High resolution dna sequencing method using low viscosity medium

[Subject] The present invention should offer how to have been improved for attaining single base decomposition of DNA sequence determination fragmentation, and equipment by giving the low viscosity medium with which it is loaded easily and which is removed from a capillary electrophoresis tube. [Solution means] The DNA fragmentation in the mixture of DNA sequence determination is the nucleotide sequence of the target polynucleotide separated with a size the method of determining, and it: this gentleman method, The process of separating the above-mentioned DNA fragmentation by the capillary electrophoresis in the inside of denaturation solution containing the medium for a capillary electrophoresis is included, and the method by which single base decomposition is attained to the fragmentation of about 300 base length at least is offered here. Moreover, the low viscosity of solution enables the quick charge to the capillary tube of this kind of solution, and re-charge. [Selection figure] Nothing