Nova Patents
ATE63002T1

Assay method employing fluorescent cell incorporative dye

Abstract

A method is provided for determining the presence in a sample of a member of a specific binding pair ("sbp member") consisting of ligand and its homologous receptor. The sample is combined in an aqueous medium with (1) a complementary sbp member wherein at least the sbp member or the complementary sbp member is bound to the surface of a cell and (2) a fluorescent agent capable of being incorporated into the cell. The presence of the sbp member is indicated by a change in fluorescence of the unseparated cell suspension as a result of agglutination of the cells.The present invention has particular application to blood typing, for example, for the determination of the presence of blood group antigens A, B, AB, 0, and D (Rh<sub>o</sub>) and antibodies to such antigens.

Term

Term ended

Projected expiry passed 27 August 2005, 21.1 years ago.

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25 members in 8 offices

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Priority claims
DocumentOfficeKindDate
64545884United States of AmericaA

Members25

Family members, oldest first
DocumentOfficeKind
AU4682085AAustraliaA
JPS6162862AJapanA
EP0176252A2European Patent Office (EPO)A2
EP0176252A3European Patent Office (EPO)A3
US4748129AUnited States of AmericaA
CA1261742ACanadaA
AU591636B2AustraliaB2
EP0356173A2European Patent Office (EPO)A2
JPH02255865AJapanA
EP0176252B1European Patent Office (EPO)B1
AT63002TAustriaT
ATE63002T1This recordAustriaT1
DE3582643D1GermanyD1
US5026905AUnited States of AmericaA
EP0356173A3European Patent Office (EPO)A3
US5145774AUnited States of AmericaA
JPH0692971B2JapanB2
EP0356173B1European Patent Office (EPO)B1
AT122372TAustriaT
ATE122372T1AustriaT1
DE68922548D1GermanyD1
ES2073440T3SpainT3
DE68922548T2GermanyT2
CA1339140CCanadaC
JP2793850B2JapanB2