Two-photon laser scanning microscopy

A scanning microscope laser (10) producing a MOLECULAR EXCITATION IN A MATTER OF WHITE by simultaneous two-photon absorption to thereby provide a RESOLUTION THREE-DIMENSIONAL INTRINSIC. Fluorophores having absorption PHOTON EASY ON THE RANGE short wavelengths (UV or visible) they ARE EXCITED BY A SUCCESSION OF PULSES sub-picosecond STRONGLY FOCUSED LASER LIGHT (14) RANGE OF Wavelengths relatively long (RED O INFRARED). ABSORB THE fluorophore AROUND HALF WAVELENGTH laser to produce images FLUORESCENT CELL ALIVE AND OTHER OBJECTS MICROSCOPIC. The fluorescent emission from fluorophore INCREASES THE quadratically with the excitation SO BASED LIGHT FOCUSING STRONGLY laser (14), fluorescence photobleaching ARE WELL AS NEAR THE FOCAL PLANE BOUND (18). This feature provides DEPTH TO RESOLUTION CAMPOCOMPARABLE CAUSED BY EXPLORATION Microscopic lasing confocal and reduces photobleaching. EXPLORATION OF LASER BEAM (14), a scanning microscope laser (10) enables imaging by collecting fluorescence excited by two photons from each point on the object SCANNED WHILE STILL MEETS THE REQUIREMENT FOR VERY HIGH INTENSITY EXCITEMENT OBTAINED BASED LASER BEAM FOCUS (14) cOMPRESS AND BEAM PULSE TIME. THE IMPULSE FOCUSED THREE-DIMENSIONAL also provide spatially resolved PHOTOCHEMISTRY which is particularly useful in releasing effector molecules photolytic cage.