Method for isolating and purifying viruses from plant sources

(57) Summary book invention relates to the method for isolating and refining the virus, protein, and peptide of concern from the vegetable host who can apply on a large scale. Furthermore, the present invention offers the method that it is more efficient than the method indicated by the prior art since the virus, protein, and peptide of concern are isolated. Generally the method of isolating the virus, protein, and peptide of concern, Homogenize a plant, make green juice, adjust the pH, and green juice is heated, 1 or two or more cycles of centrifugality, re-suspension, and extra filtration separate the target kind of either a virus, or protein/peptide from other ingredients of green juice, And it includes refining a viral particle by a method like PEG precipitation, or refining protein and peptide by chromatography and/or a method like salt precipitation in the last.