Met/fret labelled amplification oligonucleotides and methods using them

(-- A summary 57) The nucleic acid amplification oligonucleotide which can be (correction -- owner) solution means straight chain shape, a hairpin primer, or a block oligonucleotide and which carried out the sign is offered. The sign of the oligonucleotide of the present invention is carried out in the donor and/or receptor portion of a molecular energy transportation pair. This portion can be fluorophore as the fluorescence energy released by the donor is absorbed by the receptor. A donor portion may be fluorophore which shows a fluorescence on a different wavelength, and a receptor may be a quencher. The oligonucleotide of the present invention is formed so that a donor portion and a receptor portion may be included in an amplification product. The present invention offers again the method and kit which detect an amplification product directly using a nucleic acid amplification primer. Effect If the disposal by エキソ nuclease or a special temperature is used when using the straight-chain-shape primer which carried out the sign, it will become unnecessary to separate the primer which was not incorporated.