High-density lipoprotein assay device and method

Test device (14) for measuring serum cholesterol associated with high density lipoproteins (HDL) in a blood fluid sample also containing low density lipoproteins (LDL) or very low-density lipoprotein (VLDL), the device comprising: a .a sample distribution matrix (26) for distributing the blood fluid sample; bA element (74) containing an immobilized reagent effective to selectively bind and / or remove non-HDL lipoproteins from the fluid sample; c.una and HDL test pad (64) which can be assayed HDL concentration, in fluid communication with said reagent pad (74); wherein said reagent pad (74) can be placed in fluid communication with said sample distribution matrix (26).