Production of thrombin inhibitors.

(57) A summary and the purpose The yeast expression vector which can make mature デスルファト hirudin secrete in a culture medium is offered. Composition Yeast revelation control array, it being in the second DNA arrangement that carries out a code, and its upper stream, and mature デスルファト hirudin. What is a DNA segment which consists of the first DNA arrangement that carries out the code of yeast PH05 or invertase signal peptide whose it and reading frame suit, and is under transfer control of the above-mentioned revelation control array, The yeast hybrid vector which has one or more DNA insertions whose DNA arrangement containing the transfer termination signal of yeast each contains.