Quantified fluorescence microscopy

(57) Summary book invention relates to the proofreading equipment, the proofreading method, and proofreading tool which are used by a microscopic examination method. The proofreading tool for fluorescence microscopy is equipped with the thin opaque mask of the non-fluorescent substance which specifies the reference form opening which has a solid surface containing a support medium and a fluorescent substance and the selected size which exposes a surface portion. The 1st model of this proofreading tool can be equipped with the adhesion promoter who promotes contact between the surface of a support medium, and the solid surface containing a fluorescent substance. This touches the thin opaque mask. The 2nd model of this proofreading tool has a solid surface containing the thin (had an adhesion promoter or it does not have him) opaque mask assembled on the support medium, and the fluorescent substance installed on this opaque mask.