Isolation of single stranded nucleic acids

The invention relates to methods PAR ALA EASY SEPARATION MATERIAL nucleic acid ONE ROW OF A MATERIAL OF THESE TWO nucleic acid ROWS IN A SAMPLE CONTAINING THE TWO. By the appropriate choice of at least one chaotropic agent, preferably a guanidine salt, to a selected CONCENTRATION AND OTHER CONDITIONS SUITABLE SUCH AS chelating agents, pH and the like, can be linked MATERIAL TWO ROWS to a solid phase AS PARTICLES sILICA, WHILE THE MATERIAL ONE RANK not bind under these circumstances. IF SEPARATED SILICA PARTICLE SAMPLE, IS REMOVED MATERIAL TWO ROWS nucleic acid. Elute CAN EASILY SILICA PARTICLE. IN A SECOND STEP ONE MATERIAL CAN UNITE FILA A SOLID PHASE selected a different set of conditions. The particles can be separated again from the sample and elute AND MATERIAL CAN ONE ROW. FOR EACH OF THE SEPARATION EFFICIENT, you can repeat the process. AFTER SEPARATION OF THE TWO TYPES nucleic acid can be amplified ANY KIND OF THEM. Amplification methods PRESENTED IN WHICH ARE NOT NECESSARY DATA MATERIAL sequences amplified. A PRIMER IN THESE METHODS HAVE A REASON FOR AMPLIFICATION AND HYBRIDIZATION OCCASION OF RANDOM.