Method for detecting bioanalytes using metal nanoshells

(57) Sensitivity is raised while facilitating the detection method of the living body analysis thing which uses summary Raman scattering of in vitro one and in vivo one. Solution means This invention the microscopic particles ("detailed shell") for using it in a living body perception use, the method of manufacturing them, and chemical and a living body analysis thing preferably, By surface amplification Raman light scattering, in order to detect by in vitro one and in vivo one, the method of using detailed shell is given. Desirable particles have a metal shell which encloses a non-conductivity core and its core. About a predetermined core and the material of shell, the ratio of the thickness (namely, radius) of the core to the thickness of a metal shell determines the maximum absorbance wavelength of particles. By controlling a core and the relative thickness of shell, the living body perception metal detailed shell which absorbs the light in the predetermined wavelength of an infrared region from the outside of the purple of an electromagnetic spectrum is formed. The field of particles can induce the amplified SERS signal peculiar to a related analysis thing. In the form of a certain enforcement, a biomolecule is combined with a metal shell and conformation change or the SERS signal of a reaction product is detected.