crystals.

(3) Using the above-mentioned compound (33 g), and in the same manner as in Example 252 (2), 3-hydroxy-5220

CA 02418656 2003-02-06 methoxybenz[d]isoxazole (5.9 g) was obtained as a pale-brown powder.

(4) Using the above-mentioned compound (5.9 g), and in the same manner as in Example 252 (3), a mixture (4.2 g) containing 3- chloro-5-hydroxybenz[d]isoxazole was obtained as a black oil.

(5) Piperazine (19 g) was melted by heating at 140°C, and the above-mentioned title compound (4.2 g) was added thereto.

After stirring at 140°C for 1 hr, the mixture was poured into iced water and extracted with chloroform. After drying, the io solvent was evaporated under reduced pressure, and the residue was diluted with ethyl acetate and extracted with 1 mol/L hydrochloric acid. The aqueous layer was basified with 2 mol/L aqueous sodium hydroxide solution and extracted with chloroform. After drying, the solvent was evaporated under reduced pressure is to give 4-(5-methoxy-3-benz[d]isoxazolyl)piperazine (804 mg) as a black solid.

(6) Using the title compound (0.900 g) of Reference Example 12 and the above-mentioned compound (0.804 g), and in the same manner as in Example 70 (1), 3-{(2S,4S)-1-tert-butoxycarbonyl-

4-(4-(5-methoxy-3-benz[d]isoxazolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (1.0 g) was obtained as white amorphous.

(7) Using the above-mentioned compound (1.0 g), and in the same manner as in Example 70 (2), the title compound (0.693 g) was obtained as a white powder.

^XH-NMR (DMSO-de )62.20-2.40 (lH,m), 2.90-3.19 ( 3H,m), 3.303.60(4H,m), 3.65-4.15(13H,m), 4.30-4.76(4H,m),

6.65(lH,dd,J=8.7,2.4Hz), 6.97(lH,d,J=2.4Hz), 7.35(1H,d,J=8.7Hz), 10.15(lH,brs), 10.95(lH,brs).

Example. 255

Synthesis of 3-{(2S,4S)-4-[4-(3-benz[d]isothiazolyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine 1.5 oxalate

221

CA 02418656 2003-02-06 (1) Using 1-(3-benz[d]isothiazolyl)piperazine (385 mg) and the title compound (500 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-4-[4-(3- benz[d]isothiazolyl)-1-piperazinyl]-l-tert-butoxycarbonyl-2pyrrolidinylcarbonyl}-l,3-thiazolidine (716 mg) was obtained as a white powder.

(2) The above-mentioned compound (709 mg) was dissolved in methanol (10 mL), and 4 mol/L hydrochloric acid-ethyl acetate (10 mL) was added thereto. The mixture was stirred at room temperature for 19 hr. The precipitated solid was collected by filtration, and saturated aqueous sodium hydrogencarbonate solution was added thereto. The mixture was extracted with chloroform. The extract was concentrated under reduced pressure, and the residue was dissolved in ethanol (10 mL), and oxalic acid (130 mg) was added thereto. The precipitated solid was collected by filtration to give the title compound (150 mg) as a white powder.

¹ H-NMR(DMSO-de)ôl. 60-1.81( lH,m), 2.78-2.85(4H,m), 2.893.24(4H,m), 3.52-3.99(7H,m), 4.43-4.74(4H,m), 7.39-7.48(lH,m),

7.53-7.60(lH,m), 8.01-8.09(2H,m).

Example 256

Synthesis of 3-{(2S,4S)-4-[4-(2-oxazolo[4,5-b]pyridyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-1,3-thiazolidine tr ihydrochloride (1) 2-Amino-3-hydroxypyridine (5.51 g) was dissolved in pyridine (100 mL), and potassium ethylxanthate (8.82 g) was added thereto. The mixture was refluxed for 2 hr. Iced water (400 mL) was added to the reaction mixture, and concentrated hydrochloric acid (40 mL) was added thereto. The mixture was extracted with chloroform and the extract was washed with brine and concentrated under reduced pressure to give 1,3oxazolo[4,5-b]pyridine-2-thiol (5.13 g) as a pale-brown powder.

(2) The above-mentioned compound (5 g) and 2 drops of DMF were

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CA 02418656 2003-02-06 added to thionyl chloride (20 mL). The mixture was refluxed for 1 hr. The reaction mixture was concentrated under reduced pressure to give 2-chloro-l,3-oxazolo[4,5-b]pyridine (5.08 g) as a pale-brown powder.

(3) Using the above-mentioned compound (3.09 g) and piperazine (5.17 g), and in the same manner as in Example 239 (3), 1-(2oxazolo[4,5-b]pyridyl)piperazine (1.15 g) was obtained as a yellowish brown powder.

(4) Using the above-mentioned compound (1.15 g) and the title io compound (0.601 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl-

4-(4-(2-oxazolo[4,5-b]pyridyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (0.635 g) was obtained as a white solid.

is (5) Using the above-mentioned compound (635 mg), and in the same manner as in Example 186 (2), the title compound (293 mg) was obtained as a white powder.

