Element, method and kit for the determination of an analyte in a fluid

The invention is an element for determining an analyte in a liquid by means of a specific binding reaction of two bioaffinity binding partner, DOLLAR A containing in or on material which enables liquid transport between zones, a sample application Stone (1) and a thereto located downstream detection zone (4) DOLLAR a and between the sample application zone (1) and detection zone (4) a zone (3) with immobilized analyte or analyte DOLLAR a and upstream of the zone (3) with immobilized analyte or analyte releasably impregnated by liquid conjugate 1 from a to be determined with the analyte to a specific binding reaction capable characterized bioaffine binding partner 1 and a detectable label 1, DOLLAR a that DOLLAR a, the detectable label 1 is a low molecular weight organic molecule is DOLLAR a and upstream of the zone (3) containing immobilized analyte or analyte analogue also releasable by fluid, universal conjugate 2 is available from a with the detectable label 1 capable of a specific binding reaction bioaffine binding partner 2 and a visually detectable marker 2nd DOLLAR A subject of the invention is also the use of an element according to the invention for the determination of an analyte and a kit for determining an analyte containing an element according to the invention and an eluent.