Separation of active complexes

(57) Summary book invention Lipid, cationic lipid, リポソーム, peptide, From the complex formed by the meeting with the transformer フェクティング ingredient and polynucleotide to which transfection activity like cationic peptide, a dendrimer, or the poly cation is made to increase, The active complex with clear composition which has the specific physicochemical feature like density, surface charge, or grain size is separated. In a desirable mode, super-centrifugality of the polynucleotide * transformer フェクティング ingredient complex is carried out, and one or more bands corresponding to the complex which has a specific polynucleotide * transformer フェクティング ingredient interaction are disassembled. The polynucleotide complex which has a cationic liposome transformer フェクティング ingredient is disassembled into two main bands corresponding to the formed complex under a superfluous lipid condition or superfluous polynucleotide conditions. In another mode, it is decomposed using an intersection flow electrophoresis, and a polynucleotide * transformer フェクティング ingredient complex has a complex which has a specific interaction identified, and is separated from a superfluous first ingredient.