Methods for producing preparations of recombinant AAV virions substantially free of empty capsids

[Subject] Remove empty capsid from an AAV vector particle stock, or decrease the number of empty capsids, and provide a new method of reinforcing manufacturing capacity.<br />[Means for Solution] The present invention is based on having developed a realizable method efficiently and commercially, in order to prepare a rAAV billion stock in which quantity of empty capsid decreased. In this specification, these artificers found out that it might dissociate from a rAAV billion ("AAV vector particles") containing a genetic material, when empty capsid used column chromatography art. A method for separating empty capsid of AAV from a mixture of AAV vector particles and empty capsid of AAV is indicated. Column chromatography art is used for this method, and it provides a recombination AAV billion of a realizable level commercially.<br />[Chosen drawing] Nothing