¹H-NMR(DMSO-de)02.02-2.30( 1H,m), 2.83-3.00( lH,m), 3.003.20(2H,m), 3.20-3.48(4H,m), 3.48-4.30(9H,m), 4.40-4.80(3H,m),

7.16(lH,dd,J=7.8,5.4Hz), 7.95(lH,d,J=7.8Hz), 8.22(lH,d,J=5.4Hz), 9.09(lH,brs), 10.59(lH,brs)

Example 257

Synthesis of 3-((2S,4S)-4-{4-[2-(1-ethoxycarbonyl-lmethylethyl)-6-imidazo[l,2-b]pyridazinyl]-l-piperazinyl}-225 pyrrolidinylcarbonyl)-1,3-thiazolidine trihydrochloride (1) Using ethyl 2-(6-chloroimidazo[l,2-b]pyridazin-2-yl)-2methylpropionate (2.00 g) and piperazine (19.7 g), and by a reaction at 100°C in the same manner as in Example 196 (1), 1[2-(1-ethoxycarbonyl-l-methylethyl)-6-imidazo(1,2-

b]pyridazinyl]piperazine (2.37 g) was obtained as an oil.

(2) Using the above-mentioned compound (2.37 g) and the title compound (1.87 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-((2S,4S)-l-tert-butoxycarbonyl223

CA 02418656 2003-02-06

4-(4-(2-(l-ethoxycarbonyl-l-methylethyl)-6-imidazo(1,2-

b]pyrida z iny1]-1-pipera z iny1}-2-pyrrolidinyle arbony1)-1,3thiazolidine (3.29 g) was obtained as a pale-yellow solid.

(3) The above-mentioned compound (1.10 g) was dissolved in dichloromethane (10 mL), and trifluoroacetic acid (5 mL) was added thereto. The mixture was stirred at room temperature for

1.5 hr. The solvent was evaporated under reduced pressure, and the residue was dissolved in ethyl acetate. 4 mol/L Hydrochloric acid-ethyl acetate solution (1 mL) was added thereto, and the precipitated solid was collected by filtration to give the title compound (1.05 g) as a white powder.

¹ H-NMR(500MHz,DMSO-de)Ô1.15(3H,t,J=7.1Hz), 1.61(6H,s), 2.162.36(lH,m), 2.90-4.80(19H,m), 4.10(2H,q,J=7.1Hz),

7.64(lH,d,J=10Hz), 8.10(lH,d,J=10Hz), 8.22(lH,s), 9.11(lH,brs), 10.85(1H,brs).

Example 258

Synthesis of 3-((2S,4S)-4-(4-[2-(l-carboxy-l-methylethyl)-6imidazo[ 1, 2-b]pyridazinyl]-l-piperazinyl}-2pyrrolidinylcarbonyl)-1,3-thiazolidine trihydrochloride (1) 3-((2S,4S)-l-tert-Butoxycarbonyl-4-(4-(2-(l-ethoxycarbonyl1-methylethyl)-6-imida zo[ 1,2-b]pyrida z iny1]-1-pipera z iny1} -2 pyrrolidinylcarbonyl)-1,3-thiazolidine [product of Example 257 (2) , 608 mg] was dissolved in ethanol (2 mL). A solution of lithium hydroxide monohydrate (85 mg) in water (1 mL) was added, and the mixture was stirred at 60°C for 2 hr. Dilute hydrochloric acid was added to the reaction mixture to make the pH 6. The mixture was extracted with chloroform. The extract was dried and concentrated under reduced pressure to give 3- ((2S,4S)-1-tert-butoxycarbonyl-4-(4-[2-(1-carboxy-1methylethy1)-6-imida zo[1,2-b]pyridaziny1]-1-pipera z iny1}-2pyrrolidinylcarbonyl)-1,3-thiazolidine (580 mg) as a paleyellow powder.

(2) Using the above-mentioned compound (579 mg), and in the

224

CA 02418656 2003-02-06 same manner as in Example 257 (3), the title compound (477 mg) was obtained as a white powder.

¹ H-NMR(500MHz,DMSO-de)01.59(6H,s), 2.11-2.31(lH,m), 2.824.20(16H,m), 4.47-4.74(3H,m), 7.64(lH,d,J=10Hz),

8.09(lH,d,J=10Hz), 8.22(lH,s), 9.07(lH,brs), 10.68(lH,brs), 12.85(lH,brs).

Example 259

Synthesis of 3-((2S,4S)-4-[4-(2-methyl-4-quinolyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine trihydrochloride (1) Phosphorus oxychloride (30 mL) was added to 4-hydroxy-2methylquinoline (10 g), and the mixture was stirred at room temperature for 30 min. The reaction mixture was concentrated under reduced pressure, and saturated aqueous sodium carbonate solution was added to the residue under ice-cooling. The mixture was extracted with ethyl acetate. The extract was washed with brine, and concentrated under reduced pressure to give 4-chloro-2-methylquinoline (11.2 g) as a black oil.

(2) Using the above-mentioned compound (5.33 g) and piperazine (25.8 g), and in the same manner as in Example 189 (1), 1-(2methyl-4-quinolyl)piperazine (4.19 g).

(3) Using the above-mentioned compound (500 mg) and the title compound (601 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-((2S,4S)-l-tert-butoxycarbonyl4-(4-(2-methyl-4-quinolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (1.15 g) was obtained as a pale-yellow oil.

