Medium and matrix for long-term proliferation of cells

(57) The cell culture culture medium and hydrogel substrate for long-term preservation of a summary cell and multiplication are offered. A cell culture culture medium and a hydrogel substrate may also contain an effective amount of polar amino acid chosen from the group which consists of arginine, lysine, histidine, glutamic acid, and aspartic acid. One example of a cell culture culture medium contains the polar amino acid of abbreviation 5* abbreviation 150mM. A hydrogel substrate contains the polar amino acid of abbreviation 3* abbreviation 150mM. Preferably, L* arginine and L* glutamic acid are supplied to a cell culture culture medium. Preferably, L* arginine, L* lysine, and L* glutamic acid are supplied to a hydrogel substrate. The method of maintaining the survival power and the function of the piece of a transplant is also offered. The method of maintaining the survival power of the piece of a transplant contains the step which encloses a cell with a hydrogel substrate, and the step which injects the enclosed cell into a host living thing. Before injecting the substrate of the present invention into a cell, also in order to promote the blood vessel rebirth of a region of transplantation, it can be used.