Methods for producing modified glycoproteins

[Subject] Offer of a method and a constituent which makes the glycoprotein produced by the animal cell (especially human cell) and the same glycosylation protein (glycoprotein) produce. [Means for Solution] A lower eucaryote which usually generates high mannose of N-グリカン content containing a fungus of a single cell and a multicell is Man. 5 GlcAc 2 -- it is changed in order to generate other structures in alignment with N-グリカン [like] or a human glycosylation pathway. A system which does not reveal specific enzyme which generates the feature of complex structure which is not the request of fungus glycoprotein, A system which reveals exogenous enzyme chosen so that it may be whether to target a cell organelle, when it has the optimal activity under conditions which exist in a fungus when asking for activity or optimal activity is attained. [Chosen drawing] Nothing