(4) Using the above-mentioned compound (1.15 g), and in the same manner as in Example 186 (2), the title compound (0.863 g) was obtained as a pale-brown powder.

^XH-NMR( DMSO-de )02.20-2.42 ( lH,m), 2.81(3H,S), 2.91-3.20 ( 3H,m),

3.30-4.26(13H,m), 4.44-4.87(3H,m), 7.35(1H,S),

7.73{lH,t,J=7.6Hz), 8.00(lH,t,J=7.6Hz), 8.16(lH,d,J=8.4Hz),

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CA 02418656 2003-02-06

8.24(lH,d,J=8.4Hz)

Example 260

Synthesis of 3-((2S,4R)-4-[4-(2-trifluoromethyl-4-quinolyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine dihydrochloride (1) Diethanolamine (20 mL) was added to 4-chloro-2trifluoromethylquinoline (5.04 g), and the mixture was stirred at 80°C for 19 hr. Brine was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The extract was dried and concentrated under reduced pressure. The residue was purified by silica gel chromatography to give 4-[N,N-bis(2hydroxyethyl)amino]-2-trifluoromethylquinoline (3.40 g) as a white powder.

(2) The above-mentioned compound (1.77 g) and triethylamine (2.63 mL) were dissolved in ethyl acetate (100 mL), and methanesulfonyl chloride (1.28 mL) was added under ice-cooling. The mixture was stirred at room temperature for 30 min. The precipitated solid was collected by filtration, and the filtrate was concentrated under reduced pressure to give dimesylate as an oil. This was dissolved in N-methyl-2pyrrolidone (100 mL), and the title compound (2.02 g) of Reference Example 15 and Ν,Ν-diisopropylethylamine (3.10 mL) were added thereto. The mixture was stirred at 100°C for 15 hr. 10% Aqueous citric acid solution was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The extract was washed successively with saturated aqueous sodium hydrogencarbonate solution and brine, dried and concentrated under reduced pressure. The residue was purified by silica gel chromatography to give 3-((2S,4R)-l-tert-butoxycarbonyl-4-[4- (2-trifluoromethyl-4-quinolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (0.248 g) as a palebrown powder.

(3) Using the above-mentioned compound (246 mg), and in the

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¹ H-NMR(500MHz,DMSO-de)02.40-2.50(lH,m), 3.00-3.18(3H,m), 3.593.95(12H,m), 4.12-4.22(lH,m), 4.47-5.03(3H,m), 7.39(lH,s), 7.75(lH,t,J=7.9Hz), 7.88-7.91(lH,m), 8.12-8.14(2H,m),

9.30(lH,brs), 10.75(1H,brs).

Example 261

Synthesis of 3-{(2S,4S)-4-[4-(7-trifluoromethyl-4-quinolyl)-lpipera ziny 1 ] -2 -pyrrolidinylcarbonyl } -1,3-thia zolidine trihydrochloride (1) Using 4-chloro-7-(trifluoromethyl)quinoline (2.5 g) and piperazine (9.30 g), and in the same manner as in Example 189 (1) , l-(7-trifluoromethyl-4-quinolyl)piperazine (3.04 g) was obtained as a pale-brown solid.

(2) Using the above-mentioned compound (619 mg) and the title compound (601 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(7-trifluoromethyl-4-quinolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (458 mg) was obtained as a pale-yellow oil.

(3) Using the above-mentioned compound (458 mg), and in the same manner as in Example 186 (2), the title compound (282 mg) was obtained as a brown powder.

^XH-NMR(DMSO-d₆)02.20-2.47(lH,m), 2.90-3.20(3H,m), 3.304.30(13H,m), 4.45-4.85(3H,m), 7.46(lH,d,J=6.7Hz),

7.98(lH,dd,J=9.0,1.5Hz), 8.43(lH,d,J=8.9Hz), 8.62(1H,S), 8.96(lH,d,J=6.7Hz).

Example 262

Synthesis of 3-{(2S,4S)-4-(4-(2-methoxycarbonyl-4-quinolyl)-1piperazinyl]-2-pyrrolidinylcarbonyl}-1,3-thiazolidine trihydrochloride (1) Phosphorus oxychloride (100 mL) was added to kynurenic acid (25 g). The mixture was refluxed for 6 hr. The reaction

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CA 02418656 2003-02-06 mixture was concentrated under reduced pressure, and the residue was dissolved in 1,4-dioxane (100 mL). Aqueous ammonia (400 mL) was dropwise added thereto under ice-cooling. Water (500 mL) was added thereto, and the mixture was extracted with chloroform. The extract was washed with brine and concentrated under reduced pressure to give 4-chloroquinoline-2-carboxamide (27.3 g) as a black purple solid.

(2) Piperazine (12.9 g) was suspended in N-methyl-2-pyrrolidone (170 mL) and the above-mentioned compound (10.3 g) was added thereto. The mixture was stirred at 80°C with heating. The reaction mixture was concentrated under reduced pressure, and the residue was added to 1 mol/L hydrochloric acid (150 mL), and the mixture was washed with chloroform. The mixture was basified with aqueous sodium hydroxide solution, and the mixture was extracted with chloroform. The extract was concentrated under reduced pressure to give l-(2-carbamoyl-4quinolyl)piperazine (35.18 g) as a pale-yellow powder.

