Methods and materials for making and using transgenic dicamba-degrading organisms

The dicamba degradative enzyme which the present invention isolated and was refined partially at least, Some the transgenic plants and plants containing the transgenics host cell containing the isolation DNA molecule which carries out the code of the dicamba degradative enzyme, the DNA structure which carries out the code of the dicamba degradative enzyme, and DNA which carries out the code of the dicamba degradative enzyme, and one or more cells containing DNA which carries out the code of the dicamba degradative enzyme are offered. The present invention offers further the transportation peptide which is used into the DNA structure containing such dicamba degradative enzyme and which is conveyed to the intracellular smallness organ of a plant cell for the above-mentioned dicamba decomposition オキシゲナーゼ. The living thing object of dicamba resolvability, such as a dicamba tolerance plant, is produced by revelation of dicamba degradative enzyme. The present invention offers further the decontamination method of a dicamba content substance including the process of applying the transgenics microbe or dicamba degradative enzyme of the method of controlling weeds by the field containing the transgenics dicamba tolerance plant of the present invention, and the present invention of an effective quantity to a substance. The way the present invention finally chooses the plant and plant cell which were transformed based on dicamba tolerance, And the method of choosing or screening some of 3 generated by disassembly of dicamba, host cells transformed based on the fluorescence of 6* ジクロロ salicylic acid, whole living thing objects, and living thing objects is offered.