Mesoscale polynucleotide amplification devices

[Subject] Even if the concentration of polynucleotide is very low, it can be detected, and the analysis apparatus from which an analysis result is moreover obtained promptly is offered. [Solution means] It is the method of amplifying the polynucleotide in a sample by performing a polynucleotide amplification reaction, It has at least one middle-scale section size within the limits of 0.1*1, 000 microns, The solid board formed so that it might have at least one polynucleotide amplification reaction chamber which consists of at least one reagent for amplification living body outside of the above-mentioned polynucleotide, The step which prepares the equipment which consists of the above-mentioned reaction chamber and at least one port which 連通 (ed) in order to introduce the above-mentioned sample into the above-mentioned reaction chamber, The step which leads the liquid containing at least one reagent and blocking reagent for the amplification in the interior of a room of a polynucleotide sample and a polynucleotide sample in the above-mentioned reaction chamber is included through the above-mentioned port. [Selection figure] Fig. 22