Two step lateral flow assay methods and devices

For detecting comprises multiple non-inductive specific union's sample specific combined to the object to member's first side counter current sensor apparatus and method suitable to two ensurrings. The first embodiment, wherein IgE antibody the two side counter current measuring method for recognizing sample comprises a sample applying to a sample port, device suitable to sample transportation to the first base with multiple fixed on the IgE vision antigen type side counter current matrix. The farther method in comprising an samples block along the side counter current matrix by multiple IgE antigen is fixed to the first and second mounted, a liquid buffer applying to the side counter current matrix of the movable the reagent, the reagent suitable to the waterproof IgE antibody, and sample transportation of the process filter to the side counter current matrix upstream of dry on the side counter current matrix frame, block to adopt the multiple IgE antigen type through the liquid buffer move's the reagent along the side counter current matrix with a are fixed to the first seat is mounted. Is embodiment comprising complimentary side counter current immunoassay sensing device and a IgE antibody method for recognizing sample.