(3) Using the above-mentioned compound (2.54 g) and the title compound (1.26 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(2-carbamoyl-4-quinolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (2.19 g).

(4) The above-mentioned compound (2.19 g) and imidazole (0.551 g) were dissolved in pyridine (20 mL), and phosphorus oxychloride (1.51 mL) was dropwise added to the reaction mixture under ice-cooling. The mixture was stirred under icecooling for 1 hr. Saturated aqueous sodium hydrogencarbonate solution was added to the reaction mixture, and the mixture was extracted with chloroform. The extract was concentrated under reduced pressure and the residue was purified by silica gel chromatography to give 3-&lt;(2S,4S)-l-tert-butoxycarbonyl-4-(4- (2-cyano-4-quinolyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-

1,3-thiazolidine (0.658 g) as a red orange solid.

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27103-383 (5) 5.6 mol/L Hydrochloric acid-methanol (30 mL) was added to the above-mentioned compound (992 mg), and the mixture was stirred at room temperature for 24 hr. The reaction mixture was concentrated under reduced pressure, and saturated aqueous sodium hydrogencarbonate solution was added to the residue.

The mixture was extracted with chloroform and the extract was concentrated under reduced pressure. The residue was purified by silica gel chromatography to give a free base (670 mg) of the title compound as a pale-yellow oil. This was dissolved in io methanol (10 mL), and 5.6 mol/L hydrochloric acid-methanol (0.79 mL) was added thereto. The mixture was concentrated under reduced pressure to give the title compound (615 mg) as a pale-yellow powder.

¹H-NMR(DMSO-d₆)02.20-2.56(lH,m), 2.82-3.20(3H,m), 3.4015 4.30(13H,m), 4.00(3H,s), 4.41-4.82(3H,m), 7.63(lH,s),

7.75(lH,t,J=7.8Hz), 7.93(lH,t,J=7,8Hz), 8.17(lH,d,J=7.8Hz), 8.24(lH,d,J=7.8Hz), 9.20(lH,brs), 11.05(lH,brs).

Example 263

Synthesis of 3-{(2S,4S)-4-[4-(2-carbamoyl-4-quinolyl)-l20 piperazinyl]-2-pyrrolidinylcarbonyl}-l, 3-thiazolidine trihydrochloride

Using 3-{ ( 2S, 4S ) -1-tert-butoxycarbony 1-4-[ 4-( 2-carbamoyl4-quinolyl ) -1-piperazinyl ] -2-pyrrolidinylcarbonyl}-!, 3thiazolidine [product of Example 262 (3), 1.05 g], and in the same manner as in Example 186 (2), the title compound (696 mg) was obtained as a brown powder.

¹ H-NMR ( DMSO-d₆ ) Ô2.20-2.42 ( lH,m), 2.89-3.20 ( 3H,m), 3.304.30(13H,m), 4.44-4.83(3H,m), 7.73(1H,t,J=8.0Hz), 7.87(1H,S),

7.95(lH,t,J=8.0Hz), 8.17(lH,d,J=8.0Hz), 8.24(lH,brs),

8.28(lH,d,J=8.0Hz), 8.86(lH,brs), 9.18(lH,brs), 10.89(lH,brs).

Example 264

Synthesis of 3-{(2S,4S)-4-[4-(3-ethoxycarbonyl-4-quinolyl)-1piperazinyl]-2-pyrrolidinylcarbonyl}-!, 3-thiazolidine

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CA 02418656 2003-02-06 trihydrochloride (1) Diethyl ethoxymethylenemalonate (115 g) was dropwise added to aniline (50 g), and the mixture was refluxed for 1 hr. The resulting ethanol was evaporated under atmospheric pressure, and the residue was heated at 200°C and poured into diphenyl ether (750 mL). The mixture was stirred at 220 - 250°C for 2 hr with heating. The resulting ethanol was again evaporated under atmospheric pressure, and the reaction mixture was allowed to cool to room temperature. The precipitated solid was collected by filtration and washed with hexane to give ethyl 4-hydroxyquinoline-3-carboxylate (51.0 g) as a white powder.

(2) The above-mentioned compound (51.0 g) was added to phosphorus oxychloride (121 mL), and the mixture was stirred at 60 -70°C for 2 hr with heating. The reaction mixture was concentrated under reduced pressure, and the residue was added to iced water (1 L). The mixture was basified with 1 mol/L aqueous sodium hydroxide solution (500 mL) and sodium hydrogencarbonate. The mixture was extracted with ethyl acetate, washed with brine and concentrated under reduced pressure to give ethyl 4-chloroquinoline-3-carboxylate (54.2 g) as a pale-brown solid.

(3) Piperazine (12.9 g) was dissolved in DMF (100 mL), and a solution of the above-mentioned compound (11.8 g) in DMF (100 mL) was added thereto. The mixture was stirred at room temperature for 1 hr. The reaction mixture was added to iced water (500 mL), and the mixture was extracted with ethyl acetate. The extract was washed successively with water and brine, and concentrated under reduced pressure to give 1-(2ethoxycarbonyl-4-quinolyl)piperazine (7.92 g) as a pale-yellow solid.

