Expression of endogenous genes by non-homologous recombination of a vector construct with cellular dna

The invention relates generally by in-situ recombinant method of activated the gene or expression of the gene expression. The invention further relates generally wherein the endogenous gene is higher than the level of the normal cell is represented by to conduct expression in a cell method. A separate an embodiment of the invention, wherein the expression of the cell, is activated the endogenous gene expression of the gene expression one of said control series's the regulatory sequence non-homology or the recombinant illegal; An or improving the expression of the gene endogenous. The other of solution, one or more to marker is selected by the integrated on the expression carrier the copy number of at more than one or according to of the label, that further improving to the expression of the gene endogenous. The invention furthermore provide the identification and expressing a method for the gene of the technology unable to discover use of a, the integrated without target sequence. The invention also provides a separation of coding of the protein the nucleic acid molecule (of the cDNA molecule), and separable energy of such a it is a transverse heterologous protein film of the protein the method for cell. The present invention also relates to the isolated gene and gene product associated nucleic acid molecule and containing the gene and gene product associated nucleic acid molecule, wherein the composition can be used for more and treatments for diagnosis. Therefore, the invention, wherein the activated and separating the endogenous gene, comprising a layer of human growth and related gene, and does not need to any wherein the gene of the sequences, structures, function or the expression.