Device for in vivo micro-invasive investigation comprising a metal guide

The invention claims an analysis device wherein said system comprises at least one metal guide device its one end is set with at least one group of hole the hole directly with the substrate having specificity the reaction of the combination of the end of it is the through hole end and the other end is used for controlling the said guide apparatus and it is unnecessary to ground and a suction system is connected with the. Said guiding device can be inserted into the functional end of the level of the detachable protective system in a vertical to the micro quantitative analysis and/or small amounts of sample sites and/or said guiding device can be inserted into having at least the inside of the circuit of medical appliance wherein said guiding device can be used in the sliding. The invention also claims the device in the manufacture of this invention claims a method for diagnosis in the patient cancer infection inflammation of neurodegenerative diseases and graft rejection tool for use in the optimized through the wall of the path. The invention also claims a method for using the device outside the body of analyzing substrate method for.