(4) Using the above-mentioned compound (3.42 g) and the title compound (2.70 g) of Reference Example 12, and in the same

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CA 02418656 2003-02-06 manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(3-ethoxycarbonyl-4-quinolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (4.57 g) was obtained as a pale-yellow powder.

(5) The above-mentioned compound (600 mg) was dissolved in ethanol (20 mL), and 4.1 mol/L hydrochloric acid-ethanol (10 mL) was added thereto. The mixture was stirred at room temperature for 24 hr. The reaction mixture was concentrated under reduced pressure, and saturated aqueous sodium hydrogencarbonate solution was added to the residue. The mixture was extracted with chloroform and the extract was concentrated under reduced pressure. The residue was purified by silica gel chromatography to give a free base (269 mg) of the title compound. This was dissolved in ethanol (5 mL) and

4.1 mol/L hydrochloric acid-ethanol(0.42 mL) was added thereto. The mixture was concentrated under reduced pressure to give the title compound (253 mg) as a pale-yellow powder.

¹H-NMR(DMSO-d₆ )01.41 ( 3H, t, J=6.9Hz ), 2.22-2.46 ( lH,m), 2.903.20(3H,m), 3.30-4.30(13H,m), 4.33-4.82(5H,m), 7.80(lH,t,J=7.8Hz), 8.02(1H,t,J=7.8Hz), 8.19(lH,d,J=7.8Hz), 8.27(lH,d,J=7.8Hz), 9.02(lH,s), 9.15(lH,brs), 10.88(lH,brs).

Example 265

Synthesis of 3-{(2S,4S)-4-[4-(2-cyano-4-quinolyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-1,3-thiazolidine tr ihydroc hloride

3-{(2S,4S)-l-tert-Butoxycarbonyl-4-[4-(2-cyano-4quinoly1)-1-pipera z iny1]-2-pyrrolidinylcarbonyl}-1,3thiazolidine [product of Example 262(4), 658 mg] was dissolved in chloroform (10 mL), and 4 mol/L hydrochloric acid-ethyl acetate (5 mL) was added thereto. The mixture was stirred at room temperature for 24 hr. The solvent was evaporated under reduced pressure, and saturated aqueous sodium hydrogencarbonate solution was added to the residue. The

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CA 02418656 2003-02-06 mixture was extracted with chloroform and the extract was concentrated under reduced pressure. The residue was purified by silica gel chromatography to give a free base (435 mg) of the title compound. This was dissolved in ethanol (20 mL), and

4.1 mol/L hydrochloric acid-ethanol (0.75 mL) was added thereto. The mixture was concentrated under reduced pressure to give the title compound (353 mg) as a pale-yellow powder.

¹H-NMR(DMSO-de)02.20-2.45( lH,m), 2.90-3.20(3H,m), 3.304.22(13H,m), 4.43-4.80(3H,m), 7.63(lH,s), 7.75(1H,t,J=7.8Hz), 7.89(1H,t,J=7.8z), 8.01-8.19(2H,m), 9.17(lH,brs), 10.71(lH,brs). Example 266

Synthesis of 3-{(2S,4S)-4-(4-(2-phenyl-4-quinolyl)-1pipera z iny1]-2-pyrrolidinylcarbony1}-1,3-thia zolidine trihydrochloride (1) Using 4-chloro-2-phenylquinoline (2.50 g) and piperazine (8.98 g), and in the same manner as in Example 268 (1), 1-(2phenyl-4-quinolyl)piperazine (2.72 g) was obtained.

(2) Using the above-mentioned compound (1.01 g) and the title compound (1.00 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl-

4-(4-(2-phenyl-4-quinolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (1.77 g) was obtained as a white powder.

(3) The above-mentioned compound (1.75 g) was dissolved in methanol (10 mL), and 4 mol/L hydrochloric acid-ethyl acetate (10 mL) was added thereto. The mixture was stirred at room temperature for 14 hr. The precipitated solid was collected by filtration to give the title compound (0.970 g) as a white powder.

¹ H-NMR(DMSO-de)02.20-2.45( lH,m), 2.90-3.17(3H,m), 3.284.00(13H,m), 4.42-4.80(3H,m), 7.57(lH,s), 7.61-7.82(4H,m),

7.95-8.08(lH,m), 8.16-8.31(3H,m), 8.50(lH,d,J=8.7Hz),

9.15(lH,brs), 11.06(lH,brs).

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Example 267

Synthesis of 3-{(2S,4S)-4-[4-(2-amino-4-quinolyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-l,3-thiazolidine tr ihydroc hloride (1) Bromine (2.32 mL) was dissolved in 5% aqueous potassium hydroxide solution (190 mL), and a solution of 4chloroquinoline-2-carboxamide [product of Example 262 (1), 9.80 g], in tetrahydrofuran (190 mL) was dropwise added thereto. The mixture was stirred at room temperature for 30 min, and the mixture was stirred at 80°C for 1 hr with heating. The reaction mixture was filtrated and the filtrate was added to water. The mixture was extracted with chloroform, and the extract was washed with brine and concentrated under reduced pressure. The residue was purified by silica gel chromatography to give 2-amino-4-chloroquinoline (1.98 g) as a pale-yellow solid.

