Apparatus for mixing and/or testing small volumes of fluids

An apparatus (10) for measuring, mixing and / or analysis of a series of small volumes of fluids with a high degree of precision, said apparatus (10): a) a single chamber (20) reaction and a base (40 ); b) a piston (30) reciprocating in said chamber (20); c) a first inlet (41) to said chamber (20) in communication with one or more fluid sources of which a portion of a first fluid of said one or more fluids is adapted to be drawn into said chamber ( twenty); d) at least one other input (43) including a second inlet to said chamber (20) in communication with one or more fluid sources of which a portion of a second fluid of said one or more fluids is adapted to be drawn into inside said chamber (20); e) an outlet (45) expandable, wherein f) said piston (30) is operable within said chamber (20) to vary, at will and precisely, the internal volume of said chamber (20); g) said first inlet (41) has a first valve (42) operable to permit flow of said first portion into said chamber (20) by movement of said piston (30) within said chamber (20 ) away from said base (40) until a predetermined volume of said first fluid is drawn into said chamber (20); h) said second inlet (43) has a second valve (44) operable to permit flow of the second portion into said chamber (20) by movement of said piston (30) within said chamber (20 ) to progressively draw said second fluid into said chamber (20) until a predetermined condition, including compliance precisely measuring a volume of said second fluid or the completion of a reaction involving said first and second parts said chamber (20); i) I said piston (30) is adapted to return to its starting position and empty the contents of said chamber (20) through said outlet (45) until all of the sample is ejected through the outlet (45 ), wherein said outlet (45) is closed sealingly by a valve (47) outlet, wherein said valve (47) outlet is located in said base (40) adjacent to the inner surface of said chamber (20) .