Entrapped binding proteins as biosensors

The interacting protein in which the present invention was caught, variation interacting protein containing a reporter machine, It is aimed at these use as an analysis thing biosensor in both the composite which has variation interacting protein containing the reporter machine in an analysis thing permeability matrix, in vitro, and in vivo. The covalent bond of the interacting protein is carried out to fluorescein dye, therefore the concentration of the analysis thing (for example, glucose) in a given sample is proportional to the size of a fluorescence signal.