Dual selection cassette and plasmids containing same

(57) The 1st and the 2nd DNA fragment (each has connected with the own promoter functionally) in which summary book invention has homogeny in a living thing viral vector, positive selector genes, and negative selector genes, And the double selection cassette (DSC) containing at least one unique restriction enzyme part (URES) or a tab-control-specification homologous recombination part is offered. The present invention offers plasmid pN/P which contains another positive selectable-markers gene, an initiation site of replication, and a double selection cassette again. This double selection cassette and a pN/P plasmid can be used for manufacturing a eucaryotic organism transgenics vector, without using the special bacteria stock which permits the duplicate of a plasmid including the deficit of the double recombination phenomenon or initiation site of replication coordinated in time. This method raises effectively the plasmid for which it does not ask, the desired plasmid to a vector structure, and the rate of a vector structure. Furthermore, the present invention offers the method of inventing a eucaryotic organism viral vector library.