Device for enhancing transdermal flux of sampled agents

Apparatus (10) for piercing the stratum corneum and withdrawing body fluid therethrough for sampling of a substance in the fluid, the apparatus comprising a piercing member (2) having a plurality of microblades (4) for piercing the stratum horny, presenting the microblades (4) sufficient to pierce the stratum corneum to a depth of between 25 and 400 m length, and being arranged in a part of the apparatus (48) that comes into contact with the stratum corneum, wherein the element punching (2) a plurality of passageways (8) fluid flow therethrough, the apparatus further comprising a device for application of partial vacuum (11, 12) connected to the piercing member (2) for applying a vacuum partial in a range of 1, 0x104 and 8, 1x104 Nm-2 (from 0, 1 and 0, 8 atmospheres) for a period of 2 to 30 seconds through passageways (8) of fluid flow stratum corneum perforated pararetirar the characterized body fluid in that the application of partial vacuum comprises a syringe body (122) having an open connection to the stratum corneum end, and a piston-rod (124) having a passage air (133) extending therethrough, wherein the syringe body (122) also contains a unit (123) spring biased plunger having the piercing member (2) mounted thereon.