Method for releasing rna and dna from cells
Abstract
(57) A summary and composition How to put in the solution or suspension of a cell in the container in which a fixed quantity of diameters contain the bead of 0.05*1.0 mm, to make the ultrasonic wave for which sufficient time and power density do not exceed 0.2 W/ml act, crush a cell, and emit RNA and DNA in this cell to solution. Effect RNA and DNA are extractable from a cell in the state where molecular structure is good and it was held, so that an object living thing can be identified and authorized by the hybridization using a heredity probe. Moreover, it can extract, without destroying RNA and DNA too much also from the microbe of recalcitrance like tubercle bacillus.
Term
No projected expiry on record.
- Priority
- Filed
- Granted
- Today
14 members in 7 offices
Priority claims1
| Document | Office | Kind | Date |
|---|---|---|---|
| 84186086 | United States of America | A |
Members14
| Document | Office | Kind | |
|---|---|---|---|
| AU7040487A | Australia | A | |
| JPS62236478A | Japan | A | |
| EP0288618A1 | European Patent Office (EPO) | A1 | |
| AU604684B2 | Australia | B2 | |
| CA1296604C | Canada | C | |
| JPH0568553A | Japan | A | |
| JPH0528599B2 | Japan | B2 | |
| EP0288618B1 | European Patent Office (EPO) | B1 | |
| AT91708TThis record | Austria | T | |
| ATE91708T1 | Austria | T1 | |
| DE3786661D1 | Germany | D1 | |
| DE3786661T2 | Germany | T2 | |
| US5374522A | United States of America | A | |
| JPH0813272B2 | Japan | B2 |
2 legal events, as the office reported them to INPADOC
Over the term
Point at a mark for the eventEvents
| Event | Code | |
|---|---|---|
| Cancelled due to lapse of timeLapsedEELA | EELA | |
| Publication of translation of european patent specificationUEP | UEP |
Numbers
- Application
- 87303641
Titles2
- German
- VERFAHREN ZUR FREISETZUNG VON RNS UND DNS AUS ZELLEN.
- English
- METHOD FOR RELEASE OF RNA AND DNA FROM CELLS.
Classification
- CPC, 5
- C12P19/34
- C12N1/066
- C12Q1/6806
- C12Q1/6832
- C12Q1/689
- IPC, 13
- C12M1 42
- C12N1 00
- C12N1 06
- C12N1 20
- C12N13 00
- C12N15 00
- C12N15 09
- C12P19 34
- C12Q1 04
- C12Q1 68
- C12R1 32
- G01N33 483
- G01N33 50