Product quality enhancement in mammalian cell culture processes for protein production

Described by the animal cell or a mammalian cell, and illustration, comprising, but not limit to about the material is in a cell culture producing protein, specifically the method and process of the glycoprotein. A method for the d-galactose; the d-galactose from the feeding medium, preferably per day fed to the culture cell, to maintain a wherein in a culture medium spittle the acidification intermediate layer of d-galactose, wherein the added acidification spittle of protein has. The invention further - comprising culturing time, wherein at least two dye solution a, the culturing time when the temperature is lower than the initial cell culture temperature. Maintaining the high survivability cell comprising a cell culture method of the invention twice or multiple in temperature, and can permit the rotating the protein productbility phase. The method further - comprising a time and after adding the poly anionic compound. A d-galactose; the d-galactose and culture medium in the feeding medium to maintain of d-galactose from the culture medium in the acidification spittle intermediate layer comprises the catalyst can until the culture of the reverse is a acidification spittle of the culture enlarged, and is beneficial for large the culture method.