(2) Using the above-mentioned compound (1.98 g) and piperazine (19.1 g), and in the same manner as in Example 189 (1), 1-(2amino-4-quinolyl)piperazine (1.85 g) was obtained as a palebrown powder.

(3) Using the above-mentioned compound (913 mg) and the title compound (901 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-[4-(2-amino-4-quinolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (465 mg) was obtained as a white solid.

(4) Using the above-mentioned compound (465 mg), and in the same manner as in Example 186 (2), the title compound (439 mg) was obtained as a white powder.

¹H-NMR(DMSO-d₆)02.20-2.42(lH,m), 2.86-3.20( 3H,m), 3.304.30(13H,m), 4.41-4.84(3H,s), 6.52(1H,S), 7.46(lH,t,J=8.1Hz), 7.59-7.82(2H,m), 7.91(lH,d,J=8.1Hz), 8.33(2H,brs), 9.14(lH,brs) 10.85(1H,brs), 13.79(1H,brs)

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Example 268

Synthesis of 3-{(2S,4S)-4-[4-(7-chloro-4-quinolyl)-lpiperazinyl]-2-pyrrolidinylcarbonyl}-]., 3-thiazolidine trihydrochloride (1) Piperazine (65.2 g) was melted by heating at 120°C, and

4,7-dichloroquinoline (15.0 g) was added thereto. The mixture was stirred at 120°C for 2.5 hr and the reaction mixture was added to water. The mixture was extracted with chloroform and concentrated under reduced pressure to give 1-(7-chloro-4quinolyl)piperazine (7.72 g).

(2) Using the above-mentioned compound (0.87 g) and the title compound (1.00 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-(4-(7-chloro-4-quinolyl)-l-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (1.65 g) was obtained as a white powder.

(3) The above-mentioned compound (1.64 g) was suspended in methanol (10 mL), and 4 mol/L hydrochloric acid-ethyl acetate (8.0 mL) was added thereto. The mixture was stirred at room temperature for 13 hr. The precipitated solid was collected by filtration to give the title compound (1.04 g) as a white powder.

¹ H-NMR(DMSO-de)Ô2.10-2.37( lH,m), 2.84-4.00( 16H,m) , 4.414.82(3H,m), 7.36(lH,d,J=6.9Hz), 7.77(lH,dd,J=9.0,1.8Hz), 8.22(lH,d,J=9.0Hz), 8.27(lH,d,J=1.8Hz), 8.85(lH,d,J=6.9Hz), 9.18(lH,brs), 10.82(lH,brs).

Example 269

Synthesis of 3-{(2S,4S)-4-[4-(2-trifluoromethyl-8-methyl-4quinolyl)-l-piperazinyl]-2-pyrrolidinylcarbonyl}-1,3thiazolidine dihydrochloride (1) 2-Methylaniline (5.00 g) was dissolved in 75% phosphoric acid (20 mL), and ethyl trifluoroacetoacetate (8.60 g) was dropwise added thereto at 105°C. The mixture was stirred at

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105°C for 5.5 hr. After allowing to cool, the reaction mixture was added to water. The precipitated solid was collected by filtration to give 2-trifluoromethyl-4-hydroxy-8methylquinoline (1.84 g).

(2) The above-mentioned compound (1.82 g) was dissolved in phosphorus oxychloride (8.00 mL), and the mixture was stirred at 70°C for 2.5 hr. The reaction mixture was concentrated under reduced pressure, and saturated aqueous sodium hydrogencarbonate solution was added to the residue. The mixture was extracted with chloroform and the extract was concentrated under reduced pressure to give 4-chloro-2- trifluoromethyl-8-methylquinoline (1.66 g ).

(3) Using the above-mentioned compound (1.65 g) and piperazine (4.00 g), and in the same manner as in Example 268 (1), 1-(2trifluoromethyl-8-methyl-4-quinolyl)piperazine (1.57 g) was obtained.

(4) Using the above-mentioned compound (1.03 g) and the title compound (1.00 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-((2S,4S)-l-tert-butoxycarbonyl4-(4-(2-trifluoromethyl-8-methyl-4-quinolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (1.81 g) was obtained as a white powder.

(5) Using the above-mentioned compound (1.80 g), and in the same manner as in Example 268 (3), the title compound (1.30 g) was obtained as a white powder.

¹ H-NMR(DMSO-d₆)62.20-2.45( lH,m), 2.72(3H,S), 2.90-3.20(3H,m),

3.25-4.25(13H,m), 4.44-4.82(3H,m), 7.40(lH,s), 7.63(lH,dd,J=8.3,6.9Hz), 7.75(lH,d,J=6.9Hz), 7.93(lH,d,J=8.3Hz), 9.12(lH,brs), 10.85(1H,brs), 12.65(1H,brs).

Example 270

Synthesis of 3-((2S,4S)-4-(4-(2,6-bis(trifluoromethyl)-4quinolyl]-l-piperazinyl}-2-pyrrolidinylcarbonyl)-l,3thiazolidine dihydrochloride

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CA 02418656 2003-02-06 (1) 4-Hydroxy-2,6-bis(trifluoromethyl)quinoline (1.28 g) was dissolved in phosphorus oxychloride (5.0 mL), and the mixture was stirred at room temperature for 17 hr. The reaction mixture was concentrated under reduced pressure, and saturated aqueous sodium hydrogencarbonate solution was added to the residue. The mixture was extracted with chloroform and concentrated under reduced pressure to give 4-chloro-2,6bis(trifluoromethyl)quinoline (1.17 g).

