Novel cytokine-like protein

[Subject] Offer of new cytokine Mr. Protein and its gene. [Solution means] The arrangement of EST (AA418955) homology with other protein is not indicated to be at all on the data base finds out that G-CSF and weak homology are shown, As a result of compounding a primer based on that arrangement and performing PCR cloning from a human embryo spleen library, the full length cDNA corresponding to this EST is isolated. Moreover, as a result of determining the base sequence of cDNA which isolated and analyzing the structure, CDNA which isolated has the typical feature as a factor belonging to an IL-6/G-CSF/MGF family. Moreover, the culture supernatant of the CHO cell into which this gene which isolated was introduced has the activity which supports multiplication of marrow cells under coexistence of a kit ligand. [Selection figure] Nothing