Binary probe and clamp composition and methods for target hybridization detection

[Subject] A probe and the binary composite of a clamp perform the method for target hybridization detection. [Solution means] When a probe is a substrate for エキソ nuclease cutting, a composite offers quantification and detection of a PCR product by real-time measurement and terminal point measurement. When a probe is an amplification primer, a composite offers how to have been improved for the sign of a PCR product, and detection. The sign of a probe and the clamp may be carried out using fluorescence pigment, a quencher, a hybridization stabilization portion, chemistry luminescent color matter, and an affinity ligand. A clamp may be a nucleic acid analog like 2* amino ethyl glycine PNA. [Selection figure] Nothing