(2) Using the above-mentioned compound (1.14 g) and piperazine io (3.29 g), and in the same manner as in Example 189 (1), 1-(2,6- bis(trifluoromethyl)-4-quinolyl]piperazine (870 mg) was obtained.

(3) Using the above-mentioned compound (0.860 g) and the title compound (0.86 g) of Reference Example 12, and in the same is manner as in Example 70 (1), 3-((2S,4S)-l-tert-butoxycarbonyl4-(4-(2,6-bis( trifluoromethyl)-4-quinolyl]-l-piperazinyl}-2pyrrolidinylcarbonyl)-1,3-thiazolidine (1.55 g) was obtained as a white powder.

(3) Using the above-mentioned compound (1.54 g), and in the same manner as in Example 268 (3), the title compound (860 mg) was obtained as a white powder.

¹ H-NMR(DMSO-de)Ô2.11-2.46( lH,m), 2.72-4.20( 16H,m) , 4.354.89(3H,m), 7.54(1H,S), 8.14(lH,dd,J=9.0,1.2Hz), 8.33(lH,d,J=9.0Hz), 8.38(lH,d,J=1.2Hz), 9.24(lH,bts),

10.88(1H,brs).

Example 271

Synthesis of 3-((2S,4S)-4-(4-(2,8-bis(trifluoromethyl)-4qu inoly1]-1-pipera z iny1}-2-pyrrolidinylcarbony1)-1,3thiazolidine dihydrochloride (1) Using 4-chloro-2,8-bis(trifluoromethyl)quinoline (0.500 g) and piperazine (7.19 g), and in the same manner as in Example 189 (1), 1-(2,8-bis(trifluoromethyl)-4-quinolyl]piperazine (0.519 g) was obtained as a pale-brown solid.

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27103-383 (2) Using the above-mentioned compound (519 mg) and the title compound (406 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)~l-tert-butoxycarbonyl4-(4-(2,8-bis(trifluoromethyl)-4-quinolyl]-l-piperazinyl}-2- pyrrolidinylcarbonyl}-!,3-thiazolidine (631 mg) was obtained as a white powder.

(3) Using the above-mentioned compound (547 mg), and in the same manner as in Example 186 (2), the title compound (362 mg) was obtained as a white powder.

¹H-NMR(DMSO-d_fi)02.11-2.45( lH,m), 2.86-3.20( 3H,m) , 3.304.30(13H,m), 4.45-4.86(3H,m), 7.52(lH,s), 7.85(1H,t,J=8-4Hz), 8.31(lH,d,J=8.4Hz), 8.42(1H,d,J=8.4Hz).

Example 272

Synthesis of 3-((2S,4S)-4-(4-(2-trifluoromethyl-6-methoxy-415 quinolyl)-l-piperazinyl]-2-pyrrolidinylcarbonyl}-l,3thiazolidine trihydrochloride (1) 4-Methoxyaniline (5.00 g) was dissolved in 75% phosphoric acid (20 mL) and ethyl trifluoroacetoacetate (7.48 g) was dropwise added thereto at 110°C. The mixture was stirred at

110°C for 4 hr with heating. After allowing to cool, the reaction mixture was added to saturated aqueous sodium hydrogencarbonate solution, and the mixture was extracted with ethyl acetate. The extract was dried and the solvent was evaporated under reduced pressure. The residue was purified by .25 silica gel chromatography to give 2-trifluoromethy 1-4-hydroxy-

6-methoxyquinoline (0.450 g).

(2) Using the above-mentioned compound (450 mg), and in the same manner as in Example 270 (1), 4-chloro-2-trifluoromethy 16-methoxyquinoline (410 mg) was obtained as a white powder.

(3) Using the above-mentioned compound (0.410 g) and piperazine (1.32 g), and in the same manner as in Example 189 (1), 1-(2trifluoromethyl-6-methoxy-4-quinolyl)piperazine (0.450 g) was obtained.

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CA 02418656 2003-02-06 (4) Using the above-mentioned compound (450 mg) and the title compound (430 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-[4-(2-trifluoromethyl-6-methoxy-4-quinolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (660 mg) was obtained as a white powder.

(5) Using the above-mentioned compound (660 mg), and in the same manner as in Example 268 (3), the title compound (290 mg) was obtained as a white powder.

¹ H-NMR(DMSO-de)02.34-2.54( lH,m), 2.93-3.21(3H,m), 3.314.30(13H,m), 3.97(3H,s), 4.45-4.85(3H,m), 7.31(lH,d,J=2.7Hz), 7.38(1H,S), 7.55(lH,dd,J=9.3,2.7Hz), 8.06(lH,d,J=9.3Hz), 9.18(lH,brs), 11.84(lH,brs), 12.78(lH,brs).

Example 273

Synthesis of 3-{(2S,4S)-4-[4-(2-trifluoromethyl-7-methoxy-4quinolyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-1,3thiazolidine dihydrochloride (1) Using 3-methoxyaniline (20.0 g) and ethyl trifluoroacetoacetate (29.9 g), and in the same manner as in Example 269 (1), 2-trifluoromethyl-4-hydroxy-7-methoxyquinoline (860 mg) was obtained.

(2) Using the above-mentioned compound (850 mg), and in the same manner as in Example 269 (2), 4-chloro-2-trifluoromethyl-

7-methoxyquinoline (820 mg) was obtained as a white powder.

(3) Using the above-mentioned compound (0.820 g) and piperazine (4.00 g), and in the same manner as in Example 189 (1), 1-(2trifluoromethyl-7-methoxy-4-quinolyl)piperazine (0.840 g) was obtained.

(4) Using the above-mentioned compound (0.830 g) and the title compound (0.800 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl4-[4-(2-trifluoromethyl-7-methoxy-4-quinolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-!,3-thiazolidine (1.25 g) was obtained as

238

CA 02418656 2003-02-06 a white powder.

(5) Using the above-mentioned compound (1.24 g), and in the same manner as in Example 268 (3), the title compound (0.900 g) was obtained as a white powder.

¹ H-NMR ( DMSO-de )δ2.28-2.48 ( lH,m), 2.95-3.20 ( 3H,m), 3.213.97(12H,m), 3.95(3H,s), 4.03-4.24(lH,m), 4.44-4.83(3H,m), 7.25(lH,s), 7.36(lH,dd,J=9.9,2.6Hz), 7.51(lH,d,J=2.6Hz), 8.04(lH,d,J=9.9Hz), 9.16(lH,brs), 11.69(lH,brs).

Example 274

Synthesis of 3-((2S,4S)-4-[4-(2-trifluoromethyl-8-methoxy-4quinolyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-1,3thiazolidine dihydrochloride (1) Using 2-methoxyaniline (10.0 g) and ethyl trifluoroacetoacetate (14.5 g), and in the same manner as in Example 269 (1), 2-trifluoromethyl-4-hydroxy-8-methoxyquinoline (2.03 g) was obtained.

(2) Using the above-mentioned compound (2.00 g), and in the same manner as in Example 269 (2), 4-chloro-2-trifluoromethy1-

8-methoxyquinoline (1.95 g) was obtained as a white powder. Using the above-mentioned compound (1.95 g) and piperazine (8.00 g), and in the same manner as in Example 189 (1), 1-(2trifluoromethyl-8-methoxy-4-quinolyl)piperazine (2.14 g) was obtained.

(4) Using the above-mentioned compound (1.60 g) and the title compound (1.01 g) of Reference Example 12, and in the same manner as in Example 70 (1), 3-((2S,4S)-l-tert-butoxycarbonyl4-(4-(2-trifluoromethyl-8-methoxy-4-quinolyl)-1-piperazinyl]-2pyrrolidinylcarbonyl}-l,3-thiazolidine (1.01 g) was obtained as a white powder.

(5) Using the above-mentioned compound (1.01 g), and in the same manner as in Example 268 (3), the title compound (0.640 g) was obtained as a white powder.

¹ H-NMR(DMSO-de)δ2 · 15-2.39( lH,m), 2.80-3.19(3H,m), 3.20239

CA 02418656 2003-02-06

4.18(13H,m), 3.99(3H,s), 4.40-4.78(3H,m), 7.29-7.33(lH,m),

7.35(lH,s), 7.60-7.69(2H,m), 9.14(lH,brs), 10.71(lH,brs),

12.61(lH,brs).

Example 275

Synthesis of 3-{(2S,4S)-4-(4-(8-fluoro-2-trifluoromethy1-4quinolyl)-1-piperazinyl]-2-pyrrolidinylcarbonyl}-1,3thiazolidine dihydrochloride (1) 2-Fluoroaniline (10.0 g), ethyl trifluoroacetoacetate (16.6 g) and concentrated hydrochloric acid (0.1 mL) were dissolved io in benzene (40 mL), and the mixture was refluxed in a reaction vessel equipped with Dean-Stark trap for 7 hr. The reaction mixture was concentrated under reduced pressure and 75% phosphoric acid (40 ml) was added thereto. The mixture was stirred at 110°C for 5 hr. The reaction mixture was added to is water, neutralized with sodium hydrogencarbonate, and extracted with ethyl acetate. The extract was concentrated under reduced pressure to give 8-fluoro-4-hydroxy-2-trifluoromethylquinoline (1-77 g).

(2) Using the above-mentioned compound (1.77 g), and in the same manner as in Example 269 (2), 4-chloro-8-fluoro-2trifluoromethylquinoline (1.70 g) was obtained as a white powder.

(3) The above-mentioned compound (1.45 g), piperazine (0.5 g) and Ν,Ν-diisopropylethylamine (0.751 g) were dissolved in DMF (25 mL), and the mixture was stirred at 70°C for 6.5 hr. The reaction mixture was added to water, and the mixture was extracted with chloroform. The extract was concentrated under reduced pressure and the residue was purified by silica gel chromatography to give 1-(8-fluoro-2-trifluoromethyl-430 quinolyl)piperazine (0.187 g).

(4) Using the above-mentioned compound (186 mg) and the title compound (187 mg) of Reference Example 12, and in the same manner as in Example 70 (1), 3-{(2S,4S)-l-tert-butoxycarbonyl240

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